New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	4195732 =	93 (0.980)	3 (0.030)	3/246	NT	3.6%	coding (368/1896 nt)	thiC	phosphomethylpyrimidine synthase
	?	NC_000913	4195811 =	74 (0.830)					coding (289/1896 nt)	thiC	phosphomethylpyrimidine synthase
Rejected: Frequency below/above cutoff threshold.

GGTCTACGACACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4195884‑4195732 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cggctggcGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGACCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTACCGCCAATTAGCGTCGGGCTAAGCTGGATCTC  >  NC_000913/4195811‑4195958                                                                                                                                                                                                                                                                                                               GGTCTACGACACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGC                                                                                                                                                          >  3:356308/1‑149   TCTACGACACCTCCGGCCCGTATGGTGATCCGGAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTTAAGCGCGGCTG                                                                                                                                                        <  5:141842/149‑1   TCTACGACACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTG                                                                                                                                                        >  5:283009/1‑149     TACGACACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTGG                                                                                                                                                       >  1:10053/1‑148       CGACACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTGGC                                                                                                                                                      <  4:174856/147‑1       CGACACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTGGC                                                                                                                                                      <  4:353468/147‑1           ACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGG                                                                                                                                                           <  3:284867/138‑1           ACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGG                                                                                                                                                           >  4:284867/1‑138                CGGCCCGTATGGTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCT                                                                                                                                                         >  8:235219/1‑135                CGGCCCGTATGGTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCT                                                                                                                                                         <  7:235219/135‑1                                                                             CAGCCGTGGATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCCTACGGGCCGGAGGTGTCGTAGA                                                                              >  6:268189/1‑149                                                                                  GTGGATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGACCGG                                                                          >  3:313598/1‑148                                                                                          ATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTGGCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGACCGGAATCGCTTC                                                                 <  4:313598/149‑1                                                                                                                                                        GCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGACCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTACCGCCAATTAGCGTCGGGCTAAGCTGGATCT   >  3:157096/1‑149                                                                                                                                                        GCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGACCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTACCGCCAATTAGCGTCGGGCTAAGCTGGATC    >  6:179584/1‑148                                                                                                                                                        GCGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGACCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTACCGCCAATTAGCGTCGGGCTAAGCTGGAT     <  4:380984/147‑1                                                                                                                                                         CGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGACCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTACCGCCAATTAGCGTCGGGCTAAGCTGGATCTC  <  5:195583/149‑1                                                                                                                                                                                                                                                                                                               GGTCTACGACACCTCCGGCCCGTATGGTGATCCGCAGATTGCCATTAACGTGCAGCAAGGGCTGGCAAAACTACGCCAGCCGTGGATCGATGCGCGCGGCGATACCGAAGAACTTACCGTGCGCAGTTCCGATTACACTAAAGCGCGGCTGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4195884‑4195732 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cggctggcGTAGTTTTGCCAGCCCTTGCTGCACGTTAATGGCAATCTGCGGATCACCATACGGGCCGGAGGTGTCGTAGACCGGAATCGCTTCGTTTTCTTCGTACTGCGGCTGTTCTTTGCTACCGCCAATTAGCGTCGGGCTAAGCTGGATCTC  >  NC_000913/4195811‑4195958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.