Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 953,509 T→C 5.0% T16A (ACC→GCC)  pflB ← formate C‑acetyltransferase 1, anaerobic; pyruvate formate‑lyase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913953,5090TC 5.0% 250.0 / 2.4 79T16A (ACC→GCC) pflBformate C‑acetyltransferase 1, anaerobic; pyruvate formate‑lyase 1
Reads supporting (aligned to +/- strand):  ref base T (38/37);  new base C (2/2);  total (40/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

CAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAGGTAAATGACCCAGTATGTCAACC  >  NC_000913/953363‑953655
                                                                                                                                                  |                                                                                                                                                  
cAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTGCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTa                                                                                                                                                   >  5:407644/1‑148 (MQ=255)
  gTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTaaaa                                                                                                                                                >  7:250483/1‑149 (MQ=255)
  gTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTaaaa                                                                                                                                                <  7:38052/149‑1 (MQ=255)
       aCGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCATGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTc                                                                                                                                           <  8:358060/149‑1 (MQ=255)
        cGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGCAAAACCTTcc                                                                                                                                          >  8:215728/1‑149 (MQ=255)
        cGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGCAAAACCTTcc                                                                                                                                          >  8:209762/1‑149 (MQ=255)
          cccttcATTACTTTTTTCCAAATGGTGGGGGTTGGTTTACTAGCGCCATGCATGTATGTATCGGCACCCTTGTTAGGTTGGTATTTTTTCCTTATGTATTCACGGTCGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCa                                                                                                                                        <  6:411624/144‑1 (MQ=255)
           cTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTTCTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAg                                                                                                                                       >  4:148119/1‑149 (MQ=255)
           cTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAg                                                                                                                                       >  6:429212/1‑149 (MQ=255)
            ttCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACGTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAgg                                                                                                                                      >  3:33760/1‑149 (MQ=255)
                aTTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCtgt                                                                                                                                  <  1:313625/149‑1 (MQ=255)
                aTTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCtgt                                                                                                                                  >  2:262219/1‑149 (MQ=255)
                 ttACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCtgtg                                                                                                                                 <  1:372390/149‑1 (MQ=255)
                  tACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTgg                                                                                                                                >  5:368149/1‑149 (MQ=255)
                     tttGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCt                                                                                                                              >  7:158986/1‑148 (MQ=255)
                       tGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTaa                                                                                                                            <  8:285046/148‑1 (MQ=255)
                        gTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAAc                                                                                                                           <  1:262219/148‑1 (MQ=255)
                           ccACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACtttt                                                                                                                       >  5:321857/1‑149 (MQ=255)
                            cacaGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGCAAAACCTTCCCAGGCTGTGGCTAACTTTTc                                                                                                                      <  7:215728/149‑1 (MQ=255)
                            cacaGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGCAAAACCTTCCCAGGCTGTGGCTAACTTTTc                                                                                                                      <  7:209762/149‑1 (MQ=255)
                               aGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCAtt                                                                                                                   <  8:158986/149‑1 (MQ=255)
                                 ggtggtggtCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACACTCGTAAGGAGTGTATTTTTTCTGAATGAAGTAACGGTCTTTTAATTCATTCTGCCAGTCACCGTTGGTAAAACCTGACCAGGCTGTGGCTAAGTTTTCAtt                                                                                                                   >  7:303415/1‑147 (MQ=255)
                                   tggtggtCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTcc                                                                                                                                          <  2:380372/122‑1 (MQ=255)
                                   tggtggtCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTcc                                                                                                                                          >  1:380372/1‑122 (MQ=255)
                                        gtCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTaa                                                                                                                 <  3:305122/142‑1 (MQ=255)
                                        gtCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTaa                                                                                                                 >  4:305122/1‑142 (MQ=255)
                                           gCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACAt                                                                                                       <  7:94574/149‑1 (MQ=255)
                                              tCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGt                                                                                                     >  1:289346/1‑148 (MQ=255)
                                               cAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGt                                                                                                     <  7:133563/147‑1 (MQ=255)
                                                aGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTaa                                                                                                   <  6:321857/148‑1 (MQ=255)
                                                        cagccagGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACacctac                                                                                            <  4:127366/147‑1 (MQ=255)
                                                         agccagGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTaa                                                                                                   <  7:363808/139‑1 (MQ=255)
                                                         agccagGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTaa                                                                                                   >  8:363808/1‑139 (MQ=255)
                                                            cagGAAGGACTCGTAACCCTAGTACGGAGTGTAGTTTTTCTGAATTAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTTGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACcttctt                                                                                      <  8:208403/149‑1 (MQ=255)
                                                                        gTCACACTCGTACGGAGTGTAGTTTTTCTTAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGTAttttt                                                                          <  8:303415/149‑1 (MQ=255)
                                                                         tCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGAttttt                                                                          >  6:183396/1‑148 (MQ=255)
                                                                          cACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTttta                                                                        <  6:407644/149‑1 (MQ=255)
                                                                            cccTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAATCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTtttatt                                                                      <  5:126244/149‑1 (MQ=255)
                                                                             ccTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTtttatt                                                                      >  1:4835/1‑148 (MQ=255)
                                                                                 gTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACt                                                                  <  1:348745/148‑1 (MQ=255)
                                                                                  tACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAATCCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTACGTGGATTTTTTATTTACTGc                                                                <  5:158046/149‑1 (MQ=255)
                                                                                    cGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAAGCATCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTATTTATTAACTGCg                                                               >  2:78243/1‑148 (MQ=255)
                                                                                       aGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACt                                                           <  2:279114/149‑1 (MQ=255)
                                                                                           tAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTc                                                         >  1:4664/1‑147 (MQ=255)
                                                                                           tAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTc                                                         <  2:4664/147‑1 (MQ=255)
                                                                                              tttttCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGAt                                                                              >  1:180845/1‑123 (MQ=255)
                                                                                              tttttCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGAt                                                                              <  2:180845/123‑1 (MQ=255)
                                                                                                    tgaatgaaGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCAtt                                               >  7:103071/1‑148 (MQ=255)
                                                                                                     gaatgaaGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTaa                                             >  7:12392/1‑149 (MQ=255)
                                                                                                      aatgaaGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTaa                                             <  5:429212/148‑1 (MQ=255)
                                                                                                      aatgaaGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAAt                                            >  7:154482/1‑149 (MQ=255)
                                                                                                         gaaGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCATCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAAtggt                                         >  5:200631/1‑149 (MQ=255)
                                                                                                             tCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCTGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTc                                     <  7:334287/149‑1 (MQ=255)
                                                                                                             tCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTc                                     >  4:117703/1‑149 (MQ=255)
                                                                                                                 ggACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGttt                                 >  4:200571/1‑149 (MQ=255)
                                                                                                                    cGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCa                              <  2:289346/149‑1 (MQ=255)
                                                                                                                     gTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCAc                             >  8:74678/1‑149 (MQ=255)
                                                                                                                     gTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCAc                             >  6:221181/1‑149 (MQ=255)
                                                                                                                        tACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGc                           <  8:250483/148‑1 (MQ=255)
                                                                                                                          cTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGTACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAg                         <  3:339797/148‑1 (MQ=255)
                                                                                                                          cTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAgg                        <  3:200571/149‑1 (MQ=255)
                                                                                                                          cTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTAGTCGTTTTc                               >  8:416020/1‑142 (MQ=255)
                                                                                                                          cTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTAGTCGTTTTc                               <  7:416020/142‑1 (MQ=255)
                                                                                                                           ttCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAgg                        >  7:259740/1‑148 (MQ=255)
                                                                                                                           ttCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAgg                        >  2:391992/1‑148 (MQ=255)
                                                                                                                           ttCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAgg                        >  7:362544/1‑148 (MQ=255)
                                                                                                                           ttCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAgg                        >  2:199013/1‑148 (MQ=255)
                                                                                                                           ttCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTGAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAgg                        >  4:167034/1‑148 (MQ=255)
                                                                                                                              aTTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAGGTaaa                    <  7:383664/149‑1 (MQ=255)
                                                                                                                                    ccAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAGGTAAATGa                 <  3:148119/146‑1 (MQ=255)
                                                                                                                                    ccAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAGGTAAATGAcc               >  4:228371/1‑148 (MQ=255)
                                                                                                                                    ccAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAGGTAAATGAcc               >  3:117936/1‑148 (MQ=255)
                                                                                                                                        tCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAGGTAAATGACCCAGTa          <  5:366988/149‑1 (MQ=255)
                                                                                                                                        tCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAGGTAAATGACCCAGTa          <  6:368149/149‑1 (MQ=255)
                                                                                                                                           ccTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCAt                                                >  8:106265/1‑108 (MQ=255)
                                                                                                                                           ccTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCAt                                                <  7:106265/108‑1 (MQ=255)
                                                                                                                                             tttGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATCGTGGTCGTTTTCACGCAGGTAAATGAACCAGTATGTc      >  6:14313/1‑148 (MQ=255)
                                                                                                                                               tGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAGGTAAATGACCCAGTATGTcaac   >  2:227589/1‑149 (MQ=255)
                                                                                                                                                 gTAAAACCTTCCCCGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAGGTAAATGACCCAGTATGTcaacc  <  2:322102/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CAGTTTAACGCCTTCCATTACTTTGTCCCACAGGGTGGTGGTCGCTTCAGTAGCGCCAGCCAGGAAGGACTCGTCACCCTCGTACGGAGTGTAGTTTTTCTGAATGAAGTCACGGACGTTTACTTCATTCTGCCAGTCACCTTTGGTAAAACCTTCCCAGGCTGTGGCTAACTTTTCATTAAGCTCGGACATGTAACACCTACCTTCTTAAGTGGATTTTTTATTTACTGCGTACTTCGACAACCATTAATGGTGGTCGTTTTCACGCAGGTAAATGACCCAGTATGTCAACC  >  NC_000913/953363‑953655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑