Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,376,207 C→A 8.0% A58A (GCC→GCA ycjR → putative TIM alpha/beta barrel enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,376,2070CA 8.0% 317.4 / 10.4 97A58A (GCC→GCAycjRputative TIM alpha/beta barrel enzyme
Reads supporting (aligned to +/- strand):  ref base C (38/51);  new base A (3/5);  total (41/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GTTCTTTCCGGAAAACATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACC  >  NC_000913/1376060‑1376351
                                                                                                                                                   |                                                                                                                                                
gTTCTTTCCGGAAAACATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGGTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCt                                                                                                                                                 <  6:154764/149‑1 (MQ=255)
   cTTTCCGGAAAACATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCg                                                                                                                                              >  7:264347/1‑149 (MQ=255)
    tttCCGGAAAACATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCgg                                                                                                                                             >  4:31505/1‑149 (MQ=255)
      tCCGGAAAACATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGGTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCg                                                                                                                                              <  1:293894/146‑1 (MQ=255)
       ccGGAAAACATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTg                                                                                                                                           <  6:182838/148‑1 (MQ=255)
        gggACAACATTCTGGAGTAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACGGGTGACCACCGCCTGCGGTgg                                                                                                                                          <  5:19816/147‑1 (MQ=255)
        cGGAAAACATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTgg                                                                                                                                          <  7:84589/148‑1 (MQ=255)
         ggAAAACATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACCACATCGAAGAAGTCAACGCGGCGATCAAAGAAACCGGGTTACCGGTGACCACCGCCTGCGGTGGc                                                                                                                                         >  3:335229/1‑148 (MQ=255)
         ggAAAACATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCt                                                                                                                                        <  4:348598/149‑1 (MQ=255)
          gAAAACATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTa                                                                                                                                       <  3:31505/149‑1 (MQ=255)
             aaCATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGa                                                                                                                                    <  7:271632/149‑1 (MQ=255)
             aaCATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCAGGCGGTggtcaccg                                                                                                                                    <  7:280377/149‑7 (MQ=255)
                 ttCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACgga                                                                                                                                <  2:425277/149‑1 (MQ=255)
                      gagaAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGCTTGACGGCAAACTGCTCGTTAACAACATCGAAGAAGTCAAAGCGGCGAACAAAGAAACCGGTTTACCGGTGACCACCGCCTGGGGTGGCTATGACggatcgat                                                                                                                           >  1:384022/1‑149 (MQ=255)
                       agaAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGAtt                                                                                                                          <  7:367248/149‑1 (MQ=255)
                            tttCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGa                                                                                                                     >  1:191092/1‑149 (MQ=255)
                            tttCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGa                                                                                                                     <  4:145242/149‑1 (MQ=255)
                                gTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACttt                                                                                                                 <  3:253782/149‑1 (MQ=255)
                                gTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGACAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACttt                                                                                                                 <  3:18543/149‑1 (MQ=255)
                                   atatCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTAt                                                                                                               <  8:54834/148‑1 (MQ=255)
                                    tatCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACttatcg                                                                                                              >  5:315939/1‑144 (MQ=255)
                                      tCATAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGaa                                                                                                           <  6:402494/149‑1 (MQ=255)
                                       cAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCg                                                                                                             >  7:221955/1‑146 (MQ=255)
                                        aaaGAGATGGTCTTAGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAaga                                                                                                         <  3:46390/149‑1 (MQ=255)
                                        aaaGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGaa                                                                                                           >  7:275169/1‑147 (MQ=255)
                                        aaaGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAaga                                                                                                         <  2:242734/149‑1 (MQ=255)
                                        aaaGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAaga                                                                                                         <  4:184966/149‑1 (MQ=255)
                                        aaaGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAaga                                                                                                         <  3:71997/149‑1 (MQ=255)
                                               tGGGCTTCGATGGTTTTGAGATTGACGGCAATCTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGcgtcgt                                                                                                  >  1:161367/1‑149 (MQ=255)
                                                 ggCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCgtcgtc                                                                                                 <  4:105924/148‑1 (MQ=255)
                                                 ggCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCt                                                                                                <  7:199454/149‑1 (MQ=255)
                                                   cTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCtt                                                                                               >  5:143708/1‑148 (MQ=255)
                                                     tCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAAt                                                                                            >  7:35115/1‑149 (MQ=255)
                                                      cGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTaa                                                                                             <  5:24885/147‑1 (MQ=255)
                                                      cGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAAt                                                                                            <  6:284426/148‑1 (MQ=255)
                                                      cGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTATCGAAGAGCGTCGTCTTAATg                                                                                           <  6:315939/148‑1 (MQ=255)
                                                       gATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATgg                                                                                          >  7:55550/1‑149 (MQ=255)
                                                       gATGGTTTAGAGATAGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATgg                                                                                          <  4:71721/149‑1 (MQ=255)
                                                        aTGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGc                                                                                         >  5:389944/1‑149 (MQ=255)
                                                        aTGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGc                                                                                         <  8:221955/149‑1 (MQ=255)
                                                                gagaTTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGc                                                                                  <  4:381157/148‑1 (MQ=255)
                                                                 agaTTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGTCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCa                                                                                 <  8:275169/148‑1 (MQ=255)
                                                                    ttGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGAGGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATc                                                                             <  2:80442/149‑1 (MQ=255)
                                                                      gACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATc                                                                             >  3:146375/1‑147 (MQ=255)
                                                                         ggCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGc                                                                                         <  7:404953/132‑1 (MQ=255)
                                                                         ggCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGc                                                                                         >  8:404953/1‑132 (MQ=255)
                                                                         ggCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAgcg                                                                        >  8:21167/1‑149 (MQ=255)
                                                                            aaaCTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTaa                                                                                             >  4:212957/1‑125 (MQ=255)
                                                                            aaaCTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTaa                                                                                             <  3:212957/125‑1 (MQ=255)
                                                                            aaaCTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCAt                                                                     >  5:239266/1‑149 (MQ=255)
                                                                              aCTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACAGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATtc                                                                   <  7:190920/149‑1 (MQ=255)
                                                                                  ctgGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCg                                                                <  8:35115/148‑1 (MQ=255)
                                                                                     gTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGaa                                                              <  5:308193/147‑1 (MQ=255)
                                                                                     gTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGaa                                                              <  1:229211/147‑1 (MQ=255)
                                                                                      ttAACAACATCGAAGAAGCCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAgcg                                                           >  3:151672/1‑149 (MQ=255)
                                                                                              aTCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCTACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATAGAGCGCAATCTCGAAGCGATGGCAg                                                    >  1:163416/1‑148 (MQ=255)
                                                                                                   ataattCAAAGGGGCTTTCAAAGAAACTGGTTTATCGGTTACCACGGTATGTGGTGGTTATGAGGTTTGGTTTTGTGATTTTATCTAATTGCTTTGTTTTAATGGCTTAAAGAATATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTg                                               <  6:143708/143‑1 (MQ=255)
                                                                                                    gaagTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTaa                                                                                             >  8:276138/1‑101 (MQ=255)
                                                                                                    gaagTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTaa                                                                                             <  7:276138/101‑1 (MQ=255)
                                                                                                    gaagTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTggg                                             <  6:239266/149‑1 (MQ=255)
                                                                                                    gaagTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTggg                                             <  8:264347/149‑1 (MQ=255)
                                                                                                     aagTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGc                                            <  2:191092/149‑1 (MQ=255)
                                                                                                        tCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCgg                                          >  6:114173/1‑148 (MQ=255)
                                                                                                          aaaGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGCGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTa                                        >  3:289288/1‑148 (MQ=255)
                                                                                                          aaaGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATtctc                                                                 >  7:204990/1‑123 (MQ=255)
                                                                                                          aaaGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATtctc                                                                 <  8:204990/123‑1 (MQ=255)
                                                                                                          aaaGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAgcgc                                                          <  7:181901/130‑1 (MQ=255)
                                                                                                          aaaGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAgcgc                                                          >  8:181901/1‑130 (MQ=255)
                                                                                                          aaaGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTAGAAGCGCTGGCAGAAGTGGGCGGTa                                        >  8:159287/1‑148 (MQ=255)
                                                                                                              cggcgATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGc                                   <  4:146375/149‑1 (MQ=255)
                                                                                                              cggcgATCAAAGAAACCGGTTTACCGGTGACCACCCCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCGGGCAGAAGTGGGCGGTAAAgg                                    >  2:341572/1‑148 (MQ=255)
                                                                                                                gcgATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCAt                                 <  8:270525/149‑1 (MQ=255)
                                                                                                                 cgATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGGCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCAtc                                <  5:114173/149‑1 (MQ=255)
                                                                                                                  gATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTGAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCAtcg                               >  3:243414/1‑149 (MQ=255)
                                                                                                                   aTCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTaa                                                                                     <  5:59715/94‑1 (MQ=255)
                                                                                                                   aTCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTaa                                                                                     >  6:59715/1‑94 (MQ=255)
                                                                                                                    tCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCAtcgtc                             >  2:64202/1‑149 (MQ=255)
                                                                                                                    tCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATAGAGAGCATTCTCGAATCGCTGGCAGAAGTGGGCGGTAAAGGAAtcgtc                             >  6:156298/1‑149 (MQ=255)
                                                                                                                      aaaGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGc                                                                                         <  7:264363/87‑1 (MQ=255)
                                                                                                                      aaaGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGc                                                                                         >  8:264363/1‑87 (MQ=255)
                                                                                                                        aGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTc                         >  6:181355/1‑149 (MQ=255)
                                                                                                                        aGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTc                         <  5:153331/149‑1 (MQ=255)
                                                                                                                        aGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTc                         <  2:161367/149‑1 (MQ=255)
                                                                                                                           aaCCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCgg                      >  5:298861/1‑149 (MQ=255)
                                                                                                                            aCCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGc                     >  3:24482/1‑149 (MQ=255)
                                                                                                                                    aCCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTggg             >  3:256628/1‑149 (MQ=255)
                                                                                                                                      cGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTgggg            >  7:236432/1‑148 (MQ=255)
                                                                                                                                      cGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGc           <  6:389944/149‑1 (MQ=255)
                                                                                                                                        atagccaccGCAGGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGCTCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTgg              <  5:343976/140‑1 (MQ=255)
                                                                                                                                        atagccaccGCAGGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAg                                     <  7:364691/117‑1 (MQ=255)
                                                                                                                                        atagccaccGCAGGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAg                                     >  8:364691/5‑121 (MQ=255)
                                                                                                                                        atagccaccGCAGGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTgg              >  6:113487/5‑144 (MQ=255)
                                                                                                                                        atagccaccGCAGGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTgg              <  5:113487/140‑1 (MQ=255)
                                                                                                                                        atagccaccGCAGGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTgg              >  6:343976/5‑144 (MQ=255)
                                                                                                                                            ccaccGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGttt     <  4:256628/149‑1 (MQ=255)
                                                                                                                                              accGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTAc   <  8:55550/149‑1 (MQ=255)
                                                                                                                                               ccGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATTGTCGTTCCGGCTGCGTGGGGCATGTTTAcc  >  7:156247/1‑149 (MQ=255)
                                                                                                                                                  ccTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTgg              >  3:14399/1‑134 (MQ=255)
                                                                                                                                                  ccTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTgg              <  4:14399/134‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GTTCTTTCCGGAAAACATTCTGGAGAAATTTCGTTATATCAAAGAGATGGGCTTCGATGGTTTTGAGATTGACGGCAAACTGCTGGTTAACAACATCGAAGAAGTCAAAGCGGCGATCAAAGAAACCGGTTTACCGGTGACCACCGCCTGCGGTGGCTATGACGGATGGATTGGCGACTTTATCGAAGAGCGTCGTCTTAATGGCTTAAAGCAGATCGAGCGCATTCTCGAAGCGCTGGCAGAAGTGGGCGGTAAAGGCATCGTCGTTCCGGCTGCGTGGGGCATGTTTACC  >  NC_000913/1376060‑1376351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: