Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,794,341 T→A 5.8% D271V (GAT→GTT)  btuC ← vitamin B12 ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,794,3410TA 5.8% 205.8 / 3.3 69D271V (GAT→GTT) btuCvitamin B12 ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (26/37);  new base A (2/2);  total (28/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

CAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGCTGCCACCAGCACATTGCGCCAGAACCACAGGGGTAAACCCAGTTGCCG  >  NC_000913/1794197‑1794489
                                                                                                                                                |                                                                                                                                                    
cAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGt                                                                                                                                                  <  7:290246/149‑1 (MQ=255)
cAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGt                                                                                                                                                  <  8:141518/149‑1 (MQ=255)
   aTAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTa                                                                                                                                                >  6:143877/1‑148 (MQ=255)
   aTAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTa                                                                                                                                                >  4:267237/1‑148 (MQ=255)
    tAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTaaa                                                                                                                                              <  3:187879/149‑1 (MQ=255)
    tAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTaaa                                                                                                                                              <  6:306025/149‑1 (MQ=255)
    tAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTaaa                                                                                                                                              <  3:337979/149‑1 (MQ=255)
     aaaCACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAAc                                                                                                                                             >  1:187635/1‑149 (MQ=255)
            ggCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACaa                                                                                                                                      >  2:57240/1‑149 (MQ=255)
                 aCCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAACCCACACAACCg                                                                                                                                   >  3:82679/1‑147 (MQ=255)
                  ccTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATCCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGAAATACGCGATGATCGGTTAAACCACACAACCg                                                                                                                                   >  3:103526/1‑146 (MQ=255)
                   cTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACGGgaga                                                                                                                               <  4:101250/149‑1 (MQ=255)
                   cTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGgaga                                                                                                                               <  2:181650/149‑1 (MQ=255)
                   cTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGgag                                                                                                                                >  7:113240/1‑148 (MQ=255)
                    tAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGaa                                                                                                                              >  5:14242/1‑149 (MQ=255)
                      aCGTTGCGGTGACCACGCCAATAGCCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAat                                                                                                                             <  3:136596/148‑1 (MQ=255)
                       cGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAatat                                                                                                                           <  6:14242/149‑1 (MQ=255)
                            cGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATgggg                                                                                                                       <  5:143877/148‑1 (MQ=255)
                            cGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGAGCTAGCCACTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCTGAGAATATgggg                                                                                                                       <  5:107724/148‑1 (MQ=255)
                              gTGACCACGCCTATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAACAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGAt                                                                                                                    <  4:216373/149‑1 (MQ=255)
                               tGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCTGCCAGCAGCTTTGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGATc                                                                                                                   <  4:142954/149‑1 (MQ=255)
                               tGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGATc                                                                                                                   <  3:267237/149‑1 (MQ=255)
                                 aCCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGATCAc                                                                                                                 <  2:413872/149‑1 (MQ=255)
                                   cACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCCCGCTACAATATCGGCCAGCAGCAATGCACTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGATCAc                                                                                                                 >  4:24873/1‑147 (MQ=255)
                                   aaagCCAATAGGCAGATCTGCGGCAGCTAATGACAGGCGCGCTACAATATCGGACAGCAGCAATGCGCTCGCCCCTGACAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAAcc                                                                                                                                    <  6:397893/125‑1 (MQ=255)
                                     cGCCATTAGGCAGCTCTGCGGCAGCTAATTCCAGGCGCGCTACAATATCGGCCAGCAGGAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAACCCACACAACCGGAGAATATGGGGGATCACCAGa                                                                                                             <  2:405103/149‑1 (MQ=255)
                                             ggCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATaaa                                                                                                     <  6:16451/149‑1 (MQ=255)
                                             ggCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATaaa                                                                                                     <  8:113240/149‑1 (MQ=255)
                                                  ctctGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGa                                                                                                <  7:372655/149‑1 (MQ=255)
                                                     tGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGTGAAGTAATACGCGATGATCGGTTAAACCCCACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGAt                                                                                               <  5:203757/147‑1 (MQ=255)
                                                     tGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTACACCCCACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAg                                                                                             >  2:416285/1‑149 (MQ=255)
                                                     tGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGAt                                                                                               <  1:57240/147‑1 (MQ=255)
                                                     tGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAg                                                                                             >  5:177090/1‑149 (MQ=255)
                                                       cGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATa                                                                                              <  6:321985/146‑1 (MQ=255)
                                                       cGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATa                                                                                              <  6:71557/146‑1 (MQ=255)
                                                       cGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCa                                                                                           <  4:168794/149‑1 (MQ=255)
                                                       cGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCACTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAg                                                                                             <  3:24873/147‑1 (MQ=255)
                                                        ggCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGa                                                                                                             >  7:378276/1‑130 (MQ=255)
                                                        ggCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGa                                                                                                             <  8:378276/130‑1 (MQ=255)
                                                        ggCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCAc                                                                                          <  6:8704/149‑1 (MQ=255)
                                                            gCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGAACGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGc                                                                                      >  5:203165/1‑149 (MQ=255)
                                                            gCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGAACGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGc                                                                                      >  5:209414/1‑149 (MQ=255)
                                                                       gcgcgcTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATCGCACCCTCCAGCGCCacac                                                                           >  4:44403/1‑149 (MQ=255)
                                                                           gcTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGAc                                                                       >  1:326088/1‑149 (MQ=255)
                                                                                aaTATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGATTATGGGGGATCACCAGCCCAATAAAGCCGATAGCACCCGCAAGCGCCACACTGACGCCaa                                                                  >  2:381471/1‑149 (MQ=255)
                                                                                            cagcaATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGCGGATCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCCGCGCCACACTGCCGCCACCCCTCCCGCCgg                                                      >  1:338111/1‑149 (MQ=255)
                                                                                             agcaATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGt                                                     <  1:9122/149‑1 (MQ=255)
                                                                                               caATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACACCCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGCTAGCACCCGCCCGCGCCACACTGACGCCAACCATCCAGCCGGTCg                                                   >  7:440070/1‑149 (MQ=255)
                                                                                                 aTGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGCt                                                 <  3:11404/149‑1 (MQ=255)
                                                                                                  tGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGCTg                                                >  7:412054/1‑149 (MQ=255)
                                                                                                    cgcTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGCTGc                                               <  6:143579/148‑1 (MQ=255)
                                                                                                     gcTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGGTAAACCACACAACCGGAGAATATGGGGGCTCACCAGACCACTAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCCACCATCCCGCCGGTCGCTGcca                                             >  6:11812/1‑149 (MQ=255)
                                                                                                      cTCGCCCCTGCCAGCGCGCCGCAGGGAAGTAATACGCGATGATCGGATAAACCACACAACCGGAGAATATGGGGGATCACCCGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGCTGccac                                            >  4:302137/1‑149 (MQ=255)
                                                                                                       tCGCCCCTGCCAGCGCGCAGCCGGGATGTAATACGCGATGATCGGTTAACCCACACAACCGGAGATTATGGGGGATCACCAGACCAATAAAGCCGCTAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGCTGccac                                            >  3:140697/1‑148 (MQ=255)
                                                                                                       tCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGc                                                                                      >  8:94016/1‑106 (MQ=255)
                                                                                                       tCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGc                                                                                      <  7:94016/106‑1 (MQ=255)
                                                                                                       tCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGc                                                                                      >  6:127762/1‑106 (MQ=255)
                                                                                                       tCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGc                                                                                      <  5:127762/106‑1 (MQ=255)
                                                                                                       tCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGCTGccac                                            >  8:204028/1‑148 (MQ=255)
                                                                                                         gCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGCTGccacca                                          <  1:225932/148‑1 (MQ=255)
                                                                                                                     gcgcAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGCTGCCACCAGCACATTGCGCCa                             <  6:177090/149‑1 (MQ=255)
                                                                                                                     gcgcAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGCTGCCACCAGCACATTGCGCCa                             <  2:188000/149‑1 (MQ=255)
                                                                                                                           ccGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGCTGCCACCAGCACATTGCGCCAGAACca                       >  3:316495/1‑149 (MQ=255)
                                                                                                                             gggAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGCTGCCACCAGCACATTGCGCCAGAACca                       >  5:273486/1‑147 (MQ=255)
                                                                                                                              ggAAGTAATACGCGATGAACGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGCTGCCACCAGCACATTGCGCCAGAACCACAg                    <  6:203165/149‑1 (MQ=255)
                                                                                                                              ggAAGTAATACGCGATGAACGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGCTGCCACCAGCACATTGCGCCAGAACCACAg                    <  6:209414/149‑1 (MQ=255)
                                                                                                                                aaGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGCTGCCACCAGCACATTGCGCCAGAACCACAggg                  <  2:187635/149‑1 (MQ=255)
                                                                                                                                  gTAATACGCGAGGAGCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGCTGCCACCAGCACATTGCGCCAGAACCACAGGGGt                <  8:381902/149‑1 (MQ=255)
                                                                                                                                          cgATGAGCGGTTTAAGAAGAAATCCGGAGAATATGGGGGATCAGCAGACCAATAAAGACGATAGGACCCTCGAGGGCCACACTGACGCCAACCATCCAGAAGGTCGCTGCCACCAGCAGATTGCGCCAGTACCACTGGGGTAAAACCAg        <  2:338111/149‑1 (MQ=255)
                                                                                                                                           gatgatCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGCt                                                 >  2:218349/1‑107 (MQ=255)
                                                                                                                                           gatgatCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGCt                                                 <  1:218349/107‑1 (MQ=255)
                                                                                                                                           gatgatCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGCTGCCACCAGCACATTGCGCCAGAACCACAGGGGt                >  7:436675/1‑140 (MQ=255)
                                                                                                                                           gatgatCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGCTGCCACCAGCACATTGCGCCAGAACCACAGGGGt                <  8:436675/140‑1 (MQ=255)
                                                                                                                                           gatgatCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGCTGCCACCAGCACATTGCGCCAGAACCACAGGGGTAAACCCAGt       >  8:252452/1‑149 (MQ=255)
                                                                                                                                                tCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGACGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGCTGCCACCAGCACATTGCGCCAGAACCACAGGGGTAAACCCAGTTGcag  >  5:268245/1‑147 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
CAGATAAACACCGGCGCACCTAACGTTGCGGTGACCACGCCAATAGGCAGCTCTGCGGCAGCTAATGCCAGGCGCGCTACAATATCGGCCAGCAGCAATGCGCTCGCCCCTGCCAGCGCGCAGCCGGGAAGTAATACGCGATGATCGGTTAAACCACACAACCGGAGAATATGGGGGATCACCAGACCAATAAAGCCGATAGCACCCGCCAGCGCCACACTGACGCCAACCATCCAGCCGGTCGCTGCCACCAGCACATTGCGCCAGAACCACAGGGGTAAACCCAGTTGCCG  >  NC_000913/1794197‑1794489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: