Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,825,938 A→C 5.8% intergenic (‑313/+17) spy ← / ← astE periplasmic ATP‑independent protein refolding chaperone, stress‑induced/succinylglutamate desuccinylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,825,9380AC 5.8% 270.2 / 7.7 86intergenic (‑313/+17)spy/astEperiplasmic ATP‑independent protein refolding chaperone, stress‑induced/succinylglutamate desuccinylase
Reads supporting (aligned to +/- strand):  ref base A (37/44);  new base C (3/2);  total (40/46)
Fisher's exact test for biased strand distribution p-value = 6.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

GATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGTTC  >  NC_000913/1825792‑1826082
                                                                                                                                                  |                                                                                                                                                
gATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGAt                                                                                                                                                 <  7:135355/148‑1 (MQ=255)
 aTGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGAttt                                                                                                                                               <  4:86934/149‑1 (MQ=255)
       agTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCgg                                                                                                                                         <  2:60132/149‑1 (MQ=255)
           atgaGAGACTATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCACCTTAATACCCGCAGAATGATTTCTGCGGGTaa                                                                                                                                     <  5:22634/149‑1 (MQ=255)
           atgaGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTaa                                                                                                                                     <  1:145764/149‑1 (MQ=255)
             gagagaATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAg                                                                                                                                    >  3:48690/1‑148 (MQ=255)
             gagagaATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAg                                                                                                                                    >  5:343171/1‑148 (MQ=255)
              agagaATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTa                                                                                                                                  <  7:311262/149‑1 (MQ=255)
                    tatTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCt                                                                                                                            >  2:262544/1‑149 (MQ=255)
                      ttACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTa                                                                                                                          >  6:31933/1‑149 (MQ=255)
                          aacaCGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTAtttt                                                                                                                      >  1:349044/1‑149 (MQ=255)
                            cacGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTAtttttt                                                                                                                    >  4:62030/1‑149 (MQ=255)
                             acGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTCTTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTc                                                                                                                   <  5:24747/149‑1 (MQ=255)
                               gatgatTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGa                                                                                                                 <  2:98354/149‑1 (MQ=255)
                                 tgatTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAg                                                                                                                >  8:408901/1‑148 (MQ=255)
                                   atTTTGCAGAGATTATGAAGAACTATACCGGCTGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCAt                                                                                                             <  2:398774/149‑1 (MQ=255)
                                   atTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCa                                                                                                              <  5:139710/148‑1 (MQ=255)
                                        gCAGAGATTATGAAGAACTATACCGGATGAATGTTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAAt                                                                                                         <  6:244558/148‑1 (MQ=255)
                                          agagATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATccc                                                                                                      >  2:48052/1‑149 (MQ=255)
                                          agagATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATccc                                                                                                      >  5:261684/1‑149 (MQ=255)
                                            agaTTATGAAGAACTATACCTGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCcgc                                                                                                    <  8:18130/149‑1 (MQ=255)
                                              aTTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCcgcgc                                                                                                  <  6:131999/149‑1 (MQ=255)
                                                  tGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTaa                                                                                              >  2:277811/1‑149 (MQ=255)
                                                  tGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTaa                                                                                              >  2:260709/1‑149 (MQ=255)
                                                   gaagaaCTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGAAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAAt                                                                                             >  6:307185/1‑149 (MQ=255)
                                                         cTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAAc                                                                                       <  3:119136/149‑1 (MQ=255)
                                                         cTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAAc                                                                                       <  3:292554/149‑1 (MQ=255)
                                                         cTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAAATCATTCTGCGGGTattaag                                                                                                                                 <  1:104405/107‑6 (MQ=38)
                                                         cTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAAATCATTCTGCGGGTattaag                                                                                                                                 >  2:104405/1‑102 (MQ=38)
                                                         cTATACCGGATGACTGGGGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAAATTATAAGAAAGCATCTTTATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAAc                                                                                       <  6:188971/149‑1 (MQ=255)
                                                          tataCCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACg                                                                                      >  6:299131/1‑149 (MQ=255)
                                                              ccGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTAc                                                                                  <  2:349044/149‑1 (MQ=255)
                                                                 gATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCaa                                                                               >  8:70837/1‑149 (MQ=255)
                                                                 gATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCaa                                                                               >  8:174459/1‑149 (MQ=255)
                                                                         gTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGAtt                                                                         <  1:260709/147‑1 (MQ=255)
                                                                         gTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAg                                                                       <  1:48052/149‑1 (MQ=255)
                                                                          tGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAgg                                                                      <  5:299131/149‑1 (MQ=255)
                                                                          tGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTCTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAgg                                                                      <  4:48690/149‑1 (MQ=255)
                                                                            ataaataaAGCAAATAAACAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACACGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCAAACGCTACCAACGGATTAggt                                                                     >  3:34165/1‑147 (MQ=255)
                                                                             taaataaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGaa                                                                   <  5:72775/149‑1 (MQ=255)
                                                                                 taaaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAac                                                               <  7:408901/149‑1 (MQ=255)
                                                                                   aaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAacac                                                             <  8:318176/149‑1 (MQ=255)
                                                                                   aaGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAaca                                                              <  5:133668/148‑1 (MQ=255)
                                                                                       aaaTAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCcgc                                                                                                    <  1:316594/106‑1 (MQ=255)
                                                                                       aaaTAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCcgc                                                                                                    >  2:316594/1‑106 (MQ=255)
                                                                                       aaaTAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATAc                                                         <  7:425613/149‑1 (MQ=255)
                                                                                       aaaTAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATAc                                                         <  8:191684/149‑1 (MQ=255)
                                                                                        aaTAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGAGGGTAATTATTAGCTTATTTTTTCGAGCATTAATCCAGCGCGTAATCCCCACGCTACAAACGGATTAGGGAATAACACATAc                                                         >  7:303821/1‑148 (MQ=255)
                                                                                           aaCCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGAGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCAGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACtata                                                     >  5:313567/1‑149 (MQ=255)
                                                                                              cAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTAca                                                   >  8:78213/1‑148 (MQ=255)
                                                                                                ggATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTAcatca                                                <  7:78213/149‑1 (MQ=255)
                                                                                                ggATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTAcatca                                                >  7:414676/1‑149 (MQ=255)
                                                                                                 gATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTAcatcat                                               <  5:31933/149‑1 (MQ=255)
                                                                                                 gATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTAcatcat                                               >  7:329289/1‑149 (MQ=255)
                                                                                                  aTTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATg                                              <  8:414676/149‑1 (MQ=255)
                                                                                                      aTCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCAtt                                                                                                            <  8:102848/83‑1 (MQ=255)
                                                                                                      aTCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCAtt                                                                                                            >  7:102848/1‑83 (MQ=255)
                                                                                                       tCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTa                                         >  4:354368/1‑149 (MQ=255)
                                                                                                        cTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAAc                                                                              <  8:436205/111‑1 (MQ=255)
                                                                                                        cTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAAc                                                                              >  7:436205/1‑111 (MQ=255)
                                                                                                          gTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATg                                              >  4:257111/1‑141 (MQ=255)
                                                                                                          gTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATg                                              <  3:257111/141‑1 (MQ=255)
                                                                                                           tATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACgg                                      >  6:108923/1‑148 (MQ=255)
                                                                                                            aTTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGt                                     >  7:324616/1‑148 (MQ=255)
                                                                                                                     taAGAAATCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTcc                           <  6:343171/149‑1 (MQ=255)
                                                                                                                     taAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTc                            <  8:329289/148‑1 (MQ=255)
                                                                                                                       aGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCct                          >  4:366809/1‑148 (MQ=255)
                                                                                                                        gAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCctct                        >  1:247318/1‑149 (MQ=255)
                                                                                                                          aatcAACTTAATACCCGCACAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTACGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTcc                      <  6:313567/146‑1 (MQ=255)
                                                                                                                           aGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTACTCCCGCGCGTAATCCCAACGCTACCAACGTATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCg                     >  3:233061/1‑149 (MQ=255)
                                                                                                                             cAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTc                   >  2:3168/1‑149 (MQ=255)
                                                                                                                             cAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTc                   <  3:96313/149‑1 (MQ=255)
                                                                                                                              aaCTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTcc                  >  1:149868/1‑149 (MQ=255)
                                                                                                                                 ttAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTgc               >  1:244460/1‑149 (MQ=255)
                                                                                                                                 ttAATACCCGCAGAATGATTTCTGCGCGAAAGTATTAGCATATTATTTAGCTCATTAATCCCGCGCGTAATAACAACGCTACCAACGGATTAGGGAATAACACATACACTACATCATGGGTTACCCTAAAAAGATCCTCTCCGACCGgc               >  2:22449/1‑149 (MQ=255)
                                                                                                                                    ttACCCGCAGAAATCATTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCctct                        >  1:206850/2‑137 (MQ=255)
                                                                                                                                    ttACCCGCAGAAATCATTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCctct                        <  2:206850/136‑1 (MQ=255)
                                                                                                                                    ttACCCGCAGAAATCATTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTgcgc             >  4:273694/2‑148 (MQ=255)
                                                                                                                                    aTACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTgcgc             <  5:108923/148‑1 (MQ=255)
                                                                                                                                     tACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTa                                                                        <  3:294922/88‑1 (MQ=255)
                                                                                                                                     tACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTa                                                                        >  4:294922/1‑88 (MQ=255)
                                                                                                                                       cccGCAGAATGATTTCTTCGGGTAAGTATTAGCTTATTTTTTAGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAg          <  8:303821/148‑1 (MQ=255)
                                                                                                                                          gaaGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAAt      <  5:307185/147‑1 (MQ=255)
                                                                                                                                          gCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAAt      <  1:277811/149‑1 (MQ=255)
                                                                                                                                           cAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATg     <  3:62030/149‑1 (MQ=255)
                                                                                                                                              aaTGATTTCTGCGGGTAAGTATTAGCTGATTTTTTCGAGCATTAATCCCGCGCTTACTCCCAAAGCTACAAACTTATTAGGGAATAAAACATACTCTACATCATGGCTTACGGTAAAAAGTTCCTAACCGTCCTCCGCCAGCAATGTTc  >  1:120833/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GATGGAGAGTGATGAGAGAATATTACAACACGATGATTTTGCAGAGATTATGAAGAACTATACCGGATGACTGGTGATAAATAAAGCAAATAACCAGGATTAATCTGTATTAATTTATAAGAAAGCAACTTAATACCCGCAGAATGATTTCTGCGGGTAAGTATTAGCTTATTTTTTCGAGCATTAATCCCGCGCGTAATCCCAACGCTACCAACGGATTAGGGAATAACACATACTCTACATCATGGGTTACGGTAAAACGTTCCTCTCCGTCCTGCGCCAGCAATGTTC  >  NC_000913/1825792‑1826082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: