Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,352,970 T→A 6.7% I108I (ATT→ATA)  glpA → anaerobic sn‑glycerol‑3‑phosphate dehydrogenase, large FAD/NAD(P)‑binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,352,9700TA 6.7% 223.2 / 3.5 69I108I (ATT→ATA) glpAanaerobic sn‑glycerol‑3‑phosphate dehydrogenase, large FAD/NAD(P)‑binding subunit
Reads supporting (aligned to +/- strand):  ref base T (29/35);  new base A (3/2);  total (32/37)
Fisher's exact test for biased strand distribution p-value = 6.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGA  >  NC_000913/2352826‑2353106
                                                                                                                                                |                                                                                                                                        
tGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTcgcg                                                                                                                                      <  1:117770/149‑1 (MQ=255)
    gTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTg                                                                                                                                  <  2:374207/149‑1 (MQ=255)
        ccGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCgaa                                                                                                                              <  4:151695/149‑1 (MQ=255)
         cgatctggAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGGTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCGCTCCTTCCAGGCCACTTTTATTCGCGCCTGCgaag                                                                                                                             <  1:356560/143‑1 (MQ=255)
         cGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATACGCGCCTGCgaag                                                                                                                             <  4:399183/149‑1 (MQ=255)
         cGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATACGCGCCTGCg                                                                                                                                >  3:399183/1‑146 (MQ=255)
              cGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGc                                                                                                                         <  2:216417/148‑1 (MQ=255)
                gAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCgaac                                                                                                                             >  2:356560/1‑141 (MQ=255)
                         cgcgAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAgcg                                                                                                              >  3:94194/1‑148 (MQ=255)
                           cgAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAg                                                                                                           <  5:311248/149‑1 (MQ=255)
                                 cATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTTTCACTCTGCCGGAAGATGACCTCTCGTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCTGGGATGAGCGCAGAAGCt                                                                                                      <  2:124045/148‑1 (MQ=255)
                                 cATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCta                                                                                                     >  1:164420/1‑149 (MQ=255)
                                 cATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGAGGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCt                                                                                                      <  5:149984/148‑1 (MQ=255)
                                 cATTAGTGAAAACCAGATCCAGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTACTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCta                                                                                                     <  2:64314/149‑1 (MQ=255)
                                   ttAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTTTTTATCACCCTGCCGGAAGATGAACTCTCCTTCCAGGCCACTTTTATTCGCGCCTGAGAAGAAGCAGGTATAAGCGCAGAAGCtata                                                                                                   <  1:184049/149‑1 (MQ=255)
                                   ttAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCTCTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCta                                                                                                     >  5:203284/1‑147 (MQ=255)
                                   ttAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTCCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCtata                                                                                                   <  1:189966/149‑1 (MQ=255)
                                     aGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCta                                                                                                     <  1:84052/145‑1 (MQ=255)
                                     aGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCta                                                                                                     >  2:84052/1‑145 (MQ=255)
                                     aGTGAAAACCAGATCCTGAAACGCATTGCACGTAACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGa                                                                                                 >  8:392837/1‑149 (MQ=255)
                                      gTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGAc                                                                                                >  2:36252/1‑149 (MQ=255)
                                       tGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCCTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGAcc                                                                                               >  2:237154/1‑149 (MQ=255)
                                          aaaCCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCg                                                                                             >  7:192599/1‑148 (MQ=255)
                                            aCCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCgcag                                                                                          <  1:6240/149‑1 (MQ=255)
                                            aCCAGATCCTGAAACGCATTGCACGTCACTGCGATGAACCAACAAACGGCCTGTTTATCACCCTGAAGGAAGATCACCTATCCTACCGGGCCAATTTTTTACGCACATGCGAAGAACCTGGTATCAGCGCAGAAGCTTAAGAccag                                                                                             >  6:371390/1‑144 (MQ=255)
                                               aGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCaa                                                                                       <  7:217796/149‑1 (MQ=255)
                                                     tGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCACTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAg                                                                                                                >  1:193102/1‑118 (MQ=255)
                                                     tGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCACTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAg                                                                                                                <  2:193102/118‑1 (MQ=255)
                                                      gAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCa                                                                                >  5:322842/1‑149 (MQ=255)
                                                      gAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCa                                                                                >  7:278873/1‑149 (MQ=255)
                                                               tGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGaa                                                                        <  4:66950/148‑1 (MQ=255)
                                                                gCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAAcc                                                                      >  3:361793/1‑149 (MQ=255)
                                                                        cTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGtt                                                               >  5:245738/1‑148 (MQ=255)
                                                                             ttGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCg                                                         >  4:41997/1‑149 (MQ=255)
                                                                                aaccaaccAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCg                                                                          <  4:382069/129‑1 (MQ=255)
                                                                                aaccaaccAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCa                                                      <  8:192599/149‑1 (MQ=255)
                                                                                   caaccaacGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCt                                                                     <  8:337537/131‑1 (MQ=255)
                                                                                   caaccaacGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCt                                                                     >  7:337537/1‑131 (MQ=255)
                                                                                    aaccaacGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGa                                                  >  5:276293/1‑149 (MQ=255)
                                                                                       caacCGCCTGGTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTg                                               <  5:371390/149‑1 (MQ=255)
                                                                                         acGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGgc                                             <  6:322842/149‑1 (MQ=255)
                                                                                              cTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGt                                        >  6:131926/1‑149 (MQ=255)
                                                                                                 tttATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGa                                      <  2:164420/148‑1 (MQ=255)
                                                                                                 tttATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGa                                      >  7:59996/1‑148 (MQ=255)
                                                                                                       aCCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTcc                               >  8:365993/1‑149 (MQ=255)
                                                                                                          cTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGa                            <  8:278873/149‑1 (MQ=255)
                                                                                                           tGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGAt                           >  6:69247/1‑149 (MQ=255)
                                                                                                             ccGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGAt                           <  6:245738/147‑1 (MQ=255)
                                                                                                                        ccTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGAt               <  3:41997/148‑1 (MQ=255)
                                                                                                                        ccTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATc              >  5:46707/1‑149 (MQ=255)
                                                                                                                        ccTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATc              <  5:131926/149‑1 (MQ=255)
                                                                                                                              cTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTc        >  1:34155/1‑149 (MQ=255)
                                                                                                                               ttCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCg       >  5:414254/1‑149 (MQ=255)
                                                                                                                               ttCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCg       >  6:162802/1‑149 (MQ=255)
                                                                                                                                 ccAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGt      <  8:59996/148‑1 (MQ=255)
                                                                                                                                  cAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTc     <  6:414254/148‑1 (MQ=255)
                                                                                                                                  cAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTc     <  8:317400/148‑1 (MQ=255)
                                                                                                                                  cAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCt    >  1:410254/1‑149 (MQ=255)
                                                                                                                                   ttgaatcTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTg   <  5:25060/143‑1 (MQ=255)
                                                                                                                                   aGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGtt                                                               >  7:329700/1‑89 (MQ=255)
                                                                                                                                   aGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGtt                                                               <  8:329700/89‑1 (MQ=255)
                                                                                                                                    ggCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGa  <  4:94194/149‑1 (MQ=255)
                                                                                                                                    ggCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGa  <  8:109272/149‑1 (MQ=255)
                                                                                                                                        aCTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCgg                                                        <  1:297050/91‑1 (MQ=255)
                                                                                                                                        aCTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCgg                                                        >  2:297050/1‑91 (MQ=255)
                                                                                                                                          ttttATACGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGt      <  2:268743/139‑1 (MQ=255)
                                                                                                                                          ttttATACGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGt      >  1:268743/1‑139 (MQ=255)
                                                                                                                                           tttATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGAtt                                                >  3:5386/1‑96 (MQ=255)
                                                                                                                                           tttATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGAtt                                                <  4:5386/96‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                        
TGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCATTATCGAACCTGCCGTTAACCCGGCACTGATTGGCGCGGTGAAAGTTCCGGATGGCACCGTTGATCCATTTCGTCTGA  >  NC_000913/2352826‑2353106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑