Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,653,372 T→C 5.0% intergenic (+33/‑483) sseA → / → ryfA 3‑mercaptopyruvate sulfurtransferase/novel sRNA, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,653,3720TC 5.0% 314.0 / 5.4 100intergenic (+33/‑483)sseA/ryfA3‑mercaptopyruvate sulfurtransferase/novel sRNA, function unknown
Reads supporting (aligned to +/- strand):  ref base T (46/48);  new base C (2/3);  total (49/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.84e-01

Only 100 of 101 total aligned reads displayed.

CAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAATTATTGTTTATT  >  NC_000913/2653224‑2653516
                                                                                                                                                    |                                                                                                                                                
cAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAAttt                                                                                                                                                  >  1:206744/1‑149 (MQ=255)
 aGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCTTGGAGTGAATGGGGCGCGCGGGCAGATTTAGCGGTTGAGCCAGTGATATTAGTATTTTACAGGCAATAAAAAACCGCCGAATTTg                                                                                                                                                 <  5:385994/149‑1 (MQ=255)
   ccGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGc                                                                                                                                               >  3:339134/1‑149 (MQ=255)
    cGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCg                                                                                                                                              >  3:231614/1‑149 (MQ=255)
       ggTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGtt                                                                                                                                           <  4:25927/149‑1 (MQ=255)
        gTTTTGTTAGAACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGAGtttt                                                                                                                                          >  3:210199/1‑149 (MQ=255)
           ttGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGttttt                                                                                                                                        <  8:136872/148‑1 (MQ=255)
           ttGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGttttt                                                                                                                                        <  2:22871/148‑1 (MQ=255)
             gTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTAt                                                                                                                                     >  1:157455/1‑149 (MQ=255)
             gTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACAACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTa                                                                                                                                      <  3:386878/148‑1 (MQ=255)
              ttAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCAAATTCGGCGGTTTTTTAtt                                                                                                                                    <  4:42780/149‑1 (MQ=255)
                 gCACTCGCGACGCTGGATGTGCCAAACGGGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCt                                                                                                                                 <  4:289783/149‑1 (MQ=255)
                 ccccTCGCGACGCTGGATGAGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGCGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGc                                                                                                                                  <  5:340350/145‑1 (MQ=255)
                     tCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTGGGAGGTGTTTTATTGCTAGTc                                                                                                                             >  1:440115/1‑149 (MQ=255)
                         gACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGACTTTGGCGGGTTTGTATTGCTAGTCTGGt                                                                                                                         >  5:227546/1‑149 (MQ=255)
                           cGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTc                                                                                                                       >  4:149568/1‑149 (MQ=255)
                              tGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTcgcg                                                                                                                    >  8:219739/1‑149 (MQ=255)
                                gATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTa                                                                                                                                >  6:122133/1‑135 (MQ=255)
                                gATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTa                                                                                                                                <  5:122133/135‑1 (MQ=255)
                                  tgtgCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCt                                                                                                                >  3:218345/1‑149 (MQ=255)
                                     gCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTc                                                                                                             >  2:16959/1‑149 (MQ=255)
                                     gCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGGTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTc                                                                                                             <  5:38619/149‑1 (MQ=255)
                                      ccAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTcc                                                                                                            <  3:84322/149‑1 (MQ=255)
                                      ccAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTc                                                                                                             <  5:231664/148‑1 (MQ=255)
                                      ccAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTc                                                                                                             <  1:93913/148‑1 (MQ=255)
                                       cAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCca                                                                                                           >  2:24699/1‑149 (MQ=255)
                                        aaaCGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACACGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCcag                                                                                                          >  2:144674/1‑149 (MQ=255)
                                           cGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCcagcag                                                                                                       <  5:421046/149‑1 (MQ=255)
                                               aaaCTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGtt                                                                                                    <  4:272629/148‑1 (MQ=255)
                                                 aCTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGAc                                                                                                 <  4:339134/149‑1 (MQ=255)
                                                  cTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCAGGTTCGCGGCCTATCCAGCAGGTTGACt                                                                                                >  8:321512/1‑149 (MQ=255)
                                                            gcgcATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACAt                                                                                      <  7:219739/149‑1 (MQ=255)
                                                               cATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAg                                                                                   >  8:21463/1‑149 (MQ=255)
                                                                  ggAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCaa                                                                                >  7:39035/1‑149 (MQ=255)
                                                                  ggAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCaa                                                                                >  4:356479/1‑149 (MQ=255)
                                                                        aaTGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAgg                                                                          >  3:61724/1‑149 (MQ=255)
                                                                           ggggCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGc                                                                             <  6:395972/143‑1 (MQ=255)
                                                                           ggggCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGc                                                                             >  5:395972/1‑143 (MQ=255)
                                                                            gggCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGc                                                                       <  3:151288/148‑1 (MQ=255)
                                                                                 cgcgGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTcaca                                                                 <  6:329104/149‑1 (MQ=255)
                                                                                      gCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCaaa                                                            <  4:231614/149‑1 (MQ=255)
                                                                                        aGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACtt                                                                                               <  7:219031/112‑1 (MQ=255)
                                                                                        aGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACtt                                                                                               >  8:219031/1‑112 (MQ=255)
                                                                                        aGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAAc                                                          <  4:210789/149‑1 (MQ=255)
                                                                                          aTTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACa                                                         >  2:366317/1‑148 (MQ=255)
                                                                                          aTTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTAGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACaa                                                        >  7:36077/1‑149 (MQ=255)
                                                                                           tttACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGtt                                                                                          <  3:219885/114‑1 (MQ=255)
                                                                                           tttACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGtt                                                                                          >  4:219885/1‑114 (MQ=255)
                                                                                             tACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATa                                                      <  3:356479/148‑1 (MQ=255)
                                                                                                cGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCt                                                                                                                >  4:369523/1‑87 (MQ=255)
                                                                                                cGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCt                                                                                                                <  3:369523/87‑1 (MQ=255)
                                                                                                 ggTTGAGCCAGTGAAATAATTATTTTACAGGCAATAAAAAACCGCCGAATTTTGCGGTTTTTTATTGCTAGTCTTGTTCGCGTCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCAc                                                 <  2:361545/149‑1 (MQ=255)
                                                                                                 ggTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGACTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCAc                                                 <  1:3742/149‑1 (MQ=255)
                                                                                                  gTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCAc                                                 >  6:364094/1‑148 (MQ=255)
                                                                                                   ttGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCAc                                                 >  3:306961/1‑147 (MQ=255)
                                                                                                      aGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGt                                             >  5:89361/1‑148 (MQ=255)
                                                                                                      aGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGt                                             >  7:259692/1‑148 (MQ=255)
                                                                                                       gCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTa                                            >  4:218544/1‑148 (MQ=255)
                                                                                                        ccAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAAc                                          >  8:249300/1‑149 (MQ=255)
                                                                                                         cAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAAct                                         >  2:50395/1‑149 (MQ=255)
                                                                                                          aGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAActc                                        >  2:322475/1‑149 (MQ=255)
                                                                                                              aaaTAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCATTAACTCTCtt                                    <  1:24699/149‑1 (MQ=255)
                                                                                                              aaaTAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCtt                                    >  1:211287/1‑149 (MQ=255)
                                                                                                                aTAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCtttt                                  >  1:176397/1‑149 (MQ=255)
                                                                                                                 tAAGTATTTTACAGGCAATAAAAACCCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGGTCGCGGCCTTTCCAGCAGGTTGACTTGTGTGACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCttttt                                 <  4:210199/149‑1 (MQ=255)
                                                                                                                 tAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCttttt                                 <  1:144674/149‑1 (MQ=255)
                                                                                                                 tAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCttttt                                 <  7:21463/149‑1 (MQ=255)
                                                                                                                 tAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCttttt                                 >  2:93936/1‑149 (MQ=255)
                                                                                                                  aaGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCCGTAACTCTCTTTTTg                                >  6:260130/1‑149 (MQ=255)
                                                                                                                   aGTATTTTACAGGCAATAAAAACCCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGt                               <  1:189144/149‑1 (MQ=255)
                                                                                                                    gTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGt                               >  4:284640/1‑148 (MQ=255)
                                                                                                                       ttttACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCaa                            <  1:93936/148‑1 (MQ=255)
                                                                                                                       ttttACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTGGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAg                           <  5:236422/149‑1 (MQ=255)
                                                                                                                         ttACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGc                          <  8:259692/148‑1 (MQ=255)
                                                                                                                         ttACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGc                          >  6:246937/1‑148 (MQ=255)
                                                                                                                            cAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCaaaa                      <  6:68683/149‑1 (MQ=255)
                                                                                                                             aGGCAATAAAAATCCGCCGAATTTGTCTGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTAGTGTTACATGATCAACGCAGGTGCTTCACAGCAAAACAATAATCACCAGTAACTCTCTTTTTGTCAAGCAAAag                     <  8:36077/149‑1 (MQ=38)
                                                                                                                             aGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCGGTAACTCTCTTTTTGTCAAGCaaaa                      <  1:366317/148‑1 (MQ=255)
                                                                                                                              ggCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAaga                    <  5:364094/149‑1 (MQ=255)
                                                                                                                               gCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCATAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCaaaa                      >  5:402221/1‑146 (MQ=255)
                                                                                                                                 aaTAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAgg                                                                          >  8:3607/1‑92 (MQ=255)
                                                                                                                                 aaTAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAgg                                                                          <  7:3607/92‑1 (MQ=255)
                                                                                                                                 aaTAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCaaa                                                            >  1:426311/1‑106 (MQ=255)
                                                                                                                                  aTAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGGGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGt                <  2:440115/149‑1 (MQ=255)
                                                                                                                                  aTAAAAAACCGCCAAATTCGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCttttt                                 <  6:163153/132‑1 (MQ=255)
                                                                                                                                  aTAAAAAACCGCCAAATTCGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCttttt                                 >  5:163153/1‑132 (MQ=255)
                                                                                                                                   tAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGt                >  2:269851/1‑148 (MQ=255)
                                                                                                                                   tAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCCGCCTTTCAAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCAAAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGt                >  1:113856/1‑148 (MQ=255)
                                                                                                                                   tAAAAAACCGCCAAATTCGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAg                                                                           >  5:195570/1‑89 (MQ=255)
                                                                                                                                   tAAAAAACCGCCAAATTCGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAg                                                                           <  6:195570/89‑1 (MQ=255)
                                                                                                                                    aaaaaaCCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCAc                                                 >  4:309971/1‑114 (MQ=255)
                                                                                                                                    aaaaaaCCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCAc                                                 <  3:309971/114‑1 (MQ=255)
                                                                                                                                        aaCCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAattat          <  3:149568/149‑1 (MQ=255)
                                                                                                                                         aCCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAattatt         <  1:322475/149‑1 (MQ=255)
                                                                                                                                          ccgccgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCATAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAATTATTg        <  6:402221/149‑1 (MQ=255)
                                                                                                                                            gccgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCTGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAATTATTGtt      <  3:281527/149‑1 (MQ=255)
                                                                                                                                            gccgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAATTATTGtt      >  5:231582/1‑149 (MQ=255)
                                                                                                                                             ccgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAATTATTGtt      >  2:28099/1‑148 (MQ=255)
                                                                                                                                              cgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAATTATTGttta    >  8:168207/1‑149 (MQ=255)
                                                                                                                                                aaTTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAATTATTGtttatt  <  2:211287/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACCAGTAACTCTCTTTTTGTCAAGCAAAAGAGAGTAATTATTGTTTATT  >  NC_000913/2653224‑2653516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: