Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,806,319 C→T intergenic (‑236/‑51) ydiY ← / → pfkB acid‑inducible putative outer membrane protein/6‑phosphofructokinase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,806,3190CT97.1% 238.5 / NA 71intergenic (‑236/‑51)ydiY/pfkBacid‑inducible putative outer membrane protein/6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (33/35);  minor base A (2/0);  total (36/35)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTAC  >  NC_000913/1806180‑1806461
                                                                                                                                           |                                                                                                                                              
ttctggtctgacggcgcttactgctgaattcactgtcggcgaatgtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCg                                                                                                                                        >  1:336623‑M1/130‑148 (MQ=255)
 tctggtctgacggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGc                                                                                                                                       >  3:399263‑M1/129‑148 (MQ=255)
     gtctgacggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGAtt                                                                                                                                  <  4:28910‑M1/25‑1 (MQ=255)
      tctgacggcgattactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTc                                                                                                                                 >  7:327497‑M1/124‑149 (MQ=255)
          acggcgcttactgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGt                                                                                                                              >  1:55994‑M1/120‑148 (MQ=255)
                   actgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTg                                                                                                                     <  8:287553‑M1/38‑1 (MQ=255)
                    ctgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGaa                                                                                                                   >  7:151523‑M1/110‑149 (MQ=255)
                     tgctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGaaa                                                                                                                  <  7:272661‑M1/41‑1 (MQ=255)
                       ctgaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctggtctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTTATTCGGTGCCAGACTGAAAt                                                                                                                 <  1:259880‑M1/42‑1 (MQ=255)
                         gaattcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTCCTTTCCGCTGATTCGGTGCCAGACTGAAATCAg                                                                                                              <  4:205033‑M1/45‑1 (MQ=255)
                           attcactgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatagcagggacttgttcgcacgttccTTAATTTCTTACTTTCCGCTGATTCGGTGTCAGACTGAAATCAGcc                                                                                                            >  4:287635‑M1/103‑148 (MQ=255)
                               actgtcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCta                                                                                                          <  7:143514‑M1/49‑1 (MQ=255)
                                   tcggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATagga                                                                                                     >  3:28910‑M1/95‑148 (MQ=255)
                                     ggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATaggagg                                                                                                   >  7:137618‑M1/93‑148 (MQ=255)
                                     ggcgaaggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATaggagg                                                                                                   >  4:209215‑M1/93‑148 (MQ=255)
                                          aggtaagttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAatga                                                                                             <  1:154323‑M1/62‑1 (MQ=255)
                                                gttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGaaa                                                                                                                  >  6:74884‑M1/82‑122 (MQ=255)
                                                gttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGaaa                                                                                                                  <  5:74884‑M1/41‑1 (MQ=255)
                                                gttgatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTAAATTTCTTAACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTAc                                                                                       >  6:64784‑M1/82‑149 (MQ=255)
                                                   gatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGt                                                                                                                              >  4:36236‑M1/79‑107 (MQ=255)
                                                   gatgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGt                                                                                                                              <  3:36236‑M1/29‑1 (MQ=255)
                                                     tgactcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATc                                                                                  >  8:4889‑M1/77‑149 (MQ=255)
                                                       actcatgatgaaccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCt                                                                                 >  8:272661‑M1/75‑148 (MQ=255)
                                                                   ccctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGACTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACt                                                                    <  2:329085‑M1/87‑1 (MQ=255)
                                                                    cctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCa                                                                                                               <  8:38096‑M1/44‑1 (MQ=255)
                                                                    cctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACtt                                                                   <  1:25357‑M1/88‑1 (MQ=255)
                                                                    cctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACtt                                                                   <  2:376175‑M1/88‑1 (MQ=255)
                                                                    cctgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACtt                                                                   >  7:287553‑M1/62‑149 (MQ=255)
                                                                      tgttctatggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTgc                                                                 <  1:23669‑M1/90‑1 (MQ=255)
                                                                              ggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGacac                                                                     <  6:149086‑M1/86‑1 (MQ=255)
                                                                              ggctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGacac                                                                     >  5:149086‑M1/52‑137 (MQ=255)
                                                                               gctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCctctc                                                         >  1:225361‑M1/51‑148 (MQ=255)
                                                                                ctccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCtctctc                                                       >  3:205033‑M1/50‑149 (MQ=255)
                                                                                  ccagatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGa                                                     <  5:184646‑M1/102‑1 (MQ=255)
                                                                                    agatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGa                                                     <  8:15891‑M1/102‑1 (MQ=255)
                                                                                    agatgacaaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATa                                                   >  6:184646‑M1/46‑149 (MQ=255)
                                                                                      atgacaaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATa                                                   >  3:58164‑M1/44‑147 (MQ=255)
                                                                                      atgacaaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATa                                                   <  4:58164‑M1/104‑1 (MQ=255)
                                                                                          caaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGtt                                                                          >  5:354917‑M1/40‑120 (MQ=255)
                                                                                          caaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGtt                                                                          >  5:348872‑M1/40‑120 (MQ=255)
                                                                                          caaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGtt                                                                          <  6:354917‑M1/81‑1 (MQ=255)
                                                                                          caaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGtt                                                                          <  6:348872‑M1/81‑1 (MQ=255)
                                                                                           aaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTGCTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGcaa                                             <  7:334869‑M1/110‑1 (MQ=255)
                                                                                           aaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAatgat                                                                                            >  6:44267‑M1/39‑101 (MQ=255)
                                                                                           aaacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGcaa                                             <  8:340003‑M1/110‑1 (MQ=255)
                                                                                            aacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCtctctc                                                       >  1:113221‑M1/38‑137 (MQ=255)
                                                                                            aacatgatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCtctctc                                                       <  2:113221‑M1/100‑1 (MQ=255)
                                                                                                 gatctcatatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTAc                                      <  5:203840‑M1/117‑1 (MQ=255)
                                                                                                       atatcagggagttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATTGGAGGAAATGATGGTACGTATCTATACGTTGACACTTgc                                                                 >  7:19456‑M1/27‑116 (MQ=255)
                                                                                                       atatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGc                                 <  7:208276‑M1/122‑1 (MQ=255)
                                                                                                       atatcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATCATGGTACGTATCAATACGTTGACACTTgc                                                                 <  8:19456‑M1/90‑1 (MQ=255)
                                                                                                          tcagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACATTTACCCCGCAAAt                             >  1:376175‑M1/24‑149 (MQ=255)
                                                                                                           cagggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAAtt                            <  7:392219‑M1/127‑1 (MQ=255)
                                                                                                            agggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGaaa                                                                                                                  <  8:229926‑M1/41‑1 (MQ=255)
                                                                                                            agggacttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGaaa                                                                                                                  >  7:229926‑M1/22‑62 (MQ=255)
                                                                                                              ggccttgttcgcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGGGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGAAACAATTACCCCGCAAATTTAt                         <  5:74511‑M1/130‑1 (MQ=255)
                                                                                                              catcacctAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTAt                         <  3:23841/142‑1 (MQ=255)
                                                                                                                        gcaccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGaa               >  2:25357‑M1/10‑149 (MQ=255)
                                                                                                                           ccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAAc            <  6:415176‑M1/143‑1 (MQ=255)
                                                                                                                           ccttccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGAAAATTTATCCCGAaggaacac            <  6:367139‑M1/143‑4 (MQ=255)
                                                                                                                            cttccTTAATTTCTTTACTTTCCACTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACt           <  2:320742‑M1/144‑1 (MQ=255)
                                                                                                                               ccTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTg          >  2:23669‑M1/3‑147 (MQ=255)
                                                                                                                                 ttAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCt      >  3:107239/1‑149 (MQ=255)
                                                                                                                                 ttAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCt      >  6:399942/1‑149 (MQ=255)
                                                                                                                                 ttAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCt      >  2:354761/1‑149 (MQ=255)
                                                                                                                                 ttAAATTCTTAACATTCCGCTGATTCGGTGCCAGACACAAATCAGCCTATAGGAGGAAAACATGTTACCTAAATATACGTTGACACTACCGCACACTCTAGATAGCGCAACAATTACCCCCCAAATTTAACCAGAAGGAAAACttct        >  4:342791/1‑144 (MQ=255)
                                                                                                                                  tAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTg     >  5:20703/1‑149 (MQ=255)
                                                                                                                                  tAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTg     >  5:258565/1‑149 (MQ=255)
                                                                                                                                    aTTTCTTTGCTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGt    <  8:334869/148‑1 (MQ=255)
                                                                                                                                    aTTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTa   >  3:315432/1‑149 (MQ=255)
                                                                                                                                     tttCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTAc  <  8:143514/149‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                              
TAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTAC  >  NC_000913/1806180‑1806461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: