Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,807,226 C→G A286G (GCC→GGC)  pfkB → 6‑phosphofructokinase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,807,2260CG92.3% 303.1 / 12.2 91A286G (GCC→GGC) pfkB6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base C (3/4);  new base G (40/44);  total (43/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTC  >  NC_000913/1807081‑1807369
                                                                                                                                                 |                                                                                                                                               
tGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGCCACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGca                                                                                                                                               >  6:65157/1‑148 (MQ=255)
tGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGca                                                                                                                                               >  6:309300/1‑148 (MQ=255)
    ttCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTc                                                                                                                                          <  5:127514/149‑1 (MQ=255)
     tCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGTCAGAAAATGCCTCTCTTGAAGAGATGGGTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTc                                                                                                                                          >  3:254070/1‑148 (MQ=255)
       aGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGCGCAGGCACACTCaa                                                                                                                                        >  7:380866/1‑148 (MQ=255)
       aGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGCGCAGGCACACTCAAt                                                                                                                                       >  7:374713/1‑149 (MQ=255)
         gtggtgCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAAt                                                                                                                                       >  7:233002/1‑147 (MQ=255)
          tggtgCCACCAGCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTAGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCa                                                                                                                                     <  5:378362/148‑1 (MQ=255)
           ggtgCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAgg                                                                                                                                   <  6:293943/149‑1 (MQ=255)
            gtgCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAggg                                                                                                                                  <  4:393267/149‑1 (MQ=255)
            gtgCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAgg                                                                                                                                   <  3:250586/148‑1 (MQ=255)
            gtgCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAgg                                                                                                                                   <  7:63063/148‑1 (MQ=255)
            gtgCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCGGCGGGGAGTGCAGGCACACTCAATCAgg                                                                                                                                   <  8:381114/148‑1 (MQ=255)
               ccaccaCCGGTGAAAAGCCGGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGaa                                                                                                                                >  6:305321/1‑148 (MQ=255)
               ccaccaCCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTTCAGGCACACTCAATCAGGGAac                                                                                                                               >  5:289901/1‑149 (MQ=255)
               ccaccaCCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAac                                                                                                                               <  7:175696/149‑1 (MQ=255)
                caccacCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGaa                                                                                                                                <  7:394072/147‑1 (MQ=255)
                caccacCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGaa                                                                                                                                >  8:394072/1‑147 (MQ=255)
                 accaccGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACATCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAacac                                                                                                                             >  2:856/1‑149 (MQ=255)
                    accGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGcaca                                                                                                                                             >  2:369978/1‑130 (MQ=255)
                    accGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGcaca                                                                                                                                             <  1:369978/130‑1 (MQ=255)
                     ccGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCt                                                                                                                         <  1:289131/149‑1 (MQ=255)
                       ggTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCtgt                                                                                                                       >  1:400642/1‑149 (MQ=255)
                       agTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCtgt                                                                                                                       <  2:148469/148‑1 (MQ=255)
                         tGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGc                                                                                                                     <  8:414528/149‑1 (MQ=255)
                                acAGAGTACCGTTCGCGCTGGTGACAGCATGGTAGGCGAGATGAAACTGAAACAGGCAGAAAATGCCTCTCTTGAAGAGATGCTTCGTATAGGCGTAGCTGCGGGGAGTGCAGGCACACTCAAACAGGCAACACGTCTGTGCTcaaat                                                                                                               >  4:391593/2‑144 (MQ=255)
                                 cAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTAGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGa                                                                                                             >  4:254967/1‑149 (MQ=255)
                                   gagTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACg                                                                                                           >  3:256755/1‑149 (MQ=255)
                                     gTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGAt                                                                                                         <  1:101961/149‑1 (MQ=255)
                                        ccGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATAc                                                                                                       <  3:81325/148‑1 (MQ=255)
                                        ccGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCGGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACg                                                                                                      <  8:233002/149‑1 (MQ=255)
                                                gcTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCACTCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAAt                                                                                              >  2:98144/1‑149 (MQ=255)
                                                    gTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTa                                                                                           <  8:161842/148‑1 (MQ=255)
                                                    gTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTAc                                                                                          >  3:367371/1‑149 (MQ=255)
                                                      gACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGc                                                                                        >  7:269865/1‑149 (MQ=255)
                                                         aGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGAAACGCAAAAAATTTACGCTTa                                                                                     >  2:360512/1‑149 (MQ=255)
                                                           cATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTAcc                                                                                   >  8:59926/1‑149 (MQ=255)
                                                                tCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTcc                                                                              >  4:207155/1‑149 (MQ=255)
                                                                tCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTcc                                                                              >  7:12078/1‑149 (MQ=255)
                                                                  ggCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCg                                                                            <  8:156171/149‑1 (MQ=255)
                                                                     gcgATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCt                                                                          >  5:132726/1‑148 (MQ=255)
                                                                     gcgATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCt                                                                          >  4:113666/1‑148 (MQ=255)
                                                                     gcgATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCt                                                                          >  4:163329/1‑148 (MQ=255)
                                                                      cgATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTaa                                                                        <  3:207155/149‑1 (MQ=255)
                                                                         tGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACa                                                                      >  4:334304/1‑148 (MQ=255)
                                                                          gACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACaa                                                                     <  3:254967/148‑1 (MQ=255)
                                                                            cacTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACaaaaa                                                                  <  1:253211/149‑1 (MQ=255)
                                                                            cacTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACaaaaa                                                                  <  2:400642/149‑1 (MQ=255)
                                                                             acTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACaaaaa                                                                  >  8:354771/1‑148 (MQ=255)
                                                                               tGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAAttt                                                                                            <  5:337722/120‑1 (MQ=255)
                                                                               tGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAAttt                                                                                            >  6:337722/1‑120 (MQ=255)
                                                                               tGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACAt                                                               >  2:215186/1‑149 (MQ=255)
                                                                               tGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACAt                                                               >  4:75430/1‑149 (MQ=255)
                                                                                         aGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCa                                                     >  2:253539/1‑149 (MQ=255)
                                                                                            aaaTGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTg                                                  <  6:132726/149‑1 (MQ=255)
                                                                                             aaTGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGATGCGGGGAGTGCCGGCACACTAAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCGAACAAAAACATTCCCCCAGCAGTgg                                                 >  5:103851/1‑149 (MQ=255)
                                                                                              aTGCCTCTCTTGATGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTggg                                                <  4:49150/149‑1 (MQ=255)
                                                                                              aTGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTggg                                                <  3:113666/149‑1 (MQ=255)
                                                                                              aTGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTggg                                                <  3:163329/149‑1 (MQ=255)
                                                                                              aTGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGCGCAGGCACACTCAATCAGGTAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTggg                                                <  8:374713/149‑1 (MQ=255)
                                                                                              aTGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGCGCAGGCACACTCAATCAGGGAACACGTCTGTGCTACCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTggg                                                <  8:380866/149‑1 (MQ=255)
                                                                                               tGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTgggg                                               <  4:256755/149‑1 (MQ=255)
                                                                                               tGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTgggg                                               >  3:292818/1‑149 (MQ=255)
                                                                                                  ctctctTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCa                                                        <  4:75461/137‑1 (MQ=255)
                                                                                                  ctctctTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCa                                                        >  3:75461/1‑137 (MQ=255)
                                                                                                  ctctctTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGaa                                            >  1:216211/1‑149 (MQ=255)
                                                                                                     tctTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAatca                                         >  7:206143/1‑149 (MQ=255)
                                                                                                     tctTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAATGCTGGGGGAat                                           <  7:341954/147‑1 (MQ=255)
                                                                                                     tctTGAAGAGATGGATCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAat                                           <  3:391593/147‑1 (MQ=255)
                                                                                                       ttGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAatc                                          <  3:75430/146‑1 (MQ=255)
                                                                                                       ttGAAGAGATCGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATCCGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAatc                                          <  4:209662/146‑1 (MQ=255)
                                                                                                         gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAatcatc                                       <  5:309300/147‑1 (MQ=255)
                                                                                                         gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAat                                           <  7:72583/143‑1 (MQ=255)
                                                                                                         gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAat                                           >  8:72583/1‑143 (MQ=255)
                                                                                                         gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAAtcatca                                      <  1:408049/148‑1 (MQ=255)
                                                                                                         gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAAtcatca                                      <  1:856/148‑1 (MQ=255)
                                                                                                            gagaTGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCaa                                  <  6:289901/149‑1 (MQ=255)
                                                                                                             agaTGGGTCGGTTTGGCGTAGCTGCGGGGAGGGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAAc                                 <  3:205599/149‑1 (MQ=255)
                                                                                                                 ggTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTg                              <  4:292818/148‑1 (MQ=255)
                                                                                                                  gTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTc                            >  3:193504/1‑149 (MQ=255)
                                                                                                                  gTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTc                            >  4:149133/1‑149 (MQ=255)
                                                                                                                     cGTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCgg                          <  3:334304/148‑1 (MQ=255)
                                                                                                                      gTTTTGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGc                         <  4:254070/148‑1 (MQ=255)
                                                                                                                       ttttGGCGTAGCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCaa                       >  1:259791/1‑149 (MQ=255)
                                                                                                                                 gCTGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTtctc             <  2:259791/149‑1 (MQ=255)
                                                                                                                                   tGCGGGGAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCAAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTcc            >  6:82529/1‑148 (MQ=255)
                                                                                                                                     cGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGa          <  3:149133/148‑1 (MQ=255)
                                                                                                                                       gggAGTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTa       >  1:217132/1‑149 (MQ=255)
                                                                                                                                          tgTGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATTAGCAAAAAATTTACGCTTACCTTTCCCGCTAACTAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATga    <  6:103851/148‑2 (MQ=255)
                                                                                                                                           gTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCt   >  3:207300/1‑149 (MQ=255)
                                                                                                                                            tGCAGGCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTc  <  7:354771/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTC  >  NC_000913/1807081‑1807369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑