| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,840,946 | 0 | C | . | 22.2% | 42.4 / 10.1 | 18 | coding (458/621 nt) | ynjF | CDP‑alcohol phosphatidyltransferase family inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (6/8); new base . (2/2); total (8/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.81e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CACTCCACACCCGCGTAAAGGTCGTCATCCAGCACAACGCACCGAAGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACATCCCAGCACAAACAGTAAGATCGTTTCTGTGCCTTCGGTTAATCCCCCCAGATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTG > NC_000913/1840818‑1841085 | cACTCCACACCCGCGTAAAGGTCGTCATCCAGCACAACGCACCGAAGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACATCCCAGCACAAACAGTAAGATCGTTTCTGTGCCTTCGGTTAATCCCCCCAGATAGTAAAACGAc < 6:164089/149‑1 (MQ=255) acCCGCGTAAAGGTCGTCATCCAGCACAACGCACCGAAGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACATCCCAGCACAAACAGTAAGATCGTTTCTGTGCCTTCGGTTAAT‑CCCCCAGATAGTAAAAc < 5:1579/137‑1 (MQ=255) acCCGCGTAAAGGTCGTCATCCAGCACAACGCACCGAAGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACATCCCAGCACAAACAGTAAGATCGTTTCTGTGCCTTCGGTTAAT‑CCCCCAGATAGTAAAAc > 6:1579/1‑137 (MQ=255) aTCCAGCACAACGCACCGAAGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACATCCCAGCACAAACAGTAAGATCGTTTCTGTGCCTTCGGTTAATCCCCCCAGATAGTAAAACGACTTATGTGCATAACCg < 5:114470/138‑1 (MQ=255) aTCCAGCACAACGCACCGAAGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACATCCCAGCACAAACAGTAAGATCGTTTCTGTGCCTTCGGTTAATCCCCCCAGATAGTAAAACGACTTATGTGCATAACCg > 6:114470/1‑138 (MQ=255) gCACAACGCACCGAAGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACATCCCAGCACAAACAGTAAGATCGTTTCTGTGCCTTCGGTTAATCCCCCCAGa < 3:35145/106‑1 (MQ=255) gCACAACGCACCGAAGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACATCCCAGCACAAACAGTAAGATCGTTTCTGTGCCTTCGGTTAATCCCCCCAGa > 4:35145/1‑106 (MQ=255) aCCGAAGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACATCCCAGCACAAACAGTAAGATCGTTTCTGTGCCTTCGGTTAATCCCCCCAGATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTtgctg > 5:92652/1‑149 (MQ=255) gAAGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACATCCCAGCACAAACAGTAAGATCGTTTCTGTGCCTTCGGTTAAT‑CCCCCAGATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAg > 5:164297/1‑149 (MQ=255) atccacgcaatccacgcaAACCATGCAGGGAATAAACATCCCAGCACAAACAGTAAGATCGTTTCTGTGCCTTCGGTTAATCCCCCCa < 2:30876/88‑1 (MQ=255) cccAGCACAAACAGTAAGATCGTTTCTGTGCCTTCGGTTAATCCCCCCAGATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAActg < 6:82452/129‑1 (MQ=255) cccAGCACAAACAGTAAGATCGTTTCTGTGCCTTCGGTTAATCCCCCCAGATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAActg > 5:82452/1‑129 (MQ=255) caAACAGTAAGATCGTTTCTGTGCCTTCGGTTAATCCCCCCAGATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAaacaa > 2:46848/1‑148 (MQ=255) gTTTCTGTGCCTTCGGTTAATCCCCCCAGATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGc < 6:92652/149‑1 (MQ=255) tttCTGTGCCTTCGGTTAAT‑CCCCCAGATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCa < 6:164297/149‑1 (MQ=255) cGGTTAATCCCCCCAGATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTg < 1:46848/148‑1 (MQ=255) aTCCCCCCAGATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTtgct > 2:30395/1‑62 (MQ=255) aTCCCCCCAGATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTtgct < 1:30395/62‑1 (MQ=255) | CACTCCACACCCGCGTAAAGGTCGTCATCCAGCACAACGCACCGAAGATCCACGCAAACCACGCAAACCATGCAGGGAATAAACATCCCAGCACAAACAGTAAGATCGTTTCTGTGCCTTCGGTTAATCCCCCCAGATAGTAAAACGACTTATGTGCATAACCGGGGTTATCAATCTGATGTTTTGCTGCCAGTGCAGCAAAGGCGAGAAAACTGCTGCCCGTGCCGATAAACGCAAACAACAACCAGCCGCCTGCCAGCGCATTTTG > NC_000913/1840818‑1841085 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |