Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,418,509 Δ1 bp 5.6% coding (1429/1521 nt) yfcC → putative inner membrane transporter; C4‑dicarboxylate anaerobic carrier family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,418,5090A. 5.6% 230.2 / 6.1 72coding (1429/1521 nt)yfcCputative inner membrane transporter; C4‑dicarboxylate anaerobic carrier family protein
Reads supporting (aligned to +/- strand):  ref base A (34/34);  new base . (2/2);  total (36/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

ACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTAC  >  NC_000913/2418364‑2418657
                                                                                                                                                 |                                                                                                                                                    
aCTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAAc                                                                                                                                                    <  2:234191/148‑1 (MQ=255)
aCTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAAc                                                                                                                                                    <  7:225481/148‑1 (MQ=255)
    gCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCt                                                                                                                                               >  8:37497/1‑149 (MQ=255)
     cACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCt                                                                                                                                               >  6:184233/1‑148 (MQ=255)
          cTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCTTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCTGTACTGGCTGAAGGTg                                                                                                                                        >  8:237305/1‑149 (MQ=255)
          cTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCTGTACTGGCTGAAGGTg                                                                                                                                        >  8:243046/1‑149 (MQ=255)
           ttGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTg                                                                                                                                        >  1:108254/1‑149 (MQ=255)
                      gTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGAccc                                                                                                                              <  2:171015/148‑1 (MQ=255)
                      gTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGAccc                                                                                                                              >  7:355358/1‑148 (MQ=255)
                             ttAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGa                                                                                                                      <  3:164379/149‑1 (MQ=255)
                                 ccGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGCCCCTACTTGGActgc                                                                                                                  >  6:170537/1‑149 (MQ=255)
                                  cGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGActgct                                                                                                                 <  8:351695/149‑1 (MQ=255)
                                    tCAGGTTACCGTGCTGGTGTTACAGTTTGGTGATGGCTTCAGTCACATAATTTACACAACCTCAGCTTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGCCTTCCGTAACTGGCTGAAGGTGCGCGCGACCCTACTTGGActgcatt                                                                                                               >  6:197084/1‑146 (MQ=255)
                                        gTTTCCGTGCTGGCGTTCGAGTTTGGTGATGGCTTCAGTCACTTCATTTAGCCAACCTCTGCGTCGTTAATGGAAACTCTCGTTGTGTGCAGGGTGGACTTCCGTAACTGGCTGATGGTGGGCGCGACGCTACTTGGTCTGGTGTttat                                                                                                           <  6:253479/149‑1 (MQ=255)
                                        gTTACCGTGCTGGCGTTCCAGTTTGGTGCTGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGGGTGTGCAGGGGGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTttat                                                                                                           <  7:306014/149‑1 (MQ=255)
                                           aCCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTttatta                                                                                                         <  6:15270/148‑1 (MQ=255)
                                           aCCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTttatta                                                                                                         <  7:37497/148‑1 (MQ=255)
                                           aCCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTtattat                                                                                                        >  1:78874/1‑149 (MQ=255)
                                                    gCGTTCCAGTGTGGTGCTGGCCTCACTCACATCATTTACCCAACCTCCGCGGCGTTACTGGCACCGCTCGGTGTGGGCAGGGTGGACTTCCGTAACTGGCTGCAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGcg                                                                                                <  6:52518/148‑1 (MQ=255)
                                                      gTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGcgtcg                                                                                             >  5:44343/1‑149 (MQ=255)
                                                      gTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGcgtcg                                                                                             >  7:287811/1‑149 (MQ=255)
                                                         ccAGTTTGGTGATGGATTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTa                                                                                           <  6:185978/148‑1 (MQ=255)
                                                          cAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGt                                                                                         >  1:63107/1‑149 (MQ=255)
                                                                ggTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCgg                                                                                   >  1:175886/1‑149 (MQ=255)
                                                                ggTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCg                                                                                    <  5:184233/148‑1 (MQ=255)
                                                                 gTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTGTTATGTCCAGCGTCGTAGTGATCGgc                                                                                  >  4:314938/1‑149 (MQ=255)
                                                                   gATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGgcgc                                                                                >  1:239707/1‑149 (MQ=255)
                                                                     tGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTc                                                                              <  2:239707/149‑1 (MQ=255)
                                                                       gCTTCAGTCTCATCATTTACCCAACCTCAGCGTAGTTAATGTCAACGCTCGGTGGGGTCAGTGTGGACTTCCGTAACTGGCTGAATGTGGTAGCGACCCTACTTGGAATGCTGTTTATTATGTCGAGCGTCGTAGTGTTCGGCGCTCa                                                                             <  2:237717/148‑1 (MQ=255)
                                                                           cAGTGACATCATTTACCCAACCTCAGCGGCGTTTATGGCAACGCTCTGTGTGTGCAGGGTTGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGtt                                                                          <  5:197084/147‑1 (MQ=255)
                                                                            aGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGAt                                                                       <  8:355358/149‑1 (MQ=255)
                                                                            aGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTGATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGAt                                                                       >  6:284554/1‑149 (MQ=255)
                                                                              tCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATgg                                                                     >  4:121613/1‑149 (MQ=255)
                                                                                 catcatTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCt                                                                  <  8:287811/149‑1 (MQ=255)
                                                                                  atcatTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCTGTACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTAc                                                                <  7:243046/149‑1 (MQ=255)
                                                                                  atcatTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCTGTACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTAc                                                                <  7:237305/149‑1 (MQ=255)
                                                                                  atcatTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTa                                                                 >  6:327928/1‑149 (MQ=255)
                                                                                   tcatTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTGTTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTAc                                                                <  3:314938/149‑1 (MQ=255)
                                                                                   tcatTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTAc                                                                >  5:240244/1‑149 (MQ=255)
                                                                                   tcatTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTAc                                                                >  5:141554/1‑149 (MQ=255)
                                                                                   tcatTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTAc                                                                >  4:234375/1‑149 (MQ=255)
                                                                                   tcatTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTAc                                                                <  2:78874/149‑1 (MQ=255)
                                                                                   tcatTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGCGTGTGCAGGGGGGACTGCAGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCAAGCGTCGTAGTGATCGGCGCTCAGTTTATGGGCTAc                                                                <  6:338450/149‑1 (MQ=255)
                                                                                         aCCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTa                                                           >  6:71266/1‑148 (MQ=255)
                                                                                         aCCCAACCTCAGCGTCGTTAATGGCAACGCGCGGGGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCa                                                                             <  2:192688/130‑1 (MQ=255)
                                                                                          cccAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTaaa                                                         <  6:141554/149‑1 (MQ=255)
                                                                                          cccAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTaa                                                          >  2:176765/1‑148 (MQ=255)
                                                                                          cccAACCTCAGAGTCGTTAATGGCAACGCTCCGTGTGTGCAGGGTGGCCTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTAc                                                                                                                           >  1:242578/1‑83 (MQ=255)
                                                                                               ccTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGt                                                    <  7:152898/149‑1 (MQ=255)
                                                                                                 tCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGtt                                                   >  2:90825/1‑148 (MQ=255)
                                                                                                  cAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGTTaa                                                 <  5:71266/149‑1 (MQ=255)
                                                                                                          ttAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGa                                                                                                                      <  7:70283/72‑1 (MQ=255)
                                                                                                          ttAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGa                                                                                                                      >  8:70283/1‑72 (MQ=255)
                                                                                                             aTGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAAtt                                      >  4:109285/1‑149 (MQ=255)
                                                                                                                gCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGc                                    <  7:125071/148‑1 (MQ=255)
                                                                                                                gCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCg                                   >  2:114237/1‑149 (MQ=255)
                                                                                                                gCAACGCTCAGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGc                                    <  5:45080/148‑1 (MQ=255)
                                                                                                                  aaCGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGc                                 >  7:35927/1‑149 (MQ=255)
                                                                                                                     gCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTttatt                                                                                                          <  1:25984/73‑1 (MQ=255)
                                                                                                                     gCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTttatt                                                                                                          >  2:25984/1‑73 (MQ=255)
                                                                                                                       tCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGCCTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCttt                            >  5:232987/1‑149 (MQ=255)
                                                                                                                       tCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTa                                                                                           <  6:132756/86‑1 (MQ=255)
                                                                                                                       tCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTa                                                                                           >  5:132756/1‑86 (MQ=255)
                                                                                                                         ggTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCttttt                          <  3:234375/149‑1 (MQ=255)
                                                                                                                         ggTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCttttt                          >  2:91758/1‑149 (MQ=255)
                                                                                                                           ggtgtgCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCGGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCtttt                           <  5:284554/145‑1 (MQ=255)
                                                                                                                               tgCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCAtt                     <  1:114237/148‑1 (MQ=255)
                                                                                                                                gCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCt                   >  2:203625/1‑149 (MQ=255)
                                                                                                                                      tGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGtt             >  2:60812/1‑149 (MQ=255)
                                                                                                                                         aCTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCa          <  1:90825/149‑1 (MQ=255)
                                                                                                                                             ccGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGtct      >  7:48161/1‑149 (MQ=255)
                                                                                                                                             ccGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGtc       <  6:240244/148‑1 (MQ=255)
                                                                                                                                                 aaCTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTAc  >  6:331854/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
ACTGGCACCGCTTGGCGATCTGGTCGGTGTTAACCGTCAGGTTACCGTGCTGGCGTTCCAGTTTGGTGATGGCTTCAGTCACATCATTTACCCAACCTCAGCGTCGTTAATGGCAACGCTCGGTGTGTGCAGGGTGGACTTCCGTAACTGGCTGAAGGTGGGCGCGACCCTACTTGGACTGCTGTTTATTATGTCCAGCGTCGTAGTGATCGGCGCTCAGTTGATGGGCTACCACTAAAAATGTTAAGAGCCGCAATTGCGGCTCTTTTTTCATTCTGCCGTTTCAGTCTCTAC  >  NC_000913/2418364‑2418657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: