Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	NC_000913	2,100,210	Δ12 bp	24.8%	coding (1048‑1059/1407 nt)	gnd ←	6‑phosphogluconate dehydrogenase, decarboxylating

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 2100209	38 (0.440)	12 (0.140)	10/260	NT	24.8%	coding (1060/1407 nt)	gnd	6‑phosphogluconate dehydrogenase, decarboxylating
	?	NC_000913	2100222 =	35 (0.400)					coding (1047/1407 nt)	gnd	6‑phosphogluconate dehydrogenase, decarboxylating

ATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGATTTTGCCCAGATACAGCGCACGACGAACTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTT  >  NC_000913/2100075‑2100368                                                                                                                                                    |                                                                                                                                                   agcGGCAATTTGCTGGACGAAGGTAGCAAGCAACAGGTTAGGTATCTGT‑GATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCa                                                                                                                                                  <  6:280422/147‑1 (MQ=255)  tCGGCAATTTGCTTGATGTACGGAGCCAGAAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATcc                                                                                                                                                    >  5:156539/1‑147 (MQ=255)  tCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATcc                                                                                                                                                    >  7:273675/1‑147 (MQ=255)     gCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGtt                                                                                                                                               <  5:101901/149‑1 (MQ=255)       gtagtcatcggcaatttgcttgaagtacggagccagcaacaggttagggatctgtggattttcggcataagcagcggtgattttctgcaggaactgcgcacggatgatgcagccagcacggaaaatcttcgcgatttcgccCCAGtt                                                                                                                                               <  4:125247‑M2/6‑1 (MQ=255)              tcggcaatttgcttgaagtacggagccagcaacaggttagcgatctgtggattttcggcataagcatcggtgattttctgcaggaactgcgcacggatgatgcagccagcacggaaaatcttcgcgatttcgccCCAGTTGTACTCtt                                                                                                                                       >  4:42932‑M2/135‑148 (MQ=255)                    tACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCa                                                                                                                                     <  2:27778/144‑1 (MQ=255)                    tACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCa                                                                                                                                     >  1:27778/1‑144 (MQ=255)                         aGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGAATTCGCCGTAGTTCAGATCCCAGATGTACTCTTCAGacgcagcac                                                                                                                           >  5:252357/1‑149 (MQ=255)                           ccAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGacgcagcacgc                                                                                                                         >  2:299421/1‑149 (MQ=255)                                   aGGTTAGCGATCTGTGGATTTTCGGCATACGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTga                                                                                                                   >  4:334668/1‑147 (MQ=255)                                                 ttgATTTTCGGCCTAAGGATCTGTGAGTTTCGGCAGTAACTGCGCACGGATGATGCAGCCAGCACGGAAAAGCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTgg                                                                                                       <  5:127354/143‑1 (MQ=255)                                                 tgGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTgg                                                                                                       <  6:156539/145‑1 (MQ=255)                                                 tgGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTg                                                                                                        <  1:203086/144‑1 (MQ=255)                                                   gATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTaa                                                                                                 <  4:310330/149‑1 (MQ=255)                                                    agtttCGGCATAAGCATCGGTGATTTTCTGCAGGACCTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTaa                                                                                                 <  5:368657/146‑1 (MQ=255)                                                      tttCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGATGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGa                                                                                               >  3:188306/1‑148 (MQ=255)                                                      tttCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGa                                                                                               >  7:208146/1‑148 (MQ=255)                                                            cATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGAtt                                                                                        >  1:159397/1‑149 (MQ=255)                                                             tggattttcggcataagcatcggtgattttctgcaggaactgcgcacggatgatgcagccagcacggaaaatcttcgcgatttcgccCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGaaa                                                                                             <  7:93997‑M2/56‑1 (MQ=255)                                                                    tCGGTGATTTTCTGCAGGCACTGCGCACGGATGCTGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCAGGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGATTTTGCCCAg                                                                                <  1:85200/149‑1 (MQ=255)                                                                       gcataagcatcggtgattttctgcaggaactgcgcacggatgatgcagccagcacggaaaatcttcgcgatttcgccCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGATTTTGCCCAGATa                                                                             >  3:222495‑M2/78‑149 (MQ=255)                                                                       gcataagcatcggtgattttctgcaggaactgcgcacggatgatgcagccagcacggaaaaacttcgcgatttcgccCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGATTTTGCCCAGATa                                                                             >  8:93685‑M2/78‑149 (MQ=255)                                                                       gTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGAtt                                                                                        >  1:165289/1‑138 (MQ=255)                                                                       gTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGAtt                                                                                        <  2:165289/138‑1 (MQ=255)                                                                       gTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGATTTTGCCCAGATa                                                                             <  5:132330/149‑1 (MQ=255)                                                                         gATTGTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGAGCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGATTTTGCCCAGATACa                                                                           <  4:82992/149‑1 (MQ=255)                                                                             gcatcggtgattttctgcaggaactgcgcacggatgatgcagccagcacggaaaatcttcgcgatttcgccCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGATTTTGCCCAGATACAgcg                                                                        >  7:374511‑M2/72‑148 (MQ=255)                                                                                tGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGATTTTGCCCAGATACAGCGCac                                                                     >  5:316866/1‑148 (MQ=255)                                                                                   aGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGATTTTGCCCAGATACAGCGCacgac                                                                  >  2:388798/1‑148 (MQ=255)                                                                                         tGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGATTTTGCCCAGATACAGCGCACGACGAACttt                                                           >  3:265084/1‑149 (MQ=255)                                                                                          gcgcACGGATGGTGCAGCCAGCACGGAAAATCTTCGCGATATCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGATTTTGCCCAGATACAGCGCACGACGAACtttt                                                          >  4:191936/1‑149 (MQ=255)                                                                                            gcACGGATGATGCAGCCAGCACGGAAAATCTTCTCGATTTCGCCGTTGTTCAGATCCCAGTTGTTCTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGATTTTGCCCAGATACAGCGCACGACGAACTTTTTc                                                        <  6:252357/149‑1 (MQ=255)                                                                                            gcACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAAc                                                                                            <  4:364636/113‑1 (MQ=255)                                                                                            gcACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAAc                                                                                            >  3:364636/1‑113 (MQ=255)                                                                                                      tgCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGATTTTGCCCAGATACAGCGCACGACGAACTTTTTCGATGAACTCa                                              <  4:259117/149‑1 (MQ=255)                                                                                                        gcacggatgatgcagccagcacggaaaatcttcgcgatttcgccCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGATTTTGCCCAGATACAGCGCACGACGAACTTTTTCGATGAACTCAGc                                            <  4:376300‑M2/105‑1 (MQ=255)                                                                                                         agccagcACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGATTTTGCCCAGATACAGCGCACGACGAACTTTTTCGATGAACTCAGcc                                           <  1:299421/149‑1 (MQ=255)                                                                                                         agccagaACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCATCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGATTTTGCCCAGATACAGCGCACGACGAACTTTTTCGATGAACTCAGcc                                           <  8:166182/149‑1 (MQ=255)                                                                                                           cggatgatgcagccagcacggaaaatcttcgcgatttcgccCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGATTTTGCCCAGATACAGCGCACGACGAACTTTTTCGATGAACTCAGCCtt                                         <  3:42932‑M2/108‑1 (MQ=255)                                                                                                                 cGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGATTTTGCCCAGATACAGCGCACGACGAACTTTTTCGATGAACTCAGCCTTGTCGcc                                   >  2:198270/1‑149 (MQ=255)                                                                                                                  tgcagccagcacggaaaatcttcgcgatttcgccCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGATTTTGCCCAGATACAGCGCACGACGAACTTTTTCGATGAACTCAGCCTTGTCGCct                                  <  8:374511‑M2/115‑1 (MQ=255)                                                                                                                   gAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGATTTTGCCCAGATACAGCGCACGACGAACTTTTTCGATGAACTCAGCCTTGTCGCctg                                 >  1:171074/1‑149 (MQ=255)                                                                                                                     agccagcacggaaaatcttcgcgatttcgccCCAGTTGTACTCTTCAGCCGCCGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGATTTTGCCCAGATACAGCGCACGACGAACTTTTTCGATGAACTCAg                                             >  7:268855‑M2/32‑135 (MQ=255)                                                                                                                         agcacggaaaatcttcgcgatttcgccCCAGTTGTACTCTTCAGACGCCGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGATTTTGCCCAGATACAGCGCACGACGAACTTTTTCGATGAACTCAGCCTTGTCGCCtgctggcg                           <  6:179476‑M2/122‑2 (MQ=255)                                                                                                                             gcgATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGATTTTGCCCAGATACAGCGCACGACGAACTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGc                       <  1:388798/149‑1 (MQ=255)                                                                                                                                     gCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGATTTTGCCCAGATACAGCGCACGACGAACTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGAc               <  5:221501/149‑1 (MQ=255)                                                                                                                                      ccGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGATTTTGCCCAGATACAGCGCACGACGAACTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGAcc              <  1:198270/149‑1 (MQ=255)                                                                                                                                         gcgatttcgccCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGATTTTGCCCAGATACAGCGCACGACGAACTTTTTCGATGAACTCAGCATTGTCGCCTGCTGGCTGTGCTTGCGGACCa             >  6:211301‑M2/12‑147 (MQ=255)                                                                                                                                            ttCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGATTTTGCCCAGATACAGCGCACGACGAACTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCaga           >  5:119480/1‑146 (MQ=255)                                                                                                                                                gATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGATTTTGCCCAGATACAGCGCACGACGAACTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACt    <  3:191936/149‑1 (MQ=255)                                                                                                                                                 aTCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGATTTTGCCCAGATACAGCGCACGACGAACTTTTTCGATGAACTCa                                              >  7:238898/1‑106 (MQ=255)                                                                                                                                                 aTCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGATTTTGCCCAGATACAGCGCACGACGAACTTTTTCGATGAACTCa                                              <  8:238898/106‑1 (MQ=255)                                                                                                                                                 aTCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGATTTTGCCCAGATACAGCGCACGACGAACTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACtt   <  5:158818/149‑1 (MQ=255)                                                                                                                                                  ccCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGATTTTGCCCAGATACAGCGCACGACGAACTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCg                  >  2:168224‑M2/3‑133 (MQ=255)                                                                                                                                                  ccCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGATTTTGCCCAGATACAGCGCACGACGAACTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCg                  <  1:168224‑M2/131‑1 (MQ=255)                                                                                                                                                    ccAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGATTTTGCCCAGATACAGCGCACGACGAACTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACttt  <  1:187663/147‑1 (MQ=255)                                                                                                                                                    ccAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGATTTTGCCCAGATACAGCGCACGACGAACTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACttt  >  2:187663/1‑147 (MQ=255)                                                                                                                                                    |                                                                                                                                                   ATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAACGATTTTGCCCAGATACAGCGCACGACGAACTTTTTCGATGAACTCAGCCTTGTCGCCTGCTGGCTGTGCTTGCGGACCAGAGAGAACTTT  >  NC_000913/2100075‑2100368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.