Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC NC_000913 257,908 Δ776 bp 100% [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 257908–258674 258683 10–776 113 [0] [1] 115 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)94 (1.150) 74/246 NT 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

Only 100 of 113 total aligned reads displayed.

GCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAAT  >  NC_000913/257761‑258054
                                                                                                                                                  |                                                                                                                                                   
gCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTTCATCACAACAGGAGATAGAAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACAGCACTAGGCCCGTATATTCGTGAAGGTAag                                                                                                                                                   <  3:202165‑M1/149‑3 (MQ=255)
 cTTGTGTTAAAAATTTTCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTTCCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagt                                                                                                                                                  <  1:355792‑M1/149‑4 (MQ=255)
 cTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACATTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagt                                                                                                                                                  >  6:293792‑M1/1‑146 (MQ=255)
     tgtTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaa                                                                                                                                              <  8:245373‑M1/149‑8 (MQ=255)
              tttGCTAAATTTTGCCAATTTGCAAAAACACTTGCATCACAACAGGAGATAGCAATGACGATACCGAGTCGACACCCGAAGAGCACAATCATCAAAAAAATTACCTCACTAGCCCCGTATATTCGTGAAGGTAagtgcaaagataatc                                                                                                                                      >  8:120771‑M1/1‑133 (MQ=255)
                   tAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattct                                                                                                                                >  1:16004‑M1/1‑128 (MQ=255)
                   tAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattct                                                                                                                                >  5:9846‑M1/1‑128 (MQ=255)
                       ttttGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattcttttt                                                                                                                            <  2:316124‑M1/149‑26 (MQ=255)
                            ccAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgat                                                                                                                        <  4:231240‑M1/148‑30 (MQ=255)
                             cAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattg                                                                                                                      >  1:342914‑M1/1‑118 (MQ=255)
                              aaTTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgt                                                                                                                     >  7:307867‑M1/1‑117 (MQ=255)
                              aaTTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACACGAAGAGCAGATTGATCAAAAAATTTACCGCACAAGGCCCGTATATTCGTGAACGTAagtgcaaagataatcgattctttttcgattg                                                                                                                      >  1:195736‑M1/1‑117 (MQ=255)
                               aTTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtc                                                                                                                    >  4:286451‑M1/1‑116 (MQ=255)
                               aTTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtc                                                                                                                    <  3:83332‑M1/149‑34 (MQ=255)
                                  tGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTTGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctg                                                                                                                  <  5:59130‑M1/148‑36 (MQ=255)
                                   ggTAAAACAGTTGCATCACAACAGGAGATACCAATGCCGTTACCGAGTTGACACCCGAAGAGCAGATTGCTCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctgg                                                                                                                 <  3:258943‑M1/148‑37 (MQ=255)
                                        aaCAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataat                                                                                                                                       <  3:296746‑M1/121‑15 (MQ=255)
                                        aaCAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataat                                                                                                                                       >  4:296746‑M1/1‑107 (MQ=255)
                                          cAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgc                                                                                                         >  7:402569‑M1/1‑105 (MQ=255)
                                          cAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgc                                                                                                         <  8:137714‑M1/149‑45 (MQ=255)
                                            gTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgt                                                                                                       >  6:138798‑M1/1‑103 (MQ=255)
                                               gCATCACAACAGGAGATAGCAATGAAGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaa                                                                                                    >  3:368772‑M1/1‑100 (MQ=255)
                                                 aTCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacg                                                                                                  >  8:210453‑M1/1‑98 (MQ=255)
                                                      aacaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaa                                                                                             >  2:164922‑M1/1‑93 (MQ=255)
                                                      aacaGGAGATAGCAATGACATTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaa                                                                                             <  5:293792‑M1/149‑57 (MQ=255)
                                                         aGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcatcaacgtgaaaccg                                                                                          >  6:229899‑M1/1‑90 (MQ=255)
                                                          ggAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGACCGTAGATTCGTGAAGGTTagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaacctg                                                                                         >  4:237448‑M1/1‑89 (MQ=255)
                                                           gagaTAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggc                                                                                        <  5:307389‑M1/149‑62 (MQ=255)
                                                              aTAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgcaaccggcacc                                                                                     >  5:32997‑M1/1‑85 (MQ=255)
                                                                   aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaag                                                                                >  1:212820‑M1/1‑80 (MQ=255)
                                                                   aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcac                                                                                      <  7:2391‑M1/143‑64 (MQ=255)
                                                                   aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcac                                                                                      >  8:2391‑M1/1‑80 (MQ=255)
                                                                    aTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagt                                                                               <  7:228963‑M1/149‑71 (MQ=255)
                                                                       aCGTTACCGAGTGGCCACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcggcaacgtgaaaccggcaccggaagtgc                                                                             <  2:309294‑M1/148‑73 (MQ=255)
                                                                        cGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgt                                                                           <  2:210649‑M1/149‑75 (MQ=255)
                                                                        cGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcggcaacgtgaaaccggcaccggaagtgcgt                                                                           <  6:298824‑M1/149‑75 (MQ=255)
                                                                             ccGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaatt                                                                      >  3:337797‑M1/1‑70 (MQ=255)
                                                                              cGAGTGGACACCCGAAGAGGAGATTGATCAAAAAATTTACCGCACTAGGCCGGTATATTCGTGAAGGTAagtgcaaagattatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattc                                                                     <  5:179737‑M1/149‑81 (MQ=255)
                                                                                 gTGGACCCCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctgtttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggcagtgcgtgaagtctgg                                                                  >  8:251023‑M1/1‑66 (MQ=255)
                                                                                       acCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctgg                                                            <  2:342914‑M1/149‑90 (MQ=255)
                                                                                       acCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctgg                                                                  <  8:241953‑M1/143‑84 (MQ=255)
                                                                                       acCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctgg                                                                  >  7:241953‑M1/1‑60 (MQ=255)
                                                                                         ccGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtg                                                          >  6:373289‑M1/1‑58 (MQ=255)
                                                                                         ccGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtg                                                          >  3:126509‑M1/1‑58 (MQ=255)
                                                                                         ccGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccg                                                                                          <  3:230667‑M1/117‑60 (MQ=255)
                                                                                         ccGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccg                                                                                          >  4:230667‑M1/1‑58 (MQ=255)
                                                                                         ccGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctattacgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtg                                                          >  8:368150‑M1/1‑58 (MQ=255)
                                                                                         ccGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCACGTATATTCGTGAAGGTAagtgcaaagataatcgattcttattcgattgtctggctgtatgcgtaaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtg                                                          >  4:186834‑M1/1‑58 (MQ=255)
                                                                                          cGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtgg                                                         <  5:160947‑M1/149‑93 (MQ=255)
                                                                                             agagCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatg                                                      <  3:286451‑M1/149‑96 (MQ=255)
                                                                                                 cAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtga                                                                                               <  1:225561‑M1/104‑55 (MQ=255)
                                                                                                 cAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtga                                                                                               >  2:225561‑M1/1‑50 (MQ=255)
                                                                                                  aGATTGATCAAAAAATTTAGCGCTCTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattttctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgatttctggggctggtggatggagct                                                 <  2:195736‑M1/149‑101 (MQ=255)
                                                                                                   gATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctg                                                <  8:307867‑M1/149‑102 (MQ=255)
                                                                                                   gATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctg                                                >  7:271945‑M1/1‑48 (MQ=255)
                                                                                                       gATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctgtaa                                             <  5:222996‑M1/148‑105 (MQ=255)
                                                                                                       gATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaag                                            >  1:192255‑M1/1‑44 (MQ=255)
                                                                                                        aTCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagc                                           >  5:330942‑M1/1‑43 (MQ=255)
                                                                                                        aTCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagc                                           >  5:232628‑M1/1‑43 (MQ=255)
                                                                                                         tCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcg                                          >  6:290072‑M1/1‑42 (MQ=255)
                                                                                                         tCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtagggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcg                                          >  6:42378‑M1/1‑42 (MQ=255)
                                                                                                           aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggtaccggaagtgcgtgaattctggggctggtggagggagctggaagcg                                          >  8:154436‑M1/1‑40 (MQ=255)
                                                                                                           aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgca                                        <  4:337797‑M1/149‑110 (MQ=255)
                                                                                                           aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcg                                          >  7:377910‑M1/1‑40 (MQ=255)
                                                                                                           aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcg                                          >  1:60359‑M1/1‑40 (MQ=255)
                                                                                                           aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcg                                          >  3:194493‑M1/1‑40 (MQ=255)
                                                                                                            aaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctgggtgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcag                                       <  1:61709‑M1/149‑111 (MQ=255)
                                                                                                            aaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcag                                       >  3:184231‑M1/1‑39 (MQ=255)
                                                                                                            aaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcatcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcag                                       <  5:229899‑M1/149‑111 (MQ=255)
                                                                                                               aaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagggcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaa                                    <  7:300167‑M1/149‑114 (MQ=255)
                                                                                                                    aCCGCACTAGGCCCGTATATTCGTGAAGGTAagttcaaagataatcgattctttttcgattgtctggctgtatgcgtcaaagtgaaacctgcaccggaattgagtgaattctggggctggtggatggagctggaagcgcaggaatcccg                               >  1:162121‑M1/1‑31 (MQ=255)
                                                                                                                    aCCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctgggcctggtggatggagctggaagcgcaggaatccc                                <  5:138798‑M1/148‑118 (MQ=255)
                                                                                                                        cACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgtttt                           <  7:321000‑M1/149‑123 (MQ=255)
                                                                                                                         aCTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgtttta                          >  3:105698‑M1/1‑26 (MQ=255)
                                                                                                                          cTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttac                         <  4:368772‑M1/149‑125 (MQ=255)
                                                                                                                           tAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctgtctgtatgcgtcaacgtgaaaccggaacctgaagtgcgtgaattatggggctggtggatcgagctggaagcgcagtaataccgttttac                         >  4:19881‑M1/1‑24 (MQ=255)
                                                                                                                           tAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacc                        >  1:21459‑M1/1‑24 (MQ=255)
                                                                                                                             ggCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttaccta                      <  1:164922‑M1/149‑128 (MQ=255)
                                                                                                                             ggCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacc                        <  3:225342‑M1/147‑126 (MQ=255)
                                                                                                                               cccGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctaca                    <  3:19881‑M1/149‑130 (MQ=255)
                                                                                                                                ccGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctg                                                             <  8:310934‑M1/107‑89 (MQ=255)
                                                                                                                                 cGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagt                  >  4:228185‑M1/1‑18 (MQ=255)
                                                                                                                                   tataTTCGTGAAGGTAagtgcaaagataatcgattcgttttcgattgtgtggctgtatgcgtcaacgtgaaacagccagcggaagtgcgtgaattctggggctggtggatggagatggaagcgcaggaagcccgttttacctaccgtta                >  5:143130‑M1/1‑16 (MQ=255)
                                                                                                                                    atatTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttac               >  7:391530‑M1/1‑15 (MQ=255)
                                                                                                                                     tatTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcgcgttttacctacagttac               <  4:194493‑M1/148‑135 (MQ=255)
                                                                                                                                     tatTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttac               >  4:206998‑M1/1‑14 (MQ=255)
                                                                                                                                     tatTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgtttt                           >  1:92173‑M1/1‑14 (MQ=255)
                                                                                                                                      atTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctgggg                                                                >  2:50119‑M1/1‑13 (MQ=255)
                                                                                                                                       ttCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttacca             <  1:364688‑M1/148‑137 (MQ=255)
                                                                                                                                       ttCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttac               >  2:364688‑M1/1‑12 (MQ=255)
                                                                                                                                         cGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtt          >  7:158864‑M1/1‑10 (MQ=255)
                                                                                                                                         cGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttacaggtt          >  3:36820‑M1/1‑10 (MQ=255)
                                                                                                                                           ttaaggTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttg        <  4:105698‑M1/147‑142 (MQ=255)
                                                                                                                                             aaGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggt      <  5:373289‑M1/149‑144 (MQ=255)
                                                                                                                                             aaGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggt      >  3:144230‑M1/1‑6 (MQ=255)
                                                                                                                                             aaGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggt      <  7:210453‑M1/149‑144 (MQ=255)
                                                                                                                                             aaGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggt      <  6:9846‑M1/149‑144 (MQ=255)
                                                                                                                                               ggTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtct    <  2:16004‑M1/149‑146 (MQ=255)
                                                                                                                                                gTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctg   >  4:219602‑M1/1‑3 (MQ=255)
                                                                                                                                                 tAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgt  <  4:184231‑M1/149‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAAT  >  NC_000913/257761‑258054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: