| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 675,414 | C→A | 24.3% | H133N (CAT→AAT) | ybeL → | DUF1451 family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 675,414 | 0 | C | A | 24.3% | 45.1 / 5.1 | 27 | H133N (CAT→AAT) | ybeL | DUF1451 family protein |
| Reads supporting (aligned to +/- strand): ref base C (16/4); new base A (2/5); total (18/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.35e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGCAGGAGCTGGCAGACATCACCGATAAAACGCAGCTTGAATGGCGCGAAGTTTTCCAGGACCTCAATCATCATGGGGTTTATCACAGCGGAGAAGTGGTCGGGCTGGGAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGT > NC_000913/675276‑675561 | tgGCAGGAGCTGGCAGACATCACAGATAAAACGCAGCTTGAATGGCGCGAAGTTTTCCAGGACCTCAATCATCATGGGGTTTATCACAGCGGAGAAGTGGTCGGGCTGGGAAATCTGGGCTGCGAGAAATGTCACTTCCATCTCCCGAt < 4:2310/149‑1 (MQ=255) cAGGAGCTGGCAGACATCACCGATAAAACGCAGCTTGAATGGCGCGAAGTTTTCCAGGACCTCAATCATCATGGGGTTTATCACAGCGGAGAAGTGGTCGGGCTGGGAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTa > 3:194431/1‑149 (MQ=255) aTCACCGATAAAACGCAGCTTGAATGGCGCGAAGTTTTCCAGTACCTCAATCATCATGTGGTTTATCACAGCGGAGAAGTGGTCGGGCTGGGAAATCTGGTATTCGAGCAATGTCACTTACATCTCCCGATCTACACACCGGAAGTGCt > 4:308173/1‑149 (MQ=255) aCCGATAAAACGCAGCTTGAATGGCGCGAAGTTTTCCAGGACCTCAATCATCATGGGTTTTATCACAGCGGAGAAGTGGTCGGGCTGGGAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGAc > 6:348096/1‑149 (MQ=255) aCCGATAAAACGCAGCTTGAATGGCGCGAACTTTTCCAGGACCTCAATCATCATGGGGTTTATCACAGCGGAGAAGTGGTCGGGCTGGGAAATCTGGTCTGCGAGAAATTTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGAc > 7:187501/1‑149 (MQ=255) gCAGCTTGAATGGCGCGAAGTTTTCCAGGACCTCAATCATCATGGGGTTTATCACAGCGGAGAAGTGGTCGGGCTGGGAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGCCCg > 4:205470/1‑148 (MQ=255) gCTTGAATGGCGCGAAGTTTTCCAGGACCTCAATCATCATGGGGTTTATCACAGCGGAGAAGTGGTCGCGCGGGGAAATATGGTCTGCGAGAACTGTCACTTCCATCTCCACATATACACACCGAAAGTCCAGACGCAATCCCCGaaag > 7:302709/1‑148 (MQ=255) gttGAATGGCGCGAAGTTTTCCAGGACCTCAATCATCATGGGGTTTATCACAGCGGAGAAGTGGTCGGGCTGGGAAATCTGGTCTGAGAGAAATGTCACTTCAATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAAtg > 5:223850/2‑149 (MQ=255) taaTGGCGCGAAGTTTTCCAGGAGCTCAATCATCATGGGGTTTATCACAGCGGAGAAGGGGTCGGGCTGGGAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAAtgtg < 3:308173/147‑1 (MQ=255) ctcgAAGCTATCCAGGACCTCAATCATCAAGGGCTTTATCACAGCGGAGAAGTGGTCTGGCTGGGAAATATTTTTTTTTTTAATGTTAAGTTGAATATCGAGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGGTCATGa < 8:187501/147‑1 (MQ=255) gAAGTTTTCCAGGACCTCAATCATCATGGGGTTTATCACAGCGGAGAAGTGGTCGGGCTGGGAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGATATGCCCTAAATGTGGTAATGACCa > 5:227055/1‑149 (MQ=255) ttCCAGGACCTCAATCATCATGGGGTTTATCACAGCGGAGAAGTGGTCGGGCTGGGAAATCTGGTCAGCGAGAAATGTCACTTCCATCTCCCGAACTACACACCGCAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACAACTTCCa > 1:163867/1‑149 (MQ=255) cAGGACCTCAATCATCATGGGGTTTATCACAGCGGAGAAGTGGTCGGGCTGGGAAATCTGGTCTGCGAGAATTGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTcaa > 1:313591/1‑144 (MQ=255) aGGACCTCAATCATCATGGGTTTTATCACAGCTGAGAAGTTGTCGGGCTTGGAAATATTGTATGTTTTAAATTTAACTTGAATATCGAGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAgaga < 4:194431/149‑1 (MQ=255) cTCAATCATCATGGGGTTTATCACAGCGGAGAAGTGGTCGGGCTGCGAAATCTGGGATGCCAGAAATGTCACTTCAATATCCCGATCTACACACCGGAAGTGCTGACGATATGCCCGAAATCTGGTCATGACCAGTTCAAGAGActcac > 1:126567/1‑145 (MQ=255) ggggTTTATCACAGCGGAGAAGTGGTCGGGCTGGGAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGACCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGt > 1:175185/1‑148 (MQ=255) gttttATCACAGCGGAGAAGTGGTCGGGCTTGTAAATCTTTTTTTTTTTAATTTTAAGTTGAATATCGAGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTaa < 2:175185/146‑1 (MQ=255) gTTTATCACAGCGTAGAAGTGGTCGGGATGTGAAATCTTTTTTTTTTTAATTTTAAGTTGAATATCGAGATCTACACACCGGAAGTGCTGACGCTATGACCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATa < 2:313591/149‑1 (MQ=255) agaAGTGGTCGGGCTGGGAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCAATGCCCGAAATGTCGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTATGATTTAGca > 4:147754/1‑148 (MQ=255) tGGTCGGGCTGGGAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACTCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGGCTGATTTCGCACAGCaa < 5:348096/149‑1 (MQ=255) gTCGGGCTGGGAAATCTGTTTTTTGTTAAATTTAAGTTGAATATCGAGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAAt < 6:227055/148‑1 (MQ=255) gAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCg < 2:94256/38‑1 (MQ=255) gAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCg > 1:94256/1‑38 (MQ=255) tGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTAGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATg > 3:194639/1‑148 (MQ=255) gagaAATGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAAt > 2:282189/1‑149 (MQ=255) aTGTCCCTTCCATCTCCCGATCTACACACCGGAAGTCCAGACGCTATGCCCGAAATGTGCTGATGACCAGTTCCAGCGACGCCCGGTTCAGCCGTAATAGACTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTcccc > 5:46958/1‑149 (MQ=255) tCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGc > 4:112265/1‑149 (MQ=255) ccATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGt > 2:82491/1‑149 (MQ=255) | TGGCAGGAGCTGGCAGACATCACCGATAAAACGCAGCTTGAATGGCGCGAAGTTTTCCAGGACCTCAATCATCATGGGGTTTATCACAGCGGAGAAGTGGTCGGGCTGGGAAATCTGGTCTGCGAGAAATGTCACTTCCATCTCCCGATCTACACACCGGAAGTGCTGACGCTATGCCCGAAATGTGGTCATGACCAGTTCCAGAGACGCCCGTTTGAGCCGTAATAGTCTGATTTCGCACAGCAATAAAGAGAATATGGGCCGAATGAAATTCCCCGGCCCTTGT > NC_000913/675276‑675561 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |