Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,009,106 T→C 5.0% Y421Y (TAT→TAC)  rlmL → 23S rRNA m(2)G2445 and m(7)G2069 methyltransferases, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,009,1060TC 5.0% 246.2 / 2.7 79Y421Y (TAT→TAC) rlmL23S rRNA m(2)G2445 and m(7)G2069 methyltransferases, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (39/36);  new base C (2/2);  total (41/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01

CGTACAGAAAAATTACCACGTTGCCGAAAGCACTCCAGACAGCAAACCGGCGATGGTAGCGGAAGACTACACCAACCGCCTGCGTAAGAACCTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAGCGCGTCAGCGTCTGTTCGATATTATCGCTGCAACCATTTCGGTACTGG  >  NC_000913/1008959‑1009254
                                                                                                                                                   |                                                                                                                                                    
cGTACAGAAAAATTACCACGTTGCCGAAAGCACTCCAGACAGCAAACCGGCGATGGTAGCGGAAGACTACACCAACCGCCTGCGTAAGAACCTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTAt                                                                                                                                                      <  3:319049/148‑1 (MQ=255)
     aGAAAAATTACCACGTTGCCGAAAGCACTCCAGACAGCAAACCGGCGATGGTAGCGGAAGACTACACCAACCGCCTGCGTAAGAACCTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGcc                                                                                                                                                >  8:14176/1‑149 (MQ=255)
       aaaaaTTACCACGTTGCCGAAAGCACTCCAGACAGCAAACCGGCGATGGTAGCGGAAGACTACACCAACCGCCTGCGTAAGAACCTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACg                                                                                                                                                  <  5:280826/145‑1 (MQ=255)
       aaaaaTTACCACGTTGCCGAAAGCACTCCAGACAGCAAACCGGCGATGGTAGCGGAAGACTACACCAACCGCCTGCGTAAGAACCTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACg                                                                                                                                                  >  6:280826/1‑145 (MQ=255)
        aaaaTTACCACGTTGCCGAAAGCACTCCAGACAGCAAACCGGCGATGGTAGCGGAAGACTACACCAACCGCCTGCGTAAGAACCTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGAt                                                                                                                                             <  6:320972/149‑1 (MQ=255)
            ttACCACGTTGCGGAAAGCACTCCAGAGAGCAAACCGGCGATGGTATCGGAAGACTACACCAACCGCCTGCGTAAGAACCTCAAAAACTTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGc                                                                                                                                         <  2:68842/149‑1 (MQ=255)
                cACGTTGCCGAAAGCACTCCAGACAGCAAACCGGCGATGGTAGCGGAAGACTACACCAACCGCCTGCGTAAGAACCTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAg                                                                                                                                      >  6:117017/1‑148 (MQ=255)
                cACGTTGCCGAAAGCACTCCAGACAGCAAACCGGCGATGGTAGCGGAAGACTACACCAACCGCCTGCGTAAGAACCTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAg                                                                                                                                      >  2:28526/1‑148 (MQ=255)
                 aCGTTGCCGAAAGCACTCCAGACAGCAAACCGGCGATGGTAGCGGAAGACTACACCAACCGCCTGCGTAAGAACCTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGaa                                                                                                                                    <  1:206191/149‑1 (MQ=255)
                  cGTTGCCGAAAGCACTCCAGACAGCAAACCGGCGATGGTAGCGGAAGACTACACCAACCGCCTGCGTAAGAACCTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAat                                                                                                                                   <  5:117017/149‑1 (MQ=255)
                    ttGCCGAAAGCACTCCAGACAGCAAACCGGCGATGGTAGCGGAAGACTACACCAACCGCCTGCGTAAGAACCTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAata                                                                                                                                  >  4:45225/1‑148 (MQ=255)
                       ccGAAAGCACTCCAGACAGCAAACCGGCGATGGTAGCGGAAGACTACACCAACCGCCTGCGTAAGAACCTCAAAAAATTCGAGAAGTGTGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATaa                                                                                                                               <  4:227182/148‑1 (MQ=255)
                         gAAAGCACTCCAGACAGCAAACCGGCGATGGTAGCGGAAGACTACACCAACCGCCTGCGTAAGAACCTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGt                                                                                                                            <  1:28526/149‑1 (MQ=255)
                              cACTCCAGACAGCAAACCGGCGATGGTAGCGGAAGACTACACCAACCGCCTGCGTAAGAACCTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCg                                                                                                                       <  1:16738/149‑1 (MQ=255)
                                 tCCAGACAGCAAACCGGCGATGGTAGCGGAAGACTACACCAACCGCCTGCGTAAGAACCTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGACAGAATATAACGTTGCCGtt                                                                                                                     >  8:216247/1‑148 (MQ=255)
                                     gACAGCAAACCGGCGATGGTAGCGGAAGACTACACCAACCGCCTGCGTAAGAACCTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCg                                                                                                                <  4:70136/149‑1 (MQ=255)
                                      aCAGCAAACCGGCGATGGTAGCGGCAGACTACACCAACCCCCAGCGTAAGAACCTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGAGTGAAGCTTACAGCCTGTATGACGCCGAGCTGCCAGAAAATAACGTTGCCGTGGACCGt                                                                                                               >  2:295851/1‑149 (MQ=255)
                                        aGCAAACCGGCGATGGTAGCGGAAGACTACACCAACCGCCTGCGTAAGAACCTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTa                                                                                                             >  7:128885/1‑149 (MQ=255)
                                          cAAACCGGCGATGGTAGCGGAAGACTACACCAACCGCCTGCGTAAGAACCTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGGTGAACGTTATg                                                                                                           >  4:228358/1‑149 (MQ=255)
                                                ggCGATGGTAGCGGAAGACTACACCAAACGCCTGCGTAAGAACCTCAAAAAATTCGAGAAGTTGGCTCGCCAGGAAGTGATTGAATGTTACCGCATGTATGACGCCGCTCTGCTAGAATATAACTTTGCCGTTGACCGTTATGCCgcat                                                                                                     >  2:229382/1‑146 (MQ=255)
                                                   gATGGTAGCGGAAGACTACACCAACCGCCTGCGTAAGAACCTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTggg                                                                                                  <  3:45225/149‑1 (MQ=255)
                                                        tAGCGGAAGACTACACCAACCGCCTGCGTAAGAACCTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGggtggtg                                                                                             <  3:29230/149‑1 (MQ=255)
                                                          gCGGAAGACTACACCAACCGCCTGCGTAAGAACCTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTAt                                                                                                                                                      >  2:352815/1‑90 (MQ=255)
                                                          gCGGAAGACTACACCAACCGCCTGCGTAAGAACCTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTAt                                                                                                                                                      <  1:352815/90‑1 (MQ=255)
                                                              aaGACTACACCAACCGCCTGCGTAAGAACCTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAg                                                                                       <  3:123263/149‑1 (MQ=255)
                                                                     cacCAACCGCCTGCGTAAGTACCTCAAAAAAATCGCGCAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTAt                                                                                 <  2:166897/148‑1 (MQ=255)
                                                                     cacCAACCGCCTGCGTAAGAACCTCAAAAAATTCGAGAAGTGGGCTCTCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATg                                                                                >  3:29746/1‑149 (MQ=255)
                                                                     cacCAACCGCCTGCGTAAGAACCTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAata                                                                                                                                  <  3:276966/99‑1 (MQ=255)
                                                                     cacCAACCGCCTGCGTAAGAACCTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTAt                                                                                 <  6:53532/148‑1 (MQ=255)
                                                                      acCAACCGCCTGCGTAAGATCCTCAAAAACTTCGAGATGGCGGCGCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGACTATAACGTTGCCGTTGACCGTTCTGCCGACTGGGTGGTGGTGCAGGAGTATgc                                                                               <  6:162290/149‑1 (MQ=255)
                                                                        cAACCGCCTGCGTAAGAACCTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATgcgc                                                                             >  6:120856/1‑149 (MQ=255)
                                                                          aCCGCCTGCGTAAGAACCTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAAAGTTACCGCCTGTATGACGACGCTCTGACAGAATAAAACGTTGCCGATGACCGTTATGACGACTCGGAGGTTGTGCAGGACTATGagccg                                                                           >  7:18222/1‑149 (MQ=255)
                                                                           ccGCCTGCGTAAGAACCTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGcgccgc                                                                          >  4:22090/1‑149 (MQ=255)
                                                                                tGCGTAAGAACCTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCaaa                                                                      >  1:38066/1‑148 (MQ=255)
                                                                                 gCGTAAGAACCTCAAAAAATTCGAGAAGTGGGCTCGCAAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCaaaaa                                                                    >  2:372054/1‑149 (MQ=255)
                                                                                  cGTAAGAACCTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCaaaaa                                                                    <  5:132733/148‑1 (MQ=255)
                                                                                  cGTAAGAACCTCAAAAAATTCCAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCaaaaa                                                                    <  5:241601/148‑1 (MQ=255)
                                                                                      aGAACCTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTAtt                                                               <  8:326050/149‑1 (MQ=255)
                                                                                      aGAACCTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTAt                                                                <  1:161141/148‑1 (MQ=255)
                                                                                      aGAACCTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTAACGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGACGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATTCGCAGCCAAAAACTAtt                                                               >  6:376794/1‑149 (MQ=255)
                                                                                       gAACCTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTAt                                                                                                            >  4:151061/1‑103 (MQ=255)
                                                                                       gAACCTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTAt                                                                                                            <  3:151061/103‑1 (MQ=255)
                                                                                           cTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGc                                                          >  6:379286/1‑149 (MQ=255)
                                                                                            tCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCt                                                         >  1:26627/1‑149 (MQ=255)
                                                                                            tCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTACGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCt                                                         >  4:77829/1‑149 (MQ=255)
                                                                                            tCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTACGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCt                                                         >  4:82966/1‑149 (MQ=255)
                                                                                            tCAAAAAATTAGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCt                                                         >  8:20635/1‑149 (MQ=255)
                                                                                              aaaaaaTTCGAGAAGTGGGCTCGCGAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGCCCGGTATGCCGAGTGGGTGGTGGTGCAGGAGTAGGCGCCGCCAAAAACTATTGATGCTca                                                       >  6:294537/1‑149 (MQ=255)
                                                                                               aaaaaTTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCTca                                                       >  3:727/1‑148 (MQ=255)
                                                                                                aaaaTTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGc                                                                                         >  6:88779/1‑113 (MQ=255)
                                                                                                aaaaTTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGc                                                                                         <  5:88779/113‑1 (MQ=255)
                                                                                                       gagaAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAGcgcg                                              >  1:162364/1‑149 (MQ=255)
                                                                                                       gagaAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGAGCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAgcgc                                               <  1:8708/148‑1 (MQ=255)
                                                                                                        agaAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAGCGCGt                                             >  6:162115/1‑149 (MQ=255)
                                                                                                                gCTCGCCAGGAAGGGATTGAATGTTACCGCCTCTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAGCGCGTCAGCGTCt                                     >  8:310963/1‑149 (MQ=255)
                                                                                                                  tCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAGCGCGTCAGCGTCTGt                                   >  2:279546/1‑149 (MQ=255)
                                                                                                                     ccAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAGCGCGTCAGCGTCTGTTc                                 >  3:358794/1‑148 (MQ=255)
                                                                                                                      cAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAGCGCGTCAGCGTCTGTTCGa                               <  2:162364/149‑1 (MQ=255)
                                                                                                                       aGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAGCGCGTCAGCGTCTGTTCGat                              <  2:26627/149‑1 (MQ=255)
                                                                                                                        ggAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAGCGCGTCAGCGTCTGTTCGata                             <  5:379286/149‑1 (MQ=255)
                                                                                                                          aaGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAGCGCGTCAGCGTCTGTTCGAtatt                           <  7:14176/149‑1 (MQ=255)
                                                                                                                              gATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAGCGCGTCAGCGTCTGTTCGATATTATCg                       >  2:81726/1‑149 (MQ=255)
                                                                                                                                ttGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGTGTATGCGCCGCCAAAAACTATTTTTGCTCACAAAGCGCGTCAGCGTCTGTTCGATATTATCGCt                     <  7:405344/149‑1 (MQ=255)
                                                                                                                                   aaTGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAGCGCGTCAGCGTCTGtt                                  <  5:76735/133‑1 (MQ=255)
                                                                                                                                   aaTGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAGCGCGTCAGCGTCTGtt                                  >  6:76735/1‑133 (MQ=255)
                                                                                                                                   aaTGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAGCGCGTCAGCGTCTGTTCGATATTATCGCTGc                   >  5:189950/1‑148 (MQ=255)
                                                                                                                                   aaTGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAGCGCGTCAGCGTCTGTTCGATATTATCGCTGc                   <  4:29746/148‑1 (MQ=255)
                                                                                                                                   aaTGTTACCGCCTGTATGACGCCGATCTGCCACAATATAACGATCCCGTTGACCGTTATGCCGACTCGCTGGTGGTGCAGGAGTAAGCGCAGCCAAAAACTAGTGATGCTCACAAAGCGAGTCAGCGACTGTTCGATATTATCgcagca                  >  6:194696/1‑149 (MQ=255)
                                                                                                                                    aTGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAGCGCGTCAGCGTCTGTTCGATATTATCGCTGCaa                 >  4:234450/1‑149 (MQ=255)
                                                                                                                                          ccGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAGCGCGTCAGCGTCTGTTCGATATTATCGCTGCAACCAttt           >  2:189848/1‑149 (MQ=255)
                                                                                                                                          ccGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAGCGCGTCAGCGTCTGTTCGATATTATCGCTGCAACCAttt           <  3:234450/149‑1 (MQ=255)
                                                                                                                                          ccGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGGGCAGGAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAGCGCGTCAGCGTCTGTTCGATATTATCGCTGCAACCAtt            <  1:279546/148‑1 (MQ=255)
                                                                                                                                          ccGCCTGTACGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAGCGCGTCAGCGTCTGTTCGATATTATCGCTGCAACCAttt           <  3:82966/149‑1 (MQ=255)
                                                                                                                                          ccGCCTGTACGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAGCGCGTCAGCGTCTGTTCGATATTATCGCTGCAACCAttt           <  3:77829/149‑1 (MQ=255)
                                                                                                                                           ctccTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAGCGCGTCAGCGTCTGTTCGATATTATCGCTGCAACCATTTc          <  1:372054/147‑1 (MQ=255)
                                                                                                                                            gCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAGCGCGTCAGCGTCTGTTCGATATTATCGCTGCAACCATTTCg         <  7:20635/149‑1 (MQ=255)
                                                                                                                                             ccTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAGCGCGTCAGCGTCTGTTCGATATTATCGCTGCAACCATTTCgg        >  5:184654/1‑149 (MQ=255)
                                                                                                                                              cTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAGCGCGTCAGCGTCTGTTCGATATTATCGCTGCAACCATTTCgg        >  4:370551/1‑148 (MQ=255)
                                                                                                                                               tGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAGCGCGTCAGCGTCTGTTCGATATTATCGCTGCAACCATTTCGGTa      >  2:268364/1‑149 (MQ=255)
                                                                                                                                                 tATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAGCGCGTCAGCGTCTGTTCGATATTATCGCTGCAACCATTTCGGTa      <  5:120856/147‑1 (MQ=255)
                                                                                                                                                 tATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAGCGCGTCAGCGTCTGTTCGATATTATCGCTGCAACCATTTCGGTAc     <  3:370551/148‑1 (MQ=255)
                                                                                                                                                 tATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAGCGCGTCAGCGTCTGTTCGATATTATCGCTGCAACCATTTCGGTACt    >  3:22418/1‑149 (MQ=255)
                                                                                                                                                   tGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAGCGCGTCAGCGTCTGTTCGATATTATCGCTGCAACCATTTCGGTACTgg  >  6:285028/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CGTACAGAAAAATTACCACGTTGCCGAAAGCACTCCAGACAGCAAACCGGCGATGGTAGCGGAAGACTACACCAACCGCCTGCGTAAGAACCTCAAAAAATTCGAGAAGTGGGCTCGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCAGAATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCTCACAAAGCGCGTCAGCGTCTGTTCGATATTATCGCTGCAACCATTTCGGTACTGG  >  NC_000913/1008959‑1009254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑