Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,066,845 G→C 5.0% intergenic (+19/+19) agp → / ← yccJ glucose‑1‑phosphatase/inositol phosphatase/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,066,8450GC 5.0% 276.7 / 5.9 80intergenic (+19/+19)agp/yccJglucose‑1‑phosphatase/inositol phosphatase/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (36/40);  new base C (2/2);  total (38/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.53e-01

CCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGT  >  NC_000913/1066699‑1066988
                                                                                                                                                  |                                                                                                                                               
ccGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCgag                                                                                                                                                 >  8:16380/1‑147 (MQ=255)
   aTGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCg                                                                                                                                            >  5:354162/1‑149 (MQ=255)
   aTGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCg                                                                                                                                            >  3:167009/1‑149 (MQ=255)
       gTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCgggg                                                                                                                                         >  6:328402/1‑148 (MQ=255)
       gTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCggg                                                                                                                                          <  1:20234/147‑1 (MQ=255)
       gTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGGTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCggg                                                                                                                                          >  2:20234/1‑147 (MQ=255)
        ttAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGa                                                                                                                                       <  3:40297/149‑1 (MQ=255)
        ttAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGa                                                                                                                                       <  8:314569/149‑1 (MQ=255)
            cccTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCgag                                                                                                                                                 >  6:249967/1‑135 (MQ=255)
            cccTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCgag                                                                                                                                                 <  5:249967/135‑1 (MQ=255)
            cccTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCTTCTCGCGGGGGAGttt                                                                                                                                   >  7:225360/1‑147 (MQ=255)
            cccTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCg                                                                                                                                   >  3:89006/1‑149 (MQ=255)
             ccTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCTTCTCGCGGGGGAGtt                                                                                                                                    >  7:245614/1‑146 (MQ=255)
             ccTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCTTCTCGCGGGGGAGtt                                                                                                                                    <  8:245614/147‑2 (MQ=255)
              cTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCTTCTCGCGGGGGAGttttc                                                                                                                                 <  8:225360/149‑5 (MQ=255)
                 cAGGCACCTGCGCAGCGTGTGACGCTGGAATTTAGCGGTTGCCCGATAGCCGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAACACTCCCCCGCGAGAAGCGGGGGAGTCGCTg                                                                                                                                <  8:284796/147‑1 (MQ=255)
                        cTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGt                                                                                                                       >  2:94201/1‑149 (MQ=255)
                         tGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGtt                                                                                                                      >  7:99763/1‑149 (MQ=255)
                         tGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGtt                                                                                                                      >  2:4912/1‑149 (MQ=255)
                           cgcAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGtttt                                                                                                                    <  7:16380/149‑1 (MQ=255)
                           cgcAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGttt                                                                                                                     <  1:397506/148‑1 (MQ=255)
                            gcAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGATGCTGATGGTTTCTGCCCGATGGATAAGTTTGTTAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCTCGAGAAGCGGGGGAGTCGCTGGTTAAAAGtttt                                                                                                                    >  5:313740/1‑148 (MQ=255)
                            gcAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGtttt                                                                                                                    >  1:309010/1‑148 (MQ=255)
                             cAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTa                                                                                                                  <  7:79503/149‑1 (MQ=255)
                                  tgtgACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTc                                                                                                             >  3:99593/1‑149 (MQ=255)
                                       cGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATgg                                                                                                        <  7:206319/149‑1 (MQ=255)
                                          tGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGttt                                                                                                                     <  8:104761/133‑1 (MQ=255)
                                          tGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGttt                                                                                                                     >  7:104761/1‑133 (MQ=255)
                                              aTTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTc                                                                                                 >  7:122900/1‑149 (MQ=255)
                                                 aaGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGc                                                                                               <  4:219923/148‑1 (MQ=255)
                                                      gTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGt                                                                                                   >  7:407729/1‑139 (MQ=255)
                                                      gTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGt                                                                                                   <  8:407729/139‑1 (MQ=255)
                                                          cccGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATg                                                                                                         <  3:59591/129‑1 (MQ=255)
                                                          cccGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATg                                                                                                         >  4:59591/1‑129 (MQ=255)
                                                          cccGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAg                                                                                     <  4:131422/149‑1 (MQ=255)
                                                          cccGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAg                                                                                     >  3:341970/1‑149 (MQ=255)
                                                           ccGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGc                                                                                    <  4:99593/149‑1 (MQ=255)
                                                            cGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGc                                                                                    >  3:39124/1‑148 (MQ=255)
                                                            cGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCg                                                                                   <  4:155809/149‑1 (MQ=255)
                                                                   cGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCttt                                                                            <  4:167009/149‑1 (MQ=255)
                                                                   cGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCttt                                                                            <  8:132318/149‑1 (MQ=255)
                                                                    gCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTa                                                                           <  8:122900/149‑1 (MQ=255)
                                                                     cTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTAt                                                                          <  1:94201/149‑1 (MQ=255)
                                                                        aTGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGCTGAATGAAGCGGTGACATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATc                                                                         <  1:317002/147‑1 (MQ=255)
                                                                          ggTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAg                                                                                     <  6:98402/133‑1 (MQ=255)
                                                                          ggTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAg                                                                                     >  5:98402/1‑133 (MQ=255)
                                                                           gTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCt                                                                    <  6:354162/149‑1 (MQ=255)
                                                                            tttCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGTGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCtt                                                                   <  2:3293/149‑1 (MQ=255)
                                                                                tGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCaaaaaa                                                                                      >  4:41356/1‑126 (MQ=255)
                                                                                tGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCaaaaaa                                                                                      <  3:41356/126‑1 (MQ=255)
                                                                                  cccGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCa                                                               >  3:263260/1‑147 (MQ=255)
                                                                                   ccGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTTAACGTTTTTACGTTAGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAg                                                            <  5:175600/149‑1 (MQ=255)
                                                                                    cGATGGATAAGTTTGATAGCGTGTTGAATGAAGCTGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAgg                                                           >  4:104139/1‑149 (MQ=255)
                                                                                         gATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCttt                                                       <  8:99763/148‑1 (MQ=255)
                                                                                          aTAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGa                                                     <  3:104139/149‑1 (MQ=255)
                                                                                          aTAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGa                                                     <  4:39124/149‑1 (MQ=255)
                                                                                             aGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACt                                                   >  3:260571/1‑148 (MQ=255)
                                                                                             aGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTa                                                  <  4:263260/149‑1 (MQ=255)
                                                                                              gTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTaa                                                 <  6:51887/149‑1 (MQ=255)
                                                                                                  gATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAacac                                              <  1:4912/148‑1 (MQ=255)
                                                                                                   aTAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAacac                                              >  6:110392/1‑147 (MQ=255)
                                                                                                   aTAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAacac                                              <  5:110392/147‑1 (MQ=255)
                                                                                                     aGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGc                                                                                               <  4:56507/96‑1 (MQ=255)
                                                                                                     aGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGc                                                                                               >  3:56507/1‑96 (MQ=255)
                                                                                                         tgtTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCAc                                      >  8:262334/1‑149 (MQ=255)
                                                                                                          gtTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCAc                                      >  3:266743/1‑148 (MQ=255)
                                                                                                          gtTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCAAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCAc                                      >  5:64246/1‑148 (MQ=255)
                                                                                                           ttGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTa                                    >  7:6515/1‑149 (MQ=255)
                                                                                                            tgaatgaaGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTAc                                   >  1:160532/1‑149 (MQ=255)
                                                                                                             gaatgaaGTGGTTAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGATGGTTAACCGTGTTAACCTTCGATGCTCGGTACGCCCCAAAAAAGCGAATCGTGATCGGTCTTAGCAAAGGCTTTGCCTAACACTGCATCACTAcc                                  >  6:216608/1‑149 (MQ=255)
                                                                                                              aatgaaGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACGCTTCATCACTACct                                 >  4:241629/1‑149 (MQ=255)
                                                                                                              aatgaaGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTACct                                 >  1:262456/1‑149 (MQ=255)
                                                                                                               atgaaGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAAt                                                                                <  2:320443/101‑1 (MQ=255)
                                                                                                                tgaaGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTACcttc                               <  2:262456/149‑1 (MQ=255)
                                                                                                                tgaaGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTACcttc                               <  5:328402/149‑1 (MQ=255)
                                                                                                                   aGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTACCttcttc                            <  2:309010/149‑1 (MQ=255)
                                                                                                                          aaaTAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGCCTTTGACTAACACTTCATCACTACCTACTACCCaaaac                     >  4:80916/1‑147 (MQ=255)
                                                                                                                               aCAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTACCTTCTTCCCAAATCTTTTc                <  4:341970/149‑1 (MQ=255)
                                                                                                                                 aGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTACCTTCTTCCCAAATCTTTTCCg              >  7:52600/1‑149 (MQ=255)
                                                                                                                                   aaaaCTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTACCTTCTTCCCAAATCTTTTCCGc             >  7:65849/1‑148 (MQ=255)
                                                                                                                                            ccGCGAGAAGCGGGGGAGTCGCTGGTTAACCGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTtcg   <  4:78960/149‑1 (MQ=255)
                                                                                                                                            ccGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTtcg   <  2:160452/149‑1 (MQ=255)
                                                                                                                                             cgcgAGAAGCGGGGGGGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTtcgt  >  5:388745/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
CCGATGCGTTAACCCTGCAGGCACCTGCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCGATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAACAGAAAACTCCCCCGCGAGAAGCGGGGGAGTCGCTGGTTAAACGTTTTTACGTTCGATGGTCTGTTCGCCCCAAAAAAGCGAATCTTTATCGGTCTTAGCAAAGGCTTTGACTAACACTTCATCACTACCTTCTTCCCAAATCTTTTCCGCCATTTTTTCGT  >  NC_000913/1066699‑1066988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: