Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,857,578 C→T 6.2% M64I (ATG→ATA)  ydjI ← putative aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,857,5780CT 6.2% 203.2 / 2.5 64M64I (ATG→ATA) ydjIputative aldolase
Reads supporting (aligned to +/- strand):  ref base C (23/37);  new base T (2/2);  total (25/39)
Fisher's exact test for biased strand distribution p-value = 6.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.26e-01

GAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTCGATAACGCCCATCAACATTTCTGCATTCCATACGTTAAAATGGGCAATTGCATAAT  >  NC_000913/1857430‑1857723
                                                                                                                                                    |                                                                                                                                                 
gAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACcatc                                                                                                                                                   <  5:68937/149‑1 (MQ=255)
gAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACcatc                                                                                                                                                   <  2:254359/149‑1 (MQ=255)
    atTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCAtgt                                                                                                                                               >  1:144666/1‑149 (MQ=255)
     ttttCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCAtgt                                                                                                                                               <  3:332110/148‑1 (MQ=255)
     ttttCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCAtgt                                                                                                                                               <  2:111089/148‑1 (MQ=255)
       ttcttcGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCTACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTga                                                                                                                                             <  1:55556/148‑1 (MQ=255)
       ttcttcGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTga                                                                                                                                             <  6:16924/148‑1 (MQ=255)
       ttcttcGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTga                                                                                                                                             <  1:282085/148‑1 (MQ=255)
         catcGAAATCAAACGCTGAAGCATCACGAATTAATGAATTCATACCATGAGTCCATGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCCGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTgaga                                                                                                                                           <  3:222306/146‑1 (MQ=255)
             gAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGaaa                                                                                                                                       <  6:146862/148‑1 (MQ=255)
             gAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAAt                                                                                                                                      <  2:369584/149‑1 (MQ=255)
                 tCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTc                                                                                                                                  <  4:153056/149‑1 (MQ=255)
                 tCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTc                                                                                                                                  <  1:4291/149‑1 (MQ=255)
                   aaaCGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACGATCATGTGAGAGAAATCTTCa                                                                                                                                 >  4:67513/1‑148 (MQ=255)
                   aaaCGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCaa                                                                                                                                <  7:138670/149‑1 (MQ=255)
                   aaaCGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCaa                                                                                                                                <  4:379136/149‑1 (MQ=255)
                   aaaCGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCa                                                                                                                                 >  1:49457/1‑148 (MQ=255)
                     aCGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAAt                                                                                                                              <  6:91857/149‑1 (MQ=255)
                      cgcgGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATg                                                                                                                             <  8:144403/149‑1 (MQ=255)
                        gtgAAGCATCACGTATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTGCATACTGCGACCATGATCCCAATGAGGTATTACCGGCACCGTTGCTTTTTGTGCAATTGATACCATCATGTGATATAAATCTTCAAATGAg                                                                                                                           <  4:272506/147‑1 (MQ=255)
                                tCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTAcc                                                                                                                   <  8:330971/149‑1 (MQ=255)
                                tCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTAcc                                                                                                                   >  4:24006/1‑149 (MQ=255)
                                  aCGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATTATGTGAGAGAAATCTTCAAATGAGGTGTTACCg                                                                                                                  >  2:402348/1‑148 (MQ=255)
                                  aCGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACAATTATGTGAGAGAAATCTTCAAATGAGGTGTTACCg                                                                                                                  >  2:396741/1‑148 (MQ=255)
                                        tAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTgagaga                                                                                                                                         >  1:77791/1‑119 (MQ=255)
                                        tAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTgagaga                                                                                                                                         <  2:77791/119‑1 (MQ=255)
                                          aTGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCt                                                                                                         >  7:277887/1‑149 (MQ=255)
                                            gAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTg                                                                                                        >  3:18583/1‑148 (MQ=255)
                                             aaTTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATTATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGt                                                                                                       <  1:402348/148‑1 (MQ=255)
                                             aaTTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATTATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGt                                                                                                       <  1:396741/148‑1 (MQ=255)
                                             aaTTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGt                                                                                                       <  8:311030/148‑1 (MQ=255)
                                              aTTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTAc                                                                                                     <  6:210791/149‑1 (MQ=255)
                                                  aTACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCCCCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTTTTACCGACAAAACCTGTACCaa                                                                                                  >  4:82505/1‑148 (MQ=255)
                                                   tACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAAc                                                                                                           <  2:236901/138‑1 (MQ=255)
                                                   tACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAAc                                                                                                           >  1:236901/1‑138 (MQ=255)
                                                   tACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCaaaa                                                                                                <  2:49457/149‑1 (MQ=255)
                                                      cATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGa                                                                                              >  2:128083/1‑148 (MQ=255)
                                                       aTGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGaaa                                                                                            <  8:310516/149‑1 (MQ=255)
                                                        tGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTGTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAaat                                                                                           <  4:26724/149‑1 (MQ=255)
                                                           gTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAaataa                                                                                         >  6:362160/1‑148 (MQ=255)
                                                                          ataatCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGc                                                                         >  7:135385/1‑149 (MQ=255)
                                                                             atCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGcttc                                                                      >  4:313189/1‑149 (MQ=255)
                                                                                  cATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCttcttc                                                                   <  1:128083/147‑1 (MQ=255)
                                                                                  cATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCttcttc                                                                   <  2:144666/147‑1 (MQ=255)
                                                                                  cATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCgg                                                                 >  3:190513/1‑149 (MQ=255)
                                                                                       tCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCg                                                            >  7:241924/1‑149 (MQ=255)
                                                                                         cGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTc                                                          <  8:135385/149‑1 (MQ=255)
                                                                                          gACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTc                                                          >  2:226973/1‑148 (MQ=255)
                                                                                              aTGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTCGATaa                                                     <  8:314681/149‑1 (MQ=255)
                                                                                                  tCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTCGATAACGcc                                                 >  3:291784/1‑149 (MQ=255)
                                                                                                   cccAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTCGATAACGcc                                                 >  6:84107/1‑148 (MQ=255)
                                                                                                     cAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTCGATAACGCCCAt                                              <  3:313189/149‑1 (MQ=255)
                                                                                                          aGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTCGATAACGCCCATCAAc                                          <  4:291784/148‑1 (MQ=255)
                                                                                                          aGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTCGATAACGCCCATCAAc                                          <  4:18583/148‑1 (MQ=255)
                                                                                                               ttaCCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTCGATAACGCCCATCAACATTTCt                                    <  1:210689/149‑1 (MQ=255)
                                                                                                                 aCCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTCGATAACGCCCATCAACATTTCTg                                   >  1:3782/1‑148 (MQ=255)
                                                                                                                   cGGCACCGTTGCTTTTTGTGCCATAGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTCGATAACGCCCATCAACATTTCTGCAt                                >  1:391456/1‑149 (MQ=255)
                                                                                                                         cGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTCGATACCGCCCATCAACATTTCTGCATTCCATa                          >  7:307443/1‑149 (MQ=255)
                                                                                                                             gCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTCGATAACGCCCATCAACATTTCTGCATTCCATAc                         <  6:364386/146‑1 (MQ=255)
                                                                                                                             gCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTCGATAACGCCCATCAACATTTCTGCATTCCATAc                         >  5:364386/1‑146 (MQ=255)
                                                                                                                              cTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGACGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTCGATAACGCCCATCAACATTTCTGCATTCCATACGtt                      <  2:365980/148‑1 (MQ=255)
                                                                                                                               tttttGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTCGATAACGCCCATCAACATTTCTGCATTCCATCCGTTa                     >  1:360426/1‑148 (MQ=255)
                                                                                                                                    gtgCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTCGATAACGCCCATCAACATTTCTGCATTCCATACGTTAAAATg                <  5:362160/148‑1 (MQ=255)
                                                                                                                                     tgCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTCGATAACGCCCATCAACATTTCTGCATTCCATACGTTAAAATgg               <  8:241924/148‑1 (MQ=255)
                                                                                                                                              tACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTCGATAACGCCCATCAACATTTCTGCATTCCATACGTTAAAATGGGCAATTGCAt     <  5:84107/149‑1 (MQ=255)
                                                                                                                                                ccATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTCGATAACGCCCATCAACATTTCTGCATTCCATACGTTAAAATGGGCAATTGCATaa   <  3:332019/149‑1 (MQ=255)
                                                                                                                                                 catcatGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTCGATAACGCCCATCAACATTTCTGCATTCCATACGTTAAAATGGGCAATTGCATAAt  >  4:299679/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GAATATTTTCTTCGAAATCAAACGCGGAAGCATCACGCATTAATGAATTCATACCATGAGTCCAGGCGTTATGAATAATCTCCATACTCCGACCATGATCCCAATGAGTTATTACCGGCACCGTTGCTTTTTGTGCCATTGATACCATCATGTGAGAGAAATCTTCAAATGAGGTGTTACCGACAAAACCTGTACCAAAAGAAATAATAACCGGGGATTTCGCTTCTTCGGCTGCGTCGATAACGCCCATCAACATTTCTGCATTCCATACGTTAAAATGGGCAATTGCATAAT  >  NC_000913/1857430‑1857723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑