| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,898,845 | G→A | 5.6% | R140H (CGT→CAT) | yoaD → | putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase, regulator of cellulose production |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,898,845 | 0 | G | A | 5.6% | 226.9 / 2.5 | 71 | R140H (CGT→CAT) | yoaD | putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase, regulator of cellulose production |
| Reads supporting (aligned to +/- strand): ref base G (36/31); new base A (2/2); total (38/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01 | |||||||||||
CAAAACTCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACGGCACACTTTATTGCTCCAGCATTTTTGGTTATCGCAATGTGCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTGGGGTCATGGAGATGATTAACATCGACTTACTGACGGCAATGCTGCTTGAGCCACAACTGCCGCAAATCAGTAGCGCCAGC > NC_000913/1898698‑1898990 | cAAAACTCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACGGCACACTTTATTGCTCCAGCATTTTTGGTTATCGCAATGTGCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGt > 4:267744/1‑149 (MQ=255)cAAAACTCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACGGCACACTTTATTGCTCCAGCATTTTTGGTTATCGCAATGTGCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGt < 3:230749/149‑1 (MQ=255) aaCTCCAAACTGGGCGATCCATTTGCCTGGTGCAATACGGAACACTTTATTGCTCCAGCATTTTTGGTTATCGCAATCTGCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTccc < 3:238278/149‑1 (MQ=255) cTCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACGGCACACTTTATTGCTCCAGCATTTTTGGTTATCGCAATGTGCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCt < 3:352571/149‑1 (MQ=255) tCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACGGCACACTTTATTGCTCCAGCATTTTTGGTTATCGCAATGTGCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGCTCGACCGTGCCCt > 6:244794/1‑148 (MQ=255) tCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACGGCACACTTTATTGCTCCAGCATTTTTGGTTATCGCAATGTGCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCt > 7:82623/1‑148 (MQ=255) cAAACTGTGCGATCCATTGGCCTGGTGCAAGACGGCACACTTTATTGCTCCAGCATTTTTGGTTATCGCAATGTGCCCGTCGTGGCCATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTCACGATCGACCGTGCCCTGAt > 5:205960/1‑149 (MQ=255) cTGTGCGATCCATTGTCCTGGTGCAAGACGGCACACTTTATTGCTCCAGCATTTTTGGTTATCGCAATGTGCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTaaa < 5:98945/149‑1 (MQ=255) cTGTGCGATCCATTGGCCTGGGGCAAGACGGCACACTTTATTGCTCCAGCATTTTTGCTTATCGCAATGTGCCCGTCGTGGACATTCTGGCGGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGAGCGACCGTGCCCTGATTaaa < 1:324383/149‑1 (MQ=255) gATCCATTGGCCTGGTGCAAGACGGCACACTTTATTGCTCCAGCATTTTTGGTTATCGCAATGTGCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGt > 5:162460/1‑149 (MQ=255) aTCCATTGGCCTGGTGCAAGACGGCACACTTTATTGCTCCAGCATTTTTGGTTATCGCAATGTGCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTc > 7:173648/1‑149 (MQ=255) tCCATTGGCCTGGTGCAAGACGGCACACTTTATTGCTCCAGCATTTTTGGTTATCGCAATGTGCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTcc > 7:180874/1‑149 (MQ=255) ttGGCCTGGTGCAAGACGGCACACTTTATTGCTCCAGCATTTTTGGTTATCGCAATGTGCCCGTCGTGGACATTCGGGCTGCACTTCCTGCACCGCAACCCCTTTTACGCCTGACGATCGACCGTGCCCGGATTAAAGGCAGTCCGGtg > 5:309741/1‑148 (MQ=255) gCCTGGTGCAAGACGGCACACTTTATTGCTCCAGCATTTTTGGTTATCGCAATGTGCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGtttt > 8:62261/1‑148 (MQ=255) tGGTGCAAGACGGCACACTTTATTGCTCCAGCATTTTTGGTTATCGCAATGTGCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCATGCCCTGATTAAAGGCAGTCCGGTTTTGAtt > 8:215117/1‑149 (MQ=255) gCAAGACGGCACACTTTATTGCTCCAGCATTTTTGGTTATCGCAATGTGCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAAt > 4:192162/1‑149 (MQ=255) cAAGACGGCACACTTTATTGCTCCAGCATTTTTGGTTATCGCAATGTGCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAAt < 8:82623/148‑1 (MQ=255) gACGGCACACTTTATTGCTCCAGCATTTTTGGTTATCGCAATGTGCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGAc > 7:287917/1‑149 (MQ=255) gACGGCACACTTTATTGCTCCAGCATTTTTGGTTATCGCAATGTGCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGAc > 6:288065/1‑149 (MQ=255) gACGGCACACTTTATTGCTCCAGCATTTTTGGTTATCGCAATGTGCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCATGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGAc > 5:84934/1‑149 (MQ=255) tATTGCTCCAGCATTTTTGGTTATCGCAATGTGCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCgg < 6:162460/148‑1 (MQ=255) aTTGCTCCAGCATTTTTGGTTATCGCAATGTGCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCggg > 5:74988/1‑148 (MQ=255) ttGCTCCAGCATTTTTGGTTATCGCAATGTGCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCa < 8:180874/149‑1 (MQ=255) cAGCATTTTTGGTTATCGCAATGTGCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGAt > 4:116291/1‑120 (MQ=255) cAGCATTTTTGGTTATCGCAATGTGCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGAt < 3:116291/120‑1 (MQ=255) aGCATTTTTGGTTATCGCAATGTGCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGttt < 6:36760/115‑1 (MQ=255) aGCATTTTTGGTTATCGCAATGTGCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGttt > 5:36760/1‑115 (MQ=255) aGCATTTTTGGTTATCGCAATGTGCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCaa > 4:337871/1‑149 (MQ=255) tttttGGTTATCGCAATGTGCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCt > 5:163860/1‑149 (MQ=255) tGGTTATCGCAATGTGCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTggg < 8:287917/148‑1 (MQ=255) tGGTTATCGCAATGTGCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTggg < 8:173648/148‑1 (MQ=255) tGGGTATCGCAATGTGCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGAGCGTGCCCTGATTAAACGCAGTACGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCTATGCTggg < 7:190157/148‑1 (MQ=255) tCGCAATGTGCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTGGGGTCATg > 1:325966/1‑148 (MQ=255) cAATGTGCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCACGACCACTTCTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTGGGGTCATg > 8:62141/1‑145 (MQ=255) cAATGTGCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCACGACCACTTCTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTGGGGTCATg < 7:62141/145‑1 (MQ=255) tgtgCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTGGGGTCATGGAgatga < 4:105868/149‑1 (MQ=255) tgCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTGGGGTCATGGAgatgat < 5:288065/148‑1 (MQ=255) gCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTGGGGTCATGGAGATGATTa < 6:302630/149‑1 (MQ=255) cccGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTGGGGTCATGGAGATGATTa < 2:325966/148‑1 (MQ=255) ccGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTGGGGTCATGGAGATGATTAAc < 7:214342/149‑1 (MQ=255) gtcgtGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTGGGGTCATGGAGATGATTAACAt < 2:95243/149‑1 (MQ=255) aCATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCATGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTGGGGTCATGGAGATGATTAACATCGACTTa < 7:215117/149‑1 (MQ=255) aTTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTGGGGTCATGGAGATGATTAACATCGACTTACt > 1:389614/1‑149 (MQ=255) gCTGAACTTCCTGCACCGCAACGACTTTTACGCCTGCCGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTGGATTCAATGGACGCCTGCAGCGTCCAGGAGCTATGATTGGCTCATGGAGATCATAAACATCGACTTACTGCCgg > 4:278217/1‑148 (MQ=255) gCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTGGGGTCATGGAGATGATTAACATCGACTTACTGAc < 6:163860/146‑1 (MQ=255) gCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTGGGGTCATGGAGATGATTAACATCGACTTACTGACgg < 3:337871/148‑1 (MQ=255) gCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTGGGGTCATGGAGATGATTAACATCGACTTACTGACgg > 7:34993/1‑148 (MQ=255) tGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTGGGGTCATGGAGATGATTAACATCGAc < 1:43792/136‑1 (MQ=255) tGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTGGGGTCATGGAGATGATTAACATCGAc > 2:43792/1‑136 (MQ=255) aaCTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTGGGGTCATGGAGATGATTAACATCGACTTa < 5:252214/137‑1 (MQ=255) aaCTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTGGGGTCATGGAGATGATTAACATCGACTTa > 6:252214/1‑137 (MQ=255) cTGCACCGCAACCACTTTTACGCAGGAAGATCGACCGCGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCGGGGGCCATGGAGATGATTAACATCGACTTTCTGACGGCAATGCTGCtt < 4:217698/149‑1 (MQ=255) tGCACCGCAACCTCTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTGAATGGACGCCTGCAGCggg < 8:336524/87‑1 (MQ=255) tGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCggg > 7:336524/1‑87 (MQ=255) cACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTGGGGTCATGGAGATGATTAACATCGACTTACTGACGGTAATGCTGCTTGAg > 1:357069/1‑149 (MQ=255) cACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTGGGGTCATGGAGATGATTAACATCGACTTACTGACGGCAATGCTGCTTGa < 8:125710/148‑1 (MQ=255) ttACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTGGGGTCATGGAGATGATTAACATCGACTTACTGACGGCAATGCTGCTTGAGCCACAACTGCCGc > 2:18654/1‑148 (MQ=255) aCGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGGTTTGATTCAAAGGACGCCTGCAGCGGGCAGTAGCAATGCTGGGGACATGGAGATGATTAACATCGACTTACTGACGGCAATGCTGCTTGAGCCACAACTGCCGCaaa > 6:105161/1‑149 (MQ=255) ccTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTGGGGTCATGGAGATGATTAACATCGACTTACTGACGGCAATGCTGCTTGAGCCACAACTGCCGCAAATCa < 3:192162/149‑1 (MQ=255) cTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTGGGGTCATGGAGATGATTAACATCGACTTACTGACGGCAATGCTGCTTGCGCCACAACTGCCGCAAATCAg > 7:75545/1‑149 (MQ=255) cTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTGGGGTCATGGAGATGATTAACATCGACTTACTGACGGCAATGCTGCTTGAGCCACAACTGCCGCAAATCAg > 5:388783/1‑149 (MQ=255) cTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTGGGGTCATGGAGATGATTAACATCGACTTACTGACGGCAATGCTGCTTGAGCCACAACTGCCGCAAATCAg > 7:181224/1‑149 (MQ=255) gACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTGGGGTCATGGAGATGATTAACATCGACTTACTGACGGCAATGCTGCTTGAGCCACAACTGCCGCAAATCAg > 8:50684/1‑147 (MQ=255) cgttCGACCGTGCCCTGATTAAAGGCAGGCCTGGTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTGGGGGCATGGAGATGATTAACATCGACTTACTGACGGCAATGCTGCTTGAGCCACAACTGCCGCAAATCAGTAgc < 6:309741/146‑1 (MQ=255) cGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTGGGGTCATGGAGATGATTAACATCGACTTACTGACGGCAATGCTGCTTGAGCCACAACTGCCGCAAATCAGTAgc < 8:181224/149‑1 (MQ=255) gATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTGGGGTCATGGAGATGATTATCATCGACTTACTGACGGCAATGCTGCTTGAGCCACAACTGCCGCAAATCAGTAgcg > 7:131352/1‑149 (MQ=255) gATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTGGGGTCATGGAGATGATTAACATCGACTTACTGACGGCAATGCTGCTTGAGCCACAACTGCCGCAAATCAGTAgcg > 7:128575/1‑149 (MQ=255) aTCGACCATGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTGGGGTCTTGGAGATGATTAACATCGACTTACTGACGGCAATGCTGCTTGAGCCACAACTGCCGCAAATCAGTAgcgc < 6:84934/149‑1 (MQ=255) gACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTGGGGTCATGGAGATGATTAACATCGACTTACTGACGGCAATGCTGCTTGAGCCACAACTGCCGCAAATCAGTAGCGCCAg > 8:31332/1‑149 (MQ=255) aCCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTGGGGTCATGGAGATGATTAACATCGACTTACTGACGGCAATGCTGCTTGAGCCACAACTGCCGCAAATCAGTAGCGCCAGc < 3:267744/149‑1 (MQ=255) aCCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTGGGGTCATGGAGATGATTAACATCGACTTACTGACGGCAATGCTGCTTGAGCCACAACTGCCGCAAATCAGTAGCGCCAGc > 4:109928/1‑149 (MQ=255) | CAAAACTCCAAACTGTGCGATCCATTGGCCTGGTGCAAGACGGCACACTTTATTGCTCCAGCATTTTTGGTTATCGCAATGTGCCCGTCGTGGACATTCTGGCTGAACTTCCTGCACCGCAACCACTTTTACGCCTGACGATCGACCGTGCCCTGATTAAAGGCAGTCCGGTTTTGATTCAATGGACGCCTGCAGCGGGCAGTAGCAATGCTGGGGTCATGGAGATGATTAACATCGACTTACTGACGGCAATGCTGCTTGAGCCACAACTGCCGCAAATCAGTAGCGCCAGC > NC_000913/1898698‑1898990 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |