Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,900,361 C→T 7.0% V409I (GTC→ATC)  yoaE ← putative membrane protein/conserved protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,900,3610CT 7.0% 168.9 / 3.1 57V409I (GTC→ATC) yoaEputative membrane protein/conserved protein
Reads supporting (aligned to +/- strand):  ref base C (28/25);  new base T (2/2);  total (30/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.95e-01

TGCTCAACATCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACG  >  NC_000913/1900216‑1900506
                                                                                                                                                 |                                                                                                                                                 
tGCTCAACATCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCTCCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAg                                                                                                                                                <  8:302394/149‑1 (MQ=255)
  cTCAAAATCAAGCGCCTGCTCCAAGGCATGCAAATCTGGACCGCCTTATACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTACCAGAACATCCAGCCCCGTGCCCAGAc                                                                                                                                              >  6:316363/1‑149 (MQ=255)
         tCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTAc                                                                                                                                       <  1:171224/149‑1 (MQ=255)
           aaGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGAc                                                                                                                                                   >  1:84953/1‑135 (MQ=255)
           aaGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGAc                                                                                                                                                   <  2:84953/135‑1 (MQ=255)
             gcgcCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACcaca                                                                                                                                   >  6:306982/1‑149 (MQ=255)
             gcgcCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACcaca                                                                                                                                   <  7:96304/149‑1 (MQ=255)
                ccTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGAc                                                                                                                                              <  4:139258/135‑1 (MQ=255)
                ccTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGAc                                                                                                                                              >  3:139258/1‑135 (MQ=255)
                       cAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGt                                                                                                                         <  1:28920/149‑1 (MQ=255)
                        aaGGCATGCAACTCTGTACCGCCTTTTACCATCCAGCCGTCACCAGCAGTAATGATTTCCGGCGTGTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCCGACCCGTCACAAGACCTAGTACCAAACCAAACTCGtt                                                                                                                        >  4:61243/1‑149 (MQ=255)
                         aGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTg                                                                                                                       <  3:350540/149‑1 (MQ=255)
                          ggCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGGCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTgg                                                                                                                      >  6:109828/1‑149 (MQ=255)
                            cATGCAATTCTGTACCGCCTTTTACGCGCCAGCCGGAACCATCAGTAATGATTTGCGTCGGTTCGTCAGCGTGCTGTAATTAAACCGCAATGGCTTCCATCACATCAAGCGGCGTGACCAGACCTTGTAAAACAAAATACTCGTTGGTc                                                                                                                    <  8:405698/149‑1 (MQ=255)
                              tGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCAc                                                                                                                  <  1:282752/149‑1 (MQ=255)
                               gCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGt                                                                                                                                         >  1:136992/1‑125 (MQ=255)
                               gCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGt                                                                                                                                         <  2:136992/125‑1 (MQ=255)
                               gCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACACGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTAACACACCAAACTCGTTGGTCAc                                                                                                                  >  5:189006/1‑148 (MQ=255)
                                  aaTCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGAt                                                                                                               >  8:26579/1‑148 (MQ=255)
                                        tACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAg                                                                                                        >  3:241530/1‑149 (MQ=255)
                                          ccGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCt                                                                                                      >  8:38084/1‑149 (MQ=255)
                                             ccTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTccc                                                                                                   <  7:319399/149‑1 (MQ=255)
                                               ttttACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGt                                                                                                                                         >  4:174990/1‑109 (MQ=255)
                                               ttttACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGt                                                                                                                                         <  3:174990/109‑1 (MQ=255)
                                                  tACCAGCCAGCCGTCACAAGCAGTAATGATTTCCGGCGTTGCGGCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCAAGCGGCGTGACCAGACCTTGTACCACACCAATCTCGTTGGTCACGATAACAACGCTCCCGCGa                                                                                               <  3:61243/148‑1 (MQ=255)
                                                      agccagccGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGt                                                                                                                                         >  2:19952/1‑102 (MQ=255)
                                                      agccagccGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGt                                                                                                                                         <  1:19952/102‑1 (MQ=255)
                                                       gccagccGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTc                                                                                                     <  5:195730/137‑1 (MQ=255)
                                                       gccagccGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTc                                                                                                     >  6:195730/1‑137 (MQ=255)
                                                           gccGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCTGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCacgacg                                                                                       >  8:148592/1‑147 (MQ=255)
                                                               tCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCACGACGCAGCAc                                                                                 >  5:377310/1‑149 (MQ=255)
                                                               tCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAAATCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCACGACGCAGCAc                                                                                 <  5:109828/149‑1 (MQ=255)
                                                                cACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGATCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACg                                                                                >  2:248344/1‑149 (MQ=255)
                                                                cACCATCAGTAATGATTTCCGGCGGTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGATCAGACCTTGTACCACACCAAACTCGGTGGTCACGATAACAAAGCTCCCGCGAGCCCGACGCAGCCCg                                                                                >  2:242558/1‑149 (MQ=255)
                                                                 aCCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGc                                                                               <  4:241530/149‑1 (MQ=255)
                                                                 aCCATCAGTAATGATTTCCGGCGGTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACg                                                                                <  1:299321/148‑1 (MQ=255)
                                                                         tAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGAGACCAGACCTTGTACCACACCAAACTCGTTGGACACGATAACAAATCTCCCGCCAGCACGACGCAGCACGCCCAACAgc                                                                       >  2:237391/1‑148 (MQ=255)
                                                                           aTGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGt                                                                      <  5:306982/148‑1 (MQ=255)
                                                                               tttCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACAAAACTCGTTGGACACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTgatc                                                                 >  7:339504/1‑149 (MQ=255)
                                                                                       gTTTCGTGAGCGTCCGGGAATTCACCCGCAAAGGCTTCCAGCACATCCAGCGGCGGGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAAGATAGCTCCAGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAg                                                         <  5:316363/149‑1 (MQ=255)
                                                                                                   tCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGCCCTTGTACCACCCCAAACTCGTTGGGCACGATAACAAAGCTCCCGCGAGCACGCCGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCGGGAc                                             >  4:202650/1‑149 (MQ=255)
                                                                                                      gggAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTgat                                                                  <  5:287497/125‑1 (MQ=255)
                                                                                                        gAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCGGGACGATaa                                        <  7:38084/149‑1 (MQ=255)
                                                                                                            tCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTc                                                   <  1:173825/134‑1 (MQ=255)
                                                                                                            tCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTc                                                   >  2:173825/1‑134 (MQ=255)
                                                                                                            tCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATCGc                                    >  7:72200/1‑149 (MQ=255)
                                                                                                              ccccGAAATTTCTTCAAGCACATAAAGCTGCTTGACCAGACCTTGTACCACAAAAAACTCGTGGGTCACGATAACAAAGCTCAAGCGCTCCCGAAGCATCACGCCCAAAAGTTTGATCTGATCGAGGTTTTCCGGGACGAGAATCTCCg                                  >  4:113613/2‑149 (MQ=255)
                                                                                                              aCCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATCGCCg                                  >  1:37397/1‑149 (MQ=255)
                                                                                                                ccGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATCGCCGga                                >  4:268533/1‑149 (MQ=255)
                                                                                                                      tGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACAAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATCGCCggctccgcc                          >  2:374010/1‑142 (MQ=255)
                                                                                                                            ccAGCACATCCAGCGGCGTGATCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTGGATCGGATCGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAg                      <  1:248344/147‑1 (MQ=255)
                                                                                                                            ccAGCACATCCAGCGGCGGGATCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAg                      <  1:242558/147‑1 (MQ=255)
                                                                                                                                   aTCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCTGGACGATAATCGCCGGAGACGCCGAAGCAATcgccg             >  5:363617/1‑149 (MQ=255)
                                                                                                                                   aTCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATcgccg             >  7:104572/1‑149 (MQ=255)
                                                                                                                                    tCCAGCGGCGTGACCAGACCTTGTACCACACGAAACTCGTTTGTCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGCTCGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATcgccgc            <  6:189006/149‑1 (MQ=255)
                                                                                                                                      cAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCca          <  6:377310/149‑1 (MQ=255)
                                                                                                                                       aGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCca          >  1:15843/1‑148 (MQ=255)
                                                                                                                                             gTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAAc   <  7:26579/149‑1 (MQ=255)
                                                                                                                                              tGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACg  >  1:350974/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
TGCTCAACATCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGTTGATCGGATCGAGGGTTTCCGGGACGATAATCGCCGGAGACGCCGAAGCAATCGCCGCCACATCAACG  >  NC_000913/1900216‑1900506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑