Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,934,587 T→C 62.9% L6L (TTA→TTG edd ← 6‑phosphogluconate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,934,5870TC62.9% 66.4 / 65.4 70L6L (TTA→TTGedd6‑phosphogluconate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (7/19);  new base C (18/26);  total (25/45)
Fisher's exact test for biased strand distribution p-value = 3.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGGCAGGCAGCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAA  >  NC_000913/1934445‑1934732
                                                                                                                                              |                                                                                                                                                 
gctggcAGGCAGCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAAc                                                                                                                                                <  4:147033/146‑1 (MQ=255)
gctggcAGGCAGCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAAc                                                                                                                                                <  6:193003/146‑1 (MQ=255)
    gcaggcagCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATtgt                                                                                                                                          <  7:118697/148‑1 (MQ=255)
    gcaggcagCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATtgt                                                                                                                                          <  7:125672/148‑1 (MQ=255)
      aggcagCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGa                                                                                                                                       >  1:364826/1‑149 (MQ=255)
          agCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCa                                                                                                                                   <  2:109562/149‑1 (MQ=255)
             gAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATaa                                                                                                                                <  4:188548/149‑1 (MQ=255)
             gAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATa                                                                                                                                 >  4:25488/1‑148 (MQ=255)
               aaCCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAg                                                                                                                              >  6:143454/1‑149 (MQ=255)
                   gtgtGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTc                                                                                                                          <  1:270467/149‑1 (MQ=255)
                            gTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATCCATAAAGGCTCCTGAAAtt                                                                                                                  >  6:378207/1‑148 (MQ=255)
                               aCCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAg                                                                                                               <  5:276753/148‑1 (MQ=255)
                               aCCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAg                                                                                                               <  2:152798/148‑1 (MQ=255)
                               aCCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAg                                                                                                               <  5:331583/148‑1 (MQ=255)
                                  gCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGt                                                                                                           >  8:16752/1‑149 (MQ=255)
                                    aTGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCa                                                                                                         <  5:49990/149‑1 (MQ=255)
                                    aTGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCa                                                                                                         <  4:291382/149‑1 (MQ=255)
                                    aTGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAgg                                                                                                                             >  5:107959/1‑129 (MQ=255)
                                    aTGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAgg                                                                                                                             <  6:107959/129‑1 (MQ=255)
                                       ccAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTTTCCGGGCGAGATAAGCAGAGCGAGTCTCTCGCGAACGTTCTATGTTTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCaga                                                                                                       <  1:195392/148‑1 (MQ=255)
                                        cAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTc                                                                                                                                    >  8:156136/1‑118 (MQ=255)
                                         aaCTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGGGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCa                                                                                                    <  2:372299/149‑1 (MQ=255)
                                              cGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGgatg                                                                                               <  1:400162/149‑1 (MQ=255)
                                               gAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGgatga                                                                                              >  7:314683/1‑149 (MQ=255)
                                               gAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGgatga                                                                                              <  3:25488/149‑1 (MQ=255)
                                                   gATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTca                                                                                          >  7:308304/1‑149 (MQ=255)
                                                   gATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTca                                                                                          >  2:163126/1‑149 (MQ=255)
                                                       aaCGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATGCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACaa                                                                                       <  5:378207/148‑1 (MQ=255)
                                                           gTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGAt                                                                                                                                      >  8:341048/1‑97 (MQ=255)
                                                           gTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGAt                                                                                                                                      <  7:341048/97‑1 (MQ=255)
                                                              gAAGTTTTCGCTTGTTCTTTCCGGTCGAGATAAGCAGAGCGAGTCTCGATCGAACGTTCAATGATTCGATTTGTTACGCGTAAAAATTGTGGATTCATAAAGTCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTttc                                                                               <  1:368383/149‑1 (MQ=255)
                                                              gAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGttt                                                                                >  2:135075/1‑148 (MQ=255)
                                                                aGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACCCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTttcat                                                                             <  3:220952/149‑1 (MQ=255)
                                                                    ttCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTttcattca                                                                          <  7:146725/148‑1 (MQ=255)
                                                                      cGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCaga                                                                        <  8:339550/148‑1 (MQ=255)
                                                                       gCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTttcattca                                                                          <  4:94799/145‑1 (MQ=255)
                                                                       gCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTttcattca                                                                          >  3:94799/1‑145 (MQ=255)
                                                                        cTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGa                                                                     <  6:161492/149‑1 (MQ=255)
                                                                           gTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGAtt                                                                   >  6:183641/1‑148 (MQ=255)
                                                                            ttCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGAttt                                                                  <  8:314683/148‑1 (MQ=255)
                                                                            ttCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGAttt                                                                  <  7:16752/148‑1 (MQ=255)
                                                                                 tCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACt                                                            <  2:51250/149‑1 (MQ=255)
                                                                                 tCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACt                                                            <  5:143454/149‑1 (MQ=255)
                                                                                 tCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGa                                                              <  8:134016/147‑1 (MQ=255)
                                                                                 tCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACt                                                            >  8:391076/1‑149 (MQ=255)
                                                                                   cGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGa                                                          <  4:327594/149‑1 (MQ=255)
                                                                                          gatattcAGAGGGAGTCTCGCGCGAAAGTTCAATGATTCGATTTTTTACGCTTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACTCGTTTCATTCAGAGGATTTATGACTTAAACGCCt                                                   <  4:334516/143‑1 (MQ=255)
                                                                                          gaTAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCt                                                   <  7:391076/149‑1 (MQ=255)
                                                                                           aTAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTg                                                  >  3:54125/1‑149 (MQ=255)
                                                                                            tAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCTATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTg                                                  <  8:115471/148‑1 (MQ=255)
                                                                                              aGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTaa                                               <  5:183641/149‑1 (MQ=255)
                                                                                                 agagCGAGTCTCGCGCGCACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCg                                            >  1:134299/1‑149 (MQ=255)
                                                                                                           tcGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAg                                  >  5:272484/1‑149 (MQ=255)
                                                                                                             gcgcgAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATa                                   <  1:163126/146‑1 (MQ=255)
                                                                                                             gcgcgAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAggg                                >  3:120833/1‑149 (MQ=255)
                                                                                                              cgcgAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGc                               <  5:139620/149‑1 (MQ=255)
                                                                                                                         aaTGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACTCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGttt                     >  1:312065/1‑148 (MQ=255)
                                                                                                                         aaTGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTAAGAGCAGGATGATTCATAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGttt                     >  6:214360/1‑148 (MQ=255)
                                                                                                                          aTGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGttt                     >  2:306190/1‑147 (MQ=255)
                                                                                                                             aTTCGCTTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTTAAACGCCTGTAACCGGAGCTGATAGGGCAAAAAGGTTtcagt                 >  1:306961/1‑148 (MQ=255)
                                                                                                                             aTTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTtcagt                 <  8:296542/148‑1 (MQ=255)
                                                                                                                              ttCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCagag                                                                       >  6:20589/1‑93 (MQ=255)
                                                                                                                              ttCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCagag                                                                       <  5:20589/93‑1 (MQ=255)
                                                                                                                              ttCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTtcagtca               <  4:120833/149‑1 (MQ=255)
                                                                                                                                cGATTTGTTACGCTCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTtcagtcag              <  2:364826/148‑1 (MQ=255)
                                                                                                                                 gATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAgtg            <  8:308304/149‑1 (MQ=255)
                                                                                                                                 gATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCGCATAGGGCAAAAACGTTTCAGTCAgtg            >  6:98689/1‑149 (MQ=255)
                                                                                                                                 gATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAACGTTTCAGTCAgtgt           <  2:134299/149‑1 (MQ=255)
                                                                                                                                      gTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGtaat        <  6:272484/148‑1 (MQ=255)
                                                                                                                                          cgcgCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTGAGAGCAGGATGATTCATAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATaaaa    <  5:214360/148‑1 (MQ=255)
                                                                                                                                           gcgcAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATaaaaaa  <  5:190717/149‑1 (MQ=255)
                                                                                                                                           gcgcAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATaaaaa   >  4:352234/1‑148 (MQ=255)
                                                                                                                                           gcgcAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATaaaaa   >  5:56237/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
GCTGGCAGGCAGCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAA  >  NC_000913/1934445‑1934732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: