Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,076,922 G→T 6.8% E36* (GAA→TAA)  yeeT → CP4‑44 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,076,9220GT 6.8% 226.0 / 5.8 74E36* (GAA→TAA) yeeTCP4‑44 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (38/31);  new base T (3/2);  total (41/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.60e-01

TTTTCACTTTTATCTTCTGTCTTCAGAGGTATCCCATTATGAAAATTATCACCCGTGGTGAAGCCATGCGTATTCACCAACAACATCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGTTTTGGTCCGTATGTCCGGCTGATGAGCGTCACCCTGAACTGAGTGGGAATTCTGATGAGCAGAAT  >  NC_000913/2076779‑2077061
                                                                                                                                               |                                                                                                                                           
ttttCACTTTTATCTTCTGTCTTCAGAGGTATCCCATTATGAAAATTATCACCCGTGGTGAAGCCATGCGTATTCACCAACAACATCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGc                                                                                                                                         <  7:31172/148‑1 (MQ=255)
     aCTTTTATCTTCTGTCTTCAGAGGTATCCCATTATGAAAATTATCACCCGTGGTGAAGCAATGCGTATTCACCAACAACATCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCCCGGCAGTGCTGAAGCGTATAc                                                                                                                                   >  5:285770/1‑149 (MQ=255)
       ttttATCTTCTGTCTTCAGAGGTATCCCATTATGAAAATTATCACCCGTGGTGAAGCCATGCGTATTCACCAACAACATCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCg                                                                                                                                 >  8:160563/1‑149 (MQ=255)
         ttATCTTCTGTCTTCAGAGGTATCCCATTATGAAAATTATCACCCGTGGTGAAGCCATGCGTATTCACCAACAACATCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGt                                                                                                                               >  8:74735/1‑149 (MQ=255)
              ttctGTCTTCAGAGGTATCCCATTATGAAAATTATCACCCGTGGTGAAGCCATGCGTATTCACCAACAACATCCGACATACCGTCTTTTTCCGTTCTGTACCGGTAAATACAGCTGGCACGGCAGTGCTGAAGCGTATACCGGACGTg                                                                                                                           >  7:368168/1‑148 (MQ=255)
                   tCTTCAGAGGTATCCCATTATGAAAATTATCACCCGTGGTGAAGCCATGCGTATTCACCAACAACATCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTg                                                                                                                      <  5:212692/148‑1 (MQ=255)
                   tCTTCAGAGGTATCCCATTATGAAAATTATCACCCGTGGTGAAGCCATGCGTATTCACCAACAACATCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTg                                                                                                                      >  1:383199/1‑148 (MQ=255)
                     ttCAGAGGTATCCCATTATGAAAATTATCACCCGTGGTGAAGCCATGCGTATTCACCAACAACATCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAg                                                                                                                   >  3:231174/1‑149 (MQ=255)
                       cAGAGGTATCCCATTATGAAAATTATCACCCGTGGTGAAGCCATGCGTATTCACCAACAACATCCGACATCCCGTCTTTTTCCTTTCTGTACCGTTAAATACCGCTGGCACGGCAGTGATGAAGCGAATACCGGACCTGAAGAGCAGGa                                                                                                                 >  6:48127/1‑149 (MQ=255)
                       cAGAGGTATCCCATTATGAAAATTATCACCCGTGGTGAAGCCATGCGTATTCACCAACAACATCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCa                                                                                                                    <  5:361291/146‑1 (MQ=255)
                       cAGAGGTATCCCATTATGAAAATTATCACCCGTGGTGAAGCCATGCGTATTCACCAACAACATCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAgg                                                                                                                  <  3:341698/148‑1 (MQ=255)
                          agGTATCCCATTATGAAAATTATCACCCGTGGTGAAGCCATGCGTATTCACCAACAACATCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAg                                                                                                                   >  4:272673/1‑144 (MQ=255)
                          agGTATCCCATTATGAAAATTATCACCCGTGGTGAAGCCATGCGTATTCACCAACAACATCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAg                                                                                                                   <  3:272673/144‑1 (MQ=255)
                             tATCCCATTATGAAAATTATCACCCGTGGTGAAGCCATGCGTATTCACCAACAACATCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGta                                                                                                                                      >  2:145139/1‑122 (MQ=255)
                             tATCCCATTATGAAAATTATCACCCGTGGTGAAGCCATGCGTATTCACCAACAACATCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGta                                                                                                                                      <  1:145139/122‑1 (MQ=255)
                                 ccATTATGAAAATTATCACCCGTGGTGAAGCCATGCGTATTCACCAACAACATCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGgt                                                                                                       >  7:56123/1‑149 (MQ=255)
                                         aaaaTTATCACCCGTGGTGAAGCCATGCGTATTCACCAACAACATCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCA‑TGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGcc                                                                                              >  4:303550/1‑149 (MQ=255)
                                           aaTTATCACCCGTGGTGAAGCCATGCGTATTCACCAACAACATCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCg                                                                                             >  5:193921/1‑149 (MQ=255)
                                                  aCCCGTGGTGAAGCCATGCGTATTCACCAACAACATCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTg                                                                                       <  7:74735/148‑1 (MQ=255)
                                                   cccGTGGTGAATCCATGAGTATTCCCCAACACCATCCGACATGGCTTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTTCTGAAGAGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCt                                                                                     <  3:112336/149‑1 (MQ=255)
                                                   cccGTGGTGAAGCCATGCGTATTCACCAACAACATCCGACATCCCGGCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCt                                                                                     <  3:322629/149‑1 (MQ=255)
                                                         gtgAAGCCATGCGTATTCCCCAACAACATCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGc                                                                               >  4:111405/1‑149 (MQ=255)
                                                            aaGCCATGCGTATTCACCAACAACATCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCg                                                                             <  4:231174/148‑1 (MQ=255)
                                                             aGCCATGCGTATTCACCAACAACATCAGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATAGCGCTGGCACGGCAGGGCGGATGCGTATACCGGTCGTGAAGTGCAGGATATTCGCGGTGTGCTGGGCGTGTTTGCTGAACGCCGt                                                                            <  5:48127/148‑1 (MQ=255)
                                                              gCCATGCGTATTCACCAACAACATCCGTCATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCAAGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTaa                                                                          <  3:284849/149‑1 (MQ=255)
                                                              gCCATGCGTATTCACCAACAACATCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTaa                                                                          <  6:40816/149‑1 (MQ=255)
                                                                 aTGCGTATTCACCAACAACATCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGa                                                                       >  5:137128/1‑149 (MQ=255)
                                                                  tGCGTATTCACCAACAACATCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGa                                                                       >  6:81906/1‑148 (MQ=255)
                                                                  tGCGTATTCACCAACAACATCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGa                                                                       >  6:87238/1‑148 (MQ=255)
                                                                     gTATTCACCAACAACATCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGt                                                                   <  2:383199/149‑1 (MQ=255)
                                                                     gTATTCACAAACAACATCCGACATACCGAATTTTTCCGTTCTGTACCGGTAAATACCGCTGTCACGGAATTGCTTAATCTTATACCGGTCGTGAAGTGCAGGATATTAACGTTGTGATGGCAGTGTTTGCTGAACGCCGTAAGGa                                                                       >  8:290844/1‑145 (MQ=255)
                                                                       aTTCACCAACAACATCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGTACAGttt                                                                 >  8:95332/1‑149 (MQ=255)
                                                                        ttCACCAACAACATCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGtttt                                                                <  1:175032/149‑1 (MQ=255)
                                                                           aCCAACAACATCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACa                                                                     >  1:137294/1‑141 (MQ=255)
                                                                           aCCAACAACATCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACa                                                                     <  2:137294/141‑1 (MQ=255)
                                                                             caacaaCATCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACTGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGTTTTgg                                                              <  8:123480/146‑1 (MQ=255)
                                                                             caacaaCATCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACAGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGTTTTgg                                                              >  7:123480/1‑146 (MQ=255)
                                                                               acaacaTCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGTTTTGGTCCg                                                          >  2:130747/1‑148 (MQ=255)
                                                                                caacaTCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGTTTTGGTCCGTa                                                        <  7:278443/149‑1 (MQ=255)
                                                                                    aTCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTTAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGCGTTTGCTGAACGCCGTAAGGACAGttt                                                                 >  1:340235/1‑136 (MQ=255)
                                                                                    aTCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTTAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGCGTTTGCTGAACGCCGTAAGGACAGttt                                                                 >  1:345500/1‑136 (MQ=255)
                                                                                    aTCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTTAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGCGTTTGCTGAACGCCGTAAGGACAGttt                                                                 <  2:345500/136‑1 (MQ=255)
                                                                                    aTCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTTAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGCGTTTGCTGAACGCCGTAAGGACAGttt                                                                 <  2:340235/136‑1 (MQ=255)
                                                                                     tCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGTTTTGGTCCGTATGTcc                                                   >  4:298279/1‑149 (MQ=255)
                                                                                     tCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGTTTTGGTCCGTATGTcc                                                   >  8:129997/1‑149 (MQ=255)
                                                                                        gACATCGCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGTTTTGGTCCGTATGTCCGGc                                                <  1:289531/149‑1 (MQ=255)
                                                                                           aTCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGTTTTGGTCCGTATGTCCGGCtga                                             <  7:129997/149‑1 (MQ=255)
                                                                                            tCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGTTTTGGTCCGTATGTCCGGCtgat                                            >  7:40255/1‑149 (MQ=255)
                                                                                             cccGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGTTTTGGTGCGTATGTCCGGCtgat                                            >  8:310485/1‑148 (MQ=255)
                                                                                              ccGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGTTTTGGTCCGTATGTCCGGCtgatga                                          <  6:137128/149‑1 (MQ=255)
                                                                                              ccGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGTTTTGGTCCGTATGTCCGGCtgat                                            >  8:199270/1‑147 (MQ=255)
                                                                                                gTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGTTTTGGTCCGTATGTCCGGCTGATGAGc                                        <  7:160563/149‑1 (MQ=255)
                                                                                                 tCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGTTTTGGTCCGTATGTCCGGCTGATGAGCg                                       >  2:186576/1‑149 (MQ=255)
                                                                                                   tttttCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGTTTTGGTCCGTATGTCCGGCTGATGAGCGt                                      >  5:89571/1‑148 (MQ=255)
                                                                                                   tttttCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGTTTTGGTCCGTATGTCCGGCTGATGAGCGt                                      >  7:339745/1‑148 (MQ=255)
                                                                                                     tttCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGTTTTGGTCCGAATGTCCGGCTGATGAGCGTCAc                                   >  7:238957/1‑149 (MQ=255)
                                                                                                          gTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGTTTTGGTCCGTATGTCCGGCTGATGAGCGTCACCCTg                               >  8:134881/1‑148 (MQ=255)
                                                                                                           ttCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGTTTTGGTCCGTATGTCCGGCTGATGAGCGTCACCCTGaa                             >  6:21910/1‑149 (MQ=255)
                                                                                                           ttCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGTTTTGGTCCGTATGTCCGGCTGATGAGCGTCACCCTGaa                             >  6:283635/1‑149 (MQ=255)
                                                                                                                   cGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGTTTTGGTCCGTATGTCCGGCTGATGAGCGTCACCCTGAACTGAGTgg                     >  6:346341/1‑149 (MQ=255)
                                                                                                                     gTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGTTTTGGTCCGTATGTCCGGCTGATGAGCGTCACCCTGAACTGAGTggg                    >  8:276041/1‑148 (MQ=255)
                                                                                                                         aTACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGTTTTGGTCCGTATGTCCGGCTGATGAGCGTCACCCTGAACTGAGTGGGAATTc               >  7:309444/1‑149 (MQ=255)
                                                                                                                            ccGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGTTTTGGTCCGTATGTCCGGCTGATGAGCGTCACCCTGAACTGAGTGGGAATTCtg             <  5:383226/148‑1 (MQ=255)
                                                                                                                             cGCTGGCACTGCAGTGCTGAAGCGTATACAGGTAGTGAAGTGCAGTATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGTTTTGGTCCGTATGTACTGCTGATGAGCGTCACCCTGAACTGAGTGGGAATTCtgat           <  7:290844/149‑1 (MQ=255)
                                                                                                                             cGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGTTTTGGTCCGTATGTCCGGCTGATGAGCGTCACCCTGAACTGAGTGGGAATTCtgat           <  3:298279/149‑1 (MQ=255)
                                                                                                                             cGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGTTTTGGTCCGTATGTCCGGCTGATGAGCGTCACCCTGAACTGAGTGGGAATTCtgat           <  8:40255/149‑1 (MQ=255)
                                                                                                                             cGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGTTTTGGTCCGTATGTCCGGCTGATGAGCGTCACCCTGAACTGAGTGGGAATTCtgat           <  1:186576/149‑1 (MQ=255)
                                                                                                                             cGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGTTTTGGTCCGTATGTCCGGCTGATGAGCGTCACCCTGAACTGAGTGGGAATTCtgat           <  2:396671/149‑1 (MQ=255)
                                                                                                                               cTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGTTTTGGTCCGTATGTCCGGCTGATGAGCGTCACCCTGAACTGAGTGGGAATTCtgatgc         >  8:330844/1‑148 (MQ=255)
                                                                                                                                  gCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGTTTTGGTCCGTATGTCCGGCTGATGAGCGTCACCCTGAACTGAGTGGGAATTCTGATGAGCa      <  6:89571/149‑1 (MQ=255)
                                                                                                                                     cGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGTTTTGGTCCGTTTGTCCGGCTTATGAGCGTCACCCTGAACTGAGTGGGAATTCTGATGAGCATaa   <  8:368168/149‑1 (MQ=255)
                                                                                                                                       gCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGTTTTGGTCCGTATGTCCGGCTGATGAGCGTCACCCTGAACTGAGTGGGAATTCTGATGAGCAGAAt  >  7:142335/1‑148 (MQ=255)
                                                                                                                                              tGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGTTTTGGTCCGTATGTCCGGCTGATGAGCGTCACCCTGAACTGAGTGGGAATTCTGATGAGCAGaa   <  3:331759/140‑1 (MQ=255)
                                                                                                                                              tGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGTTTTGGTCCGTATGTCCGGCTGATGAGCGTCACCCTGAACTGAGTGGGAATTCTGATGAGCAGaa   >  4:331759/1‑140 (MQ=255)
                                                                                                                                               |                                                                                                                                           
TTTTCACTTTTATCTTCTGTCTTCAGAGGTATCCCATTATGAAAATTATCACCCGTGGTGAAGCCATGCGTATTCACCAACAACATCCGACATCCCGTCTTTTTCCGTTCTGTACCGGTAAATACCGCTGGCACGGCAGTGCTGAAGCGTATACCGGTCGTGAAGTGCAGGATATTCCCGGTGTGCTGGCCGTGTTTGCTGAACGCCGTAAGGACAGTTTTGGTCCGTATGTCCGGCTGATGAGCGTCACCCTGAACTGAGTGGGAATTCTGATGAGCAGAAT  >  NC_000913/2076779‑2077061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑