Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 361,901 T→G 5.3% intergenic (‑41/+25) lacA ← / ← lacY thiogalactoside acetyltransferase/lactose permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913361,9010TG 5.3% 288.3 / 4.2 102intergenic (‑41/+25)lacA/lacYthiogalactoside acetyltransferase/lactose permease
Reads supporting (aligned to +/- strand):  ref base T (47/49);  new base G (2/4);  total (49/53)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

AACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGATAAGCGCCCTG  >  NC_000913/361754‑362045
                                                                                                                                                   |                                                                                                                                                
aaCTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTc                                                                                                                                                 >  7:273868/1‑149 (MQ=255)
 aCTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTcg                                                                                                                                                >  7:141550/1‑149 (MQ=255)
  cTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTcgc                                                                                                                                               >  3:19098/1‑149 (MQ=255)
   tCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGGTATGATATGTTGGTCGGATAAGGCGCTcgcg                                                                                                                                              >  6:362237/1‑149 (MQ=255)
      tACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGcgccg                                                                                                                                           >  7:168888/1‑149 (MQ=255)
       aCATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGcgccg                                                                                                                                           >  1:355406/1‑148 (MQ=255)
         aTTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCAt                                                                                                                                        <  5:123732/149‑1 (MQ=255)
         aTTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCGAGcgccttat                                                                                                                                        >  7:230216/1‑145 (MQ=255)
          ttAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATAGGTAAATAGCTTGCCTGATCTTATTCTTTCGGTCATTGGCATGTTCAATGAGATCACTCCGTTATGATATTTTGGTCGGATAAGGCGCTCGAGCAGCATc                                                                                                                                       >  7:138067/1‑149 (MQ=255)
            aaCGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCGAGCGCCTTATcc                                                                                                                                      <  8:230216/148‑1 (MQ=255)
             aCGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGa                                                                                                                                    <  6:105530/149‑1 (MQ=255)
               gTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGGCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACa                                                                                                                                  <  8:396982/149‑1 (MQ=255)
                ttttCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACTTATCGGTAAATATCTTGCCTGCTCTTATTATTTCTGTCATTGTCATGTTCCATGCGATCAATCCGTTATTATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACat                                                                                                                                 <  7:281621/149‑1 (MQ=255)
                      cACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACattgattg                                                                                                                           >  7:235029/1‑149 (MQ=255)
                      cACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGCTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACattgattg                                                                                                                           <  5:362237/149‑1 (MQ=255)
                       aCGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTGCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGc                                                                                                                          <  1:290947/149‑1 (MQ=255)
                       aCGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCGGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGc                                                                                                                          <  5:320840/149‑1 (MQ=255)
                           aGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTGCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTaa                                                                                                                      <  5:229882/149‑1 (MQ=255)
                             tCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCtt                                                                                                                        <  3:136979/145‑1 (MQ=255)
                             tCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCtt                                                                                                                        >  4:136979/1‑145 (MQ=255)
                             tCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGAGCAGCATCCGACATTTATTGCTTAAGc                                                                                                                    >  5:271337/1‑149 (MQ=255)
                               ttttttCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCAGGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATACGACATTGATTGCTTAAGCGa                                                                                                                  >  3:145177/1‑149 (MQ=255)
                                 ttttCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCg                                                                                                                   <  4:200659/146‑1 (MQ=255)
                                 ttttCCGGTAAGACTTCGGACATATCGGTAAATAGATTGCCTGCTCTTATTCTTTCTGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGa                                                                                                                  <  8:138067/147‑1 (MQ=255)
                                  tttCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACt                                                                                                                <  7:180314/148‑1 (MQ=255)
                                      cGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACttcatt                                                                                                           <  4:309736/149‑1 (MQ=255)
                                      cGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACttcatt                                                                                                           <  5:8649/149‑1 (MQ=255)
                                      cGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACttcatt                                                                                                           <  3:166412/149‑1 (MQ=255)
                                      cGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACttcat                                                                                                            <  7:90506/148‑1 (MQ=255)
                                        gTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACttcattca                                                                                                         <  6:99302/149‑1 (MQ=255)
                                           aGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCt                                                                                                      <  2:336613/149‑1 (MQ=255)
                                             ccTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTga                                                                                                    <  4:365729/149‑1 (MQ=255)
                                                  gCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGacgacg                                                                                               <  8:59007/149‑1 (MQ=255)
                                                    acaTATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCtt                                                                                                                        <  5:27573/122‑1 (MQ=255)
                                                    acaTATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCtt                                                                                                                        >  6:27573/1‑122 (MQ=255)
                                                    acaTATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGc                                                                                                                    >  3:86602/1‑126 (MQ=255)
                                                    acaTATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGc                                                                                                                    <  4:86602/126‑1 (MQ=255)
                                                    acaTATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGacgacg                                                                                               >  1:220976/1‑147 (MQ=255)
                                                     caTATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACgcag                                                                                            <  5:321197/149‑1 (MQ=255)
                                                     caTATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACgcag                                                                                            <  2:226769/149‑1 (MQ=255)
                                                           ggTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAggg                                                                                       <  8:273868/148‑1 (MQ=255)
                                                            gTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACattgatt                                                                                                                            >  1:128224/1‑110 (MQ=255)
                                                            gTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACattgatt                                                                                                                            >  1:122787/1‑110 (MQ=255)
                                                            gTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACattgatt                                                                                                                            <  2:128224/110‑1 (MQ=255)
                                                            gTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACattgatt                                                                                                                            <  2:122787/110‑1 (MQ=255)
                                                                 tAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCgg                                                                                >  3:150022/1‑149 (MQ=255)
                                                                  aGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCggg                                                                               >  3:376836/1‑149 (MQ=255)
                                                                     ttGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAgg                                                                                        <  2:218973/137‑1 (MQ=255)
                                                                     ttGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAgg                                                                                        >  1:218973/1‑137 (MQ=255)
                                                                         cTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCgggccggggc                                                                        <  2:198504/149‑1 (MQ=255)
                                                                          tGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACttc                                                                                                              <  6:220444/110‑1 (MQ=255)
                                                                          tGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACttc                                                                                                              >  5:220444/1‑110 (MQ=255)
                                                                             tctTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGc                                                                     >  3:79993/1‑148 (MQ=255)
                                                                               ttATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCt                                                                    >  7:43240/1‑147 (MQ=255)
                                                                               ttATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCt                                                                    >  1:161808/1‑147 (MQ=255)
                                                                               ttATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCAGCCGACATTGATTGCTTAAGCGACTTCATTCCCCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCt                                                                    >  3:336207/1‑147 (MQ=255)
                                                                                tATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAg                                                                 >  1:196612/1‑149 (MQ=255)
                                                                                 aTTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCGAAGc                                                                >  2:321988/1‑149 (MQ=255)
                                                                                 aTTCTTTCGGTCATTGGCATGTTCAATCCGATCACTCCGTTTTGATATGTTGGTCGGATAAGGCGCGCCCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTaa                                                                  <  3:327980/147‑1 (MQ=255)
                                                                                  ttCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGc                                                                <  2:394786/148‑1 (MQ=255)
                                                                                  ttCTTTCGGTCATTGGCATGTTCAATGCGATCACTCAGTTATGATAAGTTGGTCGGATAAGGCGCTCGCGCCGCATCAGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCg                                                               >  8:72369/1‑149 (MQ=255)
                                                                                       tCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTg                                                             <  1:288270/146‑1 (MQ=255)
                                                                                          gTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAgg                                                                                        <  7:388001/116‑1 (MQ=255)
                                                                                          gTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAgg                                                                                        >  8:388001/1‑116 (MQ=255)
                                                                                             aTTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGaa                                                    <  2:220976/149‑1 (MQ=255)
                                                                                                 gCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCTGATAAGGCGCTCGCGGCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGACAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTa                                                <  2:274208/149‑1 (MQ=255)
                                                                                                   aTGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAg                                              <  6:364439/149‑1 (MQ=255)
                                                                                                    tGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAgg                                             <  7:326436/149‑1 (MQ=255)
                                                                                                       tCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTg                                           >  1:166678/1‑148 (MQ=255)
                                                                                                         aaTGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAacac                                                        >  4:10681/1‑133 (MQ=255)
                                                                                                         aaTGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAacac                                                        <  3:10681/133‑1 (MQ=255)
                                                                                                         aaTGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGAATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAg                                        >  4:272059/1‑149 (MQ=255)
                                                                                                              gATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAg                                   >  1:153894/1‑149 (MQ=255)
                                                                                                                 cACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAgcgc                                >  8:24735/1‑149 (MQ=255)
                                                                                                                  aCTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAgcgc                                >  5:152873/1‑148 (MQ=255)
                                                                                                                   cTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGcca                              >  3:64199/1‑149 (MQ=255)
                                                                                                                   cTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGcca                              <  4:79993/149‑1 (MQ=255)
                                                                                                                    tCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGccac                             >  7:61547/1‑149 (MQ=255)
                                                                                                                    tCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGccac                             >  2:248674/1‑149 (MQ=255)
                                                                                                                     ccGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGccac                             >  2:182075/1‑148 (MQ=255)
                                                                                                                      cGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGccac                             <  7:24735/147‑1 (MQ=255)
                                                                                                                       gTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGccac                             >  7:20892/1‑146 (MQ=255)
                                                                                                                        ttATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGa                                                            >  5:238002/1‑114 (MQ=255)
                                                                                                                        ttATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGa                                                            <  6:238002/114‑1 (MQ=255)
                                                                                                                           tGATATGTTGGTCGGATAAGGCGCGCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGAcc                       <  7:354483/148‑1 (MQ=255)
                                                                                                                             atatGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCGCCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAg                    >  2:304251/1‑149 (MQ=255)
                                                                                                                              tatGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGACCAGCGCCACCAGACCCAg                    >  8:352528/1‑148 (MQ=255)
                                                                                                                               atcaatgTCGGATGCGGCGCGAGCGCCTTATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCa                  >  6:198354/7‑149 (MQ=38)
                                                                                                                                tgttgtTGGGAAAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTAATTCACCTGCGGACGCTGCAGGGTAAGCGGGCCGGGGCGGCTAAGCGCGAGCACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCa                  <  2:36328/148‑1 (MQ=255)
                                                                                                                                    tgTCGGATGCGGCGCGAGCGCCTTATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGa             <  5:198354/148‑1 (MQ=255)
                                                                                                                                        ggATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGATaa          <  2:166678/148‑1 (MQ=255)
                                                                                                                                         gATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGATAAgc        <  8:20892/149‑1 (MQ=255)
                                                                                                                                         gATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGATAAgc        <  2:153894/149‑1 (MQ=255)
                                                                                                                                         gATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGCTGAAGCCCAGCGCCACCAGACCCAGCACCAGATAAgc        >  4:339179/1‑149 (MQ=255)
                                                                                                                                         gATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCAGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGATAAgc        >  3:164615/1‑149 (MQ=255)
                                                                                                                                          aTAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGATAAgcg       <  8:61547/149‑1 (MQ=255)
                                                                                                                                             aGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAGATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGATAAGCGccc    >  2:83062/1‑149 (MQ=255)
                                                                                                                                             aGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGATAAGCGccc    <  4:208810/149‑1 (MQ=255)
                                                                                                                                              ggCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGATAAGCGCCCt   <  1:219682/149‑1 (MQ=255)
                                                                                                                                               gcgcTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGATAAGCGCCCTg  <  2:196612/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
AACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGATAAGCGCCCTG  >  NC_000913/361754‑362045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: