Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,560,996 G→T 14.7% intergenic (+98/‑372) yffL → / → yffM CPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,560,9960GT14.7% 81.1 / 3.2 49intergenic (+98/‑372)yffL/yffMCPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (24/17);  new base T (2/6);  total (26/23)
Fisher's exact test for biased strand distribution p-value = 1.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

GGAGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGCCTAGAGTTAAACAGAAGAATTATTTCGCC  >  NC_000913/2560850‑2561144
                                                                                                                                                   |                                                                                                                                                     
ggAGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTATTGTTGGGTCAAAAGTTGCAGt                                                                                                                                                      >  5:87320/1‑148 (MQ=255)
    tGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAgg                                                                                                                                                >  6:362613/1‑149 (MQ=255)
      cAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGt                                                                                                                                               <  5:362613/148‑1 (MQ=255)
      cAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGGCAAAAGTTGCCGTTAAGGt                                                                                                                                               <  1:399641/148‑1 (MQ=255)
       aaCGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTgg                                                                                                                                             >  1:356036/1‑149 (MQ=255)
         cGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGt                                                                                                                                           <  8:18518/149‑1 (MQ=255)
         cGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGGCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGt                                                                                                                                           <  7:147270/149‑1 (MQ=255)
              aCCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGTGCTATGTTCGCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGATTGTGATGGGGCAAAAGATGGCGTTAAGGGGTGTGaaat                                                                                                                                      >  4:282561/1‑148 (MQ=255)
              aCCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTACATGGATTCAGGTGTGTCAAAAGTTGACGTTATTTCTGTGTAAAAAGTTGAAGTTAAGgtgtgtaatat                                                                                                                                      >  2:86000/1‑144 (MQ=255)
                cAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCaaaatt                                                                                                                                    >  5:288752/1‑147 (MQ=255)
                   ttattaTTCAAGCCGTACAACATGAAATAACGGAATCAGGCGTGCAATGTTCTAGCCTTTTCTTTTCCTTAATTACATGAATTCAGGTGGGTCAAAAGTTGACGTTAGTGGTGTTACAAAAGTTGCCGTCAAGGTGGGTCTACAGTTg                                                                                                                                  >  7:350182/1‑148 (MQ=255)
                   ttattaTTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGc                                                                                                                                 <  7:11988/149‑1 (MQ=255)
                   ttattaTTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTAAGGTGGGTCAAAAGTTGCCGTTTATGGTTTGTAATATTTTGACTTTAAGGTGGGTGaatttttt                                                                                                                                  >  3:308487/1‑142 (MQ=255)
                   ttattaTTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTAAGGTGGGTCAAAAGTTGCCGTTAGTGATGTGTAAAAATTTGCAGTTAAGGAGTGTCAAGAGTTg                                                                                                                                  >  5:336177/1‑148 (MQ=255)
                     attattCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCaaaa                                                                                                                                      <  1:11061/142‑1 (MQ=255)
                     attattCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAACAGTTGcgg                                                                                                                               >  5:211267/1‑147 (MQ=255)
                     attattCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTACCCTTAATTCCATGAATTCAGGTGGCTCAAAAGTTTCCGTAAGTTGTGGGTCAAAAGATGCCGTTAAGGTGGGTCaaaa                                                                                                                                      >  2:11061/1‑142 (MQ=255)
                      ttattCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAATGTTGCCTTAAAGGTGGGTCAAAATTTg                                                                                                                                  >  1:205995/1‑145 (MQ=255)
                      ttattCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCATAAATTACATGAATTCAGGTCGGTCAAAAGTTGCCGTTAGTGGTGTGTCAAATTATGCCGTAATGGGGGGTCAATAGTTgactg                                                                                                                              >  5:334095/1‑145 (MQ=255)
                            aaGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTAAAAATTTGCAGTTAAGGTGGGTCAAAAGTTGCAGTTTAggg                                                                                                                        >  6:107979/1‑148 (MQ=255)
                            aaGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTGAGTGGTGGGTCAAAAGTTGCCGTTTAGGTGGGTCAAAAGTTGCCgagtaggg                                                                                                                        >  5:327987/1‑142 (MQ=255)
                              gcagTACACCATGAAATAAAGGAATCAGTAGTGCAATTTTCACGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTATTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTgg                                                                                                                      <  4:328964/146‑1 (MQ=37)
                                                  ggAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAATTTGCCGTTAAGGTGGGTCAAAAGTTGCAGTTAAGGTGGGTCAAAAGTTGCCGAGTAAg                                                                                                  >  8:45963/1‑149 (MQ=255)
                                                         ggcggcCACTTTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGTGGCGTCAAAAGTTGCCGTTAGTGGTGGGGCAAAAGTTGCCTTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTcta                                                                                           <  5:108021/149‑1 (MQ=37)
                                                         ggCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTAAAAAGTTGCGGTGAAGGTGGCGCAAAATTTGCCTTTAATGTGGGGGAAAATTTTCCTAGTTAGAATCctc                                                                                           >  3:23727/1‑148 (MQ=255)
                                                         ggCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTACATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGGGGGTAAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTcta                                                                                           <  7:377550/149‑1 (MQ=255)
                                                                                 tttGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGCTGAAAATTTGACTAGTAAGAATTCTACAATTAAGAATTCAAGGCGACgg                                                                    >  1:120079/1‑148 (MQ=37)
                                                                                   tGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAATTTTGCAGTTAAGGTGTGTCAAAAGTTGCCGTTTAGGTGGGTGTAAAGTTGCCGAGTAAGGATTCTACTATTAATAATTCTAGGCGACGGGat                                                                 >  1:344178/1‑148 (MQ=37)
                                                                                    gCCTTAATTCCATGAATTCAGGTGAGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGt                                                                >  7:227476/1‑149 (MQ=255)
                                                                                          aTTCCATGAATTCAGCTGGGTAAAAATGTGCCTTTATTGGTGGGTCAAAAGTTGCAGTTAATGTGGGTAAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTa                                                          <  5:176133/149‑1 (MQ=37)
                                                                                              cATGAATTCAGGGGGCTCAAAATAAGGCCTTAGTGGGGGGTCAAAAGATGCCGTTACGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCt                                                      <  1:195879/149‑1 (MQ=25)
                                                                                               aTGAATTCAGTTTGGTAAAAATAAGCCTTTATGGGTGGGTCATATCTTGCCGTTTAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTc                                                     <  8:353517/149‑1 (MQ=25)
                                                                                                        ggTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAATTTGCGGAGTAAGAATTGTACTATTAAGAATTGTAGGCGACGGGAGTGTTTTAGCGTCCcggtcgg                                             >  1:124521/1‑148 (MQ=37)
                                                                                                              ctaaaaGATGCCGTTATTGTTGCCTAAAAAGCAGCCGTTAATGTGGGGCAAAAGTAGCCTTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATaga                                      <  1:86000/147‑1 (MQ=255)
                                                                                                              ccAAAAGGAGCCTTTAGTGGTGGGTAAAAAGTTGCCTTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATaga                                      <  5:385477/148‑1 (MQ=38)
                                                                                                                 cacgTTGCCGTTAGTGGGGGGTCAAAACTTGCCGTCACGGGGGGTCAAAAGTTGCCTTTAAGGTGCGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATagaga                                    <  7:340837/145‑1 (MQ=255)
                                                                                                                 aaaGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATagagag                                   >  1:316488/1‑149 (MQ=255)
                                                                                                                   aGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGGCGTGAAGGGGGGTCAAAAGGTGCCGAGATAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTc                                 >  2:371509/1‑149 (MQ=38)
                                                                                                                      aggccttACTGGGGGGTAAAAACAGGCCTTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGcc                              <  3:368017/144‑1 (MQ=38)
                                                                                                                        gcGTTACGGGTGGGTCACAACACGCCGTTACGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGCCTa                            <  7:364276/148‑1 (MQ=38)
                                                                                                                        cccttATTGTTGGGTAAATACATGCCTTTAAGGTGGGTAAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGCCTa                            <  2:356036/146‑1 (MQ=38)
                                                                                                                           tttctggtgcGGCAAAAGTGTCCGTTAAGTTGGGTAAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGCCTagag                         <  7:323/145‑1 (MQ=38)
                                                                                                                           ttAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAAGTGGGTCAAAAGTTGACGAGGAAGAATTCTAATATTAAGAATTCTAGCCGACTGGACTTTATAAGCATCAAGGTCCGTCATATAGAGTCGCATagat                         >  6:178068/1‑148 (MQ=21)
                                                                                                                           taaGTGGTGCGTCAAAAGTTCACGATAACGTGGGTCAAAAGATGCCGTAAACGAGGCTCAAAAGTTCCCCACTAAGAATACTACAATAAAGAATTCTCCACGACAGCACTTTATTAGCCTCCAGTACAGTCATAGAGAGTCGCCTagag                         >  6:183829/3‑149 (MQ=255)
                                                                                                                           cttttggtggGCTCATATGATGCCTTTTATGTGGGTCAAAAGTTGCACTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGCCTaga                          <  2:46073/145‑1 (MQ=38)
                                                                                                                           attcaggtggGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGCCTaga                          <  7:40183/144‑1 (MQ=255)
                                                                                                                                  tggGTCAAAAGTTTCAGTTAAGGTGGGTCAATAGTTGCCGTTAAGGTTGGTCAAATGTTGCCGAGTAATTTTTCTACTATTAAGAATTCTAGGCGACGGGAGGTTTTTAGCCTCCCGGTCGGTCAtt                                        <  1:389939/127‑2 (MQ=255)
                                                                                                                                  ggggTCATAAGATGCCTTTAAGGTGGGTCAAAAGGTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGCCTAGAGTTAAACa                  <  3:282561/148‑1 (MQ=255)
                                                                                                                                       tCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCACGGTCGGTCATAGAGAGTCGCCTAGAGTTAAACag                 >  2:103357/1‑146 (MQ=255)
                                                                                                                                                   gTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATaga                                      >  1:245373/1‑113 (MQ=255)
                                                                                                                                                   gTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATaga                                      <  2:245373/113‑1 (MQ=255)
                                                                                                                                                   gTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGCCTAGAGTTAAACAGAAGAATTATTTcgcc  >  2:398078/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GGAGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGCCTAGAGTTAAACAGAAGAATTATTTCGCC  >  NC_000913/2560850‑2561144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: