Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,740,598 C→T 5.8% G185S (GGT→AGT)  aroF ← 3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tyrosine‑repressible

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,740,5980CT 5.8% 215.0 / 2.7 72G185S (GGT→AGT) aroF3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tyrosine‑repressible
Reads supporting (aligned to +/- strand):  ref base C (32/33);  new base T (2/2);  total (35/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01

ACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCAGTCCCATATT  >  NC_000913/2740456‑2740745
                                                                                                                                              |                                                                                                                                                   
acaTGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACTAACCgg                                                                                                                                               >  4:1509/1‑149 (MQ=255)
acaTGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACTAACCgg                                                                                                                                               >  4:6736/1‑149 (MQ=255)
 caTGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCgg                                                                                                                                               >  4:65371/1‑148 (MQ=255)
   tGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCAt                                                                                                                                            <  3:284602/149‑1 (MQ=255)
        gTCCGGATTCCCCTGAGTTTGTAGCAACGCCACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGaac                                                                                                                                       >  5:90833/1‑148 (MQ=255)
             gATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCg                                                                                                                                  >  7:71378/1‑149 (MQ=255)
                  cccTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGccc                                                                                                                                   <  5:200331/143‑1 (MQ=255)
                  cccTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGccc                                                                                                                                   >  6:200331/1‑143 (MQ=255)
                  cccTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGc                                                                                                                             >  5:179860/1‑149 (MQ=255)
                  cccTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGCTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGc                                                                                                                             >  2:94014/1‑149 (MQ=255)
                   ccTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGc                                                                                                                             >  2:165696/1‑148 (MQ=255)
                   ccTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGc                                                                                                                             >  5:315324/1‑148 (MQ=255)
                      gAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCAtt                                                                                                                         >  3:307392/1‑149 (MQ=255)
                         tttGTAGCAACGCAACCTGCCCTGCCTTGTTAATGGCAACAAAACGGTGCTGATGGGCGGCGGCGAGCATAGAGTTAATTGCTGTTGCCATACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGATGCCATTTCa                                                                                                                      >  1:50241/1‑149 (MQ=255)
                         tttGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCAGGAGGCCATTTCa                                                                                                                      >  1:56066/1‑149 (MQ=255)
                          ttGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCAGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATATCAc                                                                                                                     >  5:229452/1‑149 (MQ=255)
                            gTAGCAACGCAACCTGCCCTGCCTGGTTAATGACAACAAAACGGTGAGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACg                                                                                                                    <  6:90833/148‑1 (MQ=255)
                               gcaacgcaacCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGa                                                                                                                <  1:371338/149‑1 (MQ=255)
                               gcaacgcaacCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGGCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGa                                                                                                                <  4:214265/149‑1 (MQ=255)
                               gcaacgcaacCTGCCCTGCCTGGTTAATGCCAACAAAACGGGGCGGCTGGGCGGCGGCGCGCAGAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTATAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGa                                                                                                                <  2:259293/149‑1 (MQ=255)
                                  acgcaacCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGa                                                                                                                <  7:148254/146‑1 (MQ=255)
                                  acgcaacCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGa                                                                                                                >  8:148254/1‑146 (MQ=255)
                                  acgcaacCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGtt                                                                                                             >  2:240816/1‑149 (MQ=255)
                                   cgcaacCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGttt                                                                                                            <  1:165696/149‑1 (MQ=255)
                                   cgcaacCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGttt                                                                                                            <  6:179860/149‑1 (MQ=255)
                                         ctgccctgccTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGAtt                                                                                                      <  4:125158/149‑1 (MQ=255)
                                             cctgcctgGTTAATGCCAACAACACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACGAACCGGCATGGAAAGACCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGt                                                                                                  <  3:35418/149‑1 (MQ=255)
                                              ctgcctgGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGtt                                                                                                 <  8:71378/149‑1 (MQ=255)
                                               tgcctgGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTCCCGACGCTGCCGTTTTTAAAGCCAACCGGCATCGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGAtacagatg                                                                                                >  2:283836/1‑142 (MQ=255)
                                                 gctgGTTAATGCCAACAAAACGGGGAGGCTGGGCGGCGGCGCGCATAGCGTTTATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTa                                                                                              <  1:175023/148‑1 (MQ=255)
                                                 cctgGTTAATGCCAAGAAAACGGTGGGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAATCCAACCGGCATGGATAGCCCGGAGTCCATTTCACGGTGAGTTTGCGATTCCGTTGt                                                                                               <  1:94014/148‑1 (MQ=255)
                                                  ctgGTTAATGACAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCTTTAATTGCTGTTGCCAGACTGCCGTCGGTTCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTa                                                                                              <  2:44191/148‑1 (MQ=255)
                                                     gTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAg                                                                                          >  3:313272/1‑149 (MQ=255)
                                                     gTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGGCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGa                                                                                           <  5:329759/148‑1 (MQ=255)
                                                           gCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCaa                                                                                    >  4:326590/1‑149 (MQ=255)
                                                             caacaaAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAAtt                                                                                  <  3:60165/149‑1 (MQ=255)
                                                             caacaaAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAAtt                                                                                  >  4:43747/1‑149 (MQ=255)
                                                             caacaaAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTGTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAAtt                                                                                  <  5:364019/149‑1 (MQ=255)
                                                              aacaacACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTg                                                                                 >  3:160350/1‑149 (MQ=255)
                                                                caaAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCt                                                                               <  8:35494/149‑1 (MQ=255)
                                                                 aaaaCGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTGTTTACAACCAACCGGCATGGACAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTg                                                                              >  7:346541/1‑149 (MQ=255)
                                                                  aaaCGGTGGGGCTGGGCGGCGGCGCGCATAGCGTTATTTGGTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACGAACCGGCTTGGAAAGCCCGGAGGCCATTTCTCGGTTAGTTTGCGATTCCGTTGTACGATCACCAATTGCTGa                                                                             <  4:307392/149‑1 (MQ=255)
                                                                  aaaCGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGa                                                                             <  3:65371/149‑1 (MQ=255)
                                                                    aCGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAAGTGCTGAc                                                                            >  4:244612/1‑148 (MQ=255)
                                                                       gTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGc                                                                        >  3:170555/1‑149 (MQ=255)
                                                                           ggCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACTAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTaaa                                                                    <  3:6736/149‑1 (MQ=255)
                                                                           ggCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACTAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTaaa                                                                    <  3:1509/149‑1 (MQ=255)
                                                                            gCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCAt                                                                                                                                            >  4:73175/1‑76 (MQ=255)
                                                                            gCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCAt                                                                                                                                            <  3:73175/76‑1 (MQ=255)
                                                                              tGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTaaa                                                                    >  4:122607/1‑146 (MQ=255)
                                                                                ggcggcgggGCGGATATGGTTAATTGCTGTTGGCAGAGTGCCGTCGTTTCCGTTTTTAAAACCAACCGGCATGGAAAGCTCGGAGGCCATTTCACGGTGAGTTTGGGATTCCGTTGTACGAGCACCAATTTCTGACCAGCTAATCAGa                                                                <  6:229452/148‑1 (MQ=255)
                                                                                ggcggcggcGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGa                                                                <  5:165764/148‑1 (MQ=255)
                                                                                      ggcgCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGcc                                                           >  7:22359/1‑147 (MQ=255)
                                                                                          cgcATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTa                                                     <  8:405557/149‑1 (MQ=255)
                                                                                                 cGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCggg                                              <  3:326590/149‑1 (MQ=255)
                                                                                                       ttGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTc                                        >  3:101727/1‑149 (MQ=255)
                                                                                                            gTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATc                                   >  1:93255/1‑149 (MQ=255)
                                                                                                             tttccAGAATGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGTAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGAt                                    <  2:50241/144‑1 (MQ=255)
                                                                                                             ttGCCAGACTGCCGTCGTTGCCGTTTTTAAAACCAACCGGCATTGAAAGCCAGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTTCGGGCTATTCGGAt                                    <  2:56066/147‑1 (MQ=255)
                                                                                                             ttGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGAt                                    <  6:315324/147‑1 (MQ=255)
                                                                                                             ttGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGAt                                    <  3:239168/147‑1 (MQ=255)
                                                                                                               gCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTaa                                >  2:129903/1‑149 (MQ=255)
                                                                                                                 cAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACg                              >  6:375077/1‑149 (MQ=255)
                                                                                                                    aCTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCtt                           <  4:170555/149‑1 (MQ=255)
                                                                                                                        ccGTCGGCGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTcc                         <  7:19523/147‑1 (MQ=255)
                                                                                                                         cGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTc                      >  4:369027/1‑149 (MQ=255)
                                                                                                                           tCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGc                    <  8:22359/149‑1 (MQ=255)
                                                                                                                              gTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAg                 >  5:171630/1‑149 (MQ=255)
                                                                                                                                 ccGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTg               <  1:240816/148‑1 (MQ=255)
                                                                                                                                  cGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTgg              >  3:243881/1‑148 (MQ=255)
                                                                                                                                   gTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCa            <  4:313272/149‑1 (MQ=255)
                                                                                                                                   gTTTTTAAAACAAACCGGCATGGAAAGCCCGGAGGCCAATTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAAATGCTGACCAGCTAAACAGATCGACCAGGTATTGCTGGCTATTCGGATCTAACGCTTCCGTCTCAAGtcg              >  6:197174/1‑145 (MQ=255)
                                                                                                                                             ccaaccGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAACCAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCAGTCCCATAtt  <  4:101727/149‑1 (MQ=255)
                                                                                                                                              caaccGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCAGTCCCATAtt  >  5:29735/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
ACATGGCCGTCCGGATTCCCCTGAGTTTGTAGCAACGCAACCTGCCCTGCCTGGTTAATGCCAACAAAACGGTGCGGCTGGGCGGCGGCGCGCATAGCGTTAATTGCTGTTGCCAGACTGCCGTCGGTGCCGTTTTTAAAACCAACCGGCATGGAAAGCCCGGAGGCCATTTCACGGTGAGTTTGCGATTCCGTTGTACGAGCACCAATTGCTGACCAGCTAAACAGATCGCCCAGGTATTGCGGGCTATTCGGATCTAACGCTTCCGTCGCCAGTGGCAGTCCCATATT  >  NC_000913/2740456‑2740745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: