Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,424,646 C→A 6.9% noncoding (2138/2904 nt) rrlD ← 23S ribosomal RNA of rrnD operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,424,6460CA 6.9% 498.3 / 11.6 196noncoding (2138/2904 nt)rrlD23S ribosomal RNA of rrnD operon
Reads supporting (aligned to +/- strand):  ref base C (100/80);  new base A (2/14);  total (102/94)
Fisher's exact test for biased strand distribution p-value = 1.11e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 201 total aligned reads displayed.

CTCCGTTACTCTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGGAGAC  >  NC_000913/3424505‑3424792
                                                                                                                                             |                                                                                                                                                  
ctccGTTACTCTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCt                                                                                                                                              >  2:37820/1‑148 (MQ=11)
ctccGTTACTCTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCt                                                                                                                                              <  1:252782/148‑1 (MQ=11)
ctccGTTACTCTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCt                                                                                                                                              <  2:321557/148‑1 (MQ=11)
ctccGTTACTCTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGGCCACACTTCt                                                                                                                                              >  7:129108/1‑148 (MQ=11)
   cGTTACTCTTTAGGAGGAGACCGCCCCAGTCAAAGTACCCACCAGACAGTGTCCGCAAGCCGGATTACGTGTCAACGTTAGAACATCTAACATTATAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACAGTTCTaa                                                                                                                                            <  6:389129/147‑1 (MQ=11)
   cGTTACTCTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTaa                                                                                                                                            <  2:2750/147‑1 (MQ=32)
   cGTTACTCTTTAGGAGGAGACCGCCCCACTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGc                                                                                                                                          <  1:355970/149‑1 (MQ=17)
    gTTACTCTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGcc                                                                                                                                         >  4:50217/1‑149 (MQ=32)
             tAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCAcc                                                                                                                                  <  6:35873/147‑1 (MQ=32)
               ggaggaGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATc                                                                                                                              >  1:385528/1‑149 (MQ=32)
                gaggagACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATcc                                                                                                                             <  2:182720/149‑1 (MQ=32)
                  ggagACCGCCCCAGTCAAACTACCCAACAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGACCATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTa                                                                                                                           <  7:296257/149‑1 (MQ=17)
                   gagaCCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTac                                                                                                                          <  1:16496/149‑1 (MQ=32)
                      aCCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTacac                                                                                                                        >  8:235358/1‑148 (MQ=32)
                      aCCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTATAAg                                                                                                                                           >  2:144865/1‑129 (MQ=14)
                      aCCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTATAAg                                                                                                                                           <  1:144865/129‑1 (MQ=14)
                          ccccAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATc                                                                                                                     >  2:152771/1‑147 (MQ=32)
                           cccAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTTGCGTCCAACCTTCTAAGCCTCCCACCTATCCTACACATCAAg                                                                                                                  >  4:14639/1‑149 (MQ=12)
                           cccAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAg                                                                                                                  >  1:106950/1‑149 (MQ=32)
                           cccAGTCAAACTACCCACCAGACACTGTCCGCAACACGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGGACACACTTCTAAGCCTCCCACCTATCCTACAACTCAAg                                                                                                                  >  3:15742/1‑149 (MQ=11)
                            ccAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAgg                                                                                                                 <  7:235358/149‑1 (MQ=32)
                              aGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCt                                                                                                               <  3:62183/149‑1 (MQ=32)
                                  aaaCTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCaa                                                                                                            <  7:222882/148‑1 (MQ=32)
                                  aaaCTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAAt                                                                                                           <  5:16669/149‑1 (MQ=32)
                                  aaaCTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAAt                                                                                                           <  1:34154/149‑1 (MQ=32)
                                   aaCTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCaagg                                                                                                          >  5:209931/1‑147 (MQ=17)
                                     cTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGt                                                                                                         >  8:163287/1‑148 (MQ=32)
                                        cccACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCa                                                                                                      <  1:267312/148‑1 (MQ=32)
                                        cccACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCa                                                                                                      <  7:274814/148‑1 (MQ=32)
                                         ccaccaGACACTGTCCGCCACCCGGCTTACGGGTCAACGGTAGACCCTCAAACATTAAAGGGGGGTATTTCAAGGGCGGCTCCATGCAGACTTTTTTTTTAATTTTTATGCGTCCAACGTTTTCTACACATCAAGGCTCAATGTTCAgt                                                                                                    <  2:345332/149‑1 (MQ=2)
                                         ccaccaGACACTGCCCGCAACCCGGATTACGGGTCAACGCTACACCCTCCCACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGATTTTTTTTTTAATGTTTATGCGTCCAACGTTTTCTACACATCAAGGCTCAATGTTCAgt                                                                                                    <  6:176358/149‑1 (MQ=2)
                                           accaGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAgtgt                                                                                                  >  6:72697/1‑149 (MQ=32)
                                             caGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTc                                                                                                 >  3:40437/1‑148 (MQ=32)
                                                  acTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTAACCTACACATCAAGTCACAATGATCAGTGACAAGCta                                                                                           >  2:227819/1‑149 (MQ=11)
                                                    tGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCa                                                                                                      <  4:194489/136‑1 (MQ=32)
                                                    tGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCta                                                                                           >  1:112702/1‑147 (MQ=32)
                                                    tGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCta                                                                                           >  3:225159/1‑147 (MQ=32)
                                                      tCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAg                                                                                        <  7:56744/148‑1 (MQ=32)
                                                      tCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCAAACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGt                                                                                       <  2:106950/149‑1 (MQ=17)
                                                      tCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACAGTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCCGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTCCACATCAAGGCTGAATGTTCAGTGTCAAGCTATAg                                                                                        >  3:25571/1‑148 (MQ=255)
                                                        cGCAACCCGGATTACGGGTCAATGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTa                                                                                      >  4:289116/1‑148 (MQ=17)
                                                         gCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTaaa                                                                                    <  1:381916/149‑1 (MQ=32)
                                                           aaCCCGGAGTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTCGTATTTCTAGGTAGGCTCCATGCAGATTTTTTTTTAAATTTTTATGCGTCGAACGATTTCTTCACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAgg                                                                                  <  2:401980/149‑1 (MQ=2)
                                                              ccGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAg                                                                                                     >  4:266438/1‑127 (MQ=32)
                                                              ccGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGtt                                                                                >  1:328047/1‑148 (MQ=32)
                                                               cGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCa                                                                              >  7:146933/1‑149 (MQ=32)
                                                                 gATTACGGGTCAACGTTAGAACATCAAACATTACAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACg                                                                            <  5:316101/149‑1 (MQ=17)
                                                                     aCGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACgggg                                                                         <  1:37820/148‑1 (MQ=32)
                                                                             aCGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGt                                                                >  7:205713/1‑149 (MQ=32)
                                                                             aCGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGt                                                                >  4:99195/1‑149 (MQ=32)
                                                                               gTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGc                                                                                                                                          <  4:221325/73‑1 (MQ=34)
                                                                               gTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCt                                                              >  6:13586/1‑149 (MQ=32)
                                                                                ttAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGACtt                                                             >  8:301021/1‑149 (MQ=17)
                                                                                ttAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACATATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCtt                                                             >  8:190283/1‑149 (MQ=17)
                                                                                  aGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTg                                                            <  1:88345/148‑1 (MQ=32)
                                                                                    aaCATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAg                                                                                   >  2:20214/1‑123 (MQ=32)
                                                                                    aaCATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCcg                                                         >  6:49632/1‑149 (MQ=32)
                                                                                    aaCATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCcg                                                         >  8:221273/1‑149 (MQ=32)
                                                                                     aCATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCcgc                                                        <  7:105314/149‑1 (MQ=32)
                                                                                       aTCAAACATTAAAGTGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTCTCCTACACATCAAGGCTCAATGTTCAGTGTCTAGCTATAGTAAATGCTCTCGGGGTCTTTCCGTCTTGCCGCgg                                                      <  5:275462/149‑1 (MQ=11)
                                                                                       aTCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCgg                                                      <  3:213495/149‑1 (MQ=32)
                                                                                        tCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCggg                                                     <  4:254932/149‑1 (MQ=32)
                                                                                         cAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTc                                                                                                       <  1:54504/98‑1 (MQ=33)
                                                                                         cAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTc                                                                                                       >  2:54504/1‑98 (MQ=33)
                                                                                          aaaCATTAAAGGCTGGTATTTCAAGGTCGGCTCCATGCAGACTGGTGTTTAAACTTTTATGCCTCCCACCAATGCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTc                                                               <  8:26823/137‑1 (MQ=11)
                                                                                              aTTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTTGCGTACACAATTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTGCGTCTTGCCGCGGGTACACt                                               <  2:328047/149‑1 (MQ=255)
                                                                                              aTTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACGTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACt                                               <  2:352348/149‑1 (MQ=17)
                                                                                              aTTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACt                                               <  7:163287/149‑1 (MQ=32)
                                                                                                   aGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATc                                          <  5:49632/149‑1 (MQ=32)
                                                                                                   aGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATc                                          <  5:72697/149‑1 (MQ=32)
                                                                                                     ggtggtATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCt                                         >  6:202474/1‑148 (MQ=32)
                                                                                                      gtggtATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCa                                                                                                      >  1:345758/1‑86 (MQ=33)
                                                                                                       tggtATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGt                                                                        <  1:227024/115‑1 (MQ=32)
                                                                                                        ggtATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTca                                      >  8:78971/1‑148 (MQ=32)
                                                                                                           aTTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGc                                  >  4:100027/1‑149 (MQ=32)
                                                                                                               cAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACATATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCAATAGTAAAGGTTCACGGGGTCTTAACGTCTTGCCGAGGGTACACAGCATCTTCACAGCGAGt                              >  5:164687/1‑149 (MQ=11)
                                                                                                                aaGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGGCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCGTCACAGCGAGtt                             >  3:308166/1‑149 (MQ=17)
                                                                                                                 aGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACCGCGAGtt                             >  4:279465/1‑148 (MQ=17)
                                                                                                                  ggTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTc                            >  5:248081/1‑148 (MQ=32)
                                                                                                                        cTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAAtt                        <  1:186903/146‑1 (MQ=32)
                                                                                                                        cTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCa                     <  5:13586/149‑1 (MQ=32)
                                                                                                                        atCCCTGCAGACTGGCGTCCACACTTCTAAGCCTCCCGCCGATCCTCCCCATAAAGTCTGAATGTACAGTGGCCAGCTAGAGGAAAGGTTCCCGGGGTCTGTCCGTCTGGCCGCGGGTACACTGCATCTTACAAGCGAGTTCAATTTCa                     >  7:281701/2‑149 (MQ=2)
                                                                                                                         tacATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTaca                                                 <  5:200712/118‑1 (MQ=17)
                                                                                                                            aTGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACt                   >  4:362212/1‑147 (MQ=32)
                                                                                                                            aTGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACt                   >  7:364690/1‑147 (MQ=32)
                                                                                                                              gCAGACTGGCGTCCACACTTCTAAGCCTCCCACCATTCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCCCGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGGTCACTTTCCCTGAGt               >  4:34980/1‑149 (MQ=11)
                                                                                                                                 gACTGGCGTCCACACTTCTAAGCCTCCCACGTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGtctc            <  8:105484/149‑1 (MQ=17)
                                                                                                                                 gACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGtctc            <  3:289116/149‑1 (MQ=32)
                                                                                                                                  ccTGCCGACCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGAGTCAACAGATACTAAAGGTTCACGGCGTCATTCCGTCTTGCATCGGGAACACTGCATCTTCACCGCGACTTCAATTTCAATGAGtctcc           >  2:246591/2‑148 (MQ=11)
                                                                                                                                    tGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCaa                          >  8:349098/1‑132 (MQ=32)
                                                                                                                                      gCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCCCggg         <  8:146933/147‑1 (MQ=17)
                                                                                                                                        gTCCACACTTCTAAGCCTCCCACCTATCCTACTCATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGga     <  4:225159/149‑1 (MQ=17)
                                                                                                                                        gTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTGCACGGGGGCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGga     <  5:272725/149‑1 (MQ=17)
                                                                                                                                         tCCACACTTCTAAGCCTCCCACCTATGCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTg       >  8:104213/1‑146 (MQ=17)
                                                                                                                                         tCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGgag    >  6:279104/1‑149 (MQ=32)
                                                                                                                                         tCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGgag    >  6:261161/1‑149 (MQ=32)
                                                                                                                                           cacaCTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGgaga   >  8:297590/1‑148 (MQ=32)
                                                                                                                                           cacaCTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGGAGAc  <  8:364690/149‑1 (MQ=32)
                                                                                                                                           cacaCTTCTAAGCCTCCCAACTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCCCGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGgaga   >  3:117879/1‑148 (MQ=17)
                                                                                                                                            acacTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCAGGTGgaga   >  5:287049/1‑147 (MQ=17)
                                                                                                                                             |                                                                                                                                                  
CTCCGTTACTCTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGGAGAC  >  NC_000913/3424505‑3424792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: