Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,424,661 C→A 5.7% noncoding (2123/2904 nt) rrlD ← 23S ribosomal RNA of rrnD operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,424,6610CA 5.7% 491.2 / 9.9 196noncoding (2123/2904 nt)rrlD23S ribosomal RNA of rrnD operon
Reads supporting (aligned to +/- strand):  ref base C (102/80);  new base A (3/10);  total (105/91)
Fisher's exact test for biased strand distribution p-value = 4.00e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 196 total aligned reads displayed.

TCTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGGAGACAGCCTGGC  >  NC_000913/3424514‑3424800
                                                                                                                                                   |                                                                                                                                           
tctTTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCa                                                                                                                                            >  4:359484/1‑149 (MQ=32)
       gaggagACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATcc                                                                                                                                     <  2:182720/149‑1 (MQ=32)
          gagaCCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTac                                                                                                                                  <  1:16496/149‑1 (MQ=32)
           agaCCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTaca                                                                                                                                 <  7:28067/149‑1 (MQ=32)
           agaCCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTaca                                                                                                                                 <  5:262302/149‑1 (MQ=32)
             aCCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTacac                                                                                                                                >  8:235358/1‑148 (MQ=32)
              ccGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACAt                                                                                                                              <  5:243231/149‑1 (MQ=32)
               cGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATc                                                                                                                             <  7:398270/149‑1 (MQ=32)
                 ccccAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATc                                                                                                                             >  2:152771/1‑147 (MQ=32)
                  cccAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAg                                                                                                                          >  1:106950/1‑149 (MQ=32)
                  cccAGTCAAACTACCCACCAGACACTGTCCGCAACACGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGGACACACTTCTAAGCCTCCCACCTATCCTACAACTCAAg                                                                                                                          >  3:15742/1‑149 (MQ=11)
                     aGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCt                                                                                                                       >  8:85481/1‑149 (MQ=32)
                       tCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTc                                                                                                                      >  8:56744/1‑148 (MQ=32)
                         aaaCTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAAt                                                                                                                   <  5:16669/149‑1 (MQ=32)
                         aaaCTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAAt                                                                                                                   <  1:34154/149‑1 (MQ=32)
                          aaCTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCaagg                                                                                                                  >  5:209931/1‑147 (MQ=17)
                           aCTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGt                                                                                                                 >  2:288041/1‑149 (MQ=32)
                            cTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGt                                                                                                                 >  8:163287/1‑148 (MQ=32)
                               cccACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCa                                                                                                              <  1:267312/148‑1 (MQ=32)
                                ccaccaGCCACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTTTTTTTTAAATTTTTATGCGTCCCACGTTTTCTACACATCAAGGCTCAATGTTCAgt                                                                                                            <  4:1388/149‑1 (MQ=11)
                                ccaccaGACACTGTCCGCCACCCGGCTTACGGGTCAACGGTAGACCCTCAAACATTAAAGGGGGGTATTTCAAGGGCGGCTCCATGCAGACTTTTTTTTTAATTTTTATGCGTCCAACGTTTTCTACACATCAAGGCTCAATGTTCAgt                                                                                                            <  2:345332/149‑1 (MQ=2)
                                ccaccaGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAgt                                                                                                            <  5:33160/149‑1 (MQ=32)
                                ccaccaGACACTGCCCGCAACCCGGATTACGGGTCAACGCTACACCCTCCCACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGATTTTTTTTTTAATGTTTATGCGTCCAACGTTTTCTACACATCAAGGCTCAATGTTCAgt                                                                                                            <  6:176358/149‑1 (MQ=2)
                                 caccaGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGATTTTTTTTTTAATTTTTATGCGTCCAACGTTTTCTACACATCAAGGCTCAATGTTCAgtg                                                                                                           <  8:358003/149‑1 (MQ=11)
                                  accaGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCt                                                                                                                       <  1:42325/136‑1 (MQ=32)
                                  accaGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAgtgt                                                                                                          >  6:72697/1‑149 (MQ=32)
                                    caGACACTGTCCGCAACCCGGATTACGGGTCAACGGTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGATTTTTTTTTTAATTTTTATGCGTCCAACGTTTTCTACACATCAAGGCTCAATGTTCAGTGTCa                                                                                                        <  4:57578/149‑1 (MQ=11)
                                      gACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAg                                                                                                      >  2:107498/1‑149 (MQ=32)
                                         acTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTAACCTACACATCAAGTCACAATGATCAGTGACAAGCta                                                                                                   >  2:227819/1‑149 (MQ=11)
                                           tGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCta                                                                                                   >  3:254932/1‑147 (MQ=32)
                                             tCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAg                                                                                                <  7:56744/148‑1 (MQ=32)
                                               cGCAACCCGGATTACGGGTCAATGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTa                                                                                              >  4:289116/1‑148 (MQ=17)
                                                  aaCCCGGAGTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTCGTATTTCTAGGTAGGCTCCATGCAGATTTTTTTTTAAATTTTTATGCGTCGAACGATTTCTTCACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAgg                                                                                          <  2:401980/149‑1 (MQ=2)
                                                     ccGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAg                                                                                                             >  4:266438/1‑127 (MQ=32)
                                                     ccGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGtt                                                                                        >  1:328047/1‑148 (MQ=32)
                                                      cGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCa                                                                                      >  7:146933/1‑149 (MQ=32)
                                                                   aaCGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTGCACGGGGTCTTTcc                                                                          <  1:82181/148‑1 (MQ=17)
                                                                      gTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCt                                                                      >  6:13586/1‑149 (MQ=32)
                                                                       ttAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGACtt                                                                     >  8:301021/1‑149 (MQ=17)
                                                                       ttAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACATATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCtt                                                                     >  8:190283/1‑149 (MQ=17)
                                                                         aGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTg                                                                    <  1:107498/148‑1 (MQ=32)
                                                                           aaCATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAg                                                                                           <  1:20214/123‑1 (MQ=32)
                                                                           aaCATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAg                                                                                           >  2:20214/1‑123 (MQ=32)
                                                                           aaCATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCcg                                                                 >  6:49632/1‑149 (MQ=32)
                                                                           aaCATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCcg                                                                 >  8:221273/1‑149 (MQ=32)
                                                                            aCATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCcgc                                                                <  7:105314/149‑1 (MQ=32)
                                                                              aTCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCgg                                                              <  3:213495/149‑1 (MQ=32)
                                                                               tCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCATAATGGCGTCCACACTTCTAAGCCTCAAACCTATACTACACATCAAGGCTCAATTTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGACTCggg                                                             >  5:287648/1‑149 (MQ=11)
                                                                               tCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCggg                                                             <  4:254932/149‑1 (MQ=32)
                                                                                cAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTc                                                                                                               >  2:54504/1‑98 (MQ=33)
                                                                                 aaaCATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCATTCCGTc                                                                       >  7:26823/1‑137 (MQ=17)
                                                                                 aaaCATTAAAGGCTGGTATTTCAAGGTCGGCTCCATGCAGACTGGTGTTTAAACTTTTATGCCTCCCACCAATGCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTc                                                                       <  8:26823/137‑1 (MQ=11)
                                                                                  aaCATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTa                                                           >  1:134128/1‑148 (MQ=32)
                                                                                  aaCATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTa                                                           >  2:359382/1‑148 (MQ=32)
                                                                                     aTTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTTGCGTACACAATTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTGCGTCTTGCCGCGGGTACACt                                                       <  2:328047/149‑1 (MQ=255)
                                                                                     aTTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACt                                                       <  7:163287/149‑1 (MQ=32)
                                                                                          atggttgtATTTCAAGGTAGGCTCCATGCAGACTGTCTTACTAACTTATAAGCCTACAACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATc                                                  <  4:15742/147‑1 (MQ=11)
                                                                                          aGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATc                                                  <  5:72697/149‑1 (MQ=32)
                                                                                          aGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATc                                                  <  5:49632/149‑1 (MQ=32)
                                                                                            ggtggtATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCt                                                 >  6:202474/1‑148 (MQ=32)
                                                                                             gtggtATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCa                                                                                                              >  1:345758/1‑86 (MQ=33)
                                                                                             gtggtATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCa                                                                                                              <  2:345758/86‑1 (MQ=33)
                                                                                             gtggtATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCtt                                                >  6:355373/1‑148 (MQ=32)
                                                                                                  aTTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCAc                                                                                     >  3:262600/1‑106 (MQ=32)
                                                                                                     tCAAGGTCGGCTCCATGCAGACTGGCGTCCCCACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCCCGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAg                                       >  1:245628/1‑149 (MQ=17)
                                                                                                     tCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGa                                        <  7:221273/148‑1 (MQ=32)
                                                                                                      cAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACATATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCAATAGTAAAGGTTCACGGGGTCTTAACGTCTTGCCGAGGGTACACAGCATCTTCACAGCGAGt                                      >  5:164687/1‑149 (MQ=11)
                                                                                                       aaGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACCCATCCAGGCTCAATGTTCAGTGTCAAGCTAGAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGtt                                     >  6:381723/1‑149 (MQ=12)
                                                                                                        aGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACCGCGAGtt                                     >  4:279465/1‑148 (MQ=17)
                                                                                                           tCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAAt                                 <  3:46941/149‑1 (MQ=32)
                                                                                                             ggCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAAttt                               <  2:385528/149‑1 (MQ=32)
                                                                                                              gCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAAttt                               >  5:273643/1‑148 (MQ=32)
                                                                                                              gCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTc                              <  5:11201/149‑1 (MQ=32)
                                                                                                               cTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAAtt                                <  1:186903/146‑1 (MQ=32)
                                                                                                               cTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCa                             <  5:13586/149‑1 (MQ=32)
                                                                                                               cTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCa                             >  7:128490/1‑149 (MQ=32)
                                                                                                                tacATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTaca                                                         <  5:200712/118‑1 (MQ=17)
                                                                                                                  cATTCAGACTGGAGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACt                           <  1:91460/148‑1 (MQ=17)
                                                                                                                  cATGCAGACTGGCGTCAACACTTCTAAGCCTCCCCCCTATCCTAGACATCAAGGCTCAATGTTGAGTGTCAAGCTATAGTAAAGGTTCACGGTGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCTCAGCGAGTTCAATTTCACt                           <  1:383425/148‑1 (MQ=11)
                                                                                                                   aTGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACt                           >  4:362212/1‑147 (MQ=32)
                                                                                                                   aTGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGa                         <  3:99195/149‑1 (MQ=32)
                                                                                                                    tGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATAAAGGCTCAATGTTCAGTCTCAATCTATAGTAAAGGTTCACGGGGTCTTTCCGACTTGCCGCGGTTAAACTGCATCTTCACAGCTAGTTCAATTTCCCTGAg                        >  5:27729/1‑149 (MQ=11)
                                                                                                                     gCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGt                       >  1:378760/1‑149 (MQ=32)
                                                                                                                      cAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGt                       <  7:289852/148‑1 (MQ=32)
                                                                                                                        gACTGGCGTCCACACTTCTAAGCCTCCCACGTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGtctc                    <  8:105484/149‑1 (MQ=17)
                                                                                                                         ccTGCCGACCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGAGTCAACAGATACTAAAGGTTCACGGCGTCATTCCGTCTTGCATCGGGAACACTGCATCTTCACCGCGACTTCAATTTCAATGAGtctcc                   >  2:246591/2‑148 (MQ=11)
                                                                                                                          cTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCgg                  <  7:233074/149‑1 (MQ=32)
                                                                                                                           tGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCaa                                  <  7:349098/132‑1 (MQ=32)
                                                                                                                             gCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTg               >  1:363721/1‑149 (MQ=32)
                                                                                                                             gCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCCCggg                 <  8:146933/147‑1 (MQ=17)
                                                                                                                             gCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTCAGTCTCGGGTg               >  3:29697/1‑149 (MQ=17)
                                                                                                                             gCGTCCACACTTCTAAGCCTCCCACCTATACTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTg               <  4:211191/149‑1 (MQ=17)
                                                                                                                               gTCCACACTTCTAAGCCTCCCACCTATCCTACTCATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGga             <  4:225159/149‑1 (MQ=17)
                                                                                                                                tCCACACTTCTAAGCCTCCCACCTATGCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTg               >  8:104213/1‑146 (MQ=17)
                                                                                                                                tCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTg               >  3:40247/1‑146 (MQ=32)
                                                                                                                                tCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGgag            >  6:261161/1‑149 (MQ=32)
                                                                                                                                  cacaCTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGgaga           >  8:297590/1‑148 (MQ=32)
                                                                                                                                  cacaCTTCTAAGCCTCCCAACTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCCCGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGgaga           >  3:117879/1‑148 (MQ=17)
                                                                                                                                         cTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTg               <  3:324973/137‑1 (MQ=31)
                                                                                                                                         cTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTg               >  4:324973/1‑137 (MQ=31)
                                                                                                                                                   |                                                                                                                                           
TCTTTAGGAGGAGACCGCCCCAGTCAAACTACCCACCAGACACTGTCCGCAACCCGGATTACGGGTCAACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGGAGACAGCCTGGC  >  NC_000913/3424514‑3424800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: