Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,934,587 T→C L6L (TTA→TTG edd ← 6‑phosphogluconate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,934,5870TC99.4% 634.5 / NA 174L6L (TTA→TTGedd6‑phosphogluconate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (85/88);  minor base G (0/1);  total (85/89)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 178 total aligned reads displayed.

GGCTGGCAGGCAGCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAA  >  NC_000913/1934444‑1934732
                                                                                                                                               |                                                                                                                                                 
ggctggcAGGCAGCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGcaacaa                                                                                                                                              >  5:552923/1‑149 (MQ=255)
ggctggcAGGCAGCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGAGAACGATGAACGGTCGAAGTTGTCGCGTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTACAATGATTCGATTAGATACGCGcaacaa                                                                                                                                              >  5:391651/1‑149 (MQ=255)
 gctggcAGGCAGCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAAt                                                                                                                                             >  4:63871/1‑149 (MQ=255)
 gctggcAGGCAGCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAAt                                                                                                                                             >  4:217504/1‑149 (MQ=255)
   tggcAGGCAGCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATtg                                                                                                                                           >  8:154460/1‑149 (MQ=255)
    ggcaggcaGCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATtgt                                                                                                                                          <  6:253549/149‑1 (MQ=255)
     gcaggcagCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATtgtg                                                                                                                                         <  5:655471/149‑1 (MQ=255)
       aggcagCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATtgtg                                                                                                                                         >  8:742079/1‑147 (MQ=255)
       aggcagCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATtgtg                                                                                                                                         <  7:742079/147‑1 (MQ=255)
         gcagCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATtgtg                                                                                                                                         <  4:437385/145‑1 (MQ=255)
         gcagCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATtgtg                                                                                                                                         >  3:437385/1‑145 (MQ=255)
         gcagCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATtgt                                                                                                                                          >  8:70451/1‑144 (MQ=255)
         gcagCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATtgt                                                                                                                                          <  7:70451/144‑1 (MQ=255)
         gaagCGAAACCGTGTGACAGGTTACCGCATGACAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGTTAAGCAGAGCGAGTCTCGCGGGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGAtt                                                                                                                                     <  6:391651/147‑1 (MQ=255)
            gCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCAGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCa                                                                                                                                   >  3:162422/1‑148 (MQ=255)
              gAAACCGTGTGCCAGGTTACAGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTAGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAAAGATTCGATTTGTTACGCGCAACAATTGTGGATTCATa                                                                                                                                 >  7:454245/1‑148 (MQ=255)
                aaCCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAg                                                                                                                              <  6:552923/149‑1 (MQ=255)
                  ccGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGc                                                                                                                            >  8:647875/1‑149 (MQ=255)
                   cGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCt                                                                                                                           >  1:526275/1‑149 (MQ=255)
                        gCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTg                                                                                                                       >  6:581891/1‑148 (MQ=255)
                          cAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCACGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGaaa                                                                                                                    >  8:754797/1‑149 (MQ=255)
                           aGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAAt                                                                                                                   >  6:154053/1‑149 (MQ=255)
                              ttACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAACGGCTCCTGAAATTGa                                                                                                                <  3:217504/149‑1 (MQ=255)
                              ttACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAAtt                                                                                                                  >  5:629478/1‑147 (MQ=255)
                              ttACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGa                                                                                                                <  7:647875/149‑1 (MQ=255)
                                aCCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAg                                                                                                               <  3:63871/148‑1 (MQ=255)
                                  cGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTg                                                                                                            >  7:488730/1‑149 (MQ=255)
                                    cATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTc                                                                                                          <  2:78439/149‑1 (MQ=255)
                                    cATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTc                                                                                                          <  1:271836/149‑1 (MQ=255)
                                    cATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTc                                                                                                          <  3:252026/149‑1 (MQ=255)
                                         cAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAg                                                                                                                              >  2:62274/1‑124 (MQ=255)
                                         cAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGa                                                                                                                <  7:111664/138‑1 (MQ=255)
                                         cAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCagag                                                                                                      >  6:227693/1‑148 (MQ=255)
                                           aCTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGATCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACTATTGTGGATTCTTAATGGCTCCTGAAATTGAGTTGTCAGAGCAg                                                                                                   <  4:219933/149‑1 (MQ=255)
                                           aCTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATaa                                                                                                                                >  8:450753/1‑120 (MQ=255)
                                                 aaCGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCATAGCAGgatgat                                                                                             <  2:397591/149‑1 (MQ=255)
                                                  aCGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGAtt                                                                                            <  5:154053/149‑1 (MQ=255)
                                                    gATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTca                                                                                          >  4:457165/1‑149 (MQ=255)
                                                     aTGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTca                                                                                          >  1:34957/1‑148 (MQ=255)
                                                      tGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGgatgataga                                                                                          >  1:115505/1‑144 (MQ=255)
                                                      tGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGgatga                                                                                              <  2:115505/143‑1 (MQ=255)
                                                          cGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAAcgc                                                                                    <  8:500312/149‑1 (MQ=255)
                                                              cGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGttt                                                                                >  8:335684/1‑149 (MQ=255)
                                                               gAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTttc                                                                               <  8:646345/149‑1 (MQ=255)
                                                                   ttttCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTttcatt                                                                            >  5:476256/1‑148 (MQ=255)
                                                                   ttttCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTttcatt                                                                            >  4:360332/1‑148 (MQ=255)
                                                                       cGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCaga                                                                        <  1:770245/148‑1 (MQ=255)
                                                                        gCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCTAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTgg                                                                                                                                        <  3:551100/83‑1 (MQ=255)
                                                                        gCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCagag                                                                       >  8:541495/1‑148 (MQ=255)
                                                                        gCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCAAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTgg                                                                                                                                        >  4:551100/1‑83 (MQ=255)
                                                                            gTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCATGATGATTCACAACGCGTTTCATTCAGAGGAtt                                                                   <  1:407663/148‑1 (MQ=255)
                                                                               cTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTAt                                                                <  7:381537/148‑1 (MQ=255)
                                                                                 aTCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGAc                                                             >  2:580765/1‑149 (MQ=255)
                                                                                  tCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGa                                                                     >  7:746305/1‑140 (MQ=255)
                                                                                  tCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACt                                                            <  3:242328/149‑1 (MQ=255)
                                                                                  tCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACt                                                            >  1:335630/1‑149 (MQ=255)
                                                                                  tCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACCTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACt                                                            >  8:243781/1‑149 (MQ=255)
                                                                                  tCCGGGCGAGATAAGAAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGCTTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGAc                                                             <  2:429403/148‑1 (MQ=255)
                                                                                      ggCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGaaa                                                        <  7:154460/149‑1 (MQ=255)
                                                                                       gCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAAtt                                                                                                                  <  7:220193/90‑1 (MQ=255)
                                                                                       gCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAAtt                                                                                                                  >  8:220193/1‑90 (MQ=255)
                                                                                         gagaTAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGATCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGc                                                     <  4:162422/149‑1 (MQ=255)
                                                                                         gagaTAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGc                                                     <  1:711047/149‑1 (MQ=255)
                                                                                          agaTAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGcc                                                    <  5:581891/149‑1 (MQ=255)
                                                                                           gaTAAGCAGTGCGAGTCTGGCGCGAACGTTCATTGATTAGATTTGTTACGCGCAACAATTGTGGATTCTTAAAGGCTCCTGAATTTGTGTTGTCTGAGGAGGATGATTCACTACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCt                                                   <  4:125728/149‑1 (MQ=255)
                                                                                           gaTAAGCAGCGCGAGTCTCGCGCGAACGTTCAATGCTTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAAGTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATGTATGACTGAAACGCCt                                                   >  2:302897/1‑149 (MQ=255)
                                                                                           gaTAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCt                                                   <  1:230084/149‑1 (MQ=255)
                                                                                             tAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGt                                                 <  7:541495/149‑1 (MQ=255)
                                                                                               aGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTa                                                                 >  8:3762/1‑131 (MQ=255)
                                                                                                 cAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAAcc                                             <  2:526275/149‑1 (MQ=255)
                                                                                                     tcGAGTCTCGCGCGAACGTTCAATGATTCTATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAg                                         <  6:470822/148‑1 (MQ=255)
                                                                                                           ctcGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGa                                          >  1:618832/1‑142 (MQ=255)
                                                                                                            tcGCGCGAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGTAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAg                                  >  7:77153/1‑149 (MQ=255)
                                                                                                              gcgcgAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGATATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAg                                  <  1:580765/147‑1 (MQ=255)
                                                                                                              gcgcgAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATa                                   <  2:729881/146‑1 (MQ=255)
                                                                                                                 cgAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCa                              >  1:185877/1‑148 (MQ=255)
                                                                                                                  gAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGaaa                                                        <  8:597340/121‑1 (MQ=255)
                                                                                                                  gAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGaaa                                                        >  7:597340/1‑121 (MQ=255)
                                                                                                                  gAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCaa                             >  6:355155/1‑148 (MQ=255)
                                                                                                                  gAACGTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACGGAAACGCCTGTAACCGGAGCTCATAGGGCaaa                            <  3:360332/149‑1 (MQ=255)
                                                                                                                      gTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTttcattca                                                                          <  5:195774/99‑1 (MQ=255)
                                                                                                                      gTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGAt                                                                    >  5:277012/1‑105 (MQ=255)
                                                                                                                      gTTCAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGAt                                                                    <  6:277012/105‑1 (MQ=255)
                                                                                                                         cAATGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGtt                      <  3:127136/148‑1 (MQ=255)
                                                                                                                          aaTGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGttt                     <  2:185877/148‑1 (MQ=255)
                                                                                                                           aTGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGttt                     >  4:96323/1‑147 (MQ=255)
                                                                                                                           aTGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTtca                   <  2:247221/149‑1 (MQ=255)
                                                                                                                           aTGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCACGATGATTCACAACGCGTTTCATTCAGAGGATTTATGAATGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGtat                     >  5:709387/1‑145 (MQ=255)
                                                                                                                            tGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGAtt                                                                                            <  6:689867/75‑1 (MQ=255)
                                                                                                                            tGATTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGAtt                                                                                            >  5:689867/1‑75 (MQ=255)
                                                                                                                              aTTCGATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTtcagt                 <  8:464116/148‑1 (MQ=255)
                                                                                                                                  gATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTcagtcagt             <  7:335684/148‑1 (MQ=255)
                                                                                                                                  gATTTGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTcagtcagt             <  5:306029/148‑1 (MQ=255)
                                                                                                                                    tttGTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTtca                   >  1:341782/1‑140 (MQ=255)
                                                                                                                                       gTTACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGTCAAAAACGTTTCAGTCAGTGtaat        >  8:115975/1‑148 (MQ=255)
                                                                                                                                        ttACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGtaata       >  6:629394/1‑148 (MQ=255)
                                                                                                                                        ttACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGtaata       >  3:86111/1‑148 (MQ=255)
                                                                                                                                        ttACGCGCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGtaata       >  4:250517/1‑148 (MQ=255)
                                                                                                                                           cgcgCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATaaaaa   <  7:337649/149‑1 (MQ=255)
                                                                                                                                           cgcgCAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACAGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATaaaa    >  6:175499/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
GGCTGGCAGGCAGCGAAACCGTGTGCCAGGTTACCGCATGCCAACTGCGAACGATGAACGGTCGAAGTTTTCGCTTGTTCTATCCGGGCGAGATAAGCAGAGCGAGTCTCGCGCGAACGTTCAATGATTCGATTTGTTACGCGTAACAATTGTGGATTCATAAAGGCTCCTGAAATTGAGTTGTCAGAGCAGGATGATTCACAACGCGTTTCATTCAGAGGATTTATGACTGAAACGCCTGTAACCGGAGCTCATAGGGCAAAAACGTTTCAGTCAGTGTAATAAAAAA  >  NC_000913/1934444‑1934732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: