Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC JC	NC_000913	3,463,556	IS2 (+) +5 bp	35.4%	coding (633‑637/984 nt)	gspK →	general secretory pathway component, cryptic

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3186096 =	NA (NA)	25 (0.290)	21/264	NT	31.6%	noncoding (1/1331 nt)	IS2	repeat region
	?	NC_000913	= 3463560	52 (0.620)					coding (637/984 nt)	gspK	general secretory pathway component, cryptic
*	?	NC_000913	= 3187426	NA (NA)	32 (0.370)	27/264	NT	37.2%	noncoding (1331/1331 nt)	IS2	repeat region
	?	NC_000913	3463556 =	52 (0.620)					coding (633/984 nt)	gspK	general secretory pathway component, cryptic

CAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3187291‑3187426 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCTCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTT  >  NC_000913/3463556‑3463703                                                                                                                                                                                                                                                                                              CAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACACGGAACTTAGC                                                                                                                                         <  8:377546/149‑1  AGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTATTGGGTTAAGTGATATCAGATGTCTGGATATATAGGGGCTAATCCACCCGGAACTTAGCA                                                                                                                                        >  2:98819/1‑149  AGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGATCGCAACAGGAATATCTGCGGCAGCGGGCTTGTAATGGGTGAAGTGATAACAGATGTCTGTAAATATAGGGGCAAATCCACACGGAACTTAGCA                                                                                                                                        >  7:272567/1‑149            GAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACACGGAACTTAGCATCAATCTTAA                                                                                                                              >  2:285694/1‑149            GAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACACGGAACTTAGCATCAATCTTAA                                                                                                                              <  5:319262/149‑1             AGCATTATAACGAATGGCATCCGCATAGTTCGCTGGGTTATCGCTCGCCACAGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACACGGAACTTAGCATCAATCTTAAT                                                                                                                             <  8:272567/149‑1             AGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACACGGAACTTAGCATCAATCTTAAT                                                                                                                             <  2:386206/149‑1               CATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACACGGAACTTAGCATCAATCTTAATAT                                                                                                                           <  4:336354/149‑1                     AACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACACGGAACTTAGCATCAATCTTAATATGCTAAC                                                                                                                     >  4:246511/1‑149                           TGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAA                                                                                                               <  7:316859/149‑1                                   tCATAGTGAGCTGGGTTATCGCTCGCAACGGTAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTTATAACAGATGTCTGGAAATATAGGGGCAAATACACAAGGAACTTAGCATCAATATTAATATGCTAACGGAGAACTATATTC                                                                                                       >  8:64733/2‑149                                   GCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTC                                                                                                       <  4:339335/149‑1                                           CGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTT                                                                                                <  1:285694/148‑1                                                       GCTCGCGACGGGAATATCTGCGGCAGCTTGCTTGTAATGGGTTAAGTGTTAACAGATGTCTGGAAATATAGGGGCAAATCCACACGGAACTTAGCATAAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTA                                                                                      <  1:246510/146‑1                                                       GCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGTGGCAAATCCACACGGAACTTAGAATCAATCTTAATATGCTAACGGAGAACGATATTCCGCACTTCAGAGCGATA                                                                                      >  2:246510/1‑146                                                                       TCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACG                                                                    <  3:246511/148‑1                                                                                 CGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCATCAATAACATCACGGATGCTGACGC                                                         >  3:54523/1‑149                                                                                      TTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCTCGGG                                                    >  7:48304/1‑149                                                                                            TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCTCGGGTCTTAT                                              >  7:22560/1‑149                                                                                            TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCTCGGGTCTTAT                                              >  5:325261/1‑149                                                                                            TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGAT                                                                 >  5:206238/1‑130                                                                                            TGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGAT                                                                 <  6:206238/130‑1                                                                                                TTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACATTATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCTCGGGTCTTATTGC                                           >  7:190027/1‑148                                                                                                 TAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCTCGGGTCTTATTGCA                                          >  8:129167/1‑148                                                                                                       ATAACAGATGTCTGGAAATATAGGGGCAAATCCACACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCTCGGGTCTTATTGCAAAAAAtg                                   >  5:365270/1‑147                                                                                                          ACAGATGTCTGGAAATATAGGGGCAAATCCACACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCTCGGGTCTTATTGCAAAAAAGGCC                                 >  7:252593/1‑148                                                                                                            AGATGTCTGGAAATATAGGGGCAAATCCACACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCTCGGGTCTTATTGCAAAAAAGGCCGAG                              >  1:334266/1‑149                                                                                                                   TGGAAATATAGGGGCAAATCCACACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCTCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGC                       >  1:323617/1‑149                                                                                                                      AAATATAGGGGCAAATCCACACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGAAGCTCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGG                    >  5:291123/1‑149                                                                                                                        ATATAGGGGCAAATCCACACGGAACTTATCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCTCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCT                  <  5:133365/149‑1                                                                                                                        ATATAGGGGCAAATCCACACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCTCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGC                   >  3:329971/1‑148                                                                                                                           aAGGGGCAAATCCACACGGAACTTAGCAGCAATCTTAATATGCTAACGGATAACGATATTCCGCTATTCAGAGCGTTATTCCTCAATAACATCACTGATGCTGACGCTCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAAC               <  1:98819/148‑1                                                                                                                                        ACACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCTCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTT  <  8:22560/149‑1                                                                                                                                        ACACGGAACTTAGCATCAATATTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGTATGCTGACGCTCGTGTCTTATTGCAAAAAAGGCAGAGGGAATGCTGGCTAACCACGGATCCATTT  >  5:16514/1‑149                                                                                                                                                                                                                                                                                              CAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3187291‑3187426 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CACGGAACTTAGCATCAATCTTAATATGCTAACGGAGAACGATATTCCGCTCTTCAGAGCGTTATTCCTCAATAACATCACGGATGCTGACGCTCGGGTCTTATTGCAAAAAAGGCCGAGGGAAGGCTGGCTAACCACGGATGCATTT  >  NC_000913/3463556‑3463703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.