New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 3786857134 (1.550)3 (0.040) 3/254 NT 2.3% coding (20/1260 nt) envC activator of AmiB,C murein hydrolases, septal ring factor
?NC_000913 = 3786862 130 (1.560)coding (25/1260 nt) envC activator of AmiB,C murein hydrolases, septal ring factor
Rejected: Frequency below/above cutoff threshold.

GGCGGCAAACTTTCTGACATCGCGCCGACCATGTTGTCGCTGATGGGTATGGAAATCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCG‑‑TGAAACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3786706‑3786857
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aaaccTGCGCGGTTTCACGGCCCGTGTCATGGTATTAATCGCCTTTCCCCTCATGGGGAGGGATTATTCCACGATGAACAGCGGCTTACCAGTCATCTCTTGCGGGATTTCCATACCCATCAGCGACAACATGGTCGGCGCGATGTCAGAAA  <  NC_000913/3786862‑3786716
                                                                                                                                                                                                                                                                                                                
GGCGGCAAACTTTCTCACATCGCGCCGACCATGTTGTCGCTGATGGGTATGCAAATCCCGCAATAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGCGGGGAAAGGCGATTAATACCATGACACGGGCCG‑‑TGA                                                                                                                                                          >  1:211505/1‑148
 GCGGCAAACTTTCTGACATCGCGCCGACCATGTTGTCGCTGATGGGTATGGAAATCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCG‑‑TGAAA                                                                                                                                                        <  5:56247/149‑1
   GGCAAACTTTCTGACATCGCGCCGACCATGTTGTCGCTGATGGGTATGGAAATCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCG‑‑TGAAACC                                                                                                                                                      >  5:334111/1‑149
   GGCAAACTTTCTGACATCGCGCCGACCATGTTGTCGCTGATGGGTATGGAAATCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCG‑‑TGAAACC                                                                                                                                                      >  6:248827/1‑149
                         CGACCATGTTGTCGCTGATGGGTATGGAAATCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCTGATTGCAAACCTGCGCGGTTTCACGGCCC                                                                                                                                   <  1:51090/149‑1
                                                                                       ATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCG‑‑TGAAACCGCGCAGGTTTCACGGCCCGTGTCATGGTATTAATCGCCTTTCCCC                                                                                                        <  3:305191/110‑1
                                                                                       ATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCG‑‑TGAAACCGCGCAGGTTTCACGGCCCGTGTCATGGTATTAATCGCCTTTCCCC                                                                                                        >  4:305191/1‑110
                                                                                                                                                          CCTGCGCGGTTTCACGGCCCGTGTCATGGTATTAATCGCCTTTCCCCTCATGGGGAGGGATTATTCCACGATGAACAGCGGCTTACCAGTCATCTCTTGCGGGATTTCCATACCCATCAGCGACAACATGGTCGGCGCGATGTCAGAAA  <  1:40120/149‑1
                                                                                                                                                                                                                                                                                                                
GGCGGCAAACTTTCTGACATCGCGCCGACCATGTTGTCGCTGATGGGTATGGAAATCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCG‑‑TGAAACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3786706‑3786857
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aaaccTGCGCGGTTTCACGGCCCGTGTCATGGTATTAATCGCCTTTCCCCTCATGGGGAGGGATTATTCCACGATGAACAGCGGCTTACCAGTCATCTCTTGCGGGATTTCCATACCCATCAGCGACAACATGGTCGGCGCGATGTCAGAAA  <  NC_000913/3786862‑3786716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.