New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3880427 =143 (1.650)3 (0.040) 3/254 NT 2.1% coding (795/1074 nt) recF gap repair protein
?NC_000913 3880485 = 145 (1.740)coding (737/1074 nt) recF gap repair protein
Rejected: Frequency below/above cutoff threshold.

CTCTGACTTTCTCTTTCCAGCGCGGCTGGGAGAAAGAGACAGAATATGCTGAGGTGCTGGAACGTAATTTTGAACGCGATCGCCAGCTAACCTACACCGCGCACGGCCCGCACAAAGCGGACTTACGCATTCGCGCCGACGGTGCGCCGGTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3880578‑3880427
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cggtgTAGGTTAGCTGGCGATCGCGTTCAAAATTACGTTCCAGCACCTCAGCATATTCTGTCTCTTTCTCCCAGCCGCGCTGGAAAGAGAAAGTCAGAGAAAACTCAGGGAGAAATTGCTTACAGGTATCAGCCATATCGGCCGCGATACCGG  >  NC_000913/3880485‑3880632
                                                                                                                                                                                                                                                                                                            
CTCTGACTTTCTCTTTCCAGCGCGGCTGGGAGAAAGAGACAGAATATGCTGAGGTGCTGGAACGTAATTTTGAACGCGATCGCCAGCTAACCTACACCGCGCACGGCCCGCACAAAGCGGACTTACGCATTCGCGCCGACGGTGCGCCG                                                                                                                                                         >  1:22385/1‑149
   TGACTTTCTCTTTCCAGCGCGGCTGGGAGAAAGAGACAGAATATGCTGAGGTGCTGGAACGTAATTTTGAACGCGATCGCCAGCTAACCTACACCGCGCACGGCCCGCACAAAGCGGACTTACGCATTCGCGCCGACGGTGCGCCGGTG                                                                                                                                                      >  6:84982/1‑149
   TGACTTTCTCTTTCCAGCGCGGCTGGGAGAAAGAGACAGAATATGCTGAGGTGCTGGAACGTAATTTTGAACGCGATCGCCAGCTAACCTACACCGCGCACGGCCCGCACAAAGCGGACTTACGCATTCGCGCCGACGGTGCGCCGGTG                                                                                                                                                      <  6:35448/149‑1
   TGACTTTCTCTTTCCAGCGCGGCTGGGAGAAAGAGACAGAATATGCTGAGGTGCTGGAACGTAATTTTGAACGCGATCGCCAGCTAACCTACACCGCGCACGGCCCGCACAAAGCGGACTTACGCATTCGCGCCGACGGTGCGCCGG                                                                                                                                                        <  1:304719/147‑1
     ACTTTCTCTTTCCAGCGCGGCTGGGAGAAAGAGACAGAATATGCTGAGGTGCTGGAACGTAATTTTGAACGCGATCGCCAGCTAACCTACACCGCGCACGGCCCGCACAAAGCGGACTTACGCATTCGCGCCGACGGTGCGCCGGT                                                                                                                                                       <  4:200632/146‑1
       TTTCTCTTTCCAGCGCGGCTGGGAGAAAGAGACAGAATATGCTGAGGTGCTGGAACGTAATTTTGAACGCGATCGCCAGCTAACCTACACCGCGCACGGCCCGCACAAAGCGGACTTACGCATTCGCGCCGACGGTGCGCCGGagg                                                                                                                                                     >  5:324713/1‑143
                                                                                       TAACCTACACCGCGCACGGCCCGCACAAAGCGGACTTACGCATTCGCGCCGACGGTGCGCCGGTGTAGGTTAGCTGGCGATCGCGTTCAAAATTACGTTCCAGCACCTCAGCATATTCTGT                                                                                              <  3:346149/121‑1
                                                                                       TAACCTACACCGCGCACGGCCCGCACAAAGCGGACTTACGCATTCGCGCCGACGGTGCGCCGGTGTAGGTTAGCTGGCGATCGCGTTCAAAATTACGTTCCAGCACCTCAGCATATTCTGT                                                                                              >  4:346149/1‑121
                                                                                                 CGCGCACGGCCCGCACAAAGCGGACTTAAGCATTCGCGCCGACGGTGCGCCGGTGTAGGTTAGCTGGCGATCGCGTTCAAAATTACGTTCCAGCACCTCAGCATATTCTGTCTCTTTCTCCCAGCCGCGCTGGAAAGAGAAAGTCAGAG                                                        <  3:36766/149‑1
                                                                                                                                                 cgctGTGTAGGTTAGCTGGCGATCGCGTTCAAAATTACGTTCCAGCACCTCAGCATATTCTGTCTCTTTCTCCCAGCCGCGCTGGAAAGAGAAAGTCAGAGAAAACTCAGGGAGAAATTGCTTACAGGTATCAGCCATATCGGCCGCGA        <  5:198017/145‑1
                                                                                                                                                   CGGTGTAGGTTAGCTGGCGATCGCGTTCAAAATTACGTTCCAGCACCTCAGCATATTCTGTCTCTTTCTCCCAGCCGCGCTGGAAAGAGAAAGTCAGAGAAAACTCAGGGAGAAATTGCTTACAGGTATCAGCCATATCGGCCGCGATA      <  3:200632/149‑1
                                                                                                                                                    GGTGTAGGTTAGCTGGCGATCGCGTTCAAAATTACGTTCCAGCACCTCAGCATATTCTGTCTCTTTCTCCCAGCCGCGCTGGAAAGAGAAAGTCAGAGAAAACTCAGGGAGAAATTGCTTACAGGTATCAGCCATATCGGCCGCGATAC     <  5:286134/149‑1
                                                                                                                                                    GGTGTAGGTTAGCTGGCGATCGCGTTCAAAATTACGTTCCAGCACCTCAGCATATTCTGTCTCTTTCTCCCAGCCGCGCTGGAAAGAGAAAGTCAGAGAAAACTCAGGGAGAAATTGCTTACAGGTATCAGCCATATCGGCCGCGATAC     >  5:118407/1‑149
                                                                                                                                                     GTGTAGGTTAGCTGGCGATCGCGTTCAAAATTACGTTCCAGCCCCTCAGCATATTCTGTCTCTTTCTCCCAGCCGCGCTGGAAAGAGAAAGTCAGAGAAAACTCAGGGAGAAATTGCTTACAGGTATC                         >  4:262314/1‑128
                                                                                                                                                     GTGTAGGTTAGCTGGCGATCGCGTTCAAAATTACGTTCCAGCACCTCAGCATATTCTGTCTCTTTCTCCCAGCCGCGCTGGAAAGAGAAAGTCAGAGAAAACTCAGGGAGAAATTGCTTACAGGTATC                         <  3:262314/128‑1
                                                                                                                                                     GTGTAGGTTAGCTGGCGATCGCGTTCAAAATTACGTTCCAGCACCTCAGCATATTCTGTCTCTTTCTCCCAGCCGCGCTGGAAAGAG                                                                  >  4:300300/1‑87
                                                                                                                                                     GTGTAGGTTAGCTGGCGATCGCGTTCAAAATTACGTTCCAGCACCTCAGCATATTCTGTCTCTTTCTCCCAGCCGCGCTGGAAAGAG                                                                  <  3:300300/87‑1
                                                                                                                                                      TGTAGGTTAGCTGGCGATCGCGTTCAAAATTACGTTCCAGCACCTCAGCATATTCTGTCTCTTTCTCCCAGCCGCGCTGGAAAGAGAAAGTCAGAGAAAACTCAGGGAGAAATTGCTTACAGGTATCAGCCATATCGGCCGCGATACCG   >  4:105292/1‑149
                                                                                                                                                       GTAGGTTAGCTGGCGATCGCGTTCAAAATTACGTTCCAGCACCTCAGCATATTCTGTCTCTTTCTCCCAGCCGCGCTGGAAAGAGAAAGTCAGAGAAAACTCAGGGAGAAATTGCTTACAGGTATCAGCCATATCGGCCGCGATACCGG  >  3:25521/1‑149
                                                                                                                                                       GTAGGTTAGCTGGCGATCGCGTTCAAAATTACGTTCCAGCACCTCAGCATATTCTGTCTCTTTCTCCCAGCCGCGCTGGAAAGAGAAAGTCAGAGAAAACTCAGGGAGAAATTGCTTACAGGTATCAGCCATATCGGCCGCGATACCGG  <  7:334780/149‑1
                                                                                                                                                                                                                                                                                                            
CTCTGACTTTCTCTTTCCAGCGCGGCTGGGAGAAAGAGACAGAATATGCTGAGGTGCTGGAACGTAATTTTGAACGCGATCGCCAGCTAACCTACACCGCGCACGGCCCGCACAAAGCGGACTTACGCATTCGCGCCGACGGTGCGCCGGTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3880578‑3880427
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cggtgTAGGTTAGCTGGCGATCGCGTTCAAAATTACGTTCCAGCACCTCAGCATATTCTGTCTCTTTCTCCCAGCCGCGCTGGAAAGAGAAAGTCAGAGAAAACTCAGGGAGAAATTGCTTACAGGTATCAGCCATATCGGCCGCGATACCGG  >  NC_000913/3880485‑3880632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.