Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,261,474	C→A	100%	Q134H (CAG→CAT)	prs ←	phosphoribosylpyrophosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,261,474	0	C	A	100.0%	221.9 / NA	66	Q134H (CAG→CAT)	prs	phosphoribosylpyrophosphate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (30/36);  total (30/36)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGATAACAG  >  NC_000913/1261330‑1261616                                                                                                                                                 |                                                                                                                                               cTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGtt                                                                                                                                            >  1:128923/1‑149 (MQ=255)    aGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCa                                                                                                                                          <  6:155854/148‑1 (MQ=255)    aGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAg                                                                                                                                         >  2:240246/1‑149 (MQ=255)     gCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGc                                                                                                                                        <  2:128923/149‑1 (MQ=255)      cGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCg                                                                                                                                       <  1:285516/149‑1 (MQ=255)       gATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTCGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGt                                                                                                                                      >  5:91323/1‑149 (MQ=255)         tAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGGTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCATCGTGc                                                                                                                                    <  3:155895/149‑1 (MQ=255)               gggCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATcc                                                                                                                              <  5:320182/149‑1 (MQ=255)                     gcacAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATg                                                                                                                                              >  3:125872/1‑127 (MQ=255)                     gcacAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATg                                                                                                                                              <  4:125872/127‑1 (MQ=255)                     gcacAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTc                                                                                                                        <  8:22920/149‑1 (MQ=255)                     gcacAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTAGCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTc                                                                                                                        >  5:43132/1‑149 (MQ=255)                      cacaACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCa                                                                                                                       <  6:72979/149‑1 (MQ=255)                         aaCGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAg                                                                                                                      >  7:219573/1‑147 (MQ=255)                         aaCGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAg                                                                                                                      <  8:219573/147‑1 (MQ=255)                           cgccgcCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGcaca                                                                                                                  <  1:45534/149‑1 (MQ=255)                                ccgATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGt                                                                                                              >  2:275114/1‑148 (MQ=255)                                acgATGTCCTGAGAAAGCACAATTTGGTTATACAGATTCAGCTGCAGCATGTCTTCCAGAAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCTTGCAGATCCACTGTCAGCACACGGTc                                                                                                             <  3:240479/148‑1 (MQ=255)                                  gATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAACCCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCaa                                                                                                           >  5:333449/1‑149 (MQ=255)                                  gATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCaa                                                                                                           >  5:29266/1‑149 (MQ=255)                                      tCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGGGCAGATCCACTGTCAGCACACGGGCAacac                                                                                                        <  8:297376/148‑1 (MQ=255)                                          gagaAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACg                                                                                                   >  4:74074/1‑149 (MQ=255)                                          gagaAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACg                                                                                                   >  4:69043/1‑149 (MQ=255)                                                ccACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTg                                                                                                >  3:42424/1‑146 (MQ=255)                                                ccACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGga                                                                                              <  4:42424/148‑1 (MQ=255)                                                 cacaATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGgaga                                                                                            <  6:333449/149‑1 (MQ=255)                                                  acaATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGagag                                                                                           >  7:135874/1‑149 (MQ=255)                                                    aaTTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAgg                                                                                          >  5:92382/1‑148 (MQ=255)                                                           ttATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAAGAGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGTAAGTCTGc                                                                                  <  4:155655/149‑1 (MQ=255)                                                           ttATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACTGTCAACACCGACGCTGGAGAGGAAGTCTGc                                                                                  <  1:275114/149‑1 (MQ=255)                                                              tCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATAAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAAc                                                                               >  5:4009/1‑149 (MQ=255)                                                               ccAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAAc                                                                               >  3:265995/1‑148 (MQ=255)                                                                      cAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACAGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTc                                                                        <  1:105688/148‑1 (MQ=255)                                                                        gCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAACCCCTTAAAATGTGAAGCTTGAAGATCCACGTTCAGCACCCGGTCAACACGCACGCTGGAGCGGAAGGATGCAACCAATTTCg                                                                       >  5:316591/1‑147 (MQ=255)                                                                        gCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCa                                                                     <  6:29266/149‑1 (MQ=255)                                                                         cTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGgaga                                                                                            <  4:336015/125‑1 (MQ=255)                                                                         cTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGgaga                                                                                            >  3:336015/1‑125 (MQ=255)                                                                            cagcaTGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGt                                                                   <  8:135874/147‑1 (MQ=255)                                                                             agcaTGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGAt                                                                <  1:72345/149‑1 (MQ=255)                                                                              gcaTGGCTACCAGCTGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGTAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACTGTCAACACCGACGCTGGAGTGGAAGTCTGCAACCACTATCGAAGTGAtt                                                               <  5:108112/149‑1 (MQ=255)                                                                               caTGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGc                                                                                                                                        >  5:90684/1‑75 (MQ=255)                                                                               caTGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGc                                                                                                                                        <  6:90684/75‑1 (MQ=255)                                                                               caTGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTg                                                              >  5:358696/1‑149 (MQ=255)                                                                                   tCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTa                                                           <  6:322802/148‑1 (MQ=255)                                                                                                tCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGAtt                                                               <  2:211704/131‑1 (MQ=255)                                                                                                tCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGAtt                                                               >  1:211704/1‑131 (MQ=255)                                                                                                  gggCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGAc                                           <  3:69043/149‑1 (MQ=255)                                                                                                  gggCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGAc                                           <  3:74074/149‑1 (MQ=255)                                                                                                   ggCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGAcg                                          <  3:107024/149‑1 (MQ=255)                                                                                                     cTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCAGAACGGAcgcg                                        <  4:213820/149‑1 (MQ=255)                                                                                                      tACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGa                                       >  6:343751/1‑149 (MQ=255)                                                                                                         cAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACgg                                    >  4:20328/1‑149 (MQ=255)                                                                                                           aattagTTATCAACCGGGAATGCGAACAAACCCTGAATATGTTCAGCGGGCAGATCCACTGTCAGCACACGGTCATCACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTc                                  <  6:316591/144‑1 (MQ=255)                                                                                                            aTACGTTATCAACCGGAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTcc                                 >  1:95267/1‑149 (MQ=255)                                                                                                                           gAACGTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGcc                   >  5:68385/1‑148 (MQ=255)                                                                                                                               gTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAg               >  4:223885/1‑148 (MQ=255)                                                                                                                               gTCGAAGAAACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGt              <  5:343751/149‑1 (MQ=255)                                                                                                                                 cGAAGAAACCCTTAATATGTTCAGCGTGCAGATCCTCTGTCTGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGAGGAACGGACGCGACTGTCCTGGCGCGCATAGCCAAAGTAg            <  6:4009/149‑1 (MQ=255)                                                                                                                                  gaagaaACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAgg           <  6:92382/149‑1 (MQ=255)                                                                                                                                   aagaaACCCTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAggg          >  7:375237/1‑149 (MQ=255)                                                                                                                                    agaaACCCTGAATATGTTCAGCGTGTAGATCCACTGTCAGCACACGTTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAggg          <  6:255455/148‑1 (MQ=255)                                                                                                                                         cccTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGAGaa     >  8:296023/1‑148 (MQ=255)                                                                                                                                         cccTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACAGTCAACACCGACGCTGGAGAGGAAGTCTGCACCCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGATaa     >  3:335179/1‑148 (MQ=255)                                                                                                                                          ccTTAATATGTTCAGCGTTCAGATCCACTGTCAGCACACGGGCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATa                      <  6:299758/130‑1 (MQ=255)                                                                                                                                          ccTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATa                      >  5:299758/1‑130 (MQ=255)                                                                                                                                           cTGAATATGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCGGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGATAACAg  <  1:240246/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                               CTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGATAACAG  >  NC_000913/1261330‑1261616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑