Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,275,577 C→T 5.4% A102V (GCT→GTT)  yejE → microcin C ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,275,5770CT 5.4% 241.4 / 2.6 74A102V (GCT→GTT) yejEmicrocin C ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (28/42);  new base T (2/2);  total (30/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGTTGGTATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGA  >  NC_000913/2275430‑2275723
                                                                                                                                                   |                                                                                                                                                  
gCAGTTGGTATTTCCCGTTATTGAAAAACTACCGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGGCAGGCTGCTTATCAGGACCCGGGGCTGAAACAACGGCTGGAAAATAACGGCTGGGGACTGTGGGCACCGATTCGCTTTGGTGCt                                                                                                                                                   <  1:313712/149‑1 (MQ=255)
  aGTTGGTATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTAc                                                                                                                                                 <  5:276470/149‑1 (MQ=255)
    ttGGTATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGTCTGGGTACTGTGTGCACCGATTCGCTTTTTTGCTACCa                                                                                                                                               <  4:37591/149‑1 (MQ=255)
    ttGGTATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTAc                                                                                                                                                 >  6:162741/1‑147 (MQ=255)
    ttGGTATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCa                                                                                                                                               <  8:165857/149‑1 (MQ=255)
    ttGGTATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCa                                                                                                                                               <  6:315315/149‑1 (MQ=255)
       gTATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGt                                                                                                                                             <  7:7322/148‑1 (MQ=255)
        tATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTAt                                                                                                                                           <  8:278973/149‑1 (MQ=255)
         aTTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTAt                                                                                                                                           >  4:208560/1‑148 (MQ=255)
            tCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCaa                                                                                                                                        <  8:200826/148‑1 (MQ=255)
                gTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTg                                                                                                                                   >  1:303595/1‑149 (MQ=255)
                 ttATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGc                                                                                                                                  <  5:274608/149‑1 (MQ=255)
                 ttATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGCTACTGTGGGCACCGCTTCGCTTTGGTGCTACCAGTATCAACTTTGc                                                                                                                                  >  6:202037/1‑149 (MQ=255)
                    ttGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTa                                                                                                                                <  7:315119/148‑1 (MQ=255)
                    ttGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTAc                                                                                                                               >  5:92233/1‑149 (MQ=255)
                    ttGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTAc                                                                                                                               <  2:303595/149‑1 (MQ=255)
                      gAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTAcc                                                                                                                              >  4:127562/1‑148 (MQ=255)
                       aaaaaCTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCaa                                                                                                                            <  6:92233/149‑1 (MQ=255)
                            cTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAACTCAGGCTGATTATCAGGACCCGTGGCGGAAACAACGGCTGGAAAATACCGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGc                                                                                                                       <  5:318152/149‑1 (MQ=255)
                                aGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGccctt                                                                                                                   <  1:294419/149‑1 (MQ=255)
                                   gAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGcccttccc                                                                                                                <  3:206463/149‑1 (MQ=255)
                                    aaaGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGTTACCAGTATCAACTTTGCTACCAATAAGcccttccct                                                                                                               >  7:363736/1‑149 (MQ=255)
                                      aGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCccttcccttc                                                                                                             <  7:369078/149‑1 (MQ=255)
                                        cGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTtctc                                                                                                           <  7:149406/149‑1 (MQ=255)
                                             ttGGCGGCCCGCTGGCAAGTCAGGCTGATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCAcc                                                                                                       <  2:183162/148‑1 (MQ=255)
                                                   gcCCGCTGGCAAGTCAGGCTGATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCgg                                                                                                <  3:183033/149‑1 (MQ=255)
                                                    cccGCTGGCAAGTCAGGCTGATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCgg                                                                                                >  2:380668/1‑148 (MQ=255)
                                                     ccGCTGGCAAGTCAGGCTGATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGc                                                                                               >  1:378929/1‑148 (MQ=255)
                                                        cTGGCAAGTCAGGCTGATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAAt                                                                                                                           >  8:256802/1‑117 (MQ=255)
                                                        cTGGCAAGTCAGGCTGATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAAt                                                                                                                           <  7:256802/117‑1 (MQ=255)
                                                           gCAAGTCAGGCTGATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAAc                                                                                          <  2:354797/147‑1 (MQ=255)
                                                            cAAGTCAGGCTGATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGTTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAActgg                                                                                       <  8:363736/149‑1 (MQ=255)
                                                                   ggCTGATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGaa                                                                                <  7:203463/149‑1 (MQ=255)
                                                                    gCTGATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAAc                                                                               <  3:175804/149‑1 (MQ=255)
                                                                    gCTGATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAAc                                                                               >  8:56937/1‑149 (MQ=255)
                                                                       gATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACgg                                                                             <  2:324776/148‑1 (MQ=255)
                                                                       gATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACgg                                                                             >  1:324776/1‑148 (MQ=255)
                                                                       gATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGa                                                                            >  5:136707/1‑149 (MQ=255)
                                                                         ttATCAGGCCCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTCCTGTGGTCCCCGAGTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATg                                                                          <  5:348186/149‑1 (MQ=255)
                                                                         ttATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATg                                                                          >  6:3508/1‑149 (MQ=255)
                                                                              aGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTAc                                                                                                                                                 <  4:295242/73‑1 (MQ=255)
                                                                              aGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTAc                                                                                                                                                 >  3:295242/1‑73 (MQ=255)
                                                                               ggACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGTACGGATGCCAAcg                                                                    <  3:127562/149‑1 (MQ=255)
                                                                               ggACCCGTGGCTGAAACAACGGCTCGAAAATAACGGCTGGGTACTGTGGGCCCCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCTACCCTCCCGGCAAAACTGGCAGGGAACGGATGCCAAcg                                                                    >  6:260798/1‑149 (MQ=255)
                                                                                          tGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGTTACCAGTATCAACTTTGCTACCaa                                                                                                                            >  1:30443/1‑82 (MQ=255)
                                                                                          tGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGTTACCAGTATCAACTTTGCTACCaa                                                                                                                            <  2:30443/82‑1 (MQ=255)
                                                                                          tGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCGCCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGAtgtg                                                         >  2:42636/1‑149 (MQ=255)
                                                                                            aaaCAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCt                                                       >  1:48599/1‑149 (MQ=255)
                                                                                            aaaCAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCt                                                       <  1:111554/149‑1 (MQ=255)
                                                                                                   ggCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCCCg                                                 >  3:135291/1‑148 (MQ=255)
                                                                                                     cTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTTCCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCAt                                              >  7:130431/1‑149 (MQ=255)
                                                                                                     cTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCAt                                              >  1:199023/1‑149 (MQ=255)
                                                                                                     cTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCAt                                              <  2:6282/149‑1 (MQ=255)
                                                                                                       ggAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATtc                                            >  4:232384/1‑149 (MQ=255)
                                                                                                        gAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATtct                                           <  6:136707/149‑1 (MQ=255)
                                                                                                        gAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATtct                                           >  2:165331/1‑149 (MQ=255)
                                                                                                         aaaaTAACGGCTGGGTACTGTGGGCACCGATTCGCTTTCGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATACCAACGGCGGCGATGTGCTGGCACGCATtctc                                          >  7:291260/1‑149 (MQ=255)
                                                                                                           aaTAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTa                                        <  5:3508/149‑1 (MQ=255)
                                                                                                            aTAACGGCTTGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTAt                                       <  8:165142/149‑1 (MQ=255)
                                                                                                             tAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCATCGGCGGCGATGTGCTGGCACGCATTCTCTATg                                      >  1:261269/1‑149 (MQ=255)
                                                                                                             tAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATg                                      >  8:19835/1‑149 (MQ=255)
                                                                                                               aCGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTTCCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGc                                    <  8:130431/149‑1 (MQ=255)
                                                                                                               aCGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGc                                    <  2:199023/149‑1 (MQ=255)
                                                                                                                   cTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCAcgc                                <  7:276243/149‑1 (MQ=255)
                                                                                                                               ggCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGtt                     <  2:261269/148‑1 (MQ=255)
                                                                                                                               ggCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTc                    <  1:380668/149‑1 (MQ=255)
                                                                                                                                      aTTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAAcggcgg                                                                >  8:90936/1‑98 (MQ=255)
                                                                                                                                           cTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGa        <  2:48599/149‑1 (MQ=255)
                                                                                                                                               ggTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCt    >  5:93680/1‑149 (MQ=255)
                                                                                                                                                gTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATtc                                            >  4:341017/1‑108 (MQ=255)
                                                                                                                                                gTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATtc                                            <  3:341017/108‑1 (MQ=255)
                                                                                                                                                gTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCt    <  7:56937/148‑1 (MQ=255)
                                                                                                                                                gTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCt    <  5:162741/148‑1 (MQ=255)
                                                                                                                                                 tGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGa  <  7:19835/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCAGTTGGTATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGATTATCAGGACCCGTGGCTGAAACAACGGCTGGAAAATAACGGCTGGGTACTGTGGGCACCGATTCGCTTTGGTGCTACCAGTATCAACTTTGCTACCAATAAGCCCTTCCCTTCTCCACCCTCCCGGCAAAACTGGCTGGGAACGGATGCCAACGGCGGCGATGTGCTGGCACGCATTCTCTATGGCACGCGGATCTCGGTTCTGTTTGGCCTGATGCTGA  >  NC_000913/2275430‑2275723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: