Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 483,519 G→A 5.1% T295T (ACC→ACT acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913483,5190GA 5.1% 253.5 / 2.6 78T295T (ACC→ACTacrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (33/41);  new base A (2/2);  total (35/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.91e-01

CAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCC  >  NC_000913/483372‑483665
                                                                                                                                                   |                                                                                                                                                  
cAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGGCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCgg                                                                                                                                                   <  1:173831/149‑1 (MQ=255)
 aGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTAAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCgtt                                                                                                                                                  >  7:366184/1‑147 (MQ=255)
     ttttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGc                                                                                                                                               >  1:373/1‑148 (MQ=255)
     ttttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTAAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGc                                                                                                                                               >  4:217425/1‑148 (MQ=255)
            cACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGa                                                                                                                                       >  1:37660/1‑149 (MQ=255)
             aCTTCGTGAATAGTTATTTTCTCGAACGGCGTGGTGTCGTATGGGTTAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCTCGGATTGCCGGAGCGGTATCCAGCGGGTTTGCTCCGTTCGCCAGCTTGAt                                                                                                                                      <  6:374446/149‑1 (MQ=255)
             aCTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCAGt                                                                                                                                                  <  2:79687/137‑1 (MQ=255)
             aCTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCAGt                                                                                                                                                  <  2:74226/137‑1 (MQ=255)
             aCTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCAGt                                                                                                                                                  >  1:79687/1‑137 (MQ=255)
             aCTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCAGt                                                                                                                                                  >  1:74226/1‑137 (MQ=255)
                   tGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGCTccc                                                                                                                                   >  6:377903/1‑146 (MQ=255)
                    gAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGa                                                                                                                               >  1:1788/1‑149 (MQ=255)
                      aTAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGAc                                                                                                                              <  1:201547/148‑1 (MQ=255)
                         gagaTTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCgg                                                                                                                           >  6:253304/1‑148 (MQ=255)
                          agaTTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGaa                                                                                                                         <  7:176598/149‑1 (MQ=255)
                            aTTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCTGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTCGCTCACCAGACCGGACGc                                                                                                                       >  6:91563/1‑149 (MQ=255)
                               ttCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCgg                                                                                                                    >  3:344913/1‑149 (MQ=255)
                               ttCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCg                                                                                                                     <  4:17723/148‑1 (MQ=255)
                               ttCACGAACGGCGGGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCg                                                                                                                     <  5:284120/148‑1 (MQ=255)
                                  aCGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCgg                                                                                                                    >  6:25636/1‑146 (MQ=255)
                                  aCGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCgg                                                                                                                    <  5:25636/146‑1 (MQ=255)
                                   cGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGAGTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTgg                                                                                                                <  1:203390/149‑1 (MQ=255)
                                        ggCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATGTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGtt                                                                                                           <  5:193259/149‑1 (MQ=255)
                                        ggCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGCTCCCCAGACCGGAAGCCGGTTGGCCGt                                                                                                            >  2:2656/1‑148 (MQ=255)
                                        ggCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGtt                                                                                                           <  2:37660/149‑1 (MQ=255)
                                               ggtcggttGGGTAAACAATTTTCAGAACCGAAGGGAAGAACGGTTCCCTGTGCGCCAGGTCAGCTCGGATTGCCGCACCGGTATTCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAActct                                                                                                    <  3:186709/142‑1 (MQ=255)
                                                gtCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTCGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTg                                                                                                   <  6:75447/149‑1 (MQ=255)
                                                 tCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTg                                                                                                   <  3:131166/148‑1 (MQ=255)
                                                   gTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCga                                                                                                <  7:185081/149‑1 (MQ=255)
                                                   gTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCga                                                                                                >  6:95251/1‑149 (MQ=255)
                                                     aTGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCgat                                                                                               >  3:236359/1‑148 (MQ=255)
                                                        ggTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGatgatg                                                                                           >  5:134565/1‑149 (MQ=255)
                                                         gTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGt                                                                                          <  5:280531/149‑1 (MQ=255)
                                                             aCAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGc                                                                                                                                               <  5:72520/92‑1 (MQ=255)
                                                             aCAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGc                                                                                                                                               >  6:72520/1‑92 (MQ=255)
                                                             aCAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTa                                                                                      <  4:352275/149‑1 (MQ=255)
                                                             aCAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTa                                                                                      >  8:177302/1‑149 (MQ=255)
                                                              cAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAg                                                                                     <  7:177302/149‑1 (MQ=255)
                                                                   ttCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTtct                                                                                 <  4:344913/148‑1 (MQ=255)
                                                                      aGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTcac                                                                              <  6:292728/148‑1 (MQ=255)
                                                                          ccGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAActct                                                                                                    >  3:175140/1‑122 (MQ=255)
                                                                          ccGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAActct                                                                                                    <  4:175140/122‑1 (MQ=255)
                                                                          ccGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCaccacc                                                                          >  7:65118/1‑148 (MQ=255)
                                                                              cGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCt                                                                     >  5:380554/1‑149 (MQ=255)
                                                                              cGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCt                                                                     <  5:95251/149‑1 (MQ=255)
                                                                              cGGGAAGAACGGTTCCATCTTCGCCAGGTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGGCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCt                                                                     <  5:75991/149‑1 (MQ=255)
                                                                                   agaaCGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTa                                                                                      <  5:82253/127‑1 (MQ=255)
                                                                                   agaaCGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTa                                                                                      >  6:82253/1‑127 (MQ=255)
                                                                                    gaaCGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGtt                                                                                                                  >  5:265386/1‑98 (MQ=255)
                                                                                    gaaCGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGtt                                                                                                                  <  6:265386/98‑1 (MQ=255)
                                                                                     aaCGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCtt                                                              <  7:136911/149‑1 (MQ=255)
                                                                                      aCGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTc                                                             <  6:134565/149‑1 (MQ=255)
                                                                                              aTCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTc                                                     >  8:196092/1‑149 (MQ=255)
                                                                                                 ttCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACg                                                  >  3:278942/1‑149 (MQ=255)
                                                                                                    gCCAGTTCAGCACAGATTGCCTCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACgcag                                               <  1:181059/149‑1 (MQ=255)
                                                                                                      cAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACgcagca                                             <  5:253304/149‑1 (MQ=255)
                                                                                                            aGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCAcgc                                       >  4:224384/1‑149 (MQ=255)
                                                                                                               aCGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCgg                                     >  5:20769/1‑148 (MQ=255)
                                                                                                                       ccGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCCCCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATcc                            >  8:244995/1‑149 (MQ=255)
                                                                                                                          cAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGa                         <  5:129243/149‑1 (MQ=255)
                                                                                                                          cAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGa                         <  2:1788/149‑1 (MQ=255)
                                                                                                                                  tCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTGGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACttt                 <  4:236359/149‑1 (MQ=255)
                                                                                                                                    cAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGa                                   >  3:345408/1‑129 (MQ=255)
                                                                                                                                    cAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGa                                   <  4:345408/129‑1 (MQ=255)
                                                                                                                                    cAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTc                <  3:217425/148‑1 (MQ=255)
                                                                                                                                    cAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGATCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACttt                 >  8:95820/1‑147 (MQ=255)
                                                                                                                                    cAGCGCGTTTGCACCGGGCGCCAGCTTGATCCCCAGACCGGAATCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTca               <  8:366184/149‑1 (MQ=255)
                                                                                                                                     aGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTcag              >  4:250653/1‑149 (MQ=255)
                                                                                                                                      gcgcGTTTGCACCGGGCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTcagc             <  2:373/149‑1 (MQ=255)
                                                                                                                                       cgcgTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTcagca            <  6:380554/149‑1 (MQ=255)
                                                                                                                                        gcgTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGAGGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTcagcag           <  4:254394/149‑1 (MQ=255)
                                                                                                                                          gTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTa                                                                                      >  3:298199/1‑72 (MQ=255)
                                                                                                                                          gTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTa                                                                                      <  4:298199/72‑1 (MQ=255)
                                                                                                                                               cACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTg    <  3:250653/149‑1 (MQ=255)
                                                                                                                                               cACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTg    >  6:302383/1‑149 (MQ=255)
                                                                                                                                                aCCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTca               >  2:29871/1‑137 (MQ=255)
                                                                                                                                                aCCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTca               <  1:29871/137‑1 (MQ=255)
                                                                                                                                                 ccGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGcc  <  8:65118/149‑1 (MQ=255)
                                                                                                                                                  cGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGcc  >  4:180146/1‑148 (MQ=255)
                                                                                                                                                  cGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGcc  >  8:88048/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAGCGGTATCCAGCGCGTTTGCACCGGTCGCCAGCTTGATCCCCAGACCGGAAGCCGGTTGGCCGTTAAACTCTGCGATGATGTCGTAGTTCTCACCACCCAGCTCAATCTTCGCGACGTCACGCAGCAGCACGCGGGAACCATCCTGATTCACTTTCAGCAGGATTTTGCC  >  NC_000913/483372‑483665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: