Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,002,530 G→A G729D (GGT→GAT)  xdhA → xanthine dehydrogenase, molybdenum binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,002,5300GA98.1% 360.2 / ‑2.5 104G729D (GGT→GAT) xdhAxanthine dehydrogenase, molybdenum binding subunit
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (48/54);  total (49/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01

Only 100 of 109 total aligned reads displayed.

CCGACCATGCCGGATCTGCCACAACTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGGTGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCTTGCCGATGCCATCAACCTGCTGG  >  NC_000913/3002385‑3002674
                                                                                                                                                 |                                                                                                                                                
ccGACCATGCCGGATCTGCCACAACTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAATGCGGTGAAGATGGCTACCGatg                                                                                                                                                <  4:260877/148‑1 (MQ=255)
 cgcccATGCCGTATCTGCCACAACTGGACAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGtt                                                                                                                                              <  8:434368/146‑1 (MQ=255)
   acgaTGCCGGATCTGCCACAACTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGGCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGc                                                                                                                                            <  7:150918/146‑1 (MQ=255)
   aCCATGCTGGATCTGCCACAACTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTg                                                                                                                                             >  8:400194/1‑148 (MQ=255)
      aTGCCGGATCTGCCACAACTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTg                                                                                                                                             <  3:546559/145‑1 (MQ=255)
      aTGCCGGATCTGCCACAACTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTg                                                                                                                                             >  4:546559/1‑145 (MQ=255)
         ctggATCTGCCACAACTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGcaatcaa                                                                                                                                      <  7:400194/147‑1 (MQ=255)
         ccGGATCTGCCACAACTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGcaatcaa                                                                                                                                      <  2:268875/149‑1 (MQ=255)
         ccGGATCTGCCACAACTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGcaatcaa                                                                                                                                      <  1:546657/149‑1 (MQ=255)
         ccGGATCTGCCACAACTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGcaatca                                                                                                                                       <  7:96332/148‑1 (MQ=255)
          cGGATCTGCCACAACTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGcaatcaat                                                                                                                                     <  5:47409/149‑1 (MQ=255)
          cGGATCTGCCACAACTGGAAAGCGCGTGCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGcaatcaat                                                                                                                                     <  1:345698/149‑1 (MQ=255)
             aTCTGCCACAACTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATaca                                                                                                                                  >  7:449812/1‑149 (MQ=255)
              tCTGCCACAACTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTCGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGATGAAGATGGCTACCGATGTTGTAATCAATacac                                                                                                                                 >  2:318839/1‑149 (MQ=255)
                  ccACAACTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTg                                                                                                                               <  5:143960/147‑1 (MQ=255)
                   cacaACTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGca                                                                                                                                           >  3:515486/1‑134 (MQ=255)
                   cacaACTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGca                                                                                                                                           <  4:515486/134‑1 (MQ=255)
                   cacaACTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCg                                                                                                                            >  3:268786/1‑149 (MQ=255)
                    acaACTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGc                                                                                                                           >  2:396576/1‑149 (MQ=255)
                     caACTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGatgt                                                                                                                                               >  5:192843/1‑128 (MQ=255)
                     caACTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGatgt                                                                                                                                               <  6:192843/128‑1 (MQ=255)
                       aCTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGa                                                                                                                        <  2:261367/149‑1 (MQ=255)
                        cTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGAc                                                                                                                       <  5:2296/149‑1 (MQ=255)
                         tGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACg                                                                                                                      >  2:5156/1‑149 (MQ=255)
                           gAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGGTGTTGCAATCAATACACTGCCGCTGACGc                                                                                                                     >  1:168521/1‑148 (MQ=255)
                                    ttCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGatgt                                                                                                                                               <  3:492557/113‑1 (MQ=255)
                                    ttCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGt                                                                                                            >  2:52032/1‑148 (MQ=255)
                                        tcgAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAAc                                                                                                               <  3:23357/141‑1 (MQ=255)
                                        tcgAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAAc                                                                                                               >  4:23357/1‑141 (MQ=255)
                                         cgAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATg                                                                                                      <  1:5156/149‑1 (MQ=255)
                                           aaaTCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGGTGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGaa                                                                                                    <  2:168521/149‑1 (MQ=255)
                                           aaaTCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGaa                                                                                                    <  4:294981/149‑1 (MQ=255)
                                           aaaTCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGaa                                                                                                    <  8:449812/149‑1 (MQ=255)
                                             aTCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTg                                                                                                                                             <  8:275615/106‑1 (MQ=255)
                                              tCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGaa                                                                                                    >  5:308501/1‑146 (MQ=255)
                                              tCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGaa                                                                                                    <  6:308501/146‑1 (MQ=255)
                                                     gCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATc                                                                                          >  3:265419/1‑149 (MQ=255)
                                                         cAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGc                                                                                                                           >  7:265939/1‑112 (MQ=255)
                                                         cAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGc                                                                                                                           <  8:265939/112‑1 (MQ=255)
                                                          aaTCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTtat                                                                                                         >  7:612267/1‑129 (MQ=255)
                                                          aaTCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTtat                                                                                                         <  8:612267/129‑1 (MQ=255)
                                                          aaTCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTgg                                                                                       >  3:363090/1‑147 (MQ=255)
                                                           aTCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAg                                                                                    >  2:295567/1‑149 (MQ=255)
                                                                cATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTta                                                                                                          <  2:42693/122‑1 (MQ=255)
                                                                cATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTta                                                                                                          >  1:42693/1‑122 (MQ=255)
                                                                    cGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGAttt                                                                           <  1:295567/149‑1 (MQ=255)
                                                                     ggACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGAttt                                                                           >  7:274851/1‑148 (MQ=255)
                                                                      gACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGa                                                                         <  4:268786/149‑1 (MQ=255)
                                                                      gACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGAGGTGAAGATGGCTACAGATGTTGTAATCAATACACTGCCGCTGAATCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGa                                                                         <  1:318839/149‑1 (MQ=255)
                                                                       aCATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGAc                                                                                                                       <  7:339790/102‑1 (MQ=255)
                                                                       aCATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGAc                                                                                                                       >  8:339790/1‑102 (MQ=255)
                                                                       aCATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAg                                                                        <  7:173710/149‑1 (MQ=255)
                                                                       aCATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAg                                                                        >  8:173710/1‑149 (MQ=255)
                                                                        cATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAg                                                                        >  3:158207/1‑148 (MQ=255)
                                                                         aTAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCaaaa                                                                                                                >  8:266521/1‑107 (MQ=255)
                                                                         aTAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCaaaa                                                                                                                <  7:266521/107‑1 (MQ=255)
                                                                           aaGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGAt                                                                     >  4:161653/1‑148 (MQ=255)
                                                                              tCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAAc                                                                                                               >  8:275722/1‑103 (MQ=255)
                                                                              tCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAAc                                                                                                               <  7:275722/103‑1 (MQ=255)
                                                                                aCTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAAcatc                                                               <  3:445006/149‑1 (MQ=255)
                                                                                aCTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAAcat                                                                >  2:294470/1‑148 (MQ=255)
                                                                                 cTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAAcatca                                                              >  3:103293/1‑149 (MQ=255)
                                                                                 cTGGGTGAGCCACCAATAATTCCTCTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTAATATGAAGAGTTCCTACTGGCAGGATTGATTTGAGGATAAcatca                                                              >  2:115012/1‑149 (MQ=255)
                                                                                     gTGAGCCACCAATAATTCCTCTTGCCGCTTCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGa                                                                                                                        <  4:261521/87‑1 (MQ=255)
                                                                                        aGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTg                                                        <  4:265419/148‑1 (MQ=255)
                                                                                          ccaccaATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGgatt                                                                                >  7:521519/1‑122 (MQ=255)
                                                                                          ccaccaATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGgatt                                                                                <  8:521519/122‑1 (MQ=255)
                                                                                          ccaccaATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAAc                                                                  <  1:203085/136‑1 (MQ=255)
                                                                                          ccaccaATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAAc                                                                  >  2:203085/1‑136 (MQ=255)
                                                                                           caccaATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGgattga                                                                              <  4:66526/123‑1 (MQ=255)
                                                                                            accaATAATTCCTGTTGCGGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGAtttt                                                   >  7:281010/1‑149 (MQ=255)
                                                                                            accaATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACATTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGAtttt                                                   >  5:40398/1‑149 (MQ=255)
                                                                                            accaATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAg                                                                        <  8:504149/128‑1 (MQ=255)
                                                                                            accaATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAg                                                                        >  7:504149/1‑128 (MQ=255)
                                                                                             ccaATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCaaaa                                                                                                                >  3:251926/1‑87 (MQ=255)
                                                                                             ccaATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCaaaa                                                                                                                <  4:251926/87‑1 (MQ=255)
                                                                                                   atTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGcttct                                             <  1:396576/148‑1 (MQ=255)
                                                                                                    ttCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGcaat                                                                                                                                         <  2:510708/55‑1 (MQ=255)
                                                                                                    ttCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGgattgatt                                                                            <  4:27760/116‑1 (MQ=255)
                                                                                                    ttCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGgattgatt                                                                            >  3:27760/1‑116 (MQ=255)
                                                                                                              cGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACgg                                                                                                             <  1:392169/73‑1 (MQ=255)
                                                                                                              cGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCgca                                 <  1:294470/149‑1 (MQ=255)
                                                                                                               gctgctATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCa                                                                                     <  4:509126/96‑1 (MQ=255)
                                                                                                               gctgctATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCa                                                                                     >  3:509126/1‑96 (MQ=255)
                                                                                                                         gTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCTTg                       <  4:103293/148‑1 (MQ=255)
                                                                                                                             cgcgGTGAAGATGGCTTCCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGTACTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCTTGCCGAt                  <  1:115012/149‑1 (MQ=255)
                                                                                                                              gcgGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTAc                                          <  1:599016/124‑1 (MQ=255)
                                                                                                                              gcgGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTAc                                          >  2:599016/1‑124 (MQ=255)
                                                                                                                                  tGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCgca                                 <  3:389136/129‑1 (MQ=255)
                                                                                                                                  tGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCTTGCCGATGCCAt             >  6:410188/1‑149 (MQ=255)
                                                                                                                                  tGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCAGGTTTGATTTTGCTTCTTACCATCGCgca                                 >  4:389136/1‑129 (MQ=255)
                                                                                                                                   gAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCTTGCCGATGCCAt             >  1:368852/1‑148 (MQ=255)
                                                                                                                                      gATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGga                                                                                  >  1:582332/1‑76 (MQ=255)
                                                                                                                                      gATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGga                                                                                  <  2:582332/76‑1 (MQ=255)
                                                                                                                                          gCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCTTGCCGATGCCATCAACctgc     >  3:379808/1‑149 (MQ=255)
                                                                                                                                            tACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCTTGCCGATg                 <  6:323739/135‑1 (MQ=255)
                                                                                                                                            tACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCTTGCCGATg                 >  5:323739/1‑135 (MQ=255)
                                                                                                                                              ccGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCgcag                                >  8:246793/1‑118 (MQ=255)
                                                                                                                                              ccGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCgcag                                <  7:246793/118‑1 (MQ=255)
                                                                                                                                              ccGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCTTGCCGATGCCATCAACCTgctgg  <  5:410188/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CCGACCATGCCGGATCTGCCACAACTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGGTGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCTTGCCGATGCCATCAACCTGCTGG  >  NC_000913/3002385‑3002674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: