Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,223,210 G→A 5.2% W193* (TGG→TAG)  ebgA → evolved beta‑D‑galactosidase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,223,2100GA 5.2% 232.5 / 2.2 76W193* (TGG→TAG) ebgAevolved beta‑D‑galactosidase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (31/41);  new base A (2/2);  total (33/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.25e-01

AACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCC  >  NC_000913/3223062‑3223355
                                                                                                                                                    |                                                                                                                                                 
aaCGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATgtggtg                                                                                                                                                   >  6:350746/1‑149 (MQ=255)
   ggTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGCCCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTggtggt                                                                                                                                                 >  3:5437/1‑148 (MQ=255)
    gTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTggtggt                                                                                                                                                 >  4:142782/1‑147 (MQ=255)
     tCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGGTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAg                                                                                                                                              <  1:272276/149‑1 (MQ=255)
         tATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCggg                                                                                                                                          <  2:300119/149‑1 (MQ=255)
           tgtgGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATTGTTAAAACCGTCGAGAACCTGTTGTGTGTGCGCGTTATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGa                                                                                                                                        <  5:103709/149‑1 (MQ=255)
            gtgGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGAt                                                                                                                                       >  3:123871/1‑149 (MQ=255)
             tgGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGAt                                                                                                                                       <  3:142782/148‑1 (MQ=255)
              gggTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGc                                                                                                                                             >  4:266680/1‑141 (MQ=255)
              gggTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGc                                                                                                                                             <  3:266680/141‑1 (MQ=255)
                     aGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCg                                                                                                                                            <  8:382234/135‑1 (MQ=255)
                     aGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCg                                                                                                                                            >  7:382234/1‑135 (MQ=255)
                      gCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGAt                                                                                                                             <  6:119826/149‑1 (MQ=255)
                       cAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGAGAACCTGTTTTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGAt                                                                                                                             <  5:328137/148‑1 (MQ=255)
                       cAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGTACTCTACCAACGTGGAAGCCCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGag                                                                                                                             >  6:328137/1‑147 (MQ=255)
                          gggCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAACCCGGCGACACCCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGtt                                                                                                                          >  1:300838/1‑148 (MQ=255)
                             cAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTTCCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATc                                                                                                                      <  2:32443/149‑1 (MQ=255)
                              aGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGTGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCt                                                                                                                     <  5:39783/149‑1 (MQ=255)
                              aGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCt                                                                                                                     >  8:389570/1‑149 (MQ=255)
                                  gCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGt                                                                                                                  <  3:44131/148‑1 (MQ=255)
                                  gCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCATGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGGCAGCGGGGAGCTTCCGCGATGTTTATCTGGt                                                                                                                  <  2:52127/148‑1 (MQ=255)
                                   ccTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTAGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCg                                                                                                                >  7:277747/1‑149 (MQ=255)
                                   ccTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTAGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCg                                                                                                                >  7:283267/1‑149 (MQ=255)
                                        ccGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGaaaa                                                                                                           <  1:213259/149‑1 (MQ=255)
                                         cGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGaaaa                                                                                                           >  6:136122/1‑148 (MQ=255)
                                           cAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGCTTATCTGGTCGGAAAacac                                                                                                        >  2:77321/1‑149 (MQ=255)
                                            agagTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACAcc                                                                                                       <  4:159776/149‑1 (MQ=255)
                                                gttGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGGCGGAAAACACCTAAc                                                                                                   <  3:219032/148‑1 (MQ=255)
                                                    aCATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCat                                                                                               <  7:389570/149‑1 (MQ=255)
                                                    aCATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCa                                                                                                <  4:5437/148‑1 (MQ=255)
                                                         aGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCTATGTTTATCTGGTCGGAAAACACCTAACGCATATTaa                                                                                          <  4:123871/149‑1 (MQ=255)
                                                              gATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTAGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGAtt                                                                                     <  8:283267/149‑1 (MQ=255)
                                                              gATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTAGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACCCATATTAACGAtt                                                                                     <  8:277747/149‑1 (MQ=255)
                                                                      aaaCCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACtgtg                                                                             <  5:350746/149‑1 (MQ=255)
                                                                        aCCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCg                                                                           >  2:200185/1‑149 (MQ=255)
                                                                                cAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACt                                                                   >  4:48370/1‑149 (MQ=255)
                                                                                  aCCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACttt                                                                 <  3:69213/149‑1 (MQ=255)
                                                                                    cTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCt                                                                                                      <  2:236988/110‑1 (MQ=255)
                                                                                     tgttgtGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACtgt                                                                              >  1:38733/1‑133 (MQ=255)
                                                                                     tgttgtGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACtgt                                                                              <  2:38733/133‑1 (MQ=255)
                                                                                     tgttgtGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACATTGAc                                                              >  8:310049/1‑149 (MQ=255)
                                                                                      gttgtGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACGAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCCCtgtg                                                                             <  1:322335/133‑1 (MQ=255)
                                                                                      gttgtGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACAAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACtgtg                                                                             >  2:322335/1‑133 (MQ=255)
                                                                                        tgtgtgtgCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAAc                                                                                         >  1:247964/1‑119 (MQ=255)
                                                                                        tgtgtgtgCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAAc                                                                                         <  2:247964/119‑1 (MQ=255)
                                                                                          tgtgtgCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGc                                                         <  3:255927/149‑1 (MQ=255)
                                                                                            tgtgCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGATTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCTACTTTGACGAAGcc                                                        >  7:163252/1‑148 (MQ=255)
                                                                                                  cgTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTa                                                                         <  4:151539/125‑1 (MQ=255)
                                                                                                  cgTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTa                                                                         >  3:151539/1‑125 (MQ=255)
                                                                                                  cgTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACGTTGCCGAAGCCTATTGCg                                                 >  6:287423/1‑149 (MQ=255)
                                                                                                   gTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCg                                                                                                                                            >  7:192779/1‑57 (MQ=255)
                                                                                                   gTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCg                                                                                                                                            <  8:192779/57‑1 (MQ=255)
                                                                                                   gTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCGTATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGa                                                >  8:30484/1‑149 (MQ=255)
                                                                                                   gTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGa                                                <  7:170503/149‑1 (MQ=255)
                                                                                                    tGATGCAGTGGGCGGACTCTACCTAGGTGGAAGACCAGGTTATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGAt                                                                                      <  1:218336/110‑1 (MQ=255)
                                                                                                       tGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGTGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGcc                                            <  1:200185/149‑1 (MQ=255)
                                                                                                        gCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCa                                           >  2:233120/1‑149 (MQ=255)
                                                                                                         cAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCa                                           <  6:138433/148‑1 (MQ=255)
                                                                                                            tGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCt                                       >  6:170871/1‑149 (MQ=255)
                                                                                                               gCGGACTCTACCTACGTGGAAGACGAGGATATGTGGTGGGCAGCGGGGATCTTCCGCGATGTTTTTCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTc                                    <  3:324793/149‑1 (MQ=255)
                                                                                                                cGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGGCAGCGGGGATCTTCCGCGATGTTTATCTGGCCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTcc                                   <  6:177109/149‑1 (MQ=255)
                                                                                                                 ggACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCt                                  <  5:136122/149‑1 (MQ=255)
                                                                                                                 ggACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGCTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCt                                  <  1:77321/149‑1 (MQ=255)
                                                                                                                  gACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTg                                 >  8:239519/1‑149 (MQ=255)
                                                                                                                       tacctacGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGAt                                               <  6:226898/130‑1 (MQ=255)
                                                                                                                       tacctacGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGAt                                               >  5:226898/1‑130 (MQ=255)
                                                                                                                       tacctacGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAg                            >  1:166942/1‑149 (MQ=255)
                                                                                                                         cctacGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGaa                             <  3:48370/146‑1 (MQ=255)
                                                                                                                         cctacGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAgtg                          >  4:107153/1‑149 (MQ=255)
                                                                                                                         cctacGAGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAgt                           <  8:394352/148‑1 (MQ=255)
                                                                                                                          ctacGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAgtgg                         >  3:156509/1‑149 (MQ=255)
                                                                                                                          ctacGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAgtgg                         >  7:146886/1‑149 (MQ=255)
                                                                                                                                     aCCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGCTGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGaaaa               >  4:123793/1‑148 (MQ=255)
                                                                                                                                     aCCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAAt              >  2:165594/1‑149 (MQ=255)
                                                                                                                                       cAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGATAACACCTAACGCATATTAACGATTTCACTGTGAGTACCGACTTTGACGAAGCCTCTTGCGATGCCACGCGTTCCTGCGAAGTGGTGCTGGAAAAtct            >  8:136781/1‑149 (MQ=255)
                                                                                                                                        aGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAACCACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAAtctc           >  2:187533/1‑149 (MQ=255)
                                                                                                                                        aGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCGTATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAAtctc           <  7:30484/149‑1 (MQ=255)
                                                                                                                                           atatGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTcgcc        <  2:300838/149‑1 (MQ=255)
                                                                                                                                           atatGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACATTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGGGGTGCTGGAAAATCTcgcc        <  7:310049/149‑1 (MQ=255)
                                                                                                                                                 ggtggtCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTcc  >  6:69798/1‑149 (MQ=255)
                                                                                                                                                 ggtggtCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTc   <  4:61521/148‑1 (MQ=255)
                                                                                                                                                 ggtggtCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGAGAAGTGGTGCTGGAAAATCTCGCCGCCTc   >  3:61521/1‑148 (MQ=255)
                                                                                                                                                   tggtCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGAt                                               >  6:364486/1‑102 (MQ=255)
                                                                                                                                                   tggtCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGAt                                               <  5:364486/102‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
AACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTTGACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCCTCC  >  NC_000913/3223062‑3223355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: