Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,614,626 C→A 5.5% intergenic (+366/+54) yjjV → / ← yjjW putative DNase/putative pyruvate formate lyase activating enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,614,6260CA 5.5% 279.5 / 2.6 82intergenic (+366/+54)yjjV/yjjWputative DNase/putative pyruvate formate lyase activating enzyme
Reads supporting (aligned to +/- strand):  ref base C (39/38);  new base A (3/2);  total (42/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTCCGGCGTGGCGCTCGCCC  >  NC_000913/4614479‑4614774
                                                                                                                                                   |                                                                                                                                                    
aCGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGAcg                                                                                                                                                     >  1:71250/1‑149 (MQ=255)
 cgccgcATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTATGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCCGCAGTTGGCGCACAATGCCTGATGCGACGCTTGAcgc                                                                                                                                                    >  1:173443/1‑149 (MQ=255)
   ccgcATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGt                                                                                                                                                  >  6:47092/1‑149 (MQ=255)
    cgcATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTc                                                                                                                                                 <  4:142352/149‑1 (MQ=255)
     gcATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCt                                                                                                                                                >  6:12413/1‑149 (MQ=255)
          cGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATc                                                                                                                                            <  3:276296/148‑1 (MQ=255)
           ggCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAg                                                                                                                                          >  8:40943/1‑149 (MQ=255)
           ggCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAg                                                                                                                                          <  2:71250/149‑1 (MQ=255)
             cAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGc                                                                                                                                        <  8:75442/149‑1 (MQ=255)
                    cgcATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAg                                                                                                                                 >  3:312332/1‑149 (MQ=255)
                    cgcATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGAGTCTTATCAGGCCTACAAg                                                                                                                                 >  6:5399/1‑149 (MQ=255)
                             ccTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGTATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCg                                                                                                                        <  4:123275/149‑1 (MQ=255)
                                 aTGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAAcc                                                                                                                    >  6:141269/1‑149 (MQ=255)
                                    cGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCTGGCCTACAAGTCTGTGCCGAACCGTa                                                                                                                 <  7:21153/149‑1 (MQ=255)
                                     gACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACaa                                                                                                                                  >  7:31767/1‑131 (MQ=255)
                                     gACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACaa                                                                                                                                  <  8:25975/131‑1 (MQ=255)
                                     gACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACaa                                                                                                                                  <  8:31767/131‑1 (MQ=255)
                                     gACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACaa                                                                                                                                  >  7:25975/1‑131 (MQ=255)
                                     gACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAg                                                                                                                >  7:344490/1‑149 (MQ=255)
                                     gACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCAAACGGCAGGTGGCGCACAATGCCTGATGCGACGCTTGAAGCTTCTTATCAGGCCTACAAGTCTTTGCCGAACCGTAg                                                                                                                >  2:226025/1‑149 (MQ=14)
                                      aCGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAgg                                                                                                               >  2:47833/1‑149 (MQ=255)
                                             tCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGTCGTATc                                                                                                        >  4:305355/1‑149 (MQ=255)
                                             tCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATc                                                                                                        >  3:292092/1‑149 (MQ=40)
                                               gcctCTTATCATGCCTACAGGTTTGTTCCGAACCGTAGGCCGGATAAGGCGTTCGCGCAGCATCCGGCACAAGGCGCACAAAGCATAATGCGACGCTTGACGCGACTTATAAGGCCTACAAGTCTGTGCCGAACCGTACGCCTTaacag                                                                                                      >  4:352785/4‑145 (MQ=11)
                                                 gTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGc                                                                                                    <  1:241110/149‑1 (MQ=255)
                                                     tATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGt                                                                                                >  6:4108/1‑149 (MQ=255)
                                                      aTCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATg                                                                                                 <  7:44598/147‑1 (MQ=255)
                                                      aTCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCGGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGt                                                                                                <  1:226025/148‑1 (MQ=255)
                                                       tCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTc                                                                                               <  4:312332/148‑1 (MQ=255)
                                                         aTGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTcac                                                                                             <  2:130278/148‑1 (MQ=255)
                                                             cTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATa                                                                                        <  1:333984/149‑1 (MQ=255)
                                                                cAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGGATGTCACAAATaga                                                                                      <  1:47833/148‑1 (MQ=255)
                                                                cAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATag                                                                                       <  5:4108/147‑1 (MQ=255)
                                                                            gAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTa                                                                                                                 <  5:325702/109‑1 (MQ=40)
                                                                            gAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTa                                                                                                                 >  6:325702/1‑109 (MQ=40)
                                                                                 gTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAAcc                                                                    <  7:40943/149‑1 (MQ=255)
                                                                                   agtccGTATAAGGCGTTCGCGCCGCATCCTGCAGTTGGCGCACAATGCATGATGCGACGGTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCg                                                                   <  3:352785/145‑1 (MQ=21)
                                                                                      ccGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTc                                                               <  4:292092/149‑1 (MQ=255)
                                                                                            aaGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCcg                                                         >  2:306729/1‑149 (MQ=255)
                                                                                                 gTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTTCCGAACCGTAGGCCGTATCCGGCa                                                                                                   <  8:237934/102‑1 (MQ=255)
                                                                                                 gTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTTCCGAACCGTAGGCCGTATCCGGCa                                                                                                   >  7:237934/1‑102 (MQ=255)
                                                                                                  ttCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCAcc                                                   >  3:305199/1‑149 (MQ=255)
                                                                                                     gcgcCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCtt                                                 >  1:341979/1‑148 (MQ=255)
                                                                                                      cgccgcATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCttt                                                >  4:353363/1‑148 (MQ=255)
                                                                                                       gccgcATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTaa                                              <  1:306729/149‑1 (MQ=255)
                                                                                                       gccgcATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTaa                                              >  5:177049/1‑149 (MQ=255)
                                                                                                        ccgcATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAAc                                             <  5:5399/149‑1 (MQ=255)
                                                                                                            aTCCGGCTGTTGCCGCACCATGCCTGATGCGACGCTGGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCAt                                         <  2:293760/149‑1 (MQ=255)
                                                                                                               cGGCAGTTGGCGGACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAg                                      <  5:128848/149‑1 (MQ=255)
                                                                                                               cGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAAt                                                                                         <  5:367295/98‑1 (MQ=255)
                                                                                                               cGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAAt                                                                                         >  6:367295/1‑98 (MQ=255)
                                                                                                               cGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCAt                                         >  6:30095/1‑146 (MQ=255)
                                                                                                               cGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCa                                       <  5:30095/148‑1 (MQ=255)
                                                                                                                   aGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCaa                                  <  4:305199/149‑1 (MQ=255)
                                                                                                                     ttGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACg                                >  4:149383/1‑149 (MQ=255)
                                                                                                                        gcgcACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGc                              <  8:344490/148‑1 (MQ=255)
                                                                                                                        gcgcACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCt                             >  3:351826/1‑149 (MQ=255)
                                                                                                                        gcgcACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCt                             >  3:103524/1‑149 (MQ=255)
                                                                                                                          gcACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCa                                       <  5:73856/137‑1 (MQ=255)
                                                                                                                          gcACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCa                                       >  6:73856/1‑137 (MQ=255)
                                                                                                                           cacaATGCCTTATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCaa                          <  6:93294/149‑1 (MQ=255)
                                                                                                                           cacaATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGc                                           >  3:270152/1‑132 (MQ=255)
                                                                                                                           cacaATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGc                                           <  4:270152/132‑1 (MQ=255)
                                                                                                                            acaATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAAc                         >  6:11123/1‑149 (MQ=255)
                                                                                                                            acaATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAAc                         <  5:141269/149‑1 (MQ=255)
                                                                                                                               aTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTc                      <  5:52673/149‑1 (MQ=255)
                                                                                                                                   cTGATAAGACGCGTCAAGCGTCGCATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTcc                  >  7:197869/1‑149 (MQ=255)
                                                                                                                                      ataagACGCGTCAAGCGTCGCATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGc                                                                 <  3:54111/95‑1 (MQ=37)
                                                                                                                                      ataagACGCGTCAAGCGTCGCATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGc                                                                 >  4:54111/5‑99 (MQ=37)
                                                                                                                                      ataagACGCGTCAAGCGTCGCATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTCCGGc               <  8:197869/145‑1 (MQ=255)
                                                                                                                                      aTGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTc                            <  3:14527/136‑1 (MQ=255)
                                                                                                                                      aTGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTc                            >  4:14527/1‑136 (MQ=255)
                                                                                                                                      aTGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTCCGGc               >  1:345607/1‑149 (MQ=255)
                                                                                                                                         cGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTCCGGCGt             <  5:12413/148‑1 (MQ=255)
                                                                                                                                          gACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAata                                                                          >  6:10767/1‑86 (MQ=255)
                                                                                                                                          gACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAata                                                                          <  5:10767/86‑1 (MQ=255)
                                                                                                                                          gACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTCCg                 >  1:240490/1‑143 (MQ=255)
                                                                                                                                          gACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTCCg                 <  2:240490/143‑1 (MQ=255)
                                                                                                                                           aCGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTCCGGCGTGgc          >  6:274935/1‑149 (MQ=255)
                                                                                                                                           aCGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTCCGGCGTGgc          >  5:23344/1‑149 (MQ=255)
                                                                                                                                            cGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTCCGGCGTGgcg         >  4:138533/1‑149 (MQ=255)
                                                                                                                                               ttGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCACGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTCCGGCGTGGCGCTc      >  8:192637/1‑149 (MQ=255)
                                                                                                                                               ttGACGCGTCTTATCAGGCCTACAAGACTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCCACCGGCTCACCCCGCGCCCCTGTAACGCATAAGCCAACGGCTCAACGTCTTCCGGCGTGGCGCTc      >  4:263594/1‑149 (MQ=39)
                                                                                                                                                  aCGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTCCGGCGTGGCGCTCGcc   >  5:53264/1‑149 (MQ=255)
                                                                                                                                                   cgcgTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTCCGGCGTGGCGCACGccc  >  2:388420/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAAGTCTGTGCCGAACCGTAGGCCGTATCCGGCATGTCACAAATAGAGCGCCGGAAATATCAACCGGCTCACCCCGCGCACCTTTAACGCATCAGCCAACGGCTCAACGTCTTCCGGCGTGGCGCTCGCCC  >  NC_000913/4614479‑4614774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: