Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,152,558 G→A 6.1% R207H (CGT→CAT)  fabF → 3‑oxoacyl‑[acyl‑carrier‑protein] synthase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,152,5580GA 6.1% 204.1 / 2.8 65R207H (CGT→CAT) fabF3‑oxoacyl‑[acyl‑carrier‑protein] synthase II
Reads supporting (aligned to +/- strand):  ref base G (27/34);  new base A (2/2);  total (29/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.97e-01

TCGCGACTGCCTGTACTTCCGGCGTGCACAACATTGGCCATGCTGCGCGTATTATCGCGTATGGCGATGCTGACGTGATGGTTGCAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTGGGATAAAGAGCGTGATGGTTTCGTACTGGGCGATGGTGCCGGTATGCTGGTACTTGAAGAGTACGAACACGCGAAAAAACGCGGTGCGAAAATTTACGCT  >  NC_000913/1152417‑1152706
                                                                                                                                             |                                                                                                                                                    
tCGCGACTGCCTGTACTTCCGGCGTGCACAACATTGGCCATGCTGCGCGTATTATCGCGTATGGCGATGCTGACGTGATGGTTGCAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCAt                                                                                                                                                 <  5:99605/147‑1 (MQ=255)
tCGCGACTGCCTGTACTTCCGGCGTGCACAACATTGGCCATGCTGCGCGTATTATCGCGTATGGCGATGCTGACGTGATGGTTGCAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCAt                                                                                                                                                 <  2:318113/147‑1 (MQ=255)
     aCTGCCTGTACTTCCGGCGTGCACAACATTGGCCATGCTGCGCGTATTATCGCGTATGGCGATGCTGACGTGATGGTTGCAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTAc                                                                                                                                          <  6:18972/149‑1 (MQ=255)
        tccTGTACTTCCGGCGTGCACAACATTGGCAATGCTGAGAGAATTATCTCTTATGGAGATGATGACGTTAGGGTTGCATTTGGCTCAGAGAAAGCCAGTACGCCTCTGGGAGTTGGTGGTTTTGGAGCGGCAAGTGCATTATATAACCg                                                                                                                                       >  7:270529/2‑149 (MQ=255)
        gCCTGTAATTCCGGCGTGCACAACATTGGCCATGCTGCGCGTATTATCTAGTATGGCGATGCTGACGTGATGGTTGCAGTTGGAGCAGAGAAAGCCAGTAAGACTGTGGGAGTTTGTTGTTTTGGAGAGGCTCGAGCATTATATAACCg                                                                                                                                       >  7:270527/1‑149 (MQ=255)
         ccTGTACTTCCGGCGTGCACAACATTGGCCATGCTGCGCGTATTATTGCGTATGGCGATGCTGACGTGATGGTTGCAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCg                                                                                                                                       >  7:198214/1‑148 (MQ=255)
          cTGTACTTCCGGCGTGCACAACATTGGCCATGCTGCGCGTATTATCGCGTATGGCGATGCTGACGTGATGGTTGCAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGc                                                                                                                                      >  4:281361/1‑148 (MQ=255)
             tACTTCCGGCGTGCACAACATTGGCCATGCTGCGCGTATTATCGCGTATGGCGATGCTGACGTGATGGTTGCAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCaa                                                                                                                                    <  3:139390/147‑1 (MQ=255)
               cTTCCGGCGTGCACAACATTCGCCATGCTGCGCGTATTATCGCGTATGGCGATGCTGACGTGATGGTGGCAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGAt                                                                                                                                <  2:69536/149‑1 (MQ=255)
                        tGCACAACATTGGCCATGCTGCGCGTATTATCGCGTATGGCGATGCTGACGTGATGGTTGCAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCaa                                                                                                                       <  7:187952/149‑1 (MQ=255)
                            caacaTTGGCCATGCTGCGCGTATTATCGCGTATGGCGATGCTGACGTGATGGTTGCAGGTGGCGTAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCATCAAgcgg                                                                                                                   >  3:107958/1‑149 (MQ=255)
                             aataTTGGCCATTATGCGCGGATTATCGCGTATGGCTATTATTACGTGATGGGTGCAGTGGGAGCAGAGAAATCCAGTACGACGCTTGGCGTTGGTGGTTTTTTCGCGTAACGGGCATTATCTACCCGCAATGATAACCCGCAAgcggc                                                                                                                  <  8:270527/146‑1 (MQ=255)
                             aacaGTTGCCATGATGCGCGTATTATCGCGTATGGCGATGCGGACGTGATGGGTGCATGGGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAgcggc                                                                                                                  <  8:270529/149‑1 (MQ=255)
                                 ttGGCCATGCTGCGCGTATTATCGCGTATGGCGATGCTGACGTGATGGTTGCAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAgc                                                                                                              >  3:243337/1‑149 (MQ=255)
                                    gCCATGCTGCGCGTATTATCGCGTATGGCGATGCTGACGTGATGGTTGCAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAgccgc                                                                                                           >  5:221383/1‑149 (MQ=255)
                                     ccATGCTGCGCGTATTATCGCGTATGGCGATGCTGACGTGATGGTTGCAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAgccgcc                                                                                                          >  7:284440/1‑149 (MQ=255)
                                      cATGCTGCGCGTATTATCGCGTATGGCGATGCTGACGTGATGGTTGCAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGccc                                                                                                         >  1:4321/1‑149 (MQ=255)
                                      cATGCTGCGCGTATTATCGCGTATGGCGATGCTGACGTGATGGTTGCAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGccc                                                                                                         >  5:111748/1‑149 (MQ=255)
                                         gctgcGCGTATTATCGCGTATGGCGATGCTGACGTGATGGTTGCAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCAtt                                                                                                                                                <  7:136673/107‑1 (MQ=255)
                                         gctgcGCGTATTATCGCGTATGGCGATGCTGACGTGATGGTTGCAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCAtt                                                                                                                                                >  8:136673/1‑107 (MQ=255)
                                             cgcgTATTATTGCGTATGGCGATGCTGACGTGATGGTTGCAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTggg                                                                                                    <  8:198214/147‑1 (MQ=255)
                                              gcgTATTATCGCGTATGGCGATGCTGACGTGATGGTTGCAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAgcggc                                                                                                                  <  6:230003/132‑1 (MQ=255)
                                              gcgTATTATCGCGTATGGCGATGCTGACGTGATGGTTGCAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAgcggc                                                                                                                  >  5:230003/1‑132 (MQ=255)
                                               cgTATTATCGCGTATGGCGATGCTGACGTGATGGTTGCAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTGGGATaa                                                                                                <  2:53636/149‑1 (MQ=255)
                                                 tattatCGCGTATGGCGATGCTGACGTGATGGTTGCAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTGGGATAAag                                                                                              <  6:278541/149‑1 (MQ=255)
                                                   ttatCGCGTATGGCGATGCTGACGTGATGGTTGCAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTGGGATAAagag                                                                                            <  6:244111/149‑1 (MQ=255)
                                                   ttatCGCGTATGGCGATGCTGACGGTATGGTTGCAGGTGGCGCTGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTGGGATAAaga                                                                                             <  6:124076/148‑1 (MQ=255)
                                                      tCGCGTATGGCGATGCTGACGTGATGGTTGCAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTGGGATAAAGAGCGt                                                                                         >  7:113862/1‑149 (MQ=255)
                                                      tCGCGTATGGCGATGCTGACGTGATGGTTGCAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACATGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTGGGATAAAGAGCGt                                                                                         >  1:137864/1‑149 (MQ=255)
                                                      tCGCGTATGGCGATGCTGACGTGATGGTTGCAGGTGGCGCAGAGAAAGCCAGAACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACATGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTGGGATAAAGAGCGt                                                                                         >  1:132415/1‑149 (MQ=255)
                                                         cgTATGGCGATGCTGACGTGATGGTTGCAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTGGGATAAAGAGCGTGAt                                                                                      <  3:24785/149‑1 (MQ=255)
                                                             tGGCGATGCTGACGTGATGGTTGCAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTGGGATAAAGAGCGTGATGGtt                                                                                  >  7:384796/1‑149 (MQ=255)
                                                               gcgcTGCTGACGTGATGGTTGCAGGTGGCGCAGAGAAAGCCAGTACGCCGCGTGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTCTCTACCCGCAATGATAACCCGCAAGCGGCGCGACGCCCGAGGGATAAATAGCGTGATGGTTTc                                                                                >  4:267901/1‑149 (MQ=255)
                                                               gCGATGCTGACGTGATGGTTGCAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTGGGATAAAGAGCGTGATGGTTTc                                                                                <  2:156898/149‑1 (MQ=255)
                                                                 gATGCTGACGTGATGGTTGCAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTGGGATAAAGAGCGTGATg                                                                                     >  1:218064/1‑142 (MQ=255)
                                                                 gATGCTGACGTGATGGTTGCAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTGGGATAAAGAGCGTGATg                                                                                     <  2:218064/142‑1 (MQ=255)
                                                                  aTGCTGACGTGATGGTTGCAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTGGGATAAAGAGCGTGATGGTTTCGt                                                                              >  1:198560/1‑148 (MQ=255)
                                                                          gTGATGGTTGCAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTGGGATAAAGAGCGTGATGGTTTCGTACTGGGCGa                                                                     >  3:278998/1‑149 (MQ=255)
                                                                           tGATGGTTGCAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTGGGATAAAGAGCGTGATGGTTTCGTACTGGGCGAt                                                                    >  7:192287/1‑149 (MQ=255)
                                                                            gATGGTTGCAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACATGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTGGGATAAAGAGCGTGATGGTTTCGTACTGGGCGATg                                                                   <  2:137864/149‑1 (MQ=255)
                                                                             aTGGTTGCAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACATGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTGGGATAAAGAGCGTGATGGTTTCGTACTGGGCGATg                                                                   <  2:132415/148‑1 (MQ=255)
                                                                                gTTGCAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGCCGAGCCGCCCGTGGGATAAAGAGCGTGAGGGTTTCGTACTGGGCGATCGTGc                                                               >  6:136105/1‑149 (MQ=255)
                                                                                   gCAGGTGTCGCAGAGAAACCCAGTACGCCGCTGGGCGGTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCCCAAGCGGCGTGCCGCCCGTGGGTTAAAGAGCGTGATGGTTTCGTACTGGGCGATGGTGCCg                                                             <  8:284440/148‑1 (MQ=255)
                                                                                   gCAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTGGGATAAAGAGCGTGATGGTTTCGTACTGGGCGATGGTGCCg                                                             <  3:101196/148‑1 (MQ=255)
                                                                                    cAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATa                                                                                                                               <  1:268217/81‑1 (MQ=255)
                                                                                    cAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATa                                                                                                                               >  2:268217/1‑81 (MQ=255)
                                                                                        tGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTGGGATAAAGAGCGTGATGGTTTCGTACTGGGCGATGGTGCCGGTATGc                                                       <  3:281361/149‑1 (MQ=255)
                                                                                             cAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTGGGATAAAGAGCGTGATGGTTTCGTACTGGGCGATGGTGCCGGTATGCTGGTa                                                  <  8:384796/149‑1 (MQ=255)
                                                                                                agaAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTggg                                                                                                    >  8:100430/1‑96 (MQ=255)
                                                                                                agaAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTggg                                                                                                    <  7:100430/96‑1 (MQ=255)
                                                                                                  aaaGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTGGGATAAAGAGCGTGATGGTTTc                                                                                <  5:163208/114‑1 (MQ=255)
                                                                                                  aaaGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTGGGATAAAGAGCGTGATGGTTTc                                                                                >  6:163208/1‑114 (MQ=255)
                                                                                                   aaGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGGATTATCTACCCGCAATGATAACCCGCACGCGGCGTGCCGCCCGTGGGCTTATGAGCGTGATGGTGTCGTACTGGGCGATGGTGCCGGTATGCTGGTACTTGaa                                            <  3:267901/149‑1 (MQ=255)
                                                                                                   aaGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCTTGGGATAAAGAGCGTGATGGTTTCGTACTGGGCGATGGTGCCGGTATGCTGGTACTTGaa                                            <  6:221383/149‑1 (MQ=255)
                                                                                                      ccAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTGGGATAAAGAGCGTGATGGTTTCGTACTGGGCGATGGTGCCGGTAGGCTGGTACTGGAagct                                         >  3:182120/1‑147 (MQ=255)
                                                                                                       cAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTGGGATAAAGAGCGTGATGGTTTCGTACTGGGCGATGGTGCCGGTATGCTGGTACTTGAagag                                         >  1:256182/1‑148 (MQ=255)
                                                                                                         gTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTGGGATAAAGAGCGTGATGGTTTCGTACTGGGCGATGGTGCCGGTATGCTGGTACTTGAAGAGTAc                                      <  6:111748/149‑1 (MQ=255)
                                                                                                            cgccgcTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACGCCCAATGATAACCCGCAAGCGGCGAGCCGCCCGTGGGATAAAGAGCGTGATGGTTTCGTACTGGGCGATGGTGCCGGTATGCTTGTACTTGAAGAGTACGa                                    <  2:242156/148‑1 (MQ=255)
                                                                                                               cggtGGGCGTTGGTTGTTTTGTCGCGGCACGTGCATTATCTACCCGCAATGATACCCCGCAAGCGGCGAGCCGCCCGTGGGATAAAGAGCGTGATCGTTTCGTACTGGGCGATGGGGCCGGTATGCGGGTACTTGAAGAGTACGAacac                                <  2:231736/146‑1 (MQ=255)
                                                                                                               cgcTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTGGGATAAAGAGCGTGATGGTTTCGTACTg                                                                          <  7:376522/107‑1 (MQ=255)
                                                                                                               cgcTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTGGGATAAAGAGCGTGATGGTTTCGTACTg                                                                          >  8:376522/1‑107 (MQ=255)
                                                                                                                            tggtTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTGGGATAAAGAGCGTGATGGTTTCGTACTGGGCGATGGTGCCGGTATGCTGGTACTTGAAGAGTACGAACACGCGAAAAAAcgcg                   <  8:39688/149‑1 (MQ=255)
                                                                                                                            tggtTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTGGGATAAAGAGCGTGATGGTTTCGTACTGGGCGATGGTGCCGGTATGCTGGTACTTGAAGAGTACGAACACGCGAAAAAAcgcg                   >  3:362595/1‑149 (MQ=255)
                                                                                                                              gtTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTGGGATAAAGAGCGTGATGGTTTCGTACTGGGCGATGGTGCCGGTATGCTGGTACTTGAAGAGTACGAACACGCGAAAAAACGCgg                  <  8:192287/148‑1 (MQ=255)
                                                                                                                               ttttGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTGGGATAAAGAGCGTGATGGTTTCGTACTGGGCGATGGTGCCGGTATGCTGGTACTTGAAGAGTACGAACACGCGAAAAAACGCGGTg                <  8:113862/149‑1 (MQ=255)
                                                                                                                                             gTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTGGGATAAAGAGCGTGATGGTTTCGTACTGGGCGATGGTGCCGGTATGCTGGTACTTGAAGAGTACGAACACGCGAAAAAACGCGGTGCGAAAATTTACGCt  >  2:256558/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
TCGCGACTGCCTGTACTTCCGGCGTGCACAACATTGGCCATGCTGCGCGTATTATCGCGTATGGCGATGCTGACGTGATGGTTGCAGGTGGCGCAGAGAAAGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTGGGATAAAGAGCGTGATGGTTTCGTACTGGGCGATGGTGCCGGTATGCTGGTACTTGAAGAGTACGAACACGCGAAAAAACGCGGTGCGAAAATTTACGCT  >  NC_000913/1152417‑1152706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑