Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,254,942 G→A 6.8% S337S (TCC→TCT)  ycgV ← putative adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,254,9420GA 6.8% 184.6 / 3.1 59S337S (TCC→TCT) ycgVputative adhesin
Reads supporting (aligned to +/- strand):  ref base G (28/27);  new base A (2/2);  total (30/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.42e-01

GATAGCCAAACTGTCTCCGGTTATTCTACCGCCGCTGAGAGCCCACAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCACCGATAGTGGTTGTACCACCACGAACTTCGACGCCATTGG  >  NC_000913/1254795‑1255090
                                                                                                                                                   |                                                                                                                                                    
gATAGCCAAACTGTCTCCGGTTATTCTACCGCCGCTGAGAGCCCACAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAAccgccg                                                                                                                                                      >  2:343903/1‑148 (MQ=255)
 aTAGCGAAAATGTCTCCGGTTATACTACCGCCGCTGAGAGCCCACAAACCCCGCGCCAGACTGCAAATACGAACAACTGATATATCGGTATTGTGCATGTTGACATCACCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCgg                                                                                                                                                     <  6:82648/148‑1 (MQ=255)
        aaCTGTCTCCGGTTATTCTACCGCCGCTGAGAGCCCACAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATg                                                                                                                                             >  3:210075/1‑149 (MQ=255)
               tcCGGTTATTCTACCGCCGCTGAGAGCCCACAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTc                                                                                                                                      >  1:132881/1‑149 (MQ=255)
               tcCGGTTATTCTACCGCCGCTGAGAGCCCACAAACCCAGCGCCAGACTGCCAGTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCCCCATAAGAACCGCCGGAAAAGATGCTGTTTc                                                                                                                                      >  5:244577/1‑149 (MQ=255)
                 tggTTATTCTTCGGCCGCTGAGTGCCCACAATCCAAGCGCCAGAGTGCCATTACGATAAACTGTATTATCGGTATTTTGCATTTTGAGAACTGCCGTTGCCGTCTGGGCCGAGGCACCTTAAGAACCGGCGGAATAGATGCTGTTTc                                                                                                                                      <  4:173101/146‑1 (MQ=255)
                 cGGTTATTCTACCGCCGCTGAGAGCCCACAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTc                                                                                                                                      <  2:180776/147‑1 (MQ=255)
                 cGGTTATTCTACCGCCGCTGAGAGCCCACAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCg                                                                                                                                     >  7:176932/1‑148 (MQ=255)
                 cGGTTATTCTACCGCCGCTGAGAGCCCACAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCg                                                                                                                                     <  8:176932/148‑1 (MQ=255)
                 cGGTTATTCTACCGCCGCTGAGAGCCCACAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGt                                                                                                                                    >  2:327418/1‑149 (MQ=255)
                  ggTTATTCTACCGCCGCTGAGAGCCCACAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGtt                                                                                                                                   <  5:146186/149‑1 (MQ=255)
                    ttATTCTACCGCCGCTGAGAGCCCACAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTgc                                                                                                                                 >  7:229260/1‑149 (MQ=255)
                      aTTCTACCGCCGCTGAGAGCCCACAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTgcgc                                                                                                                               >  8:134166/1‑149 (MQ=255)
                       ttCTACCGCCGCTGAGAGCCCACAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCt                                                                                                                              <  1:74499/149‑1 (MQ=255)
                       ttCTACCGCCGCTGAGAGCCCACAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCt                                                                                                                              >  2:246722/1‑149 (MQ=255)
                                  cTGAGAGCCCACAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGtt                                                                                                                                        >  4:354968/1‑128 (MQ=255)
                                  cTGAGAGCCCACAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGtt                                                                                                                                        <  3:354968/128‑1 (MQ=255)
                                    gagaGCCCACAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCTGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGc                                                                                                                                            >  2:356453/1‑122 (MQ=255)
                                    gagaGCCCACAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGc                                                                                                                                            <  1:356453/122‑1 (MQ=255)
                                           cacaAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGACAGTAAAATTGAt                                                                                                          >  3:72921/1‑149 (MQ=255)
                                            acaAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGAt                                                                                                          >  7:231229/1‑148 (MQ=255)
                                              aaaCCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTAt                                                                                                       >  5:141040/1‑149 (MQ=255)
                                               taCCCAGCGCCAGACTGCCATTACGATCATCTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATc                                                                                                      <  1:7184/148‑1 (MQ=255)
                                                    aGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTc                                                                                                 <  1:327418/149‑1 (MQ=255)
                                                     gcgcCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCt                                                                                                <  4:210075/149‑1 (MQ=255)
                                                      cgcCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCAGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCt                                                                                                >  5:32309/1‑148 (MQ=255)
                                                                  aTTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGa                                                                                   <  6:141040/149‑1 (MQ=255)
                                                                      ccaTCAACTGTAATATCGGTCTTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATACGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGa                                                                                <  8:273374/146‑1 (MQ=255)
                                                                      cGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCAGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAg                                                                               <  6:32309/149‑1 (MQ=255)
                                                                         tCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCACTAAAATTGATAATCGCGTCTGAACCACTGGTGACGAGGc                                                                             >  8:383293/1‑148 (MQ=255)
                                                                          cAACTTTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCCAAAGAACCGCCGGAACAGATGCTGTTGCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCa                                                                           <  6:201368/149‑1 (MQ=255)
                                                                             cTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGCGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCg                                                                        >  6:62514/1‑149 (MQ=255)
                                                                               gTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGGCTGAACCACTGGTGACGAGGCCACCGcc                                                                      >  5:45611/1‑149 (MQ=255)
                                                                                  atatCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTg                                                                   >  5:70024/1‑149 (MQ=255)
                                                                                   tatCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTgc                                                                  >  7:121270/1‑149 (MQ=255)
                                                                                    atCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGa                                                                                                           <  1:200804/107‑1 (MQ=255)
                                                                                    atCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGa                                                                                                           >  2:200804/1‑107 (MQ=255)
                                                                                         tATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGgaa                                                            <  2:132881/149‑1 (MQ=255)
                                                                                         tATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGGTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGgaa                                                            <  1:246722/149‑1 (MQ=255)
                                                                                                cATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAatat                                                     <  8:229260/149‑1 (MQ=255)
                                                                                                cATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAatat                                                     <  7:134166/149‑1 (MQ=255)
                                                                                                   gTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCGGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGc                                                  >  2:35136/1‑149 (MQ=255)
                                                                                                         aacaGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTg                                            >  1:190257/1‑149 (MQ=255)
                                                                                                         aacaGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTg                                            >  8:22620/1‑149 (MQ=255)
                                                                                                         aacaGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCAGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATc                                                                                                      <  8:63279/91‑1 (MQ=255)
                                                                                                         aacaGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCAGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATc                                                                                                      >  7:63279/1‑91 (MQ=255)
                                                                                                               cGTTGCCGTCTGGGCCGAGGCACCATAAGATCCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACTAGGCCACCGCCCTTCGCGGAAGAAATATGGCTATCTGCACCGa                                      <  7:383293/149‑1 (MQ=255)
                                                                                                               cGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCACCGa                                      <  1:343903/149‑1 (MQ=255)
                                                                                                                 ttGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAAt                                                                                                              >  4:95386/1‑75 (MQ=255)
                                                                                                                 ttGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAAt                                                                                                              <  3:95386/75‑1 (MQ=255)
                                                                                                                      gTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCACCGATAGTGGt                               <  8:121270/149‑1 (MQ=255)
                                                                                                                       tCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCACCGATAGTGGtt                              <  4:72921/149‑1 (MQ=255)
                                                                                                                         tGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCACCGATAGTGGTTg                             >  7:175995/1‑148 (MQ=255)
                                                                                                                          gggCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCACCGATAGTGGTTGTa                           >  7:262052/1‑149 (MQ=255)
                                                                                                                               gAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCACCGATAGTGGTTGTaccacc                      <  8:231229/149‑1 (MQ=255)
                                                                                                                               gAGGCACCATAAGAACCGCCGGAAAAGATGCTGTGTCGTTGCGCTGCCGTGCCAGAAAAATTGATTATCGCGTCTGAACCACTGTTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCACCGATAGTGGTTGTaccacc                      <  4:10839/149‑1 (MQ=255)
                                                                                                                                      cATCAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTAATTATCGCGTCTGAAACACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCAACGATAGAGGTTGTACCACCACGAAc                >  1:18993/1‑148 (MQ=255)
                                                                                                                                      cATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCACCGATAGTGGTTGTACCACCACGAACt               >  7:390871/1‑149 (MQ=255)
                                                                                                                                       aTAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCACCGATAGTGGTTGTACCACCACGAACtt              >  2:194282/1‑149 (MQ=255)
                                                                                                                                        tAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCACCGATAGTGGTTGTACCACCACGAACTTc             <  6:45611/149‑1 (MQ=255)
                                                                                                                                           gAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCACCGATAGTGGTTGTACCACCACGAACTTCGAc          <  6:70024/149‑1 (MQ=255)
                                                                                                                                                   ggAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCACCGATAGTGGTTGTACCACCACGAACTTCGACGCCATTgg  >  8:7151/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GATAGCCAAACTGTCTCCGGTTATTCTACCGCCGCTGAGAGCCCACAAACCCAGCGCCAGACTGCCATTACGATCAACTGTAATATCGGTATTTTGCATGTTGACAACAGCCGTTGCCGTCTGGGCCGAGGCACCATAAGAACCGCCGGAAAAGATGCTGTTTCGTTGCGCTGCCGTGCCAGTAAAATTGATTATCGCGTCTGAACCACTGGTGACGAGGCCACCGCCCTGCGCGGAAGAAATATGGCTATCTGCACCGATAGTGGTTGTACCACCACGAACTTCGACGCCATTGG  >  NC_000913/1254795‑1255090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑