Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,291,117 G→T 7.0% Q292H (CAG→CAT)  rssB → PcnB‑degradosome interaction factor; response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,291,1170GT 7.0% 164.1 / 4.0 57Q292H (CAG→CAT) rssBPcnB‑degradosome interaction factor; response regulator
Reads supporting (aligned to +/- strand):  ref base G (26/27);  new base T (2/2);  total (28/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.25e-01

CGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGATGTTGTCTGCAGAATGAGCAAACGA  >  NC_000913/1290969‑1291263
                                                                                                                                                    |                                                                                                                                                  
cgTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAg                                                                                                                                                    <  6:310596/149‑1 (MQ=255)
        aaGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGTCGTTAAATACCGGCGAACACCAGGTGCAAAt                                                                                                                                            <  1:78020/149‑1 (MQ=255)
        aaGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATAGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCaaa                                                                                                                                             >  5:99144/1‑148 (MQ=255)
                 aaCCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATgg                                                                                                                                   >  7:255779/1‑149 (MQ=255)
                    cATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAAt                                                                                                                                            >  4:70723/1‑137 (MQ=255)
                    cATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAAt                                                                                                                                            <  3:70723/137‑1 (MQ=255)
                    cATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGgtgt                                                                                                                                >  6:212432/1‑149 (MQ=255)
                     aTTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGtt                                                                                                                               <  6:99144/149‑1 (MQ=255)
                      tttACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTc                                                                                                                              <  8:255779/149‑1 (MQ=255)
                          cTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGtt                                                                                                                          <  6:199842/149‑1 (MQ=255)
                                  ggCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACtt                                                                                                                  >  3:39946/1‑149 (MQ=255)
                                        tCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTATGAATGCGACGTTAAATACCGGCGAACAACAGGTGCAAATCa                                                                                                                                          >  1:338691/1‑119 (MQ=255)
                                          tGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAAc                                                                                                          >  8:246057/1‑149 (MQ=255)
                                           gCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACAAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTAAGGTAAc                                                                                                          >  6:321998/1‑148 (MQ=255)
                                                ggCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTAt                                                                                                    <  8:227764/149‑1 (MQ=255)
                                                ggCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTAt                                                                                                    >  1:166683/1‑149 (MQ=255)
                                                       tCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGTGTCTGAATGCTACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAAt                                                                                              <  3:365839/148‑1 (MQ=255)
                                                         cGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTACCGTTAGGCACTTTAGGTAACGCTTATTTGAATCaa                                                                                           <  5:244351/149‑1 (MQ=255)
                                                              ttAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTgg                                                                                       <  5:212432/148‑2 (MQ=255)
                                                                 gTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGAGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGc                                                                                     >  1:186840/1‑147 (MQ=255)
                                                                       tattatCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAgcgatg                                                                             >  1:316224/1‑149 (MQ=255)
                                                                         atatCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTa                                                                                                     <  6:250673/122‑1 (MQ=255)
                                                                           atcatcGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAgcgatgcgat                                                                         >  7:162720/1‑149 (MQ=255)
                                                                            tcatcGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAgcgatgcgat                                                                         <  4:37871/148‑1 (MQ=255)
                                                                            tcatcGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGGCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGcgatgcgatg                                                                        >  6:189852/1‑149 (MQ=255)
                                                                               tcGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCt                                                                     <  8:162720/149‑1 (MQ=255)
                                                                                         aaaaaTCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAAt                                                                                          <  3:181038/118‑1 (MQ=255)
                                                                                         aaaaaTCTCATTCTGGTTTCTGCGGGACTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAAt                                                                                          >  4:181038/1‑118 (MQ=255)
                                                                                          aaaaTCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACAGGCGAACACAAGGTGAAAATCAGTAATGGTGTTACTTTAGGCACTTTAGGTAACGCTTATTAGAATCAATTGAGCCAgcgatg                                                                             >  1:157151/1‑130 (MQ=255)
                                                                                                  aTTCTGGTTTCTGCGGGTCTGAATGCGACTTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATgg                                                    <  6:250448/147‑1 (MQ=255)
                                                                                                  aTTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAgcgatgcga                                                                          <  1:376178/125‑1 (MQ=255)
                                                                                                  aTTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAgcgatgcga                                                                          >  2:376178/1‑125 (MQ=255)
                                                                                                  aTTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATggg                                                   <  4:39946/148‑1 (MQ=255)
                                                                                                  aTTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATgg                                                    <  2:166683/147‑1 (MQ=255)
                                                                                                   ttCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAg                                                                                                                        >  4:217852/1‑78 (MQ=255)
                                                                                                   ttCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAg                                                                                                                        <  3:217852/78‑1 (MQ=255)
                                                                                                    tCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCATGTGCAAATCAg                                                                                                                                         >  1:220676/1‑60 (MQ=255)
                                                                                                    tCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCATGTGCAAATCAg                                                                                                                                         >  1:226557/1‑60 (MQ=255)
                                                                                                    tCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCATGTGCAAATCAg                                                                                                                                         <  2:220676/60‑1 (MQ=255)
                                                                                                    tCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCATGTGCAAATCAg                                                                                                                                         <  2:226557/60‑1 (MQ=255)
                                                                                                         tttCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCggt                                           <  2:316224/149‑1 (MQ=255)
                                                                                                                     tGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATgg                                                    >  8:150104/1‑128 (MQ=255)
                                                                                                                     tGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATgg                                                    <  7:150104/128‑1 (MQ=255)
                                                                                                                            gACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCggtg                                          >  1:145750/1‑131 (MQ=255)
                                                                                                                            gACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCggtg                                          <  2:145750/131‑1 (MQ=255)
                                                                                                                            gACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGAtgt                        >  3:179379/1‑149 (MQ=255)
                                                                                                                              cGTTAAATACCGGCGACCACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACGTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCGGTGGTCGCCTGCGCTTGAtgttg                      >  2:70917/1‑149 (MQ=255)
                                                                                                                              cGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGCTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCGGTGGTCGCCTGCGCTTGatgatg                      >  4:311750/1‑149 (MQ=255)
                                                                                                                               gTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGAtgttgt                     >  5:24046/1‑149 (MQ=255)
                                                                                                                                  aaaTACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGATGTTGTCt                   <  7:246057/148‑1 (MQ=255)
                                                                                                                                  aaaTACAGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCGTGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGATGTTGTCt                   <  3:266575/148‑1 (MQ=255)
                                                                                                                                   aaTACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGATGTTGTCTGc                 <  5:189852/149‑1 (MQ=255)
                                                                                                                                   aaTACCGGCGAACACCAGGTGCAAATCAGTAAGGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGTGCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGATGTTGTCTGc                 <  5:321998/149‑1 (MQ=255)
                                                                                                                                    aTACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGATGTTGTCTGCa                <  6:24046/149‑1 (MQ=255)
                                                                                                                                        cGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGATGTTGTCTGCAGAAt            >  2:38884/1‑149 (MQ=255)
                                                                                                                                                acCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCt                                                                     <  6:360407/84‑1 (MQ=255)
                                                                                                                                                acCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCt                                                                     >  5:360407/1‑84 (MQ=255)
                                                                                                                                                  cAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGATGTTGTCTGCAGAATGAGCAAACGa  >  6:146392/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGATGTTGTCTGCAGAATGAGCAAACGA  >  NC_000913/1290969‑1291263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑