| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,708,854 | C→G | 29.2% | Q646E (CAA→GAA) | rsxC → | SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,708,854 | 0 | C | G | 29.2% | 13.0 / 9.9 | 17 | Q646E (CAA→GAA) | rsxC | SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex |
| Reads supporting (aligned to +/- strand): ref base C (6/6); new base G (2/3); total (8/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.30e-01 | |||||||||||
CAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCG > NC_000913/1708712‑1708998 | cAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAGCGCGCTATAGCCCCAGCCACAGCACCCCACCTGGACCCGCATCACGCTAATCCCGAGCCCCAacaacaa > 5:361496/1‑147 (MQ=14) aCAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGGTATTGCACGTGCCAGTGCGCGAAAGCTGGAACAGCACCAGGCTAATGCCGAGGGAGCCCAACAAGTAGATACGCGCAAAgccg > 8:259795/1‑149 (MQ=11) acgCTAATGCGGAACCATAAGAACACGTCGATCCGCTCAAAGCCGCCGTCGAAGCCGCTATAGCCCGTGCCAAAGCACGCAAGCTGTAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGaa < 7:259795/147‑1 (MQ=14) gCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCACGCAAGCTGGAGCAGCAAAAGGCGACTGCCTAGCAAGAACAAAAGGTCGCACAGCGCAAAGCCGCCGTCGAAGc > 6:314817/1‑149 (MQ=14) cgcgCAAAGCCGCCGTCGAAGCCGCTATGGCCCGAGCCACAGCACTCAAGCTGGCACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACgg < 5:314817/149‑1 (MQ=14) cTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATcc < 8:160732/73‑1 (MQ=255) aaGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAg < 7:176435/93‑1 (MQ=12) cAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTAGCTCCGCGCAAAGCTGCCg > 2:6941/1‑148 (MQ=255) gCTAATGCCGAGCCAGAACAACAGGTCGACCCGCTCAAAGCCGCAGTCGAAGCCGCTATTGCCCGAGCCAAAGCTCTCTACCGGGAACAGGAACTGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGc < 3:334153/137‑1 (MQ=14) tAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCgg > 8:43903/1‑149 (MQ=255) aTGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACTGGAACAGCAACCGGCTAATGCGGAg > 8:368334/1‑104 (MQ=11) aTGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAg < 7:368334/104‑1 (MQ=255) cGAGCAAGAACAACACGACGATCCCCTCAAAGCCGCCTTCGAAGACGAGATTTCAAGAGACAAAGCGCGCAACCTGGAACAGCAACAGGCTAATGCGGATCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTg < 1:197578/149‑1 (MQ=11) aaccAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAAc < 6:78647/82‑1 (MQ=255) aaccAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAAc > 5:78647/3‑84 (MQ=255) cAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGaa < 4:113564/74‑1 (MQ=255) cAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGaa > 3:113564/1‑74 (MQ=255) aGAACAACAGGTCGATCCGCGAAAAGCCGAAGTCGCAGCCGCTATTGCACGAGCAAAAGCAATAAAACGGCCACAGCAACAGGATAAATCGGACCCAGAAGAACAGGGTTATCCGCAAAAAGCTTAAGTAGCCTCGGCTATTGCAagcg > 2:174037/1‑149 (MQ=2) | CAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCG > NC_000913/1708712‑1708998 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |