Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,724,298 T→C 8.5% N120D (AAT→GAT)  sodC ← superoxide dismutase, Cu, Zn, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,724,2980TC 8.5% 132.0 / 3.8 48N120D (AAT→GAT) sodCsuperoxide dismutase, Cu, Zn, periplasmic
Reads supporting (aligned to +/- strand):  ref base T (23/20);  new base C (2/2);  total (26/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.66e-01

GCATAGCGTTCACCGCCACCGCCCAGCGGTTTAGGTTGATCGGACATATTATCGCCGCCAACGTGGACCATCAGCGCTTTGTCTTTGATTTCATCCAGTGATTTCAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCATTATTGACGACCAGTGCAGGCAGATCGCCTAAATGCCCGGCACCTTCTGGCCCTTCATGTTTACCGGTATTTTGTGGATCAAGATGCCCGCCTGCGGATTCCGCGGCGCTGGCTTTGCCATCTTTGGTGGCTGGCTGGCAGCTTCCTTTG  >  NC_000913/1724153‑1724446
                                                                                                                                                 |                                                                                                                                                    
gCATAGCGTTCACCGCCACCGCCCAGCGGTTTAGGTTGATCGGACATATTATCGCCGCCAACGTGGACCATCAGCGCTTTGTCTTTGATTTCATCCAGTGATTTCAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCattatt                                                                                                                                                   <  1:14051/149‑1 (MQ=255)
gCATAGCGTTCACCGCCACCGCCCAGCGGTTTAGGTTGATCGGACATATTATCGCCGCCAACGTGGACCATCAGCGCTTTGTCTTTGATTTCATCCAGTGATTTCAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCattatt                                                                                                                                                   >  5:102408/1‑149 (MQ=255)
  aTAGCGTTCACCGCCACCGCCCAGCGGTTTAGGTTGATCGGACATATTATCGCCGCCAACGTGGACCATCAGCGCTTTGTCTTTGATTTCATCCAGTGATTTCAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCATTATTga                                                                                                                                                 <  6:194152/149‑1 (MQ=255)
     gCGTTCACCGCCACCGCCCAGCGGTTTAGGTTGATCGGACATATTATCGCCGCCAACGTGGACCATCAGCGCTTTGTCTTTGATTTCATCCAGTGATTTCAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCATTATTgacga                                                                                                                                              <  1:26028/149‑1 (MQ=255)
     gCGTTCACCCCCACCGCCCAGCGGTTTAGGTTGATCGGACATATTATCGCCGCCAACGACGACCATCAGCCCAATGACATTGATTACAACCACTGATTTCAGACCAGGCCCCATGACGGCAACGGTAGCTTTGCCGTCATAATTgcctc                                                                                                                                              >  2:319642/1‑145 (MQ=255)
          caccgccaccgcCCAGCGGTTTAGGTTGATCGGACATATTATCGCCGCCAACGTGGACCATCAGCGCTTTGTCTTTGATTTCATCCAGTGATTTCAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCATTATTGACGACCAGt                                                                                                                                         >  4:231305/1‑149 (MQ=255)
           accgccaccgccCAGCGGTTTAGGTTGATCGGACATATTATCGCCGCCAACGTGGACCATCAGCGCTTTGTCTTTGATTTCATCCAGTGATTTCAGACGAGGCGCGATGACGGCATCCGTAGCTTTGCCGTCATTATTGACGACCAGTg                                                                                                                                        >  1:110955/1‑149 (MQ=255)
            ccgccaccgccCAGCGGTTTAGGTTGATCGGACATATTATCGCCGCCAACGTGGACCATCAGCGCTTTGTCTTTGATTTCATCCAGTGATTTCAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCATTATTgacgac                                                                                                                                             >  6:102651/1‑143 (MQ=255)
            ccgccaccgccCAGCGGTTTAGGTTGATCGGACATATTATCGCCGCCAACGTGGACCATCAGCGCTTTGTCTTTGATTTCATCCAGTGATTTCAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCATTATTgacgac                                                                                                                                             <  5:102651/143‑1 (MQ=255)
                ctccgcgCTGCGGTTTTGTTTTATGGGACTTATTATCGCGGGCATCGTGGAGGATCAGCGGTTTGTCTTTGATTTCATCCAGTGATTTCAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCATTATTGACGACCTGTgcaggg                                                                                                                                   <  1:319642/147‑2 (MQ=255)
                      ccAGCGGTTTAGGTTGATCGGACATATTATCGCCGCCAACGTGGACCATCAGCGCTTTGTCTTTGATTTCATCCAGTGATTTCAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCATTATTGACGACCAGTGCAGGCAGATCg                                                                                                                             >  1:292419/1‑149 (MQ=255)
                        aGCGGTTTAGGTTGATCGGACATATTATCGCCGCCAACGTGGACCATCAGCGCTTTGTCTTTGATTTCATCCAGTGATTTCAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCATCATTGACGACCAGTGCAGGCAGATCGcc                                                                                                                           >  6:144686/1‑149 (MQ=255)
                        aGCGGTTTAGGTTGATCGGACATATTATCGCCGCCAACGTGGACCATCAGCGCTTTGTCTTTGATTTCATCCAGTGATTTCAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCATCATTGACGACCAGTGCAGGCAGATCGcc                                                                                                                           >  6:305775/1‑149 (MQ=255)
                                aGGTTGATCGGACATATTATCGCCGCCAACGTGGACCATCAGCGCTTTGTCTTTGATTTCATCCAGTGATTTCAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCATTATTGACGACCAGTGCAGGCAGATCGCCTAAATGcc                                                                                                                   >  5:332781/1‑149 (MQ=255)
                                   ttGATCGGACATATTATCGCCGCCAACGTGGACCATCAGCGCTTTGTCTTTGATTTCATCCAGTGATTTCAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCATTATTGACGACCAGTGCAGGCAGATCGCCTAAATGCCCgg                                                                                                                <  1:172801/149‑1 (MQ=255)
                                    tGATCGGACATATTATCGCCGCCAACGTGGACCATCAGCGCTTTGTCTTTGATTTCATCCAGTGATTTCAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCATTATTGACGACCAGTGCAGGCAGATCGCCTAAATGCCCgg                                                                                                                >  2:382973/1‑148 (MQ=255)
                                         ggACATATTATCGCCGCCAACGTGGACCATCAGCGCTTTGTCTTTGATTTCATCCAGTGATTTCAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCATTATTGACGACCAGTGCAGGCAGATCGCCTAAATGCCCGGCACCtt                                                                                                          >  5:266335/1‑149 (MQ=255)
                                          gACATATTATCGCCGCCAACGTGGACCATCAGCGCTTTGTCTTTGATTTCATCCAGTGATTTCAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCATTATTGACGACCAGTGCAGGCAGATCGCCTAAATGCCCGGCACCTTc                                                                                                         >  4:42488/1‑149 (MQ=255)
                                            cATATTATCGCCGCCAACGTGGACCATCAGCGCTTTGTCTTTGATTTCATCCAGTGATTTCAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCATTATTGACGACCAGTGCAGGCAGATCGCCTAAATGCCCGGCACCTTCTg                                                                                                       <  3:231305/149‑1 (MQ=255)
                                            cATATTATCGCCGCCAACGTGGACCATCAGCGCTTTGTCTTTGATTTCATCCAGTGATTTCAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCATTATTGACGACCAGTGCAGGCAGATCGCCTAAATGCCCGGCACCTTCTg                                                                                                       >  8:267806/1‑149 (MQ=255)
                                             atatTATCGCCGCCAACGTGGACCATCAGCGCTTTGTCTTTGATTTCATCCAGTGATTTCAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCATTATTGACGACCAGTGCAGGCAGATCGCCTAAATGCCCGGCACCTTCTgg                                                                                                      >  1:363191/1‑149 (MQ=255)
                                                   tCGCCGCCAACGTGGACCATCAGCGCTTTGTCTTTGATTTCATCCAGTGATTTCAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCATTATTGACGACCAGTGCAGGCAGATCGCCTAAATGCCCGGCACCTTCTGGCCCtt                                                                                                 >  7:66359/1‑148 (MQ=255)
                                                   tCGCCGCCAACGTGGACCATCAGCGCTTTGTCTTTGATTTCATCCAGTGATTTCAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCATTATTGACGACCAGTGCAGGCAGATCGCCTAAATGCCCGGCACCTTCTGGCCCTTc                                                                                                <  6:362408/149‑1 (MQ=255)
                                                     gccgccAACGTGGACCATCAGCGCTTTGTCTTTGATTTCATCCAGTGATTTCAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCATCATTGACGACCAGTGCAGGCAGATCGCCTAAATGCCCGGCACCTTCTGGCCCTTCAt                                                                                              <  5:305775/149‑1 (MQ=255)
                                                     gccgccAACGTGGACCATCAGCGCTTTGTCTTTGATTTCATCCAGTGATTTCAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCATCATTGACGACCAGTGCAGGCAGATCGCCTAAATGCCCGGCACCTTCTGGCCCTTCAt                                                                                              <  5:144686/149‑1 (MQ=255)
                                                                      tCAGCGCTTTGTCTTTGATTTCATCCAGTGATTTCAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCATTATTGACGACCAGTGCAGGCAGATCGCCTAAATGCCCGGCACCTTCTGGCCCTTCATGTTTACCGGTATTTtgt                                                                             >  7:348946/1‑149 (MQ=255)
                                                                        agggcTTTGTCTTTGATTTCATCCAGTGATTTCAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCATTATTGACGACCAGTGCAGGCAGATCACCTAAATGCCCGTCACCTTCTGGCCCTTCATGTTTACCGGTATTTtgtg                                                                            <  1:267835/145‑1 (MQ=255)
                                                                        aGCGCTTTGTCTTTGATTTCATCCAGTGATTTCAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCATTATTGACGACCAGTGCAGGCAGATCGCCTAAATGCCCGGCACCTTCTGGCCCTTCATGTTTACCGGTATTTtgtg                                                                            <  6:102408/148‑1 (MQ=255)
                                                                        aGCGCTTTGTCTTTGATTTCATCCAGTGATTTCAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCATTATTGACGACCAGTGCAGGCAGATCGCCTAAATGCCCGGCACCTTCTGGCCCTTCATGTTTACCGGTATTTtgtg                                                                            <  1:382973/148‑1 (MQ=255)
                                                                        aGCGCTTTGTCTTTGATTTCATCCAGTGATTTCAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCATTATTGACGACCAGTGCAGGCAGATCGCCTAAATGCCCGGCACCTTCTGGCCCTTCATGTTTACCGGTATTTtgtg                                                                            <  6:266335/148‑1 (MQ=255)
                                                                        aGCGCTTTGTCTTTGATTTCATCCAGTGATTTCAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCATTATTGACGACCAGTGCAGGCAGATCACCTAAATGCCCGGCACCTTCTGGCACATCATGGTTACAGGTATTTtgtg                                                                            >  2:267835/1‑148 (MQ=255)
                                                                         gcgcTTTGTCTTTGATTTCATCCAGTGATTTCAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCATTATTGACGACCAGTGCAGGCAGATCGCCCAAATGCCCGGCACCTTCTGGCCCTTCATGTTTACCGGTATTTTGTGGa                                                                          >  7:149339/1‑149 (MQ=255)
                                                                               tGTCTTTGATTTCATCCAGTGATTTCAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCATTATTGACGACCAGTGCAGGCAGATCGCCTAAATGCCCGGCACCTTCAGGCCCTTCATGTTTACACGTATTTTGTGGATc                                                                        >  1:310414/1‑145 (MQ=255)
                                                                                   tttGATTTCATCCAGTGATTTCAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCATTATTGACGACCAGTGCAGGCAGATCGCCTAAATGCCCGGCACCTTCTGGCCCTTCATGTTTACCGGTATTTTGTGGATCAAGATGcc                                                                >  6:129231/1‑149 (MQ=255)
                                                                                      gATTTCATCCAGTGATTTCAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCATTATTGACGACCAGTGCAGGCAGATCGCCTAAATGCCCGGCACCTTCTGGCCCTTCATGTTTACCGGTATTTTGTGGATCAAGATGCCCGc                                                             >  4:323277/1‑149 (MQ=255)
                                                                                             tCCAGTGATTTCAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCATTATTGACGACCAGTGCAGGCAGATCGCCTAAATGCCCGGCACCTTCTGGCCCTTCATGTTTACCGGTATTTTGTGGATCAAGATGCCCGCCTGCGGa                                                      >  3:278725/1‑149 (MQ=255)
                                                                                                     tttCAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCATTATTGACGACCAGTGCAGGCAGATCGCCCAAATGCCCGGCACCTTCTGGCCCTTCATGTTTACCGGTATTTTGTGGATCAAGATGCCCGCCTGCGGATTCCgcgg                                              <  8:149339/149‑1 (MQ=255)
                                                                                                        cAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCATTATTGACGACCAGTGCAGGCAGATCGCCTAAATGCCCGGCACCTTCTGGCCCTTCATGTTTACCGGTATTTTGTGGATCAAGATGCCCGCCTGCGGATTCCGCGgcgc                                           >  3:182731/1‑149 (MQ=255)
                                                                                                             gAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCATTATTGACGACCAGTGCAGGCAGATCGCCTAAATGCCCGGCACCTTCTGGCCCTTCATGTTTACCGGTATTTTGTGGATCAAGATGCCCGCCTGCGGATTCCGCGGCGCTGGCt                                      >  2:205697/1‑149 (MQ=255)
                                                                                                               ggCGCGATGACGGCATCGGTAGCTTTGCCGTCATTATTGACGACCAGTGCAGGCAGATCGCCTAAATGCCCGGCACCTTCTGGCCCTTCATGTTTACCGGTATTTTGTGGATCAAGATGCCCGCCTGCGGATTCCGCGGCGCTGGCttt                                    <  3:42488/149‑1 (MQ=255)
                                                                                                                    gATGACGGCATCGGTAGCTTTGCCGTCATTATTGACGACCAGTGCAGGCAGATCGCCTAAATGCCCGGCACCTTCTGGCCCTTCATGTTTACCGGTATTTTGTGGATCAAGATGCCCGCCTGCGGATTCCGCGGCGCTGGCTTTGCCAt                               >  6:266761/1‑149 (MQ=255)
                                                                                                                          ggCCTCGGTAGCTTCGCCGTCATTACTGACGACCAGTGCAGGCCGCGCCCCTAAATGCCCGGCACCTGCTCCCCCTCCATGCCTACCGGGAGCTTCTGGCTCCAGATGCCCGCCTGCGGCTGCCGCGGCGCTGGCTTTGCCATCTTtgg                         <  8:348946/149‑1 (MQ=255)
                                                                                                                          ggCATCGGTAGCTTTGCCGTCATTATTGACGACCAGTGCAGGCAGATCGCCTAAATGCCCGGCACCTTCTGGCCCTTCATGTTTACCGGTATTTTGTGGATCAAGATGCCCGCCTGCGGATTCCGCGGCGCTGGCTTTGCCATCTTtgg                         >  8:157313/1‑149 (MQ=255)
                                                                                                                            cATCGGTAGCTTTGCCGTCATTATTGACGACCAGTGCAGGCAGATCGCCTAAATGCCCGGCACCTTCTGGCCCTTCATGTTTACCGGTATTTTGTGGATCAAGATGCCCGCCTGCGGATTCCGCGGCGCTGGCTTTGCCATCTTtggtg                       <  2:292419/149‑1 (MQ=255)
                                                                                                                            cATCGGTAGCTTTGCCGTCATTATTGACGACCAGAGCAGGCAGATCGCCTAAATGCCCGGCACCTTCTGGCCCTTCATGTATACCGCTATTTTGTGGATCAAGATGCCCGCCTGCGGATTCCGCGGCGCTGGCTTTGCCATCTTtggtg                       >  4:187246/1‑149 (MQ=255)
                                                                                                                              tCGGTAGCTTTGCCGTCATTATTGACGACCAGTGCAGGCAGATCGCCTAAATGCCCGGCACCTTCTGGCCCTTCATGTTTACCGGTATTTTGTGGATCAAGATGCCCGCCTGCGGATTCCGCGGCGCTGGCTTTGCCATCTTTGGtggc                     <  4:182731/149‑1 (MQ=255)
                                                                                                                                ggTAGCTTTGCCGTCATTATTGACGACCAGTGCAGGCAGATCGCCTAAATGCCCGGCACCTTCTGGCCCTTCATGTTTACCGGTATTTTGTGGATCAAGATGCCCGCCTGCGGATTCCGCGGCGCTGGCTTTGCCATCTTTGGtggct                    <  5:266761/148‑1 (MQ=255)
                                                                                                                                     cTTTGCCGTCATTATTGACGACCAGTGCAGGCAGATCGCCTAAATGCCCGGCACCTTCTGGCCCTTCATGTTTACCGGTATTTTGTGGATCAAGATGCCCGCCTGCGGATTCCGCGGCGCTGGCTTTGCCATCTTTGGtggctggctgg              <  1:205697/149‑1 (MQ=255)
                                                                                                                                        tGCCGTCATTATTGACGACCAGTGCAGGCAGATCGCCTAAATGCCCGGCACCTTCTGGCCCTTCATGTTTACCGGTATTTTGTGGATCAAGATGCCCGCCTGCGGATTCCGCGGCGCTGGCTTTGCCATCTTTGGtggctggctggc             <  6:332781/147‑1 (MQ=255)
                                                                                                                                            gTCATTATTGACGACCAGTGCAGGCAGATCGCCTAAATGCCCGGCACCTTCTGGCCCTTCATGTTTACCGGTATTTTGTGGATCAAGATGCCCGCCTGCGGATTCCGCGGCGCTGGCTTTGCCATCTTTGGTGGCTGGCTGGCAGCTTc       <  5:129231/149‑1 (MQ=255)
                                                                                                                                               attattGACGACCAGTGCAGGCAGATCGCCTAAATGCCCGGCACCTTCTGGCCCTTCATGTTTACCGGTATTTTGTGGATCAAGATGCCCGCCTGCGGATTCCGCGGCGCTGGCTTTGCCATCTTTGGTGGCTGGCTGGCAGCTTCCtt    >  5:333410/1‑149 (MQ=255)
                                                                                                                                               attattGACGACCAGTGCAGGCAGATCGCCTAAATGCCCGGCACCTTCTGGCCCTTCATGTTTACCGGTATTTTGTGGATCAAGATGCCCGCCTGCGGATTCCGCGGCGCTGGCTTTGCCATCTTTGGTGGCTGGCTGGCAGCTTCCtt    <  2:363191/149‑1 (MQ=255)
                                                                                                                                                 tattGACGACCAGTGCAGGCAGATCCCCTAAATGCCCGGCACCTTCTGGCCCTTCATGTTTAGAGGTATTTTGTGGATCAAGATGCACGCCTGCGGATTCCGCGGCGCTGGCTTTGCCATCTTTGGTGGCTGGCTGGCAGCTTCCTTTg  >  8:141339/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GCATAGCGTTCACCGCCACCGCCCAGCGGTTTAGGTTGATCGGACATATTATCGCCGCCAACGTGGACCATCAGCGCTTTGTCTTTGATTTCATCCAGTGATTTCAGACGAGGCGCGATGACGGCATCGGTAGCTTTGCCGTCATTATTGACGACCAGTGCAGGCAGATCGCCTAAATGCCCGGCACCTTCTGGCCCTTCATGTTTACCGGTATTTTGTGGATCAAGATGCCCGCCTGCGGATTCCGCGGCGCTGGCTTTGCCATCTTTGGTGGCTGGCTGGCAGCTTCCTTTG  >  NC_000913/1724153‑1724446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑