Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,752,392	T→C	7.5%	Q480R (CAG→CGG)	ydhV ←	putative oxidoreductase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,752,392	0	T	C	7.5%	157.7 / 3.7	53	Q480R (CAG→CGG)	ydhV	putative oxidoreductase subunit
Reads supporting (aligned to +/- strand):  ref base T (22/27);  new base C (2/2);  total (24/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

TTTGGTTTCATCGTAAGCATCTTCAGAACCAAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCAC  >  NC_000913/1752244‑1752533                                                                                                                                                     |                                                                                                                                              tttGGTTTCATCGTAAGCATCTTCAGAACCAAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCt                                                                                                                                               >  7:116011/1‑149 (MQ=255)     gTTTCATCGTAAGCATCTTCAGAACCAAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCTgcgc                                                                                                                                           <  7:158518/149‑1 (MQ=255)     gTTTCATCGTAAGCATCTTCAGAACCAAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCTgcgc                                                                                                                                           <  6:211533/149‑1 (MQ=255)        tCATCGTAAGCATCTTCAGAACCAAAAAGTTCTTTCGCCACTTCCCGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCTGCGCTGa                                                                                                                                        >  3:44242/1‑149 (MQ=255)        tCATCGTAAGCATCTTCAGAACCAAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCTGCGCTGa                                                                                                                                        >  6:328503/1‑149 (MQ=255)              tAAGCATCTTCAGAACCAAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCTGCGCTGACGCtt                                                                                                                                   >  4:192364/1‑148 (MQ=255)                  cATCTTCAGAACCAAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCTGCGCTGACGCTTCATTg                                                                                                                              <  5:328503/149‑1 (MQ=255)                   aTCTTCAGAACCAAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCTGCGCTGACGCTTCATTg                                                                                                                              >  3:272315/1‑148 (MQ=255)                    tCTTCAGAACCAAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCTGCGCTGACGCTTCATTgg                                                                                                                             >  8:279602/1‑148 (MQ=255)                      ttCAGAACCAAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCAt                                                                                                                          >  5:194062/1‑149 (MQ=255)                        cAGAACCAAAAATTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCTGCGCTGACGGTTCATTGGCATg                                                                                                                         <  8:302158/148‑1 (MQ=255)                          gAACCAAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTg                                                                                                                      <  4:44242/149‑1 (MQ=255)                             ccAAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAAc                                                                                                                   <  1:201406/149‑1 (MQ=255)                              cAAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAAcc                                                                                                                  >  1:296598/1‑149 (MQ=255)                                   aGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATa                                                                                                             <  8:51897/149‑1 (MQ=255)                                   aGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATa                                                                                                             <  7:97339/149‑1 (MQ=255)                                          tCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCa                                                                                                         <  5:333135/146‑1 (MQ=255)                                               aCTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGa                                                                                                 >  8:205220/1‑149 (MQ=255)                                                cTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGAc                                                                                                <  8:219912/149‑1 (MQ=255)                                                     cGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCagag                                                                                           <  8:116011/149‑1 (MQ=255)                                                       ttGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGtt                                                                                         >  3:293475/1‑149 (MQ=255)                                                            gTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTAttt                                                                                    >  2:254721/1‑149 (MQ=255)                                                            gTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTAttt                                                                                    <  7:220993/149‑1 (MQ=255)                                                             tttCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTAtttt                                                                                   >  4:343220/1‑149 (MQ=255)                                                               tCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTc                                                                                 >  6:36948/1‑149 (MQ=255)                                                                 aaTGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTcgc                                                                               <  7:279602/149‑1 (MQ=255)                                                                       aaGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGcc                                                                         <  4:272315/149‑1 (MQ=255)                                                                        aGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGccc                                                                        <  6:194062/149‑1 (MQ=255)                                                                         gCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGccc                                                                        >  8:341060/1‑148 (MQ=255)                                                                                  aaTAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGTACATACAGTTAACAATGGAACCGACCCGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCaga                                                                                            >  5:151916/1‑119 (MQ=255)                                                                                  aaTAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGCCCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCtt                                                              >  4:295853/1‑149 (MQ=255)                                                                                  aaTAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCCGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCaga                                                                                            <  6:151916/119‑1 (MQ=255)                                                                                  aaTAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCCGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCaga                                                                                            <  6:207969/119‑1 (MQ=255)                                                                                  aaTAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCCGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCaga                                                                                            >  5:207969/1‑119 (MQ=255)                                                                                           gATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCaaa                                                      <  3:192364/148‑1 (MQ=255)                                                                                                 ggTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGTAACCGACCTGCGCTGACGCTTCATTTGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCAGc                                               <  2:296598/149‑1 (MQ=255)                                                                                                 ggTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCAg                                                <  5:36948/148‑1 (MQ=255)                                                                                                        gTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCAGCGTTCTGc                                        <  3:343220/149‑1 (MQ=255)                                                                                                           aTGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCAGCGTTCTGCGAt                                     <  4:293475/149‑1 (MQ=255)                                                                                                           aTGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCAGCGTTCTGCGAt                                     >  8:342634/1‑149 (MQ=255)                                                                                                              cAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCAGCGTTCTGCGATgg                                   <  3:295853/148‑1 (MQ=255)                                                                                                                      ggtGAACATACAGTTAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAAc                          <  2:243735/147‑1 (MQ=255)                                                                                                                           aCATACAGTTAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCAt                       >  7:284396/1‑147 (MQ=255)                                                                                                                            cATACAGTTAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAg                    >  2:340410/1‑149 (MQ=255)                                                                                                                             aTACAGTTAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCa                                                 >  6:83090/1‑119 (MQ=255)                                                                                                                             aTACAGTTAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCa                                                 <  5:83090/119‑1 (MQ=255)                                                                                                                               aCAGTTAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGc                   >  8:328507/1‑147 (MQ=255)                                                                                                                                   ttAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCg                                                                                                  <  5:203829/64‑1 (MQ=255)                                                                                                                                   ttAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCg                                                                                                  >  6:203829/1‑64 (MQ=255)                                                                                                                                        aaTGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATa                                                                                                             >  4:352195/1‑48 (MQ=255)                                                                                                                                        aaTGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATa                                                                                                             <  3:352195/48‑1 (MQ=255)                                                                                                                                        aaTGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACt          >  6:321572/1‑147 (MQ=255)                                                                                                                                        aaTGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCa        <  7:342634/149‑1 (MQ=255)                                                                                                                                              aCCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCAc  <  7:205220/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                              TTTGGTTTCATCGTAAGCATCTTCAGAACCAAAAAGTTCTTTCGCCACTTCACGTTGCAGTTTCAATGGCAAGCCGGAACCAATAAAGTTGATATGGGTATGCGTCATGCAGTCACGGTTGAACATACAGTTAACAATGGAACCGACCTGCGCTGACGCTTCATTGGCATGGTGAACCGGATAGCCAAACGGCGACCAGAGTTTATTTTTCGCGTAGCCCCAGTACTCTTCACCCAAATTCCAGCGTTCTGCGATGGCATATGAACCATCAGCCAGGTGACTCAGCTCAC  >  NC_000913/1752244‑1752533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑