Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,512,045 T→C 7.1% Q221R (CAG→CGG)  mntH ← manganese/divalent cation transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,512,0450TC 7.1% 165.9 / 3.9 56Q221R (CAG→CGG) mntHmanganese/divalent cation transporter
Reads supporting (aligned to +/- strand):  ref base T (19/33);  new base C (2/2);  total (21/35)
Fisher's exact test for biased strand distribution p-value = 6.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.39e-01

GCAACACCAGTATGACCGGAAAAGTGGAACGCCGCCGCAGCTGTAGCCATCATCGCCAGATTGACAAAACCGGCAATAGTCATGGCGATAGCCACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCTGAGTGAGCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGCCCCTAACACGCCTGCTGCCAGGAAGACCGCTTCCGAAGTAGGTAAACTCGGGATCACCATTCCTTTACCCAGCTGCGCCAGGTTAGGCTGGGAGAAA  >  NC_000913/2511898‑2512188
                                                                                                                                                   |                                                                                                                                               
gCAACCCCAGTATGACCGGAAAAGTGGCACGCCGCCGCAGCTGTAGCCATCATCTCCACATTGACAAAACCGGCAATAGTCATGGCGATAGCCACAACCGATTTGGTGGCGGAATAACGTTGTTTCCGCGAACCGCCATGTAAATGCt                                                                                                                                                 >  4:290381/1‑148 (MQ=255)
  aaCACCAGTATGACCGGAAAAGTGGAACGCCGCCGCAGCTGTAGCCATCATCGCCAGATTGACAAAACCGGCAATAGTCATGGCGATAGCCACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCtgag                                                                                                                                              >  7:245841/1‑149 (MQ=255)
     acCAGTATGACCGGAAAAGTGGAACGCCGCCGCAGCTGTAGCCATCATCGCCAGATTGACAAAACCGGCAATAGTCATGGCGATAGCCACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGCCCCGCCATGTAAATGCtgagt                                                                                                                                             >  6:1073/1‑147 (MQ=255)
     acCAGTATGACCGGAAAAGTGGAACGCCGCCGCAGCTGTAGCCATCATCGCCAGATTGACAAAACCGGCAATAGTCATGGCGATAGCCACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCtgagt                                                                                                                                             >  7:247390/1‑147 (MQ=255)
     acCAGTATGACCGGAAAAGTGGAACGCCGCCGCAGCTGTAGCCATCATCGCCAGATTGACAAAACCGGCAATAGTCATGGCGATAGCCACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCt                                                                                                                                                 >  8:89287/1‑143 (MQ=255)
     acCAGTATGACCGGAAAAGTGGAACGCCGCCGCAGCTGTAGCCATCATCGCCAGATTGACAAAACCGGCAATAGTCATGGCGATAGCCACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCt                                                                                                                                                 <  7:89287/143‑1 (MQ=255)
       cAGTATGACCGGAAAAGTGGAACGCCGCCGCAGCTGTAGCCATCATCGCCAGATTGACAAACCCGGCAATAGTCATGGCGATAGCCACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCTGAGTGAGc                                                                                                                                         <  6:214013/149‑1 (MQ=255)
       cAGTATGACCGGAAAAGTGGAACGCCGCCGCAGCTGTAGCCATCATCGCCAGATTGACAAAACCGGCAATAGTCATGGCGATAGCCACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCACGTAAATGCTGAGTGAGc                                                                                                                                         >  3:172186/1‑149 (MQ=255)
         gTATGACCGGAAAAGTGGAACGCCGCCGCAGCTGTAGCCATCATCGCCAGATTGACAAAACCGGCAATAGTCATGGCGATAGCCACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCTGAGTGAGCga                                                                                                                                       <  3:309646/149‑1 (MQ=255)
          tATGACCGGAAAAGTGGAACGCCGCCGCAGCTGTAGCCATCATCGCCAGATTGACAAAACCGGCAATAGTCATGGCGATAGCCACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCTGAGTGAGCgag                                                                                                                                      <  1:193805/149‑1 (MQ=255)
          tATGACCGGAAAAGTGGAACGCCGCCGCAGCTGTAGCCATCATCGCCAGATTGACAAAACCGGCAATAGTCATGGCGATAGCCACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCTGAGTGAGCgag                                                                                                                                      <  2:274454/149‑1 (MQ=255)
            tGACCGGAAAAGTGGAACGCCGCCGCAGCTGTAGCCATCATCGCCAGATTGACAAAACCGGCAATAGTCATGGCGATAGCCACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCTGAGTGAGCgagga                                                                                                                                    <  1:326799/149‑1 (MQ=255)
              aCCGGAAAAGTGGATCGCCGCCGCAGCTGTAGCCATCATCGCCAGATTGACAAAACCGGCAATAGTCATGGCGATAGCCACATCCCATTTGGTGGCGTAATAACGTTGTTGACGCGAACCGCCATGTAAATGCTGAGTGAGCGAGGAGt                                                                                                                                  <  1:354455/149‑1 (MQ=255)
              aCCGGAAAAGTGGAACGCCGCCGCAGCTGTAGCCATCATCGCCAGATTGACAAAACCGGCAATAGTCATGGCGATAGCCACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCTGAGTGAGCGAGGAGt                                                                                                                                  <  1:293084/149‑1 (MQ=255)
                cGGAAAAGTGGAACGCCGCCGCAGCTGTAGCCATCATCGCCAGATTGACAAAACCGGCAATAGTCATGGCGATAGCCACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCCGAGTGAGCGAGGAGTg                                                                                                                                 >  7:253918/1‑148 (MQ=255)
                  gAAAAGTGGAACGCCGCCGCAGCTGTAGCCATCATCGCCAGATTGACAAAACCGGCAATAGTCATGGCGATAGCCACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCtgagtga                                                                                                                                           >  2:59456/1‑136 (MQ=255)
                  gAAAAGTGGAACGCCGCCGCAGCTGTAGCCATCATCGCCAGATTGACAAAACCGGCAATAGTCATGGCGATAGCCACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCtgagtga                                                                                                                                           <  1:59456/136‑1 (MQ=255)
                  gAAAAGTGGAACGCCGCCGCAGCTGTAGCCATCATCGCCAGATTGACAAAACCGGCAATAGTCATGGCGATAGCCACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCTGAGTGAGCGAGGAGTGCaa                                                                                                                              <  8:227530/149‑1 (MQ=255)
                   aaaaGTGGACCGCCGCCGCAGCTGTAGCCATCATCGCCAGATTGACAAAACCGGCAATAGTCATGGCGATAGCCACATCCCATTTGGTGGCGGAATAACGTTGTGGACGCGAACCGCCATGTAAATGCTGAGGGAGCGAGGAGTGCaaa                                                                                                                             <  6:338097/149‑1 (MQ=255)
                       gTGGAACGCCGCCGCAGCTGTAGCCATCATCGCCAGATTGACAAAACCGGCAATAGTCATGGCGATAGCCACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCTGAGTGAGCGAGGAGTGCaaataaa                                                                                                                         <  5:3762/149‑1 (MQ=255)
                       gTGGAACGCCGCCGCAGCTGTAGCCATCATCGCCAGATTGACAAAACCGGCAATAGTCATGGCGATAGCCACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCTGAGTGAGCGAGGAGTGCaaataaa                                                                                                                         <  1:137706/149‑1 (MQ=255)
                        tGGAACGCCGCCGCAGCTGTAGCCATCATCGCCAGATTGACAAAACCGGCAATAGTCATGGCGATAGCCACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCTGAGTGAGCGAGGAGTGCaaataaat                                                                                                                        <  8:247390/149‑1 (MQ=255)
                        tGGAACGCCGCCGCAGCTGTAGCCATCATCGCCAGATTGACAAAACCGGCAATAGTCATGGCGATAGCCACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCTGAGTGAGCGAGGAGTGCaaataaat                                                                                                                        <  3:356038/149‑1 (MQ=255)
                        tGGAACGCCGCCGCAGCTGTAGCCATCATCGCCAGATTGACAAAACCGGCAATAGTCATGGCGATAGCCACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCTGAGTGAGCGAGGAGTGCaaataaat                                                                                                                        <  8:245841/149‑1 (MQ=255)
                           aaCGCCGCCGCAGCTGTAGCCATCATCGCCAGATTGACAAAACCGGCAATAGTCATGGCGATAGCCACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCCGAGTGAGCGAGGAGTGCAAATAAATcac                                                                                                                     <  8:253918/149‑1 (MQ=255)
                                cgccgcAGCTGTAGCCATCATCGCCAGATTGACAAAACCGGCAATAGTCATGGCGATAGCCACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCTGAGTGAGCGAGGAGTGCAAATAAATCACATGCg                                                                                                                >  2:52584/1‑149 (MQ=255)
                                    gcAGCTGTAGCCATCATCGCCAGATTGACAAAACCGGCAATAGTCATGGCGATAGCCCCATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCTGAGTGAGCGAGGAGTGCAAATAAATCACATGCgg                                                                                                               >  8:339331/1‑146 (MQ=255)
                                               catcatCGCCAGATTGACAAAACCGGCAATAGTCATGGCGATAGCCACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCTGAGTGAGCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGccc                                                                                                 <  8:305240/149‑1 (MQ=255)
                                                   atcGCCAGATTGACAAAACCGGCAATAGTCATGGCGATAGCCACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCTGAGTGAGCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGCACCTaa                                                                                             >  4:195664/1‑149 (MQ=255)
                                                        cAGATTGACAAAACCGGCAATAGTCATGGCGATAGCCACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCTGAGTGAGCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGCCCCTAACACGc                                                                                        >  1:25699/1‑149 (MQ=255)
                                                               aCAAAACCGGCAATAGTCATGGCGATAGCCACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCTGAGTGAGCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGCCCCTAACCCGCCtgctgc                                                                                 >  2:100712/1‑149 (MQ=255)
                                                                   aaCCGGCAATAGTCATGGCGATAGCCACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCTGAGTGAGCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGCCCCTAACACGCCTGCTGCCAgg                                                                             <  4:163168/149‑1 (MQ=255)
                                                                           aTAGTCATGGCGATAGCCACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCTGAGTGAGCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGCCCCTAACACGCCTGCTGCCAGGAAGACCGc                                                                     >  3:78286/1‑149 (MQ=255)
                                                                               tCATGGCGATAGCCACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCTGAGTGAGCGAGGAGTGCAAATAAATCACATGCGGCTTAATCGTCGCCCCTAACACGCCTGCTGCCAGGAAGACCGCTTcc                                                                 >  2:228318/1‑149 (MQ=255)
                                                                                 aTGGCGATAGCCACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCtga                                                                                                                                               <  8:217473/69‑1 (MQ=255)
                                                                                 aTGGCGATAGCCACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCtga                                                                                                                                               >  7:217473/1‑69 (MQ=255)
                                                                                 aTGGCGATAGCCACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCTGAGTGAGCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGCCCCTAACACGCCTGCTGCCAGGAAGACCGCTTCCGa                                                               <  2:25699/149‑1 (MQ=255)
                                                                                  tGGCGATAGCCACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCTGAGTGAGCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGCCCCTAACACGCCTGCTGCCAGGAAGACCGCTTCCg                                                                >  8:75780/1‑147 (MQ=255)
                                                                                           ccACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCTGAGTGAGCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGCCCCTAACACGCCTGCTGCCAGGAAGACCGCTTCCGAAGTAGGTaaa                                                     >  5:223747/1‑149 (MQ=255)
                                                                                           ccACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCCGAGTGAGCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGCCCCTAACACGCCTGCTGCCAGGaa                                                                           <  2:120862/127‑1 (MQ=255)
                                                                                           ccACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCCGAGTGAGCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGCCCCTAACACGCCTGCTGCCAGGaa                                                                           >  1:120862/1‑127 (MQ=255)
                                                                                            cacaTCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCTGAGTGAGCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGCCCCTAACACGCCTGCTGCCAGGAAGACCGCTTCCGAAGTAGGTAAAc                                                    <  3:195664/149‑1 (MQ=255)
                                                                                                tCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCTGAGTGAGCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGCCCCTAACACGCCTGCTGCCAGGAAGACCGCTTCCGAAGTAGGTAAACTCgg                                                <  5:90620/149‑1 (MQ=255)
                                                                                                tCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCTGAGTGAGCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGCCCCTAACACGCCTGCTGCCAGGAAGACCGCTTCCGAAGTAGGTAAACTCgg                                                >  4:21318/1‑149 (MQ=255)
                                                                                                  ccATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCTGAGTGAGCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGCCCCTAACACGCCTGCTGCCAGGAAGACCGCTTCCGAAGTAGGTAAACTCGGGa                                              <  3:161743/149‑1 (MQ=255)
                                                                                                   cATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCTGAGTGAGCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGCCCCTAACACGCCTGCTGCCAGGAAGACCGCTTCCGAAGTAGGTAAACTCGGGAt                                             <  8:327524/149‑1 (MQ=255)
                                                                                                          tggCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCTGAGTGAGCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGCCCCTAACACGCCTGCTGCCAGGAAGACCGCTTCCGAAGTAGGTAAACTCGGGATCACCAtt                                      >  2:206875/1‑149 (MQ=255)
                                                                                                            gCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCTGAGTGAGCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGCCCCTAACACGCCTGCTGCCAGGAAGACCGCTTCCGAAGTAGGTAAACTCGGGATCACCATTcc                                    <  3:21318/149‑1 (MQ=255)
                                                                                                                  tAACGTTGTTGACGCGAACCGCCATGTAAATGCTGAGTGAGCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGCCCCTAACACGCCTGCTGCCAGGAAGACCGCTTCCGAAGTAGGTAAACTCGGGATCACCATTCCTTTAcc                              <  7:339331/149‑1 (MQ=255)
                                                                                                                    aCGTTGTTGACGCGAACCGCCATGTAAATGCTGAGTGAGCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGCCCCTAACACGCCTGCTGCCAGGAAGACCGCTTCCGAAGTAGGTAAACTCGGGATCACCATTCCTTTAccc                             <  6:223747/148‑1 (MQ=255)
                                                                                                                      gttgttGACGCGAACCGCCATGTAAATGCTGAGTGAGCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGCCCCTAACACGCCTGCTGCCAGGAAGACCGCTTCCGAAGTAGGTAAACTCGGGATCa                                           >  5:328104/1‑132 (MQ=255)
                                                                                                                      gttgttGACGCGAACCGCCATGTAAATGCTGAGTGAGCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGCCCCTAACACGCCTGCTGCCAGGAAGACCGCTTCCGAAGTAGGTAAACTCGGGATCa                                           <  6:328104/132‑1 (MQ=255)
                                                                                                                      gttgttGACGCGAACCGCCATGTAAATGCTGAGTGAGCGAGGAGTGCAAATAAATCACAT‑CGGCATAATCGTCGCCCCTAACACGCCTGCTGCCAGGAAGACCGCTTCCGAAGTAGGTAAACTCGGGATCACCATTCCTTTACCCAGCt                         >  1:26344/1‑149 (MQ=255)
                                                                                                                                cgAACCGCCATGTAAATGCTGAGTGAGCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGCCCCTAACACGCCTGCTGCCAGGAAGACCGCTTCCGAAGTAGGTAAACTCGGGATCACCATTCCTTTACCCAGCTGCGCCAGGt                <  2:125244/149‑1 (MQ=255)
                                                                                                                                cgAACCGCCATGTAAATGCTGAGGGAGCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGCCCCTAACACGCCTGCTGCCAGGAAGACCGCTTCCGAAGTAGGGAAACTCGGGATCACGATTCCTTTACCCAGCTGCGCCAGGt                <  3:290381/149‑1 (MQ=255)
                                                                                                                                    ccGCCATGTAAATGCTGAGTGAGCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGCCCCTAACACGCCTGCTGCCAGGAAGACCGCTTCCGAAGTAGGTAAACTCGGGATCACCATTCCTTTACCCAGCTGCGCCAGGTTAgg            >  7:238359/1‑149 (MQ=255)
                                                                                                                                     cGCCATGTAAATGCTGAGTGAGCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGCCCCTAACACGCCTGCTGCCAGGAAGACCGCTTCCGAAGTAGGTAAACTCGGGATCACCATTCCTTTACCCAGCTGCGCCAGGTTAGGc           <  1:206875/149‑1 (MQ=255)
                                                                                                                                       ccATGTAAATGCTGAGTGAGCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGCCCCTAACACGCCTGCTGCCAGGAAGACCGCATCCGAAGTAGGTAACCTCTGGATCACAATTCCTTTACCCAGCTGCGCACGGTTAGGCTg         >  5:241689/1‑149 (MQ=255)
                                                                                                                                       ccATGTAAATGCTGAGTGAGCGAGGAGTGCAAATAAATCACAT‑CGGCATAATCGTCGCCCCTAACACGCCTGCTGCCAGGAAGACCGCTTCCGAAGTAGGTAAACTCGGGATCACCATTCCTTTACCCAGCTGCGCCAGGTTAGGCTgg        <  2:26344/149‑1 (MQ=255)
                                                                                                                                        cacgTAAATGCTGAGTGAGCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGCCCCTAACACGCCTGCTGCCAGGAAGACCGCTTCCGAAGTAGGTAAACTCGGGATCACCATTCCTTTACCCAGCTGCGCCAGGTTAGGCTgg        <  4:172186/146‑1 (MQ=255)
                                                                                                                                         aTGTAAATGCTGAGTGAGCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGCCCCTAACACGCCTGCTGCCAGGAAGACCGCTTCCGAAGTAg                                                          >  3:47703/1‑98 (MQ=255)
                                                                                                                                         aTGTAAATGCTGAGTGAGCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGCCCCTAACACGCCTGCTGCCAGGAAGACCGCTTCCGAAGTAg                                                          <  4:47703/98‑1 (MQ=255)
                                                                                                                                             aaaTGCTGAGTGAGCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGCCCCTAACACGCCTGCTGCCAGGAAGACCGCTTCCGAAGTAGGTAAACTCGGGATCACCATTCCTTTACCCAGCTGCGCCAGGTTAGGCTgg        >  7:227587/1‑144 (MQ=255)
                                                                                                                                             aaaTGCTGAGTGAGCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGCCCCTAACACGCCTGCTGCCAGGAAGACCGCTTCCGAAGTAGGTAAACTCGGGATCACCATTCCTTTACCCAGCTGCGCCAGGTTAGGCTgg        <  8:227587/144‑1 (MQ=255)
                                                                                                                                             aaaTGCTGAGTGAGCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGCCCCTAACACGCCTGCTGCCAGGAAGACCGCTTCCGAAGTAGGTAAACTCGGGATCACCATTCCTTTACCCAGCTGCGCCAGGTTAGGCTGGgaga    >  1:300650/1‑148 (MQ=255)
                                                                                                                                              aaTGCTGAGTGAGCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGCCCCTAACACGCCTGCTGCCAGGAAGACCGCTTCCGAAGTAGGTAAACTCGGGATCACCATTCCTTTACCCAGCTGCGCCAGGTTAGGCTGGGAGaaa  <  6:272239/149‑1 (MQ=255)
                                                                                                                                                   tgagtgagCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGCCCCTAACACGCCTGCTGCCAGGaa                                                                           >  1:698/1‑71 (MQ=255)
                                                                                                                                                   tgagtgagCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGCCCCTAACACGCCTGCTGCCAGGaa                                                                           <  2:698/71‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
GCAACACCAGTATGACCGGAAAAGTGGAACGCCGCCGCAGCTGTAGCCATCATCGCCAGATTGACAAAACCGGCAATAGTCATGGCGATAGCCACATCCCATTTGGTGGCGGAATAACGTTGTTGACGCGAACCGCCATGTAAATGCTGAGTGAGCGAGGAGTGCAAATAAATCACATGCGGCATAATCGTCGCCCCTAACACGCCTGCTGCCAGGAAGACCGCTTCCGAAGTAGGTAAACTCGGGATCACCATTCCTTTACCCAGCTGCGCCAGGTTAGGCTGGGAGAAA  >  NC_000913/2511898‑2512188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑