Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,840,650 C→A 7.3% P376Q (CCG→CAG)  ascF → cellobiose/arbutin/salicin‑specific PTS enzymes, IIB and IC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,840,6500CA 7.3% 200.2 / 2.9 62P376Q (CCG→CAG) ascFcellobiose/arbutin/salicin‑specific PTS enzymes, IIB and IC components
Reads supporting (aligned to +/- strand):  ref base C (28/29);  new base A (3/2);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGATGGTCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTGGGCGGTTCATCACTGGCAGTGGCGTGGAAAACGAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTGCCAGCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAGTTCTT  >  NC_000913/2840506‑2840797
                                                                                                                                                |                                                                                                                                                   
ggATGGTCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTGGGCGGTTCATCACTGGCAGTGGCGTGGAAAACGAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCg                                                                                                                                                 <  7:315127/149‑1 (MQ=255)
ggATGGTCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTGGGCGGTTCATCACTGGCAGTGGCGTGGAAAACGAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCg                                                                                                                                                 <  7:236171/149‑1 (MQ=255)
     gTCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTGGGCGGTTCATCACTGGCAGTGGCGTGGAAAACGAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTtat                                                                                                                                             <  5:311333/148‑1 (MQ=255)
      tCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTGGGCGGTTCATCACTGGCAGTGGTGTGGAAAACGAAAACCCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTatat                                                                                                                                           >  3:15255/1‑149 (MQ=255)
      tCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTGGGCGGTTCATCACTGGCAGTGGCGTGGAAAACGAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGtt                                                                                                                                               >  1:226924/1‑145 (MQ=255)
      tCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTGGGCGGTTCATCACTGGCAGTGGCGTGGAAAACGAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGtt                                                                                                                                               <  2:226924/145‑1 (MQ=255)
        aTGCCGTCAGAGATCGGCGCTAACCTGTCGCTGGGCGGTTCATCACTGGCAGTGGCGTGGAAAACGAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATAtg                                                                                                                                          <  5:48445/148‑1 (MQ=255)
          gCCGTCAGAGATCGGCGCTAACCTGTCGCTGGGCGGTTCATCACTGGCAGTGGCGTGGAAAACGAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCg                                                                                                                                                    <  4:159997/136‑1 (MQ=255)
          gCCGTCAGAGATCGGCGCTAACCTGTCGCTGGGCGGTTCATCACTGGCAGTGGCGTGGAAAACGAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCg                                                                                                                                                    >  3:159997/1‑136 (MQ=255)
                agagATCGGCGCTAACCTGTCGCTGGGCGGTTCATCACTGGCAGTGGCGTGGAAAACGAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACATGCGTTATATGTCGTGGCGa                                                                                                                                 >  4:214423/1‑149 (MQ=255)
                       ggCGCTAACCTGTCGCTGGGCGGTTCATCACTGGCAGTGGCGTGGAAAACGAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCt                                                                                                                          >  1:336846/1‑149 (MQ=255)
                              aCCTGTCGCTGGGCGGTTCATCACTGGCAGTGGCGTGGAAAACGAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAAc                                                                                                                     <  6:18767/147‑1 (MQ=255)
                                cTGTCGCTGGGCGGTTCATCACTGGCAGTGGCGTGGAAAACGAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTcc                                                                                                                 >  3:165773/1‑149 (MQ=255)
                                   tCGCTGGGCGGTTCATCACTGGCAGTGGCGTGGAAAACGAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCAATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGc                                                                                                               <  2:152255/148‑1 (MQ=255)
                                     gCTGGGCGGTTCATCACTGGCAGTGGCGTGGAAAACGAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTa                                                                                                            >  1:153181/1‑149 (MQ=255)
                                         ggCGGTTCATCACTGGCAGTGGCGTGGAAAACGAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATc                                                                                                          <  5:158200/147‑1 (MQ=255)
                                         ggCGGTTCATCACTGGCAGTGGCGTGGAAAACGAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGc                                                                                                        <  5:126794/149‑1 (MQ=255)
                                              ttCATCACTGGCAGTGGCGTGGAAAACGAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGc                                                                                                        <  2:168392/144‑1 (MQ=255)
                                              ttCATCACTGGCAGTGGCGTGGAAAACGAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGc                                                                                                        >  1:168392/1‑144 (MQ=255)
                                                 atcaCTGGCAGTGGCGTGGAAAACGAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGAATGAAACGTCCGCTTATCGCCAGTCtt                                                                                                 <  2:153181/148‑1 (MQ=255)
                                                 atcaCTGGCAGTGGCGTGGAAAACGAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACAGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTa                                                                                                >  3:129281/1‑149 (MQ=255)
                                                 atcaCTGGCAGTGGCGTGGAAAACGAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACAGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTa                                                                                                >  3:213556/1‑149 (MQ=255)
                                                  tcaCTGGCAGTGGCGTGGAAAACGAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTAt                                                                                               <  8:159985/149‑1 (MQ=255)
                                                  tcaCTGGCAGTGGCGTGGAAAACGAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTAt                                                                                               >  3:158912/1‑149 (MQ=255)
                                                    aCTGGCAGTGGCGTGGAAAACGAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATc                                                                                              <  5:125621/148‑1 (MQ=255)
                                                      tGGCAGTGGCGTGGAAAACGAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGc                                                                                           >  1:276319/1‑149 (MQ=255)
                                                        gCAGTGGCGTGGAAAACGAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCgc                                                                                         >  7:249542/1‑148 (MQ=255)
                                                          aGTGGCGTGGAAAACGAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGtt                                                                                       <  4:238292/149‑1 (MQ=255)
                                                          aGTGGCGTGGAAAACGAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGtt                                                                                       >  2:36707/1‑149 (MQ=255)
                                                            tGGCGTGGAAAACGAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATAtggcgtgg                                                                                                                                    >  8:18828/1‑102 (MQ=255)
                                                              gCGTGGAAAACGAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTa                                                                                    <  2:290336/148‑1 (MQ=255)
                                                              gCGTGGAAAAAGAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTAGGGCGGGGATTTCCGAACCGGCGGTAAATGGCGTGGCGATCCGCATGAAACGTCCGCTTATCGCCAGTCTGATCAGCGTGTTTAt                                                                                   >  7:309133/1‑149 (MQ=255)
                                                                 tGGAAAACGAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTg                                                                                <  2:336846/149‑1 (MQ=255)
                                                                     aaaCGAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTgcggc                                                                            >  4:29136/1‑149 (MQ=255)
                                                                      aaCGAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTgcggcg                                                                           >  8:188839/1‑149 (MQ=255)
                                                                         gAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTGCGGCGCgg                                                                        >  7:78449/1‑149 (MQ=255)
                                                                           aaaaCCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTGCGGCTCGGtt                                                                      >  6:48104/1‑149 (MQ=255)
                                                                           aaaaCCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTGCGGCGCGGtt                                                                      <  7:188839/149‑1 (MQ=255)
                                                                             aaCCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTGCGGCGCGGTTGc                                                                    <  8:249542/149‑1 (MQ=255)
                                                                              aCCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTGCGGCGCGGTTGcc                                                                   <  5:73818/149‑1 (MQ=255)
                                                                               cccGGAACTGCGTGAGACGGCGCTGGCTGCGGCGGCATCAGCCTTTATGGCGGTGTTTTCCGAACCGGCGTTATATGGCGGGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTGCGGCGCGGTTGCCg                                                                  <  4:246553/149‑1 (MQ=255)
                                                                               cccGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTGCGGCGCGGTTGCCg                                                                  >  1:147681/1‑149 (MQ=255)
                                                                                     aCTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATGTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTGATCGCCAGTCTTATCAGCGGTTTTATTTGCGGCGCGGTTGCCGGTATgg                                                            <  4:15255/149‑1 (MQ=255)
                                                                                      cTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTGCGGCGCGGTTGCCGGTATGGc                                                           <  3:29136/149‑1 (MQ=255)
                                                                                         cGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTGCGGCGCGGTTGCCGGTATGGCgg                                                         <  3:214423/148‑1 (MQ=255)
                                                                                               aCGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACAGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTGCGGCGCTGTTGCCGGTATGGCGGGGCTTgc                                                  <  4:129281/149‑1 (MQ=255)
                                                                                                cGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACAGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTgc                                                  <  4:213556/148‑1 (MQ=255)
                                                                                                   cgcTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTgccagc                                              <  2:147681/149‑1 (MQ=255)
                                                                                                     ctggctgCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTgccagcca                                            >  4:346378/1‑149 (MQ=255)
                                                                                                     ctggctgCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTgccagcca                                            <  4:165773/149‑1 (MQ=255)
                                                                                                               cggcATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTGCCAGCCACTCAATGGCa                                  <  2:276319/149‑1 (MQ=255)
                                                                                                                   aTCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTGCCAGCCACTCAATGGCAgcgc                              >  3:212987/1‑149 (MQ=255)
                                                                                                                       gCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTgcc                                                 <  2:349541/126‑1 (MQ=255)
                                                                                                                       gCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTgcc                                                 >  1:349541/1‑126 (MQ=255)
                                                                                                                        ccATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAACCGTCCGCTTATCGCCAGTCGTATCAGCGGTTTTAGTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTGCCAGCCACTCAATGGCAGCGCCgggg                         >  7:385365/1‑149 (MQ=255)
                                                                                                                         cATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTGCCAGCCACTCAATGGCAGCGCCGGGGc                        >  7:179344/1‑149 (MQ=255)
                                                                                                                         cATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTGCCAGCCACTCAATGGCAGCGCCGGGGc                        <  4:158912/149‑1 (MQ=255)
                                                                                                                               ggCGGGGATTTCCGAAACGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTGCCAGCCACTCAATGGCAGCGCCGGGGCTATTTa                  >  7:383946/1‑149 (MQ=255)
                                                                                                                                   gggATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTGCCAGCCACTCAATGGCAGCGCCGGGGCTATTTACCAg              >  8:124981/1‑149 (MQ=255)
                                                                                                                                        ttCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTGCCAGCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTg          >  1:348604/1‑148 (MQ=255)
                                                                                                                                            gAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGACAGTCTTATCAGCGGTTTTATTTGCGGCGCGGTTGCCGGTATGCCTGCGCTTGCAAGCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCTTGCAGt      >  8:385735/1‑148 (MQ=255)
                                                                                                                                             aaCCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTGCCAGCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAGttc    >  1:30324/1‑149 (MQ=255)
                                                                                                                                               ccGGCGTTATATGGCGTGGCGATCCGCCTGCAACGGCCGCTTATCGCCAGTCTCATCAGCTGTTTTATTTGCGGCGCGGTTGCCGGTATGGCGGTGCGTGCCAGCCCCTCACTGGCAGCGCCGGGGCTATTTACCAGCGTGCAGttctt  <  8:309133/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
GGATGGTCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTGGGCGGTTCATCACTGGCAGTGGCGTGGAAAACGAAAAACCCGGAACTGCGTCAGACGGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTTCCGAACCGGCGTTATATGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTGCGGCGCGGTTGCCGGTATGGCGGGGCTTGCCAGCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAGTTCTT  >  NC_000913/2840506‑2840797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑