Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,945,020 C→T 5.1% intergenic (+35/+16) csdE → / ← tcdA CsdA‑binding activator; Fe‑S protein/tRNA threonylcarbamoyladenosine dehydratase; sulfur acceptor for CsdA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,945,0200CT 5.1% 253.5 / 2.7 78intergenic (+35/+16)csdE/tcdACsdA‑binding activator; Fe‑S protein/tRNA threonylcarbamoyladenosine dehydratase; sulfur acceptor for CsdA
Reads supporting (aligned to +/- strand):  ref base C (42/32);  new base T (2/2);  total (44/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01

CCACTGGCATTGTTTGATGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGCACAATCCATCCGCTTCGGCCCTTCTG  >  NC_000913/2944875‑2945166
                                                                                                                                                 |                                                                                                                                                  
ccacTGGCATTGTTTGATGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATc                                                                                                                                                 >  3:152975/1‑149 (MQ=255)
      gCATTGTTTGATGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCt                                                                                                                                           >  1:315049/1‑149 (MQ=255)
           gTTTGATGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGAtt                                                                                                                                       <  2:292820/148‑1 (MQ=255)
            tttGATGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCAGACCTGATTGt                                                                                                                                     >  4:297706/1‑149 (MQ=255)
              tgatgaGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGtt                                                                                                                                    <  3:1617/148‑1 (MQ=255)
                   aGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAAcc                                                                                                                               <  3:285386/148‑1 (MQ=255)
                     cTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCt                                                                                                                             >  1:112555/1‑148 (MQ=255)
                      tGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGa                                                                                                                           >  7:61271/1‑149 (MQ=255)
                                  cgcAGCTTAGCGCCTCACGCAGCCAGTGGTTAAATGCTTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTc                                                                                                               <  3:297706/149‑1 (MQ=255)
                                    cAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGc                                                                                                             >  8:44679/1‑149 (MQ=255)
                                       cTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATGAGACGCGAACGTCTTATCCGAccc                                                                                                                                           <  2:375403/116‑2 (MQ=38)
                                       cTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATGAGACGCGAACGTCTTATCCGAccc                                                                                                                                           >  1:375403/1‑115 (MQ=38)
                                        ttAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGa                                                                                                                                         >  3:155089/1‑117 (MQ=255)
                                        ttAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGa                                                                                                                                         <  4:155089/117‑1 (MQ=255)
                                          aGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGTcatcat                                                                                                       >  3:62562/1‑149 (MQ=255)
                                          aGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCcatcat                                                                                                       >  1:330729/1‑149 (MQ=255)
                                           gcgcCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCatcatc                                                                                                      <  7:44679/149‑1 (MQ=255)
                                              ccTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATACGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCAtcttc                                                                                                   >  7:1200/1‑149 (MQ=255)
                                                tCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTc                                                                                                               >  2:155188/1‑135 (MQ=255)
                                                tCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTc                                                                                                               <  1:155188/135‑1 (MQ=255)
                                                         cAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCAt                                                                                         >  7:199524/1‑148 (MQ=255)
                                                          aGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCAtcttc                                                                                                   <  1:247662/137‑1 (MQ=255)
                                                          aGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCAtcttc                                                                                                   >  2:247662/1‑137 (MQ=255)
                                                          aGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCa                                                                                               <  1:11682/141‑1 (MQ=255)
                                                          aGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCa                                                                                               >  2:11682/1‑141 (MQ=255)
                                                          aGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATga                                                                                       <  4:152975/149‑1 (MQ=255)
                                                          aGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATga                                                                                       <  1:235790/149‑1 (MQ=255)
                                                          aGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATga                                                                                       <  2:112555/149‑1 (MQ=255)
                                                                  aaTGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCg                                                                                >  1:387062/1‑148 (MQ=255)
                                                                    tGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGcaa                                                                             <  8:339800/149‑1 (MQ=255)
                                                                     gCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGcaa                                                                             >  2:250248/1‑148 (MQ=255)
                                                                        ttAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACaaa                                                                         >  6:169070/1‑149 (MQ=255)
                                                                              gAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCaaa                                                                   <  2:315049/149‑1 (MQ=255)
                                                                              gAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCaaa                                                                   <  5:199736/149‑1 (MQ=255)
                                                                              gAGGCGATTATCGCTGCGACGAAGCAGGGTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGc                                                                               <  4:140439/137‑1 (MQ=255)
                                                                              gAGGCGATTATCGCTGCGACGAAGCAGGGTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGc                                                                               >  3:140439/1‑137 (MQ=255)
                                                                                 gCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCGTCTTCa                                                                                               >  8:39500/1‑118 (MQ=255)
                                                                                 gCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCCTCTTCa                                                                                               <  7:39500/118‑1 (MQ=255)
                                                                                   gATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACaaa                                                                         <  1:66669/138‑1 (MQ=255)
                                                                                   gATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACaaa                                                                         >  2:66669/1‑138 (MQ=255)
                                                                                   gATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTgg                                                              <  2:330729/149‑1 (MQ=255)
                                                                                    aTTATCTCTGCGACGAAGCAGGTTTAAGCAAAGAACAGGGTCGGATAAGACTTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCAATCATATTCTTCAGCGCATGAGAAACCGCAAAAAACCCAAAGGTGGc                                                             <  1:271084/149‑1 (MQ=255)
                                                                                    aTTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGc                                                             <  7:194475/149‑1 (MQ=255)
                                                                                     ttATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCg                                                            <  8:139049/149‑1 (MQ=255)
                                                                                               gacgaAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATga                                                                                       >  3:324527/1‑112 (MQ=255)
                                                                                               gacgaAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATga                                                                                       <  4:324527/112‑1 (MQ=255)
                                                                                               gacgaAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCg                                                  >  5:297934/1‑149 (MQ=255)
                                                                                                  gaAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTg                                               <  8:199524/149‑1 (MQ=255)
                                                                                                   aaGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCAtcttc                                                                                                   <  5:60244/96‑1 (MQ=255)
                                                                                                   aaGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCAtcttc                                                                                                   >  6:60244/1‑96 (MQ=255)
                                                                                                       aGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCcgcg                                          >  1:244339/1‑149 (MQ=255)
                                                                                                               gCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATc                                   >  4:346136/1‑148 (MQ=255)
                                                                                                               gCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATc                                   >  1:767/1‑148 (MQ=255)
                                                                                                                cacaGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAAcc                                                                                 <  1:256217/101‑1 (MQ=255)
                                                                                                                cacaGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAAcc                                                                                 >  2:256217/1‑101 (MQ=255)
                                                                                                                 acaGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATc                                   >  6:38165/1‑146 (MQ=255)
                                                                                                                   aGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGAt                              <  5:169070/149‑1 (MQ=255)
                                                                                                                   aGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGAt                              <  2:387062/149‑1 (MQ=255)
                                                                                                                     aaCCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATg                             >  2:171194/1‑148 (MQ=255)
                                                                                                                       tcaggTCGGATGAGACGCGAACGTCTTATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCa                                                                                               >  7:100474/4‑80 (MQ=21)
                                                                                                                       tcaggTCGGATGAGACGCGAACGTCTTATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCa                                                                                               <  8:100474/77‑1 (MQ=21)
                                                                                                                       ccGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGca                           >  6:349487/1‑148 (MQ=255)
                                                                                                                         gggTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGCACaa                        >  2:187425/1‑149 (MQ=255)
                                                                                                                          ggTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGCACAat                       >  1:239297/1‑149 (MQ=255)
                                                                                                                          ggTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGCACAat                       <  5:38165/149‑1 (MQ=255)
                                                                                                                               gATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGCACAatccatc                  >  8:102548/1‑149 (MQ=255)
                                                                                                                               gATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGCACAatccatc                  >  4:46990/1‑149 (MQ=255)
                                                                                                                                 tAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGCACAATCCATCCg                >  8:337851/1‑149 (MQ=255)
                                                                                                                                  aaGACGTTCGCGTCTCATACGACCTGATTGTTTAACCCTGCCGCGCCGCTTTCGCCATCATCTTGTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCCCCATCTTTGCCGCGCCAACTCCAGATGCACACTCCATCCGc               >  2:170510/1‑149 (MQ=255)
                                                                                                                                     aCGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGtt                                                <  7:368454/113‑1 (MQ=255)
                                                                                                                                     aCGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGtt                                                >  8:368454/1‑113 (MQ=255)
                                                                                                                                     aCGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGCACAATCCATCCGCTTc            >  7:190770/1‑149 (MQ=255)
                                                                                                                                     aCGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGCACAATCCATCCGCTTc            <  2:767/149‑1 (MQ=255)
                                                                                                                                       gTTCGCGTCTCATCCGACCTGATAGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAAACCCAACAAACCCAAAGGTGGCGGACACCATCGTTGCCGCGCCAAATCCAGATGCACAAACCATCCGCTTCgg          >  3:211885/1‑149 (MQ=255)
                                                                                                                                         tCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAACCCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGCACAATCCATCCGCTTCGGcc        >  1:315530/1‑149 (MQ=255)
                                                                                                                                           gcgTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGCACAATCCATCCGCTTCGGCCCt      >  4:139802/1‑149 (MQ=255)
                                                                                                                                           gcgTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGCACAATCCATCCGCTTCGGCCCt      >  5:126222/1‑149 (MQ=255)
                                                                                                                                            cgTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGCACAATCCATCCGCTTCGGCCCtt     <  3:346136/149‑1 (MQ=255)
                                                                                                                                               ctcATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGCACAATCCATCCGCTTCGGCCCTTCTg  >  1:127181/1‑149 (MQ=255)
                                                                                                                                               ctcATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGATGCCCCGCCAAATCCAGATGCACAATCCATCCGCTTCGCCCCTACTg  >  6:356013/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CCACTGGCATTGTTTGATGAGCTGGGATTACGTGCGCAGCTTAGCGCCTCACGCAGCCAGGGGTTAAATGCGTTAAGCGAGGCGATTATCGCTGCGACGAAGCAGGTTTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACCCTGACGCGCCGCTTTCGCCATCATCTTCTTCAGCGCATGAGAAACCGCAACAAACCCAAAGGTGGCGGTCACCATCGTTGCCGCGCCAAATCCAGATGCACAATCCATCCGCTTCGGCCCTTCTG  >  NC_000913/2944875‑2945166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: