Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 457,671 A→G E82E (GAA→GAG clpX → ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913457,6710AG99.2% 435.7 / NA 118E82E (GAA→GAGclpXATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (52/65);  minor base C (1/0);  total (53/65)
Fisher's exact test for biased strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

GTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAA  >  NC_000913/457525‑457816
                                                                                                                                                  |                                                                                                                                                 
gtgtATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCGGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCa                                                                                                                                                 <  7:117241/149‑1 (MQ=255)
 tgtATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAg                                                                                                                                                >  8:158775/1‑149 (MQ=255)
 tgtATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAg                                                                                                                                                >  5:328447/1‑149 (MQ=255)
   gatatCTGCGACGAAGGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGc                                                                                                                                              <  5:228444/148‑1 (MQ=255)
         tGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCa                                                                                                                                                 >  8:350894/1‑140 (MQ=255)
         tGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCa                                                                                                                                                 <  7:350894/140‑1 (MQ=255)
          gCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGaaaa                                                                                                                                         >  2:156240/1‑147 (MQ=255)
          gCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGaaaa                                                                                                                                         >  4:324413/1‑147 (MQ=255)
           cgacgaATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGaaaaaa                                                                                                                                       <  3:155171/148‑1 (MQ=255)
               gaATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCt                                                                                                                                  <  2:269791/149‑1 (MQ=255)
                aaTGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTg                                                                                                                                 <  3:324413/149‑1 (MQ=255)
                 aTGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTgg                                                                                                                                <  1:184543/149‑1 (MQ=255)
                 aTGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTgg                                                                                                                                <  6:328447/149‑1 (MQ=255)
                 aTGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTgg                                                                                                                                >  4:155726/1‑149 (MQ=255)
                    tgtTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACTCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCgg                                                                                                                             >  1:327187/1‑149 (MQ=255)
                    tgtTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCgg                                                                                                                             <  3:162487/149‑1 (MQ=255)
                     gtTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGt                                                                                                                            >  8:56715/1‑149 (MQ=255)
                      ttGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTc                                                                                                                           >  8:375470/1‑149 (MQ=255)
                             aTGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGc                                                                                                                                   <  5:159028/134‑1 (MQ=255)
                             aTGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGta                                                                                                                     <  2:415281/148‑1 (MQ=255)
                                 aaCGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTgg                                                                                                                                <  2:353490/133‑1 (MQ=255)
                                 aaCGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTgg                                                                                                                                >  1:353490/1‑133 (MQ=255)
                                 aaCGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACaa                                                                                                                >  3:290881/1‑149 (MQ=255)
                                       atcatTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGCACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCCCCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTa                                                                                                          >  5:83937/1‑149 (MQ=255)
                                       atcatTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTa                                                                                                          >  3:71614/1‑149 (MQ=255)
                                        tcatTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACaa                                                                                                                >  3:378194/1‑142 (MQ=255)
                                           ttCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGtat                                                                                                                    <  4:79229/135‑1 (MQ=255)
                                             cgcgAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAAc                                                                                                     <  7:95350/148‑1 (MQ=255)
                                             cgcgAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAAc                                                                                                     <  6:153131/148‑1 (MQ=255)
                                                 aaGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTcgcg                                                                                                                        >  2:265800/1‑125 (MQ=255)
                                                 aaGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTcgcg                                                                                                                        <  1:265800/125‑1 (MQ=255)
                                                   gagaTTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTgcg                                                                                              >  7:122364/1‑149 (MQ=255)
                                                      aTTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCCTGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCaa                                                                                           <  8:30346/149‑1 (MQ=255)
                                                         aaaGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACgg                                                                                        >  5:133899/1‑149 (MQ=255)
                                                          aagaagTTGCACCGCATCGTGAACGCAGTGCGCTACCGCCGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGc                                                                                       >  2:100053/1‑149 (MQ=255)
                                                          aagaagTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACgg                                                                                        <  8:432907/148‑1 (MQ=255)
                                                          aagaagTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGc                                                                                       <  2:25018/149‑1 (MQ=255)
                                                          aagaagTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGc                                                                                       <  1:156240/149‑1 (MQ=255)
                                                           tgaagTTGCACGGGATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCATCCACGTGGACGATTACGTTATCGGTCAGGAGCAGGCGAAAAAAGTGGTGGCGGTCGCGGTATACATGCATTACAAACTTCTGCGCAACGGc                                                                                       <  8:122364/147‑1 (MQ=255)
                                                           agaagTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCg                                                                                      <  1:367293/149‑1 (MQ=255)
                                                            gaagTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGa                                                                                     <  8:214756/149‑1 (MQ=255)
                                                            gaagTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGa                                                                                     <  7:56715/149‑1 (MQ=255)
                                                            gaagTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGa                                                                                     <  4:71614/149‑1 (MQ=255)
                                                            gaagTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGa                                                                                     <  6:133899/149‑1 (MQ=255)
                                                              agTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGta                                                                                                                     >  2:87966/1‑115 (MQ=255)
                                                              agTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGta                                                                                                                     <  1:87966/115‑1 (MQ=255)
                                                              agTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATa                                                                                   <  3:101453/149‑1 (MQ=255)
                                                              agTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATa                                                                                   <  3:155726/149‑1 (MQ=255)
                                                               gTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATAc                                                                                  >  8:38695/1‑149 (MQ=255)
                                                                 tGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGAAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACACACATTACAAACGTCTGCGCAACGGCGAAAc                                                                                  >  8:166652/1‑147 (MQ=255)
                                                                   cACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAg                                                                               <  1:232136/148‑1 (MQ=255)
                                                                    aCCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGc                                                                              >  5:130510/1‑148 (MQ=255)
                                                                      cGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGc                                                                                                                               <  3:24772/97‑1 (MQ=255)
                                                                      cGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGc                                                                                                                               >  4:24772/1‑97 (MQ=255)
                                                                      cGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAAt                                                                           >  6:64723/1‑149 (MQ=255)
                                                                       gCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATg                                                                          <  7:210853/149‑1 (MQ=255)
                                                                          tCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCg                                                                       >  5:50895/1‑149 (MQ=255)
                                                                               aaCGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACTTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTgg                                                                                                                                <  8:318267/87‑1 (MQ=255)
                                                                               aaCGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACTTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTgg                                                                                                                                >  7:318267/1‑87 (MQ=255)
                                                                                aCGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGt                                                                 <  4:152639/149‑1 (MQ=255)
                                                                                  gCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCaa                                                                            <  7:441284/136‑1 (MQ=255)
                                                                                  gCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCaa                                                                            >  8:441284/1‑136 (MQ=255)
                                                                                    aGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGc                                                                              <  8:104764/132‑1 (MQ=255)
                                                                                    aGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGc                                                                              >  7:104764/1‑132 (MQ=255)
                                                                                      tGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGc                                                            >  1:64129/1‑148 (MQ=255)
                                                                                           tACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGa                                                                                     >  7:193656/1‑118 (MQ=255)
                                                                                           tACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAg                                                       >  1:242960/1‑148 (MQ=255)
                                                                                              cGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCa                                                                                                             <  2:316586/91‑1 (MQ=255)
                                                                                              cGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCa                                                                                                             >  1:316586/1‑91 (MQ=255)
                                                                                              cGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAAc                                                   <  3:80417/149‑1 (MQ=255)
                                                                                               gACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACa                                                  >  3:15495/1‑149 (MQ=255)
                                                                                                 cgacgcATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGTCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACAtt                                                <  8:280340/146‑1 (MQ=255)
                                                                                                   ccgcATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGGCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTct                                              <  6:130510/149‑1 (MQ=255)
                                                                                                    cgcATGAAATTCGCAACCACCTGGACGCTTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTctg                                             <  5:64723/149‑1 (MQ=255)
                                                                                                       aTGAAATTCGCAACCACCTGGACGATTTCGTTATCGGGCAGGAGGAGGCGAAAAAAGTGCTGGGGGTCGCGGTATACAAGCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTGGAGTTGGGCAAAAGTAACATTctgc                                            <  1:236257/147‑1 (MQ=255)
                                                                                                       aTGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGAGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTctgct                                           >  6:305800/1‑148 (MQ=255)
                                                                                                       aTGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCACGACCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTCCGCAACGGCGATACCAGCAATCGCGTCGACTTGCGCAAAAGTAACATTcagcta                                          >  2:236257/1‑148 (MQ=255)
                                                                                                       aTGAAATTCGCAACCACCTGGACAATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACAtt                                                <  7:108810/143‑1 (MQ=255)
                                                                                                       aTGAAATTCGCAACCACCTGGACAATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACAtt                                                >  8:108810/1‑143 (MQ=255)
                                                                                                        tGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTctgct                                           >  6:293078/1‑147 (MQ=255)
                                                                                                          aaaTTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGAt                                        <  1:297670/148‑1 (MQ=255)
                                                                                                            aTTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCgg                                     >  8:131146/1‑149 (MQ=255)
                                                                                                             ttCGCAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGt                                    <  1:100053/149‑1 (MQ=255)
                                                                                                                 cAACCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGa                                <  2:327187/149‑1 (MQ=255)
                                                                                                                  aaCCACCTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGAc                               <  7:375470/149‑1 (MQ=255)
                                                                                                                    ccaccTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACaaa                                                                                                      >  8:137628/1‑76 (MQ=255)
                                                                                                                    ccaccTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACaaa                                                                                                      <  7:137628/76‑1 (MQ=255)
                                                                                                                     aaccTGGACGATTACGTTATCGGCCAGGAGCAGGCGTAAAAAGTGCTGGCGGTCGCGGGATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTAGACTAGGGCAAAAGTAACATTCTGCTGATCGGTCCGAcagt                            >  2:55905/2‑146 (MQ=255)
                                                                                                                      accTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGCTACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTccagatcgg                           >  3:75684/1‑142 (MQ=255)
                                                                                                                      accTGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTc                                   <  4:75684/141‑1 (MQ=255)
                                                                                                                         tGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACAt                                                 <  6:309718/124‑1 (MQ=255)
                                                                                                                         tGGACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACAt                                                 >  5:309718/1‑124 (MQ=255)
                                                                                                                          ggACGATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCg                       >  8:320164/1‑149 (MQ=255)
                                                                                                                              gATTACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTa                    >  3:212667/1‑148 (MQ=255)
                                                                                                                                ttACGTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTaaaa                 >  7:417135/1‑149 (MQ=255)
                                                                                                                                    gTTATCGGCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACgct             <  5:305800/149‑1 (MQ=255)
                                                                                                                                           gCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTgctggct      <  4:290881/149‑1 (MQ=255)
                                                                                                                                           gCCAGGAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTgctggct      <  2:64129/149‑1 (MQ=255)
                                                                                                                                               ggAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGaaa  <  5:304973/149‑1 (MQ=255)
                                                                                                                                               ggAGCAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGaaa  >  6:25209/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GTGTATATCTGCGACGAATGTGTTGATTTATGTAACGACATCATTCGCGAAGAGATTAAAGAAGTTGCACCGCATCGTGAACGCAGTGCGCTACCGACGCCGCATGAAATTCGCAACCACCTGGACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAA  >  NC_000913/457525‑457816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: