Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,100,923	A→G	S116P (TCA→CCA)	gnd ←	6‑phosphogluconate dehydrogenase, decarboxylating

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,100,923	0	A	G	100.0%	151.9 / NA	43	S116P (TCA→CCA)	gnd	6‑phosphogluconate dehydrogenase, decarboxylating
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/22);  total (21/22)

GTCAGGATCGGTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACA  >  NC_000913/2100799‑2101057                                                                                                                             |                                                                                                                                       gTCAGGATCGGTGCTACCAATTCATAGGCTTCTTTCTGGCTACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAgt                                                                                                                >  7:115355/1‑149 (MQ=255)           gTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCTTCTGCTGGAAGCTCACGATTACGACGAATAGTGTc                                                                                                             <  5:42798/142‑1 (MQ=255)           gTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCTTATGCTGGAAGCTCACGATTACGACGAATAGTGTc                                                                                                             >  6:42798/1‑142 (MQ=255)           gTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGgaag                                                                                                      <  6:119454/149‑1 (MQ=255)              cTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATa                                                                                                                  <  1:76928/134‑1 (MQ=255)              cTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATa                                                                                                                  >  2:76928/1‑134 (MQ=255)              cTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGgaagaa                                                                                                    <  3:94553/148‑1 (MQ=255)                        aTAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTacca                                                                                          <  2:103980/148‑1 (MQ=255)                             cttcttTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATc                                                                                     >  1:160007/1‑148 (MQ=255)                              ttctttctGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAGGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCaa                                                                                   >  4:84923/1‑149 (MQ=255)                              ttctttctGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCAc                                                                                                                                >  5:153473/1‑104 (MQ=255)                              ttctttctGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCAc                                                                                                                                <  6:153473/104‑1 (MQ=255)                                ctttctGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCaa                                                                                   <  2:160007/147‑1 (MQ=255)                                         caccaGGCATAATAGAAGGACCTATCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGtct                                                                        >  8:15209/1‑149 (MQ=255)                                                       gAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAat                                                                                  <  4:114319/125‑1 (MQ=255)                                                       gAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAat                                                                                  >  3:114319/1‑125 (MQ=255)                                                       gAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGata                                                          >  3:47912/1‑149 (MQ=255)                                                               tttCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATg                                                        <  6:34455/143‑1 (MQ=255)                                                               tttCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATg                                                        >  5:34455/1‑143 (MQ=255)                                                                 tCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAGGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGa                                                  <  3:84923/147‑1 (MQ=255)                                                                 tCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAg                                                 <  8:115355/148‑1 (MQ=255)                                                                    gcgcCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGaa                                             <  7:15209/149‑1 (MQ=255)                                                                          ccTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGa                                                  <  4:14863/138‑1 (MQ=255)                                                                          ccTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGa                                                  >  3:14863/1‑138 (MQ=255)                                                                          ccTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCaa                                         >  5:123732/1‑147 (MQ=255)                                                                          ccTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCaa                                         >  5:148755/1‑147 (MQ=255)                                                                           ctctTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATag                                      <  6:123732/149‑1 (MQ=255)                                                                            tctTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATag                                      >  5:118024/1‑148 (MQ=255)                                                                                 aCCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCAt                                >  5:125215/1‑149 (MQ=255)                                                                                 aCCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCAt                                >  7:35423/1‑149 (MQ=255)                                                                                    gCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCg                             <  8:35423/149‑1 (MQ=255)                                                                                     ccAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGt                            >  8:137283/1‑149 (MQ=255)                                                                                             acCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCAc                    <  4:47912/149‑1 (MQ=255)                                                                                                 gTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTAt                                                                 <  1:101209/100‑1 (MQ=255)                                                                                                 gTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTAt                                                                 >  2:101209/1‑100 (MQ=255)                                                                                                       aTGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATagca                                    <  4:117006/123‑1 (MQ=255)                                                                                                       aTGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATagca                                    >  3:117006/1‑123 (MQ=255)                                                                                                                aaGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACa  <  6:148755/148‑1 (MQ=255)                                                                                                                aaGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACa  <  6:118024/148‑1 (MQ=255)                                                                                                                      tctGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTAt                                                                 <  3:151232/79‑1 (MQ=255)                                                                                                                      tctGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTAt                                                                 >  4:151232/1‑79 (MQ=255)                                                                                                                      tctGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGa                                                  <  7:94370/94‑1 (MQ=255)                                                                                                                      tctGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGa                                                  >  8:94370/1‑94 (MQ=255)                                                                                                                             |                                                                                                                                       GTCAGGATCGGTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACA  >  NC_000913/2100799‑2101057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑