Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,178,453 Δ1 bp 5.6% coding (1134/1245 nt) lolE → lipoprotein‑releasing system transmembrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,178,4530G. 5.6% 244.7 / 7.0 71coding (1134/1245 nt)lolElipoprotein‑releasing system transmembrane protein
Reads supporting (aligned to +/- strand):  ref base G (38/29);  new base . (2/2);  total (40/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.85e-01

CTGTGTGGTGTGATTATCGGCGTAGTGGTTTCACTGCAACTTACCCCGATTATTGAGTGGATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATATTGACCCTGCGCGAGTCCTTAGCGGCCAGTAAAGGCAGTACATTAAAACAAGGAGCGGCAATG  >  NC_000913/1178313‑1178595
                                                                                                                                            |                                                                                                                                              
cTGTGTGGTGTGATTATCGGCGTAGTGGTTTCACTGCAACTTACCCCGATTATTGAGTGGATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGtctt                                                                                                                                        >  8:254316/1‑149 (MQ=255)
   tgtggtgtgATTATCGGCGTAGTGGTTGCACTGCAACTTACCCCGATTATTGAGTGGATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGtcttct                                                                                                                                      <  8:299259/148‑1 (MQ=255)
          tgATTATCGGCGTAGTGGTTTCACTGCAACTTACCCCGATTATTGAGTGGATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTg                                                                                                                              >  3:291042/1‑149 (MQ=255)
            aTTATCGGCGTAGTGGTTTCACTGCAACTTACCCCGATTATTGAGTGGATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTgg                                                                                                                             <  8:70938/148‑1 (MQ=255)
                  ggCGTAGTGGTTTCACTGCAACTTACCCCGATTATTGAGTGGATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTTTTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTTCGTACTGGTCACTGc                                                                                                                      <  4:184561/149‑1 (MQ=255)
                        gTGGTTTCACTGCAACTTACCCCGATTATTGAGTGGATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCAttgtt                                                                                                                <  8:133016/149‑1 (MQ=255)
                        gTGGTTTCACTGCAACTTACCCCGATTATTGAGTGGATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCAttgtt                                                                                                                >  4:197548/1‑149 (MQ=255)
                           gTTTCACTGCAACTTACCCCGATTATTGAGTGGATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCt                                                                                                             <  7:93169/149‑1 (MQ=255)
                            tttCACTGCAACTTACCCCGATTATTGAGTGGATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCt                                                                                                             >  6:59109/1‑148 (MQ=255)
                              tCACTGCAACTTACCCCGATTATTGAGTGGATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGa                                                                                                           <  3:1309/148‑1 (MQ=255)
                                  tGCAACTTACCCCGATTATTGAGTGGATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCt                                                                                                             >  7:326152/1‑142 (MQ=255)
                                  tGCAACTTACCCCGATTATTGAGTGGATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCt                                                                                                             <  8:326152/142‑1 (MQ=255)
                                  tGCAACTTACCCCGATTATTGAGTGGATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCtt                                                                                                      >  3:153462/1‑149 (MQ=255)
                                     aaCTTACCCCGATTATTGAGTGGATTGAAAAGTTGATCTGTCATCAGTTCCTCTCCATCGATATCTATTTTATTTATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACTTACTGGCCACAGCATTGTTGCTGAGTCTTTTg                                                                                                   <  7:357057/149‑1 (MQ=255)
                                     aaCTTACCCCGATTATTGAGTGGATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTg                                                                                                   <  5:127297/149‑1 (MQ=255)
                                      aCTTACCCCGATTATTGAGTGGATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTgg                                                                                                  >  4:106302/1‑149 (MQ=255)
                                       cTTACCCCGATTATTGAGTGGATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGc                                                                                                 <  2:98790/149‑1 (MQ=255)
                                           ccccGATTATTGAGTGGATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGt                                                                                             <  7:254316/149‑1 (MQ=255)
                                            cccGATTATTGAGTGGATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGtt                                                                                            >  1:40173/1‑149 (MQ=255)
                                            cccGATTATTGAGTGGATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGtt                                                                                            <  7:56649/149‑1 (MQ=255)
                                            cccGATTATCGAGTGGATTGAAAAGTTGATCGGTCACCAGTACCACTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGTAATTGCACTGGCAGCACGTATACTAAGTACTGGTCACAGCATTGTTTCTGAGTCTTTTGGCAAGtt                                                                                            >  5:89886/1‑149 (MQ=255)
                                              cGATTATTGAGTGGATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTgg                                                                                          <  3:231127/149‑1 (MQ=255)
                                              cGATTATTGAGTGGATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTgg                                                                                          >  6:252695/1‑149 (MQ=255)
                                              cGATTATTGAGTGGATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTgg                                                                                          >  1:62911/1‑149 (MQ=255)
                                               gATTATTGAGTGGATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGt                                                                                         <  1:341369/149‑1 (MQ=255)
                                                     tGAGTGGATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCcgg                                                                                   >  1:398130/1‑149 (MQ=255)
                                                        gTGGATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTTTTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTg                                                                                           <  2:294994/138‑1 (MQ=255)
                                                        gTGGATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTg                                                                                           >  1:294994/1‑138 (MQ=255)
                                                        gTGGATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCcggctc                                                                                <  2:40173/149‑3 (MQ=255)
                                                         tGGATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCcggcgcg                                                                               >  3:59300/1‑149 (MQ=255)
                                                            aTTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCcggcgcggcg                                                                            >  1:20642/1‑149 (MQ=255)
                                                             ttGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCggcgcggcgc                                                                           >  7:86829/1‑149 (MQ=255)
                                                              tGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGgcgcggcgcg                                                                          >  1:33401/1‑149 (MQ=255)
                                                               gAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGgcgcgc                                                                         >  1:217688/1‑149 (MQ=255)
                                                                aaaaGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGgcgcgc                                                                         >  8:149405/1‑148 (MQ=255)
                                                                 aaaGTTGATCGGTCTCCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCGATCGGAATTGCTCTGGCTGGACGTCTTCTACGTACTGGTCACATCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCa                                                                       <  6:89886/149‑1 (MQ=255)
                                                                 aaaGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCa                                                                       <  5:252695/149‑1 (MQ=255)
                                                                   aGTGGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCCTCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGGCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGt                                                                     <  3:241400/149‑1 (MQ=255)
                                                                         tCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATAtt                                                               >  2:209896/1‑149 (MQ=255)
                                                                         tCGGTCATCAGTTCCGCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATAtt                                                               <  8:181800/149‑1 (MQ=255)
                                                                           ggTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTCGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATATTGa                                                             <  3:95646/149‑1 (MQ=255)
                                                                           ggTCATCAGTTCCTCTCCAGCGATAGCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGGCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATATTGa                                                             <  3:106302/149‑1 (MQ=255)
                                                                            gTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATATTGAc                                                            >  2:330755/1‑149 (MQ=255)
                                                                               atcaGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATATTGACCCt                                                         >  6:253859/1‑149 (MQ=255)
                                                                                tcaGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATATTGACCCTg                                                        >  6:88718/1‑149 (MQ=255)
                                                                                 caGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATATTGACCCTgcg                                                      >  8:167861/1‑149 (MQ=255)
                                                                                 caGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATATTGACCCTgcg                                                      >  8:162513/1‑149 (MQ=255)
                                                                                   gTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATATTGACCCTgcgc                                                     >  3:238041/1‑149 (MQ=255)
                                                                                          tcCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATATTGACCCTGCGCGAGTCCt                                              >  8:281531/1‑149 (MQ=255)
                                                                                            cAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATATTGACCCTGCGCGAGTCCt                                              <  5:59109/147‑1 (MQ=255)
                                                                                            cAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATATTGACCCTGCGCGAGTCCTTAg                                           <  7:162513/149‑1 (MQ=255)
                                                                                            cAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATATTGACCCTGCGCGAGTCCTTAg                                           <  7:167861/149‑1 (MQ=255)
                                                                                              gCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATATTGACCCTGCGCGAGTCCTTAGc                                          >  7:121871/1‑149 (MQ=255)
                                                                                              gCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATATTGACCCTGCGCGAGTCCTTAGc                                          <  1:209896/149‑1 (MQ=255)
                                                                                               cGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATATTGACCCTGCGCGAGTCCTTAGCg                                         <  4:291042/149‑1 (MQ=255)
                                                                                                 atatCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATATTGACCCTGCGCGAGTCCTTAGCGGc                                       <  2:398130/149‑1 (MQ=255)
                                                                                                               gATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATATTGACCCTGCGCGAGTCCTTAGCGGCCAGTAAAGGCAGt                          <  5:88718/148‑1 (MQ=255)
                                                                                                                aTTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATATTGACCCTGCGCGAGTCCTTAGCGGCCAGTAACGGCAGTa                         >  1:297652/1‑148 (MQ=255)
                                                                                                                aTTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATATTGACCCTGCGCGAGTCCTTAGCGGCCAGTAAAGGCAGTa                         <  7:149405/148‑1 (MQ=255)
                                                                                                                  ttCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGATGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCGAGTAATATTGACCATGCGCGAGTCCTTAGCGGCCAGTAAAGGCAGTActt                      >  6:261815/1‑147 (MQ=255)
                                                                                                                      tGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATATTGACCCTGCGCGAGTCCTTAGCGGCCAGTAAAGGCAGTACATTaa                   >  2:183864/1‑148 (MQ=255)
                                                                                                                      tGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATATTGACCCTGCGCGAGTCCTTAGCGGCCAGTAAAGGCAGTACATTaa                   >  8:297429/1‑148 (MQ=255)
                                                                                                                        ccATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATATTGACCCTGCGCGAGTCCTTAGCGGCCAGTAAAGGCAGTACGTTaaaa                 >  5:309640/1‑148 (MQ=255)
                                                                                                                          aTCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATATTGACCCTGCGCGAGTCCTTAGCGGCCAGTAAAGGCAGTAGATTAAAAc                >  4:105821/1‑147 (MQ=255)
                                                                                                                          aTCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATATTGACCCTGCGCGAGTCCTTAGCGGCCAGTAAAGGCAGTACATTAAAAc                >  5:163058/1‑147 (MQ=255)
                                                                                                                          aTCGGAATAGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATATTGACCCTGCGCGAGTCCTTAGCGGCCAGTAAAGGCAGTACATTAAAAc                >  4:256673/1‑147 (MQ=255)
                                                                                                                           tCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATATTGACCCTGCGCGAGTCCTTAGCGGCCAGTAAAGGCAGTACATTAAAACAAg             >  7:303024/1‑149 (MQ=255)
                                                                                                                           tCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGGCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATATTGACCCTGCGCGAGTCCTTAGCGGCCAGTAAAGGCAGTACATTAAAACAAg             <  6:90695/149‑1 (MQ=255)
                                                                                                                             ggAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATATTGACCCTGCGCGAGTCCTTAGCGGCCAGTAAAGGCAGTACATTAAAACAAg             >  8:864/1‑147 (MQ=255)
                                                                                                                                 ttGCACTGGCTGGACGTCTTCTACGTACTGGTCAAAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATATTGACCCTGCGCGAGTCCTTAGCGGCCAGTAAAGGCAGTACATTAAAACAAGGAGCgg       >  8:32797/1‑149 (MQ=255)
                                                                                                                                     aCTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATATTGACCCTGCGCGAGTCCTTAGCGGCCAGTAAAGGCAGTACATTAAAACAAGGAGCGGCAAt   >  7:153600/1‑149 (MQ=255)
                                                                                                                                       tggctggACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATATTGACCCTGCGCGAGTCCTTAGCGGCCAGTAAAGGCAGTACATTAAAACAAGGAGCGGCAATg  >  6:271396/1‑148 (MQ=255)
                                                                                                                                       tggctggACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATATTGACCCTGCGCGAGTCCTTAGCGGCCAGTAAAGGCAGTACATTAAAACAAGGAGCGGCAATg  >  6:70730/1‑148 (MQ=255)
                                                                                                                                         gctggACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATATTGACCCTGCGCGAGTCCTTAGCGGCCAGTAAAGGCAGTACATTAAAACAAGGAGCg        <  8:214526/140‑1 (MQ=255)
                                                                                                                                         gctggACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATATTGACCCTGCGCGAGTCCTTAGCGGCCAGTAAAGGCAGTACATTAAAACAAGGAGCg        >  7:214526/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                              
CTGTGTGGTGTGATTATCGGCGTAGTGGTTTCACTGCAACTTACCCCGATTATTGAGTGGATTGAAAAGTTGATCGGTCATCAGTTCCTCTCCAGCGATATCTATTTTATTGATTTCCTGCCATCGGAATTGCACTGGCTGGACGTCTTCTACGTACTGGTCACAGCATTGTTGCTGAGTCTTTTGGCAAGTTGGTATCCGGCGCGGCGCGCCAGTAATATTGACCCTGCGCGAGTCCTTAGCGGCCAGTAAAGGCAGTACATTAAAACAAGGAGCGGCAATG  >  NC_000913/1178313‑1178595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: