Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,378,757 T→C 5.1% D63G (GAC→GGC)  ampC ← penicillin‑binding protein; beta‑lactamase, intrinsically weak

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,378,7570TC 5.1% 299.2 / 2.6 96D63G (GAC→GGC) ampCpenicillin‑binding protein; beta‑lactamase, intrinsically weak
Reads supporting (aligned to +/- strand):  ref base T (44/46);  new base C (2/3);  total (46/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCAGGCCAGTATTTTGTTGTGGGATCGCTTAACTTGATTTCCCCTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGA  >  NC_000913/4378612‑4378905
                                                                                                                                                 |                                                                                                                                                    
ttCAGGCCAGTATTTTGTTGTGGGATCGCTTAACTTGATTTCCCCTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCg                                                                                                                                                   >  5:166931/1‑149 (MQ=255)
   aGGCCAGTATTTTGTTGTGGGATCGCTTAACTTGATTTCCCCTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCa                                                                                                                                                 <  5:104547/148‑1 (MQ=255)
     gCCAGTATTTTGTTGTGGGATCGCTTAACTTGATTTCCCCTCGAGCAATAGCGTCGCCACCAAGCCCGCCAGTAAATGTTTTGCTGACCGAACCTAAGTCAAACAAGGTTTGCTGTGTGCCGGGCTGCTTTTTGGCGATGTCCGCAtcg                                                                                                                                              >  5:117649/1‑147 (MQ=255)
     gCCAGTATTTTGTTGTGGGATCGCTTAACTTGATTTCCCCTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAg                                                                                                                                              <  2:388192/149‑1 (MQ=255)
     gCCAGTATTTTGTTGTGGGATCGCTTAACTTGATTTCACCTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACACCGTTTGCTGTGTGACGGGATGGTTTTTGGCGATGTCCGCAtcg                                                                                                                                              >  4:187153/1‑147 (MQ=255)
       cAGTATTTTGTTGTGGGATCGCTTAACTTGATTTCCCCTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGcc                                                                                                                                            >  3:83827/1‑149 (MQ=255)
       cAGTATTTTGTTGTGGGATCGCTTAACTTGATTTCCCCTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGcc                                                                                                                                            >  7:201621/1‑149 (MQ=255)
         gTATTTTGCTGGGGGATCGCTTACCTTGATTTCCCCTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGcccc                                                                                                                                          <  4:89727/149‑1 (MQ=255)
          tcttttGGTGGGGGATCGCTTAACTTGATTTCCCCTCGAGCAAGAGCGTCGCCACCAAGCACGCCAGTAACTGTGTTGCTGACCGAACCTCACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTGGGCGATGGCCGCATAGCCCCa                                                                                                                                         <  5:260026/147‑1 (MQ=255)
           aTTTTGTTGTGGGATCGCTTAACTTGATTTCCCCTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAg                                                                                                                                        >  6:174330/1‑149 (MQ=255)
           aTTTTGTTGTGGGATCGCTTAACTTGATTTCCCCTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGGTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAg                                                                                                                                        >  6:174333/1‑149 (MQ=255)
            ttttGTTGTGGGATCGCTTAACTTGATTTCCCCTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAg                                                                                                                                        <  4:83827/148‑1 (MQ=255)
            ttttGTTGTGGGATCGCTTAACTTGATTTCCCCTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCGGCTTTTTGGCGATGTCCGCATAGCCCCAg                                                                                                                                        <  5:142767/148‑1 (MQ=255)
            ttttGTTGTGGGATCGCTTAACTTGATTTCCCATCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAg                                                                                                                                        <  2:60616/148‑1 (MQ=255)
                 ttgtGGGATCGCTTAACTTGATTTCCCCTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGTCGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAg                                                                                                                                  <  7:169314/149‑1 (MQ=255)
                 ttgtGGGATCGCTTAACTTGATTTCCCCTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTaaa                                                                                                                                   <  2:346576/148‑1 (MQ=255)
                 ttgtGGGATCGCTTAACTTGATTTCCCCTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTaaa                                                                                                                                   <  6:166931/148‑1 (MQ=255)
                   gtgGGATCGCTTAACTTGATTTCCCCTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGt                                                                                                                                 >  7:291074/1‑148 (MQ=255)
                   gtgGGATCGCTTAACTTGATTTCCCCTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGt                                                                                                                                 >  3:324398/1‑148 (MQ=255)
                   gtgGGATCGCTTAACTTGATTTCCCCTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGt                                                                                                                                 <  7:124556/148‑1 (MQ=255)
                      ggATCGCTTAACTTGATTTCCCCTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGtaat                                                                                                                              <  8:291074/148‑1 (MQ=255)
                           gCTTAACTTGATTTCCCCTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGt                                                                                                                         <  2:361020/148‑1 (MQ=255)
                           gCTTAACTTGATTTCCCCTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGt                                                                                                                         <  3:208716/148‑1 (MQ=255)
                               aaCTTGATTTCCCCTCGAGCAATAGCGTCGCCACAAAGCACGCCAGTAAATGTTTTGCTGACCGAACATAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCACAGGTAAAGTAATAAGGTTTAcc                                                                                                                    >  1:28203/1‑149 (MQ=255)
                                aCTTGATTTCCCCTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTAccc                                                                                                                   <  7:94174/149‑1 (MQ=255)
                                aCTTGATTTCCCCTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTAcc                                                                                                                    <  4:67391/148‑1 (MQ=255)
                                aCTTGATTTCCCCTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTAcc                                                                                                                    <  4:184418/148‑1 (MQ=255)
                                  ttGATTTCCCCTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCt                                                                                                                  >  8:193332/1‑148 (MQ=255)
                                      tttCCCCTCGAGCAATAGCGGCGCCACCAAGCACGACTGTAAATGTTTTGCTGACCGTACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATaa                                                                                                             <  6:4766/149‑1 (MQ=255)
                                            cTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGttt                                                                                                                       <  3:67630/133‑1 (MQ=255)
                                            cTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGttt                                                                                                                       >  4:67630/1‑133 (MQ=255)
                                            cTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTacc                                                                                                       >  7:177271/1‑149 (MQ=255)
                                            cTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTacc                                                                                                       <  1:158836/149‑1 (MQ=255)
                                             tCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTaccg                                                                                                      >  6:271531/1‑149 (MQ=255)
                                               gAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTaccgcc                                                                                                    <  6:207982/149‑1 (MQ=255)
                                               gAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTaccgc                                                                                                     >  6:239109/1‑148 (MQ=255)
                                               gAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTaccgc                                                                                                     >  5:219965/1‑148 (MQ=255)
                                                             ccaccaAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCATGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCgg                                                                                      <  4:166563/149‑1 (MQ=255)
                                                             ccaccaAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCgg                                                                                      <  4:345222/149‑1 (MQ=255)
                                                             ccaccaAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCgg                                                                                      <  4:215466/149‑1 (MQ=255)
                                                              caccaAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAgg                                                                                                                          >  1:373454/1‑112 (MQ=255)
                                                              caccaAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAgg                                                                                                                          <  2:373454/112‑1 (MQ=255)
                                                              caccaAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCgg                                                                                      >  2:273176/1‑148 (MQ=255)
                                                              caccaAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCgg                                                                                      >  5:23109/1‑148 (MQ=255)
                                                              caccaAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCgg                                                                                      >  8:353493/1‑148 (MQ=255)
                                                               accaAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGa                                                                                    <  5:271531/149‑1 (MQ=255)
                                                                ccaAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAg                                                                                                                                        >  3:148695/1‑96 (MQ=255)
                                                                ccaAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAg                                                                                                                                        <  4:148695/96‑1 (MQ=255)
                                                                   aGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCtt                                                                                <  8:177271/149‑1 (MQ=255)
                                                                      aCGCCAGTAAATGTGTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTttg                                                                             <  5:154232/149‑1 (MQ=255)
                                                                       cGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTttgt                                                                            <  5:239109/149‑1 (MQ=255)
                                                                        gCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTAcc                                                                                                                    <  6:264160/108‑1 (MQ=255)
                                                                        gCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTAcc                                                                                                                    >  5:264160/1‑108 (MQ=255)
                                                                        gCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTAgtgtt                                                                           >  5:264906/1‑149 (MQ=255)
                                                                            gTAAATGTTTTGCTGACAGAACCTAACTCAAACAACGTTAGCTGAGTGACGGGCTCCTTTTTGCCCATCTCCGCATAGCCCCAGCTAAAGTAATAAGGTTAACCCTGATAAAATACCGCCACCGCCATACACCGGATCTATTGTTGcac                                                                       >  4:75238/1‑147 (MQ=255)
                                                                               aaTGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCtat                                                                    <  5:174330/149‑1 (MQ=255)
                                                                               aaTGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTCGCGATGTCCGCATAGCCCCAGGTAAAGTAATAACGATTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCtat                                                                    <  5:174333/149‑1 (MQ=255)
                                                                                aTGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCtat                                                                    >  8:216021/1‑148 (MQ=255)
                                                                                 tGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATCCCCGGGAGCTTTTGTTGCTCtat                                                                    >  8:381601/1‑147 (MQ=255)
                                                                                 tGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCtat                                                                    >  7:261839/1‑147 (MQ=255)
                                                                                   ttttGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGc                                                                <  1:36371/149‑1 (MQ=255)
                                                                                         tGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGt                                                           >  7:186681/1‑148 (MQ=255)
                                                                                          gACCGAACCTAACTCAAACAACGTTTGCTGTGTGAAGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCTCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTaa                                                         >  6:20889/1‑149 (MQ=255)
                                                                                           aCCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAAt                                                        >  4:116433/1‑149 (MQ=255)
                                                                                           aCCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACACGGGATCTTTTGTTGCTCTATAAGCGGGGTAAt                                                        >  4:186748/1‑149 (MQ=255)
                                                                                               aaCCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATtgtg                                                    <  6:219965/149‑1 (MQ=255)
                                                                                                aCCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTttgttg                                                                          <  4:338157/126‑1 (MQ=255)
                                                                                                aCCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTttgttg                                                                          >  3:338157/1‑126 (MQ=255)
                                                                                                aCCTAACTCAAACAACGTTTGCTGTGTGAAGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGc                                                   <  1:202311/149‑1 (MQ=255)
                                                                                                    aaCTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATg                                               >  2:149718/1‑149 (MQ=255)
                                                                                                      cTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGca                                             >  8:295740/1‑149 (MQ=255)
                                                                                                      cTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGca                                             >  7:229435/1‑149 (MQ=255)
                                                                                                       tCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTAc                                                                                                                     <  5:235961/76‑1 (MQ=255)
                                                                                                           acaacGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAata                                        <  4:324398/149‑1 (MQ=255)
                                                                                                           acaacGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAata                                        >  7:199013/1‑149 (MQ=255)
                                                                                                                gTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGtt                                   <  8:201621/149‑1 (MQ=255)
                                                                                                                gTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCATCGCACAattacccc                                   <  2:139365/149‑7 (MQ=255)
                                                                                                                   tGCTGTGTGACCGGCTGCGCTCTGGCGATGCCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATAACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTAAAAGCGGGGTAATTGTGCGATACACAATATCGCTGa                                 <  2:315500/148‑1 (MQ=255)
                                                                                                                     cTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGAttt                              <  3:232027/149‑1 (MQ=255)
                                                                                                                      tgtgtgACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATttg                             >  7:33792/1‑149 (MQ=255)
                                                                                                                       gtgtgACGGGCTGCTTTTTGGCGATGCCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACaa                                          <  5:20209/135‑1 (MQ=255)
                                                                                                                       gtgtgACGGGCTGCTTTTTGGCGATGCCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACaa                                          <  5:15284/135‑1 (MQ=255)
                                                                                                                       gtgtgACGGGCTGCTTTTTGGCGATGCCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACaa                                          >  6:20209/1‑135 (MQ=255)
                                                                                                                       gtgtgACGGGCTGCTTTTTGGCGATGCCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACaa                                          >  6:15284/1‑135 (MQ=255)
                                                                                                                        tgtgACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATttgt                            <  7:353493/148‑1 (MQ=255)
                                                                                                                          tgACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATttgtt                           >  2:342968/1‑147 (MQ=255)
                                                                                                                          tgACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGa                         <  4:164580/149‑1 (MQ=255)
                                                                                                                            aCGTGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAgg                       <  3:84203/149‑1 (MQ=255)
                                                                                                                            aCGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAgg                       >  6:113562/1‑149 (MQ=255)
                                                                                                                                 ctgctTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGgcag                  >  3:18287/1‑149 (MQ=255)
                                                                                                                                  tgctTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGgcag                  >  3:235849/1‑148 (MQ=255)
                                                                                                                                  tgctTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGgcag                  >  1:223206/1‑148 (MQ=255)
                                                                                                                                           gagATGTCCGCATAGCCCCAGGTAAAGTAATAAGTTTTACCCTGAAATATTACCGCCACCCCCATACCGCGGATCTTTTGTTGCCCTATACGCGGGGTAATTGGGCGAACCACAATATCGTTGATTTGTTGAGGGGCAGCAAATCTgg         <  2:28203/146‑1 (MQ=255)
                                                                                                                                            cGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAg       <  8:186681/149‑1 (MQ=255)
                                                                                                                                             gATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATAGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAg       >  1:59133/1‑148 (MQ=255)
                                                                                                                                                gTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGc      >  8:156275/1‑146 (MQ=255)
                                                                                                                                                 tCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGa        <  3:300310/143‑1 (MQ=255)
                                                                                                                                                 tCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGa        >  4:300310/1‑143 (MQ=255)
                                                                                                                                                 tCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAaga  <  7:193332/149‑1 (MQ=255)
                                                                                                                                                 tCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAaga  <  1:149718/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
TTCAGGCCAGTATTTTGTTGTGGGATCGCTTAACTTGATTTCCCCTCGAGCAATAGCGTCGCCACCAAGCACGCCAGTAAATGTTTTGCTGACCGAACCTAACTCAAACAACGTTTGCTGTGTGACGGGCTGCTTTTTGGCGATGTCCGCATAGCCCCAGGTAAAGTAATAAGGTTTACCCTGATAAATTACCGCCACCGCCATACCCGGGATCTTTTGTTGCTCTATAAGCGGGGTAATTGTGCGATGCACAATATCGTTGATTTGTTGAGGGGCAGCAAATGTGGAGCAAGA  >  NC_000913/4378612‑4378905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: