Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 908,975 G→T 6.9% A107D (GCC→GAC)  ltaE ← L‑allo‑threonine aldolase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913908,9750GT 6.9% 207.0 / 3.1 67A107D (GCC→GAC) ltaEL‑allo‑threonine aldolase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base G (25/37);  new base T (2/3);  total (27/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GGTAAATTCCCATGCTTCTTTCAGGTATTCCCGCGGCAACACTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTCGCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAG  >  NC_000913/908827‑909119
                                                                                                                                                    |                                                                                                                                                
ggTAAATTCCCATGCTTCTTTCAGGTATTCCCGCGGCAACACTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCgg                                                                                                                                                  <  1:369895/149‑1 (MQ=255)
     aTTCCCATGCTTCATTCAGGTATTCCCGCGGCAACACATTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATGTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGcc                                                                                                                                             <  1:300843/149‑1 (MQ=255)
     aTTCCCATGCGTCTATCAGGTATACCCGCGGCAACACTTTGCCGTTGTGGGTGCTTTCCAGACTGTGTATTTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGcc                                                                                                                                             <  1:233953/149‑1 (MQ=255)
         ccATGCTTCTTTCAGGTATTCCCGCGGCAACACTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGt                                                                                                                                         <  6:285126/149‑1 (MQ=255)
            tGCTTCTTTCAGGTATTCCCGCGGCAACACTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTa                                                                                                                                      <  2:12042/149‑1 (MQ=255)
             gCTTCTTTCAGGTATTCCCGCGGCAACACTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGTCAGCCGCGTCTAt                                                                                                                                     >  7:376229/1‑149 (MQ=255)
              cttcttTCAGGTATTCCCGCGGCAACACTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTAt                                                                                                                                     <  2:240260/148‑1 (MQ=255)
                    tCAGGTTTTCGCGCGGCAACACTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATTTCGTCGGGGTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTg                                                                                                                               <  5:255091/148‑1 (MQ=255)
                      aGGTATTCCCGCGGCAACACTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCgg                                                                                                                            <  4:202041/149‑1 (MQ=255)
                      aGGTATTCCCGAGGCAACACTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGTTATCGTCGGGTTTGATTTTCATAGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCgg                                                                                                                            >  1:294237/1‑149 (MQ=255)
                       ggTATTCCCGCGGCAACACTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACCTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGt                                                                                                                           <  8:9743/149‑1 (MQ=255)
                        gTATTCCCGCGGCAACACTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCTTCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGtt                                                                                                                          >  1:242623/1‑149 (MQ=255)
                        gTATTCCCGCGGCAACACTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGtt                                                                                                                          <  8:32383/149‑1 (MQ=255)
                        gTATTCCCGCGGCAACACTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGtt                                                                                                                          <  8:1603/149‑1 (MQ=255)
                        gTATTCCCGCGGCAACACTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGtt                                                                                                                          <  2:298247/149‑1 (MQ=255)
                           ttCCCGCGGCAACACTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGa                                                                                                                        <  2:110921/148‑1 (MQ=255)
                           ttCCCGCGGCAACACTTTGCCGTTGTGGGTGTTTTCCAGACAGAGTAATTTGGTGTGGGCGAAATGGATATCGTCGGGTGTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGaa                                                                                                                       <  5:336419/149‑1 (MQ=255)
                              ccGCGGCAACACTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCcgc                                                                                                                                           <  8:41484/126‑1 (MQ=255)
                              ccGCGGCAACACTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCcgc                                                                                                                                           >  7:41484/1‑126 (MQ=255)
                              ccGCGGCAACACTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATAc                                                                                                                    >  1:325439/1‑149 (MQ=255)
                              ccGCGGCAACACTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATAc                                                                                                                    >  7:219668/1‑149 (MQ=255)
                              ccGAGGCAACACTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTAATCGCCACTTTTTAAAGCGGTAGCGTGCCGTCGGCATCCGCGTCTATGTGTTGCGGATGAATAc                                                                                                                    >  8:268621/1‑149 (MQ=255)
                               cgcgGCAACACTTTGCCGGTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACt                                                                                                                   <  1:152927/149‑1 (MQ=255)
                                 cgGCAACACTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGc                                                                                                                 <  8:97062/149‑1 (MQ=255)
                                 cgGCAACACTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGc                                                                                                                 <  4:312044/149‑1 (MQ=255)
                                   gCAACACTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGcc                                                                                                                >  4:159481/1‑148 (MQ=255)
                                    cAACACTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGcc                                                                                                                >  7:180583/1‑147 (MQ=255)
                                          tttGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTc                                                                                                                                        >  8:112690/1‑117 (MQ=255)
                                          tttGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTc                                                                                                                                        <  7:112690/117‑1 (MQ=255)
                                              ccGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCAAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACcgccgc                                                                                                    >  7:220175/1‑149 (MQ=255)
                                               cGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGATTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATTGGATGCAGTTGAATACTGCCCAGCACCGCcgcg                                                                                                   >  6:284592/1‑149 (MQ=255)
                                                    tgGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCAc                                                                                               <  2:146231/148‑1 (MQ=255)
                                                       gtgtTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATCTCGTCGGCTGTGATTTTCATCGCCACTTTATCCAGCGGGAGCGGGCCGGCGGCAGCCGCGTCTATGGGGTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGc                                                                                           <  1:397695/149‑1 (MQ=255)
                                                        tgtTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATAGACACTTTAACCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCGATGGGTTGCGGTTGAATAATGCCCAGCACCGCCGCGCCACCGGCt                                                                                          >  7:113846/1‑149 (MQ=255)
                                                               cAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGTCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACa                                                                                   <  7:268621/149‑1 (MQ=255)
                                                                  aCTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCa                                                                                                                                                >  8:110614/1‑85 (MQ=255)
                                                                  aCTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCa                                                                                                                                                <  7:110614/85‑1 (MQ=255)
                                                                  aCTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGa                                                                                 >  6:359069/1‑148 (MQ=255)
                                                                   cTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATa                                                                               <  5:51337/149‑1 (MQ=255)
                                                                   cTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTGTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATa                                                                               <  5:359069/149‑1 (MQ=255)
                                                                    tGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTTCCGTCTGCAACCGCGTCTATGGGTTTAGGTTGAATAATGCACAGCACCGACGCTCAACCGGCTTCAAACAGAAAg                                                                              >  3:93036/1‑149 (MQ=255)
                                                                     gAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGt                                                                             >  3:267025/1‑149 (MQ=255)
                                                                          aTTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATgc                                                                        <  6:102825/149‑1 (MQ=255)
                                                                               gTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGc                                                                   <  1:33807/149‑1 (MQ=255)
                                                                                  cGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCt                                                                 <  3:159481/148‑1 (MQ=255)
                                                                                    ggCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGTCAGCCGCGTCTATGGGTTGCTGTTGAATACTGCCCAGCACCGCCGCGCCACAGGCTTCAAACAGATAGTTATGCGCGGACTGAc                                                              <  8:376229/149‑1 (MQ=255)
                                                                                    ggCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGAc                                                              <  2:242623/149‑1 (MQ=255)
                                                                                    ggCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGAc                                                              <  8:219668/149‑1 (MQ=255)
                                                                                     gCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGAcc                                                             >  3:314795/1‑149 (MQ=255)
                                                                                       gAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGa                                                           >  2:70207/1‑149 (MQ=255)
                                                                                       gAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGa                                                           <  8:220175/149‑1 (MQ=255)
                                                                                                   gtcgGGCTTGATTATCATCGCCACTTTCTCCAGCGGTAGCGTGCCGCCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCtt                                               <  2:200241/149‑1 (MQ=255)
                                                                                                    tcgGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCCGCACCGCCGCGCCCCCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTc                                              >  4:224797/1‑149 (MQ=255)
                                                                                                           tGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTCGCcgcg                                        <  5:284592/148‑1 (MQ=255)
                                                                                                           tGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTCGCcgcg                                        <  8:113846/148‑1 (MQ=255)
                                                                                                           tGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTCGCCGCGt                                       >  2:27934/1‑149 (MQ=255)
                                                                                                                   agcGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTCGCCGCGTTCGCAGt                                <  6:196291/146‑1 (MQ=255)
                                                                                                                   aTCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTCGCCGCGTTCGCAGt                                <  5:319745/148‑1 (MQ=255)
                                                                                                                      gCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTCGCCGCGTTCGCAGTGACt                            >  2:184439/1‑149 (MQ=255)
                                                                                                                       ccACTTTATCCAGCGGTAGCGTGCCGTCGTCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATAct                                                  <  8:126984/126‑1 (MQ=255)
                                                                                                                       ccACTTTATCCAGCGGTAGCGTGCCGTCGTCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATAct                                                  >  7:126984/1‑126 (MQ=255)
                                                                                                                         aCTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTCGCCGCGTTCGCAGTGACTGAg                         <  1:27934/149‑1 (MQ=255)
                                                                                                                                ccAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTCGCCGCGTTCGCAGTGACTGAGCAGAGCg                  >  8:57818/1‑149 (MQ=255)
                                                                                                                                         gCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTAGAATACTGCCCTGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTCGCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGtt          <  2:294237/148‑1 (MQ=255)
                                                                                                                                               cGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTCGCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCt     >  4:147366/1‑147 (MQ=255)
                                                                                                                                               cGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGATATGCGCGGCCTGACCGACAATATACTCTTCGCCGCGTTCGc                                   <  8:144259/117‑1 (MQ=255)
                                                                                                                                               cGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGATATGCGCGGCCTGACCGACAATATACTCTTCGCCGCGTTCGc                                   >  7:144259/1‑117 (MQ=255)
                                                                                                                                                gTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTCGCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAg  >  5:84004/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
GGTAAATTCCCATGCTTCTTTCAGGTATTCCCGCGGCAACACTTTGCCGTTGTGGGTGTTTTCCAGACTGAGTAATTTGGTGCGGGCGAAATGGATATCGTCGGGTTTGATTTTCATCGCCACTTTATCCAGCGGTAGCGTGCCGTCGGCAGCCGCGTCTATGGGTTGCGGTTGAATACTGCCCAGCACCGCCGCGCCACCGGCTTCAAACAGATAGTTATGCGCGGCCTGACCGACAATATACTCTTCGCCGCGTTCGCAGTGACTGAGCAGAGCGACCAGGTTGGCCTGAG  >  NC_000913/908827‑909119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑